Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,316,905 T→C 6.3% intergenic (+78/+122) rcsB → / ← rcsC response regulator in two‑component regulatory system with RcsC and YojN/hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,316,9050TC 6.3% 381.0 / 11.1 127intergenic (+78/+122)rcsB/rcsCresponse regulator in two‑component regulatory system with RcsC and YojN/hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base T (73/46);  new base C (4/4);  total (77/50)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

Only 100 of 128 total aligned reads displayed.

GTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTC  >  NC_000913/2316759‑2317054
                                                                                                                                                  |                                                                                                                                                     
gtCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                    >  2:562417/1‑150 (MQ=255)
gtCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                                   <  1:201352/150‑1 (MQ=255)
  cGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                                  >  1:550990/1‑150 (MQ=255)
  cGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGAATGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                                  >  2:447876/1‑150 (MQ=255)
   gagaACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                                 >  2:919129/1‑150 (MQ=255)
    agaACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTTAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                                <  1:1639681/150‑1 (MQ=38)
    agaACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                                >  2:643589/1‑150 (MQ=255)
    agaACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                                <  1:323234/150‑1 (MQ=38)
      aaCGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                                                                                              >  2:616424/1‑150 (MQ=255)
      aaCGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATAAGGCGTTTACGCCGCATCCGGcaa                                                                                                                                              <  2:146991/150‑3 (MQ=255)
       aCGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCTGGCAGATAAAGAGTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATAAGGCGTTTACGCCGCATCCGGcaat                                                                                                                                             <  1:518765/150‑4 (MQ=255)
        cGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                                            <  2:785917/150‑1 (MQ=255)
        cGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGAGTAAACGCCTTATCCGGCCTAc                                                                                                                                            >  1:95355/1‑150 (MQ=255)
          atatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGa                                                                                                                                          >  2:708949/1‑150 (MQ=255)
          atatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGa                                                                                                                                          >  2:242532/1‑150 (MQ=255)
           tatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGAt                                                                                                                                         <  2:499568/150‑1 (MQ=255)
             acGCCCTTCTGAATTATCTCTCTTCTGTGAACTTAAGTCCTGGAGATATAGTCTATTCACGTGTAGGCCAGATTTTACGCGTTAGTGTCTTAGCTGGCATTTGCACCGATAGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTc                                                                                                                                       <  1:238954/149‑1 (MQ=25)
              cGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTcc                                                                                                                                      >  2:1057063/1‑150 (MQ=255)
               gCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTccc                                                                                                                                     >  1:750077/1‑150 (MQ=255)
                cccTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCa                                                                                                                                    <  2:531009/150‑1 (MQ=255)
                  ctgctgAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCatt                                                                                                                                  <  2:726058/150‑1 (MQ=255)
                    gctgAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCattat                                                                                                                                <  1:598613/150‑1 (MQ=255)
                      tgAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTAttt                                                                                                                              <  2:379595/150‑1 (MQ=255)
                        aaTTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTca                                                                                                                            >  2:172747/1‑150 (MQ=255)
                           tATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTcaaca                                                                                                                         >  2:1470735/1‑150 (MQ=255)
                            aTCTCTCTTCAGTGACATTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATATcaacaa                                                                                                                        >  1:870514/1‑150 (MQ=255)
                                 tctTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTAc                                                                                                                   >  1:1043950/1‑150 (MQ=255)
                                      aGTGACCTTTAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTaa                                                                                                              <  2:296083/150‑1 (MQ=255)
                                      aGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTaa                                                                                                              >  2:65554/1‑150 (MQ=255)
                                      aGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTaa                                                                                                              >  1:433219/1‑150 (MQ=255)
                                       gTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTaaa                                                                                                             >  1:1697647/1‑150 (MQ=255)
                                         gACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGt                                                                                                           >  1:1689823/1‑150 (MQ=255)
                                            cTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAgg                                                                                                        <  1:1276355/150‑1 (MQ=255)
                                             ttAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGc                                                                                                       >  2:1552305/1‑150 (MQ=255)
                                               aaGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCa                                                                                                     <  2:429672/150‑1 (MQ=255)
                                                 gTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGa                                                                                                   >  1:384957/1‑150 (MQ=255)
                                                 gTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGa                                                                                                   >  2:62814/1‑150 (MQ=255)
                                                     ggCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAg                                                                                               <  1:170624/150‑1 (MQ=255)
                                                          aTAACGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCAGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGAcgcg                                                                                          <  2:858994/150‑1 (MQ=255)
                                                          aTAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGAcgcg                                                                                          <  1:1068133/150‑1 (MQ=255)
                                                            aaaGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTc                                                                                        <  2:1431606/150‑1 (MQ=255)
                                                            aaaGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTc                                                                                        >  2:750370/1‑150 (MQ=255)
                                                                aCTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCg                                                                                    >  1:1594907/1‑150 (MQ=255)
                                                                    aTCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGc                                                                                >  1:15484/1‑150 (MQ=255)
                                                                      cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCAt                                                                              >  1:1159632/1‑150 (MQ=255)
                                                                        ccTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCt                                                                            >  2:511700/1‑150 (MQ=255)
                                                                          tGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTgg                                                                          <  1:1151386/150‑1 (MQ=255)
                                                                             aGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCAt                                                                       >  2:1282848/1‑150 (MQ=255)
                                                                              ggCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCAtt                                                                      >  1:482995/1‑150 (MQ=255)
                                                                                ccAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTg                                                                    >  2:1437714/1‑150 (MQ=255)
                                                                                ccAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTg                                                                    >  1:1464224/1‑150 (MQ=255)
                                                                                ccAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTg                                                                    <  2:1527306/150‑1 (MQ=255)
                                                                                ccAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTg                                                                    >  1:1105731/1‑150 (MQ=255)
                                                                                ccAGATAAGACACTAACGCGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTg                                                                    >  1:1599852/1‑150 (MQ=38)
                                                                                 cAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGACGCATCTGGCATTTga                                                                   >  1:691430/1‑149 (MQ=255)
                                                                                 cAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATAAATTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGAGTCCCATCTGGCAATTGc                                                                   >  1:321612/1‑150 (MQ=37)
                                                                                  aGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTTGCATTTGCa                                                                  >  1:622752/1‑150 (MQ=255)
                                                                                  aGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCACATTATTTCAACAAATTACATTAAAGTAGGCAAGATAAGACGCGTCAGCGACGCATCTTGCATTTgaa                                                                  >  1:461884/1‑148 (MQ=255)
                                                                                   gATAAGACGCGTTAGTGTTTTATCTGGCATTTGCACCTATTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCAc                                                                 <  2:273481/150‑1 (MQ=255)
                                                                                   gATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCAc                                                                 >  1:382221/1‑150 (MQ=255)
                                                                                     tAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCCGGCAttcagtgca                                                               <  1:1065362/150‑8 (MQ=255)
                                                                                      aaGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGa                                                              >  2:1585294/1‑150 (MQ=255)
                                                                                          cgcgTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGc                                                          >  2:1109338/1‑150 (MQ=255)
                                                                                          cgcgTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGc                                                          >  1:1211271/1‑150 (MQ=255)
                                                                                                aGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATg                                                    >  1:881089/1‑150 (MQ=255)
                                                                                                 gtgtCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATACGGCCTACGATTCCCATTATATCAACAAATTAAATTAAAGTAGTCCAGCTAACACGAGTCAGCGTCGCATCTGTCATTTGCACTAAATGCCGCAtac                                                   >  1:148756/1‑148 (MQ=37)
                                                                                                   gtCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATgcgg                                                 <  1:511700/150‑1 (MQ=255)
                                                                                                    tCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGACAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATgcggc                                                >  1:543943/1‑150 (MQ=255)
                                                                                                      ttATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGt                                              <  1:1200120/150‑1 (MQ=255)
                                                                                                        aTCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTaa                                            <  2:1688279/150‑1 (MQ=255)
                                                                                                          cTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAAc                                          >  1:766490/1‑150 (MQ=255)
                                                                                                          cTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAAATACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTAGCATCTGGCATTTGAACTGAATGCCGGATGCGGCGTAAAc                                          >  1:223578/1‑150 (MQ=255)
                                                                                                          cTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTACAACAAATTACATTAAAGAAGGCCAGATAACACGCTTCAGCGTCGCATCTGCCATTTGCACAGAATTACGGATGCGGCGTaaaa                                          >  1:875485/1‑149 (MQ=37)
                                                                                                               aTTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCtt                                     <  2:1043950/150‑1 (MQ=255)
                                                                                                               aTTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACACCtt                                     <  2:1689823/150‑1 (MQ=255)
                                                                                                                    cACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCg                                <  1:1379323/150‑1 (MQ=255)
                                                                                                                    cACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCg                                >  1:757095/1‑150 (MQ=255)
                                                                                                                    cACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCg                                <  1:1137441/150‑1 (MQ=255)
                                                                                                                       cGATTGCCGGATGAGGCTTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTcc                             <  1:627885/150‑1 (MQ=255)
                                                                                                                        gATTGCCTGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCt                            <  2:101303/150‑1 (MQ=255)
                                                                                                                          ttGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATAAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTAc                          <  1:1351118/150‑1 (MQ=255)
                                                                                                                          aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTAc                          >  2:775666/3‑150 (MQ=25)
                                                                                                                           ggCCGGATAAGGCGTTTACGCAGCATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACg                         >  1:965199/2‑150 (MQ=25)
                                                                                                                            gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGa                        <  2:1211271/150‑1 (MQ=255)
                                                                                                                               ggataaggcgTTTACGCCGCATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATc                     >  2:703938/7‑150 (MQ=25)
                                                                                                                               ggataaggcgTTTACGCCGCATCCGGCCTACGATTCCCAATATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAACCGCCTTATCCGTCCTACGAATc                     >  2:267558/7‑150 (MQ=38)
                                                                                                                                  tGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCcg                  <  1:242532/150‑1 (MQ=255)
                                                                                                                                   gcggcgTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCcgc                 >  1:1339684/1‑150 (MQ=255)
                                                                                                                                   gcggcgTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCcgc                 <  2:908777/150‑1 (MQ=255)
                                                                                                                                    cggcgTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCcgcg                >  1:1571211/1‑150 (MQ=255)
                                                                                                                                    cggcgTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCcgcg                <  2:1699839/150‑1 (MQ=255)
                                                                                                                                      gcgTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCCACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGAt              >  2:957435/1‑150 (MQ=255)
                                                                                                                                      gcgTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCTTTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGAt              >  1:1123282/1‑150 (MQ=255)
                                                                                                                                      gcgTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGAt              >  2:1298933/1‑150 (MQ=255)
                                                                                                                                       cgTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGAtt             >  1:1226493/1‑150 (MQ=255)
                                                                                                                                          aaaCGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTcc          >  2:555928/1‑150 (MQ=255)
                                                                                                                                          aaaCGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTcc          >  2:111971/1‑150 (MQ=255)
                                                                                                                                            aCGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTg        >  1:959834/1‑150 (MQ=255)
                                                                                                                                              gCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGAc      <  2:299572/150‑1 (MQ=255)
                                                                                                                                                  tATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCctc  >  2:252786/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTACATTAAAGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTC  >  NC_000913/2316759‑2317054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: