Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,311,616 C→T 8.3% intergenic (‑82/+30) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,311,6160CT 8.3% 396.6 / 15.9 133intergenic (‑82/+30)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (55/66);  new base T (6/5);  total (62/71)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 136 total aligned reads displayed.

GATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTT  >  NC_000913/2311471‑2311764
                                                                                                                                                 |                                                                                                                                                    
   cGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGcc                                                                                                                                               >  1:1560930/1‑150 (MQ=255)
        ccaacAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTg                                                                                                                                          <  1:783411/150‑1 (MQ=255)
        ccaacAAAGAAGAGAGCGGCAGCCAGCAGTGCCACGCGGTTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTg                                                                                                                                          <  1:860326/150‑1 (MQ=255)
         caacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGt                                                                                                                                         >  1:589634/1‑150 (MQ=255)
         caacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGt                                                                                                                                         >  2:868128/1‑150 (MQ=255)
         caacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGt                                                                                                                                         <  2:365705/150‑1 (MQ=255)
          aacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGtt                                                                                                                                        >  1:576125/1‑150 (MQ=255)
          aacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGAAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCATTTGtt                                                                                                                                        >  2:1169243/1‑150 (MQ=255)
          aacaaAGAAGAGCGCGGAAGACAGCATTTCAACGCTGGTAAAGCTTATTTCCATACGTGATTATCCTCATGAGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGtt                                                                                                                                        >  2:230617/1‑150 (MQ=255)
           acaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGGCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTc                                                                                                                                       <  1:1692273/150‑1 (MQ=255)
            caaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTTAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTca                                                                                                                                      <  2:1212065/150‑2 (MQ=255)
            caaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                                                                      <  1:961512/150‑1 (MQ=255)
            caaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                                                                      >  1:1362537/1‑150 (MQ=255)
            caaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAAATGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCACGCAGGCCCTTTGTTCg                                                                                                                                      >  2:857843/1‑150 (MQ=255)
             aaaGAAGATCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTcat                                                                                                                                     <  1:1105089/150‑3 (MQ=255)
             aaaGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGa                                                                                                                                     >  1:1585230/1‑150 (MQ=255)
               agaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGata                                                                                                                                   <  1:739772/150‑1 (MQ=255)
                gaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTcattgt                                                                                                                                  <  1:1001072/150‑6 (MQ=255)
                  agagCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCa                                                                                                                                <  1:984588/150‑1 (MQ=255)
                    agCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAAt                                                                                                                              >  1:1269972/1‑150 (MQ=255)
                         ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCCCAGGCCCTTTGTTCGATATCAATCgagt                                                                                                                         >  2:63753/1‑149 (MQ=255)
                           cagccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGAtt                                                                                                                       >  2:3910/1‑150 (MQ=255)
                                agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTAGATCGAAAACAATCGAGATTACAAc                                                                                                                  >  2:1286658/1‑150 (MQ=255)
                                 gcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACCCCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACt                                                                                                                 >  2:1351135/1‑150 (MQ=255)
                                   agTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTgg                                                                                                               <  2:25857/150‑1 (MQ=255)
                                      gCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTaa                                                                                                            >  1:357943/1‑150 (MQ=255)
                                      gCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTaa                                                                                                            >  2:266366/1‑150 (MQ=255)
                                           gcgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAg                                                                                                       <  2:194587/150‑1 (MQ=255)
                                              ggTAAAGCTTATTTCCATACGTGATTATCCTCAAGCGAACGTTCGAAAGAGGACACCATAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAcc                                                                                                    <  1:634517/150‑1 (MQ=255)
                                                  aaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCaga                                                                                                <  1:1406262/150‑1 (MQ=255)
                                                      ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTa                                                                                            >  1:187937/1‑150 (MQ=255)
                                                          ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGAt                                                                                        <  1:14314/150‑1 (MQ=255)
                                                           tCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATg                                                                                       <  2:881565/150‑1 (MQ=255)
                                                            ccATTCGTGATTATGCTCATGCGAACGGTCTCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAATGGGCCCGCAGGCCCTTTGTTCTATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGt                                                                                      <  1:1286658/150‑1 (MQ=255)
                                                            ccATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGt                                                                                      <  1:135191/150‑1 (MQ=255)
                                                              aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTa                                                                                    >  1:794665/1‑150 (MQ=255)
                                                                aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATc                                                                                  <  2:594522/150‑1 (MQ=255)
                                                                 cGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                                 >  2:1700706/1‑150 (MQ=255)
                                                                 cGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                                 >  1:1311450/1‑150 (MQ=255)
                                                                 cGTGATTATCCTCATGCGAACGGGCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                                 <  1:1085429/150‑1 (MQ=255)
                                                                  gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAg                                                                                >  2:1282156/1‑150 (MQ=255)
                                                                  gTGATTATACTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAg                                                                                <  2:1313068/150‑1 (MQ=255)
                                                                   tGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgt                                                                               >  2:748611/1‑150 (MQ=255)
                                                                       tATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGTCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTg                                                                           <  2:386224/150‑1 (MQ=255)
                                                                       tATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTg                                                                           <  2:911292/150‑1 (MQ=255)
                                                                       tATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTg                                                                           >  1:1659228/1‑150 (MQ=255)
                                                                          ccTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTTAGTTTGTACGCTGAAAACAATTAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                        <  1:1417518/150‑1 (MQ=255)
                                                                            tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGcc                                                                      <  2:768626/150‑1 (MQ=255)
                                                                            tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGcc                                                                      <  1:768148/150‑1 (MQ=255)
                                                                             cATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGTGACAGCAGGCACTTTGTTCGATATCAATAGAGATTAGAACTGGTAAACCAGACACAGAGCTACGATGTTTTCAGTGTTGATGCCa                                                                     >  1:981593/1‑150 (MQ=255)
                                                                             cATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                                     <  1:972728/150‑1 (MQ=255)
                                                                               tGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGc                                                                   <  1:5182/150‑1 (MQ=255)
                                                                                     cGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACg                                                             <  2:1595841/150‑1 (MQ=255)
                                                                                     cGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACg                                                             <  2:357943/150‑1 (MQ=255)
                                                                                     cGGTCGCAAGAGTACAACAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACg                                                             <  2:593554/150‑1 (MQ=255)
                                                                                      ggTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAACCCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCAAGa                                                            >  2:285302/1‑150 (MQ=255)
                                                                                      ggTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGa                                                            >  2:145780/1‑150 (MQ=255)
                                                                                       gTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAg                                                           <  1:204654/150‑1 (MQ=255)
                                                                                        tCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGt                                                          <  1:1676965/150‑1 (MQ=255)
                                                                                         cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                         <  1:1021291/150‑1 (MQ=255)
                                                                                          gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGa                                                        >  1:510259/1‑150 (MQ=255)
                                                                                                gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                                  <  2:510259/150‑1 (MQ=255)
                                                                                                gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                                  <  2:104812/150‑1 (MQ=255)
                                                                                                 tACACCAAAAAACTTAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                 <  2:799073/150‑1 (MQ=255)
                                                                                                 tACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                 >  1:476551/1‑150 (MQ=255)
                                                                                                  acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTg                                                >  2:1313348/1‑150 (MQ=255)
                                                                                                  acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTg                                                <  2:1515187/150‑1 (MQ=255)
                                                                                                     acAAAAAACTTAGTTTGTACGATTAAAACAATCAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGATCTACGATTTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcg                                             <  2:470151/149‑1 (MQ=255)
                                                                                                      cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACTAGTGAACTGGTTgtcgt                                            >  1:709016/1‑150 (MQ=255)
                                                                                                       aaaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                           <  2:1205497/150‑1 (MQ=255)
                                                                                                        aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                          <  2:1349130/150‑1 (MQ=255)
                                                                                                        aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                          <  1:868128/150‑1 (MQ=255)
                                                                                                          aaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTACACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                        <  1:301199/150‑1 (MQ=255)
                                                                                                               taGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                                   <  1:375755/149‑1 (MQ=255)
                                                                                                                aGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTTCGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtt                                  <  1:230617/150‑1 (MQ=255)
                                                                                                                     gTACGGTGTAAACAATGAAAATAGGGCCCGCAGGCCCTTTGTTCTATATCATTCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                             <  2:869093/150‑1 (MQ=255)
                                                                                                                     gTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCTCGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                             >  1:650426/1‑150 (MQ=255)
                                                                                                                     gTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                             <  2:806431/150‑1 (MQ=255)
                                                                                                                      tACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATAAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCAAGCAGGTTGAtttt                            >  1:598969/1‑150 (MQ=255)
                                                                                                                        cGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCCGACGCAGCGGTACGAAGTTATCAGTGTTGATGGCAGCGTCACGAGTGACCTGGTTGTCGTCCAGCAGGTTGCTTTTGt                          >  1:1035771/1‑150 (MQ=255)
                                                                                                                        cGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGt                          <  2:794665/150‑1 (MQ=255)
                                                                                                                         gCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                         <  1:1313348/150‑1 (MQ=255)
                                                                                                                          cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                        <  1:266366/150‑1 (MQ=255)
                                                                                                                          cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                        >  2:705290/1‑150 (MQ=255)
                                                                                                                          cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                        <  1:1250800/150‑1 (MQ=255)
                                                                                                                               gatatcgAACAAAGGGCCTGCGGGCCCTTTTTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAc                   >  2:1348220/7‑150 (MQ=255)
                                                                                                                                 tatcgAACAAAGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCAAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCTGCAGGTTGATTTTGTAGTCAACGt                 >  2:841611/5‑150 (MQ=255)
                                                                                                                                 cAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGCGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                 >  1:115585/1‑150 (MQ=255)
                                                                                                                                 cAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCATTGTTGATGCCAGCGGCACGAGTGAACTGGTTCTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                 >  2:82517/1‑150 (MQ=255)
                                                                                                                                 cAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                 <  1:644063/150‑1 (MQ=255)
                                                                                                                                  atcgAACAAAGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                >  1:433318/4‑150 (MQ=255)
                                                                                                                                   aTGAAAAAAGGGCCCGCAGGCCCTTTGTTAGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg               >  1:1523834/1‑150 (MQ=255)
                                                                                                                                    tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg              >  1:726637/1‑150 (MQ=255)
                                                                                                                                     gAACAAAGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGCCCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt             >  1:628161/1‑150 (MQ=255)
                                                                                                                                     gAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt             >  1:910817/1‑150 (MQ=255)
                                                                                                                                           aGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt       >  2:1309713/1‑150 (MQ=255)
                                                                                                                                              gCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt    <  1:748611/150‑1 (MQ=255)
                                                                                                                                               cccGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt   >  1:1239300/1‑150 (MQ=255)
                                                                                                                                                ccGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTTAACTCGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt  <  2:938035/150‑1 (MQ=255)
                                                                                                                                                ccGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt  <  2:187937/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTT  >  NC_000913/2311471‑2311764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: