Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,912 G→T 7.0% intergenic (+111/+28) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,9120GT 7.0% 234.3 / 4.6 84intergenic (+111/+28)rplY/yejK50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (45/33);  new base T (2/4);  total (47/37)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCA  >  NC_000913/2282767‑2283060
                                                                                                                                                 |                                                                                                                                                    
cGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATc                                                                                                                                                  >  2:1633115/1‑150 (MQ=255)
 gAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCt                                                                                                                                                 <  2:6188/150‑1 (MQ=255)
   aGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTgg                                                                                                                                               <  1:1492582/150‑1 (MQ=255)
   aGCTGCACCACTTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCCCTCCCGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTgg                                                                                                                                               <  1:498591/150‑1 (MQ=255)
    gCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGc                                                                                                                                              <  1:1605419/150‑1 (MQ=255)
       gcagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTTTTGCTATTTTTTGCACGCCAGATGCGGCGTGAACGCCTTATCTGGcctg                                                                                                                                           <  1:1131571/150‑4 (MQ=255)
       gcagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGcctg                                                                                                                                           <  1:1326959/150‑4 (MQ=255)
       gcagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGcctg                                                                                                                                           <  1:810642/150‑4 (MQ=255)
       gcagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAGGCGTTCACGCCGCATCTGGCATTc                                                                                                                                          <  1:758902/150‑1 (MQ=255)
            acaTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                                                                                                      >  2:876773/1‑150 (MQ=255)
             caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                                     <  2:428348/150‑1 (MQ=255)
             caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                                     <  1:739205/150‑1 (MQ=255)
             caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                                     <  2:613667/150‑1 (MQ=255)
              aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGCTTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAaca                                                                                                                                    >  2:871927/1‑150 (MQ=255)
                cGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAAcaca                                                                                                                                  <  1:889285/150‑1 (MQ=255)
                 gACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaa                                                                                                                                 >  1:954488/1‑150 (MQ=255)
                      cgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAATTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCg                                                                                                                            <  1:252217/150‑1 (MQ=255)
                        ttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGAt                                                                                                                          <  2:276013/150‑1 (MQ=255)
                                     gCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAg                                                                                                             >  2:802979/1‑150 (MQ=255)
                                       tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTTTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGAc                                                                                                           >  2:1662516/1‑150 (MQ=255)
                                       tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGAc                                                                                                           >  1:394762/1‑150 (MQ=255)
                                        gAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACg                                                                                                          >  2:719715/1‑150 (MQ=255)
                                        gAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACg                                                                                                          >  2:497291/1‑150 (MQ=255)
                                          aTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTc                                                                                                        >  1:112236/1‑150 (MQ=255)
                                            cagatacaaaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCACCAGACGTCCg                                                                                                      >  1:63189/8‑150 (MQ=255)
                                             aGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCgg                                                                                                     >  2:1669966/1‑150 (MQ=255)
                                             aGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCgg                                                                                                     <  1:1382879/150‑1 (MQ=255)
                                             aGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCgg                                                                                                     >  2:796792/1‑150 (MQ=255)
                                                 gTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCt                                                                                                 >  2:830734/1‑150 (MQ=255)
                                                 gTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCt                                                                                                 >  1:527790/1‑150 (MQ=255)
                                                  caaaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTg                                                                                                >  2:245115/2‑150 (MQ=255)
                                                  caaaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGACCGGCGCTg                                                                                                >  2:870548/2‑150 (MQ=255)
                                                   aaaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGc                                                                                               >  1:1418486/1‑150 (MQ=255)
                                                   aaaaaaCCCCGCTCCGGCGGGGTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGTGTTCACGCCGCATCTGGCAGTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCCGCa                                                                                              >  1:1616931/1‑150 (MQ=255)
                                                   aaaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGc                                                                                               >  2:1718957/1‑150 (MQ=255)
                                                   aaaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATAACGGTATAGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGc                                                                                               >  2:1278350/1‑150 (MQ=255)
                                                    aaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCa                                                                                              >  1:1674897/1‑150 (MQ=255)
                                                    aaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCa                                                                                              >  2:1671321/1‑150 (MQ=255)
                                                    aaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCCCGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCa                                                                                              >  1:1566209/1‑150 (MQ=255)
                                                        aCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTg                                                                                          >  1:1161456/1‑150 (MQ=255)
                                                          cccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGt                                                                                        >  2:1134958/1‑150 (MQ=255)
                                                          cccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTCTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGt                                                                                        <  1:724133/150‑1 (MQ=255)
                                                              cTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTcgcg                                                                                    >  2:1698872/1‑150 (MQ=255)
                                                                    cggGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGGGCAAAtt                                                                              >  1:997411/1‑150 (MQ=255)
                                                                        gTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGCTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGGCCGGCGCTGCAATTGGTCGCGCAAATTcggc                                                                          <  1:1523280/150‑1 (MQ=255)
                                                                        gTTTTTTGTATCTGCAGATTATGCCTGAGTACGGTATTGCTATTTTTTGCAGGCCAGATACGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcggc                                                                          <  1:476059/150‑1 (MQ=255)
                                                                           ttttGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCggcggc                                                                       <  1:1734112/150‑1 (MQ=255)
                                                                              tGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTg                                                                    <  2:1161456/150‑1 (MQ=255)
                                                                              tGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTg                                                                    >  2:1464246/1‑150 (MQ=255)
                                                                               gTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGATCGCGCAAATTCGGCGGCGTGc                                                                   >  2:504004/1‑150 (MQ=255)
                                                                                 aTCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCt                                                                 <  2:1095367/150‑1 (MQ=255)
                                                                                  tCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCtt                                                                >  1:971709/1‑150 (MQ=255)
                                                                                    tGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTa                                                              >  2:1291284/1‑150 (MQ=255)
                                                                                    tGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTa                                                              <  2:112236/150‑1 (MQ=255)
                                                                                    tGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAAGACGGCGGCGTGccgtga                                                              >  2:305962/1‑146 (MQ=255)
                                                                                        gATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATgg                                                          <  2:1478453/150‑1 (MQ=255)
                                                                                         aTTATGCCTGATTACTGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGt                                                         <  1:1677519/150‑1 (MQ=255)
                                                                                             tGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAg                                                     >  1:1652772/1‑150 (MQ=255)
                                                                                              gCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAgg                                                    <  2:958789/150‑1 (MQ=255)
                                                                                              gCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAgg                                                    <  2:1533259/150‑1 (MQ=255)
                                                                                              gCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAgg                                                    <  1:876773/150‑1 (MQ=255)
                                                                                               ccTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGt                                                   >  2:1394891/1‑150 (MQ=255)
                                                                                                  gATTACGGTATTGCTATTTTTAGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTAtt                                                <  1:332372/150‑2 (MQ=255)
                                                                                                          tATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTg                                        <  2:954488/150‑1 (MQ=255)
                                                                                                          tATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTg                                        <  2:944683/150‑1 (MQ=255)
                                                                                                               cTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCAGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATcc                                   >  2:1107733/1‑150 (MQ=255)
                                                                                                                tATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATccc                                  >  1:968832/1‑150 (MQ=255)
                                                                                                                 aTTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCa                                 >  1:1426589/1‑150 (MQ=255)
                                                                                                                    ttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCagaa                              >  1:349821/1‑150 (MQ=255)
                                                                                                                      ttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCagaaga                            <  2:1169548/150‑1 (MQ=255)
                                                                                                                         cAGGCCAGATATGGCGTTCACGCCGCATCTGGCTTTCAGAACACAATACCGTTTAATTGCCGCGAGAGGTCCGGCGGTGCAATTGGTCGCGCATATTCGGCGGCGTGCCTTTTATGGTCAAGGTATGGGTTGCTGGATCCCAGAAGATGc                         <  2:431569/150‑1 (MQ=255)
                                                                                                                         cAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGc                         <  2:1187515/150‑1 (MQ=255)
                                                                                                                           ggCAAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAACCCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTTGTCGCGCTAATTCGGAGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGt                       <  2:863946/150‑1 (MQ=255)
                                                                                                                            gCCAGATGCGGCGTGAACGCCTTATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGtt                      >  2:1475946/1‑150 (MQ=255)
                                                                                                                            gCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGtt                      <  2:1136561/150‑1 (MQ=255)
                                                                                                                             ccAGATGCGGCGTGAACGCCTTATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTc                     >  1:1018058/1‑150 (MQ=255)
                                                                                                                              cAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGACGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCa                    >  2:1307943/1‑150 (MQ=255)
                                                                                                                                   aaGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTaa               >  2:357619/1‑150 (MQ=255)
                                                                                                                                    aGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAAc              >  1:233571/1‑150 (MQ=255)
                                                                                                                                     ggCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACa             >  2:1415911/1‑150 (MQ=255)
                                                                                                                                         ttCACGCCGCATCTGGCATTCAGAACACAAAACTGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcat         >  1:65206/1‑150 (MQ=255)
                                                                                                                                         ttCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCACCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcat         <  2:63189/150‑1 (MQ=255)
                                                                                                                                           cACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGGCAAGGGATCGGATGCTGGATCCAACAAGATGCGTTCACCTAACAgcatcg       >  2:124140/1‑150 (MQ=255)
                                                                                                                                            aCGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcatcgc      <  2:246749/150‑1 (MQ=255)
                                                                                                                                             cgccgcATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcatcgca     <  1:830734/150‑1 (MQ=255)
                                                                                                                                             cgccgcATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcatcgca     <  2:1664877/150‑1 (MQ=255)
                                                                                                                                             cgccgcATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGAGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcatcgca     >  2:669775/1‑150 (MQ=255)
                                                                                                                                                cgcATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAGTGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCa  >  2:542940/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCA  >  NC_000913/2282767‑2283060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: