Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,313,159 T→A 5.2% noncoding (76/93 nt) micF → sRNA antisense regulator affecting ompF expression, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,313,1590TA 5.2% 374.0 / 6.1 126noncoding (76/93 nt)micFsRNA antisense regulator affecting ompF expression, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base T (52/67);  new base A (3/4);  total (55/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

AATAACTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAATTATTTTTGTTGG  >  NC_000913/2313010‑2313308
                                                                                                                                                     |                                                                                                                                                      
aaTAACTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCt                                                                                                                                                        >  2:1589520/1‑150 (MQ=255)
  tAACTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCg                                                                                                                                                      <  1:706062/150‑1 (MQ=255)
   aaCTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGc                                                                                                                                                     <  2:1496585/150‑1 (MQ=255)
    aCTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCa                                                                                                                                                    <  2:1663203/150‑1 (MQ=255)
     cTTTTTCTTAATTATTTGACATCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCAt                                                                                                                                                   <  2:602300/150‑1 (MQ=255)
     cTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTGACACATATTTCAACCGGATGCCTCGCAt                                                                                                                                                   >  1:1119405/1‑150 (MQ=255)
      ttATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCAtt                                                                                                                                                  <  1:561213/150‑1 (MQ=255)
         ttCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCgg                                                                                                                                               <  2:604415/150‑1 (MQ=255)
          tCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGt                                                                                                                                             <  2:1427472/150‑1 (MQ=255)
          tCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGAATGCGAGGCATCCGGt                                                                                                                                             <  1:1290759/150‑1 (MQ=255)
          tCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTAACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGAATGCGAGGCATCCGGt                                                                                                                                             <  1:627517/150‑1 (MQ=255)
           cTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGtt                                                                                                                                            >  2:1487605/1‑150 (MQ=255)
           cTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGtt                                                                                                                                            >  2:830120/1‑150 (MQ=255)
           cTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGtt                                                                                                                                            <  2:517964/150‑1 (MQ=255)
           cTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGAAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGAATGCGAGGCATCCGGtt                                                                                                                                            <  1:5370/150‑1 (MQ=255)
            ttAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTAAACCGGATGACTCGCATTCGGttt                                                                                                                                           >  1:356467/1‑150 (MQ=255)
                gTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTAGCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACAGGATGCCTCTCATTCGGtattttt                                                                                                                                       >  1:1498621/1‑150 (MQ=255)
                 tATTTGACAGCACTGAATGTCAACACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGtttttttt                                                                                                                                      >  1:253798/1‑150 (MQ=255)
                  aTTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTa                                                                                                                                     <  1:131708/150‑1 (MQ=255)
                    ttGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTAcc                                                                                                                                   <  1:1484451/150‑1 (MQ=255)
                      taCAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCTCATTCGGTTTTTTTTACCct                                                                                                                                 <  1:451727/149‑1 (MQ=255)
                        cAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATATATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCcttc                                                                                                                               >  1:71930/1‑150 (MQ=255)
                          gCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTACCCTTCttt                                                                                                                            <  1:495042/150‑1 (MQ=255)
                           cACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCttt                                                                                                                            >  1:1180492/1‑150 (MQ=255)
                             cTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTAcc                                                                                                                                   >  1:1539276/1‑141 (MQ=255)
                             cTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTAcc                                                                                                                                   <  2:1539276/141‑1 (MQ=255)
                             cTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTac                                                                                                                          <  1:651908/150‑1 (MQ=255)
                             cTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTAATTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTac                                                                                                                          <  1:1035711/150‑1 (MQ=255)
                               gAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTacac                                                                                                                        <  1:1705685/150‑1 (MQ=255)
                                aaTGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTacaca                                                                                                                       <  2:871567/150‑1 (MQ=255)
                                   gTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACtt                                                                                                                    >  1:890770/1‑150 (MQ=255)
                                    tCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACttt                                                                                                                   <  2:541658/150‑1 (MQ=255)
                                     caaaacaaaaCCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACtttt                                                                                                                  >  1:1060664/1‑150 (MQ=255)
                                     caaaacaaaaCCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACtgtt                                                                                                                  >  2:1341210/1‑150 (MQ=255)
                                       aaacaaaacCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCa                                                                                                                <  1:1692883/150‑1 (MQ=255)
                                        aacaaaacCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCat                                                                                                               <  1:1138019/150‑1 (MQ=255)
                                         acaaaacCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGAATGCGAGGCATCCGGttg                                                                                                                                           <  2:254545/121‑2 (MQ=255)
                                         acaaaacCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGGTTACCCCTATTTCAACCGAATGCGAGGCATCCGGttg                                                                                                                                           >  1:254545/1‑120 (MQ=255)
                                         acaaaaaCTTCACTCTCAACTAGAATAACTCCCGCTTTCATCATTAACTTTATTTCTTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCatt                                                                                                              <  2:1269596/150‑1 (MQ=255)
                                          caaaacCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCatta                                                                                                             >  1:1423494/1‑150 (MQ=255)
                                              acCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTTACCCTTCTTTACACACTTTTCATTATTc                                                                                                          >  2:349270/1‑150 (MQ=255)
                                              acCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCt                                                                                                         <  2:1415872/150‑1 (MQ=255)
                                              acCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCt                                                                                                         <  2:178121/150‑1 (MQ=255)
                                               ccTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCtg                                                                                                        >  2:1024486/1‑150 (MQ=255)
                                                 ttCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCtgtg                                                                                                      <  1:1643806/150‑1 (MQ=255)
                                                  tCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGc                                                                                                     <  2:142886/150‑1 (MQ=255)
                                                    aCTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTa                                                                                                   <  2:978361/150‑1 (MQ=255)
                                                     cTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTAc                                                                                                  >  2:1040942/1‑150 (MQ=255)
                                                     cTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTAc                                                                                                  >  1:213414/1‑150 (MQ=255)
                                                         cAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACcaca                                                                                              >  2:1526019/1‑150 (MQ=255)
                                                           aCTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGa                                                                                            >  1:1243757/1‑150 (MQ=255)
                                                             tAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCACGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTAACACAGaaa                                                                                          >  2:116338/1‑150 (MQ=255)
                                                                 aTAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACt                                                                                      <  2:1558436/150‑1 (MQ=255)
                                                                  tAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGTATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACta                                                                                     <  1:49107/150‑1 (MQ=255)
                                                                  tAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACta                                                                                     >  2:44139/1‑150 (MQ=255)
                                                                        ccGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGc                                                                               <  2:1183442/150‑1 (MQ=255)
                                                                         tgCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCt                                                                              <  2:223724/149‑1 (MQ=255)
                                                                         cGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCt                                                                              <  2:1657724/150‑1 (MQ=255)
                                                                          gCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACTCTCTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGAtt                                                                             >  2:824600/1‑150 (MQ=255)
                                                                           cTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTg                                                                            <  2:459233/150‑1 (MQ=255)
                                                                              tcatcaTTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTa                                                                         <  2:1008984/150‑1 (MQ=255)
                                                                              tcatcaTTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTa                                                                         <  2:138932/150‑1 (MQ=255)
                                                                                atcatTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAAc                                                                       <  1:92507/150‑1 (MQ=255)
                                                                                 tcatTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACt                                                                      <  2:101906/150‑1 (MQ=255)
                                                                                  catTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTa                                                                     >  2:902036/1‑150 (MQ=255)
                                                                                     tAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTAttt                                                                  >  1:365883/1‑150 (MQ=255)
                                                                                      aaCTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTc                                                                 <  1:1717805/150‑1 (MQ=255)
                                                                                        cTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTca                                                                >  1:762479/1‑150 (MQ=255)
                                                                                        cTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTcac                                                               <  2:1142525/150‑1 (MQ=255)
                                                                                              tttttaCCTTAATTAATTTTTTAATGATTGTTTACCCATATTTCAACGGTATGCCTCGCATTGGTTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAaataa                                                         <  1:1589520/147‑1 (MQ=255)
                                                                                               tattaCCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGTTTTTTTTTAACCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAAAGATTGTTAACTATTTCACAaataat                                                        >  1:968065/1‑150 (MQ=255)
                                                                                                   aCCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAAc                                                    <  1:1620148/150‑1 (MQ=255)
                                                                                                        cattcattTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCg                                               >  1:1496788/1‑150 (MQ=255)
                                                                                                        cattcattTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCg                                               <  2:1681534/150‑1 (MQ=255)
                                                                                                             attTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATaa                                          <  2:71930/150‑1 (MQ=255)
                                                                                                                tCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAAttt                                       >  2:410227/1‑150 (MQ=255)
                                                                                                                 cTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTc                                      >  2:1701809/1‑150 (MQ=255)
                                                                                                                  tGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTATTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTcc                                     <  1:830120/150‑1 (MQ=255)
                                                                                                                    aaTGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAg                                   <  1:351978/150‑1 (MQ=255)
                                                                                                                     aTGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGc                                  >  1:382751/1‑150 (MQ=255)
                                                                                                                       gTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCaa                                >  1:1491834/1‑150 (MQ=255)
                                                                                                                         cTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATc                              >  1:1326552/1‑150 (MQ=255)
                                                                                                                         cTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATc                              >  1:1333717/1‑150 (MQ=255)
                                                                                                                          tGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCt                             <  2:1243757/150‑1 (MQ=255)
                                                                                                                            tttACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCttt                           >  1:756712/1‑150 (MQ=255)
                                                                                                                             ttACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTg                          <  1:44139/150‑1 (MQ=255)
                                                                                                                               aCCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGt                         <  2:1244681/150‑1 (MQ=255)
                                                                                                                                ccccTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGtt                        >  2:177007/1‑150 (MQ=255)
                                                                                                                                  catATTTCAAAAGTATTCCTCGCATTCGGTTTTTTTTACACATCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTAt                     <  1:269688/148‑1 (MQ=255)
                                                                                                                                   cTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTAtt                    <  1:1383522/150‑1 (MQ=255)
                                                                                                                                    tttttCAAACGTATGCCTCGCATTCGTTTTTTTTTACCCTTCATTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTAttt                   <  2:320805/148‑1 (MQ=255)
                                                                                                                                        aaaaCCGAATGCGAGGCATCCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCaa               >  2:1644620/3‑150 (MQ=255)
                                                                                                                                         cAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAat              >  1:1101333/1‑150 (MQ=255)
                                                                                                                                         cAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTAATTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAat              >  2:961557/1‑150 (MQ=255)
                                                                                                                                            ccGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAattat           >  2:712094/1‑150 (MQ=255)
                                                                                                                                              ggATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAATTAttt         <  2:1087954/150‑1 (MQ=255)
                                                                                                                                               gATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAATTAtttt        <  2:94290/150‑1 (MQ=255)
                                                                                                                                                  gCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAATTATTTttgt     >  2:685194/1‑150 (MQ=255)
                                                                                                                                                    cTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAATTATTTttgttg   <  1:1460216/150‑1 (MQ=255)
                                                                                                                                                     tCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAATTATTTTTGTTgg  >  1:594867/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AATAACTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAATTATTTTTGTTGG  >  NC_000913/2313010‑2313308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: