Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,319,586 C→T 13.2% A97A (GCG→GCA rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,319,5860CT13.2% 217.4 / 18.2 83A97A (GCG→GCArcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (42/30);  new base T (8/3);  total (50/33)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01

AAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAA  >  NC_000913/2319438‑2319734
                                                                                                                                                    |                                                                                                                                                    
aaaaTACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCg                                                                                                                                                     >  2:962736/1‑150 (MQ=255)
aaaaTACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTAAAACGCAGGCACATCCg                                                                                                                                                     >  2:87885/1‑150 (MQ=255)
      cccGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCGGATGTGCCTGCg                                                                                                                                               <  1:743963/150‑1 (MQ=255)
       ccGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCGTAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCGGATGTGCCTGCGt                                                                                                                                              <  2:961942/150‑1 (MQ=255)
          gTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTtc                                                                                                                                           <  2:1579901/150‑1 (MQ=255)
           tttAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTtct                                                                                                                                          >  1:1044767/1‑150 (MQ=255)
             tAGATCTTAAGACGCATAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCATTTACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCg                                                                                                                                        <  1:1362200/150‑1 (MQ=255)
             tAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCg                                                                                                                                        <  1:1730000/150‑1 (MQ=255)
              aGATCGTACGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGt                                                                                                                                       <  1:1253336/150‑1 (MQ=255)
              aGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCGGATGTGCCTGCGtttgaacc                                                                                                                                       <  1:1609972/150‑6 (MQ=255)
              aGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACATCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCGGGGACATCCGCCTGCGTGTCTCGt                                                                                                                                       >  1:205323/1‑150 (MQ=255)
                   gTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCAcg                                                                                                                                  >  2:902562/1‑150 (MQ=255)
                         cGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAGCCTCGCCAGGTGTTACTCATAGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATACGCCTGCGTTTCTCGTCCACGCGGGGa                                                                                                                            >  1:1534403/1‑150 (MQ=255)
                          gCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCCCGCGGGGaa                                                                                                                           >  1:955862/1‑150 (MQ=255)
                           cAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGACCAATCGGAGTCGGCACACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGacg                                                                                                                          >  1:208515/1‑148 (MQ=255)
                            aGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAg                                                                                                                         >  1:411207/1‑150 (MQ=255)
                                 aaTTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCacac                                                                                                                    <  1:585620/150‑1 (MQ=255)
                                    tATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGt                                                                                                                 >  1:1728964/1‑150 (MQ=255)
                                        gcgcCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTc                                                                                                             >  1:1116591/1‑150 (MQ=255)
                                         cgcCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACTCGGGGAAAGCAAACCGTTTTCt                                                                                                            >  1:142584/1‑150 (MQ=255)
                                          gcCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTg                                                                                                           >  2:543447/1‑150 (MQ=255)
                                             aGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGAGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCCCATCCGCCTGCGTTTCTCGTCCCCGCGGGGAAAGCACACCGTTTTCTGCCg                                                                                                        >  2:1101816/1‑150 (MQ=255)
                                                       aaaCCACGCCAATGCCTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGACTGCGGTTCTAGTCCACGAGGGGAAAGCACCCCGTTTTATGCAGATAAGCGAtt                                                                                              >  2:1305427/1‑150 (MQ=255)
                                                       aaaCCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGAtt                                                                                              >  2:657612/1‑150 (MQ=255)
                                                        aaCCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGAttt                                                                                             >  2:203841/1‑150 (MQ=255)
                                                        aaCCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGAGAttt                                                                                             >  1:1439519/1‑150 (MQ=255)
                                                            aCGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCgg                                                                                         >  1:1371730/1‑150 (MQ=255)
                                                             cGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCCCACCGTTTTCTGCCGATAAGCGATTTTCGGc                                                                                        >  1:1318666/1‑150 (MQ=255)
                                                                 aaTGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGAAAATCGCTTATCGGcagaa                                                                                    <  2:122995/150‑5 (MQ=255)
                                                                   tGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTa                                                                                  <  1:936998/150‑1 (MQ=255)
                                                                     aCTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACt                                                                                >  2:1702488/1‑150 (MQ=255)
                                                                         cAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGAAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAg                                                                            >  1:392625/1‑150 (MQ=255)
                                                                          agagAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGc                                                                           >  2:670649/1‑150 (MQ=255)
                                                                           gagaACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGct                                                                          <  2:1559812/150‑1 (MQ=255)
                                                                              aaCCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCtt                                                                       <  2:174321/150‑1 (MQ=255)
                                                                               aCCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGGTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCttt                                                                      >  1:1273927/1‑150 (MQ=255)
                                                                                 cTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGGTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTtca                                                                    <  1:1215851/150‑1 (MQ=255)
                                                                                   cGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGAGATTTTCGGCGATGTACTTCATCTCTTtcatc                                                                  >  2:1265901/1‑150 (MQ=255)
                                                                                     ccAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTcatcac                                                                <  2:1264347/150‑1 (MQ=255)
                                                                                          tgtTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCag                                                           <  2:191506/150‑1 (MQ=255)
                                                                                             tACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGTCGATGTACTTCAGCTCTTTCATCACATCAGAgg                                                        <  1:1565709/150‑1 (MQ=255)
                                                                                                tCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGc                                                     <  2:1116591/150‑1 (MQ=255)
                                                                                                  aTTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCCCATCCGCCTGCGGTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGAGGTCCTTCAGCTCGTTCATCACATCAGAGGTGCgg                                                   >  2:141935/1‑149 (MQ=255)
                                                                                                    tGCGGAACAATCGGAGTCGGCAAACCGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTg                                                 <  2:840721/150‑1 (MQ=255)
                                                                                                    tGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTg                                                 <  2:547088/150‑1 (MQ=255)
                                                                                                    tGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTg                                                 <  1:203841/150‑1 (MQ=255)
                                                                                                     gCGGAACAATCGGTGTCGGCAATCAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGGCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGa                                                >  2:224272/1‑150 (MQ=255)
                                                                                                     gCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTgt                                                >  1:1675896/1‑149 (MQ=255)
                                                                                                     gCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGa                                                >  2:656897/1‑150 (MQ=255)
                                                                                                      cGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATCTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGaa                                               >  1:889821/1‑150 (MQ=255)
                                                                                                       ggAACAAGCGGAGGCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGGTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAAt                                              <  2:848590/150‑1 (MQ=255)
                                                                                                          aCAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATaaa                                           <  1:902562/150‑1 (MQ=255)
                                                                                                           cAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAg                                          <  2:913219/150‑1 (MQ=255)
                                                                                                            aaTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTATCGTCCACGCGGGGAAAGCACACCGTTTTATGCCGATAGGCGATTTTCGGCGATGTACTTCCGCTCTTTAATCACATCAGAGGTGCGTTGAATAACgc                                         >  1:913496/1‑150 (MQ=255)
                                                                                                             aTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGTGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCATCTCTTTCATCACATCAGAGGTGCGTTGAATAAcgcg                                        >  2:843528/1‑150 (MQ=255)
                                                                                                             aTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAgcg                                        >  2:41967/1‑150 (MQ=255)
                                                                                                               ctgAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCTTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCTTTGAATAAAGCGCt                                      <  2:1228927/148‑1 (MQ=255)
                                                                                                                ggAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTg                                     >  2:1268899/1‑150 (MQ=255)
                                                                                                                   gTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCCCGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCCGCTCTTTCATCACATCAGAGGTGCGTTGAATAACGCGCTgcgc                                  >  2:1226381/1‑150 (MQ=255)
                                                                                                                   gTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGc                                  <  2:1728964/150‑1 (MQ=255)
                                                                                                                     cGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCt                                >  1:1412322/1‑150 (MQ=255)
                                                                                                                      ggCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTg                               >  1:943473/1‑150 (MQ=255)
                                                                                                                       gCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGa                              >  1:769230/1‑150 (MQ=255)
                                                                                                                            cAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACCCCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCGTTCATCACATCAGAGGTGCGTTGAAGAAAGCGCTGAGCCTGAGCGGa                         >  1:476525/1‑150 (MQ=255)
                                                                                                                            cAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGa                         <  1:543447/150‑1 (MQ=255)
                                                                                                                                    gaaaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGAt                 >  2:393332/2‑150 (MQ=255)
                                                                                                                                    gaaaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGAt                 >  1:566756/2‑150 (MQ=255)
                                                                                                                                    gaaaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTACCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGAt                 >  2:474828/2‑150 (MQ=255)
                                                                                                                                    cAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGAt                 <  1:962736/150‑1 (MQ=255)
                                                                                                                                     aaaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTGTCTGCGGATGAGCGCTGTTCGGCGATGTACGTCAGATATTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGACATCAGAtt                >  1:281383/1‑150 (MQ=255)
                                                                                                                                     aaaCGCAGGCACATCCGCGTGAGTTTCTCGTCTACGAGGGGATAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTTCTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGAtt                <  1:166227/150‑1 (MQ=255)
                                                                                                                                     aaaCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCTATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGAtt                <  1:1265901/150‑1 (MQ=255)
                                                                                                                                      aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCCCCCCGTTTACTGCCGATAAGCGATTTTCGGCGATGTACTTCAGATGTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa               >  2:437226/1‑150 (MQ=255)
                                                                                                                                      aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa               >  1:736563/1‑150 (MQ=255)
                                                                                                                                      aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTATTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa               >  2:1127858/1‑150 (MQ=255)
                                                                                                                                      aaCGCAGGCGGATGTGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACAGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa               >  1:376690/1‑150 (MQ=255)
                                                                                                                                      aaCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa               <  1:22032/150‑1 (MQ=255)
                                                                                                                                      aaCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTa               >  1:1215456/1‑150 (MQ=255)
                                                                                                                                      aaCGCAGCCACATCCGCCTGCGTTTCTCGTCCACGCCGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCTATGTACTTCAGCTCTTTCATCACATCACAGGTGCGTTGAATAAAGCTCTGAGCCTGATCGGAACTCAGAtaa               >  2:1619305/1‑148 (MQ=255)
                                                                                                                                         gCAGGCAAATCCGCCTGCGTTTCTCGTCCACGCGGTGAAAGCACACCGTTTTCTGCCGATAAGCTATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAt            <  2:1534403/150‑1 (MQ=255)
                                                                                                                                          cAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAtt           <  1:656897/150‑1 (MQ=255)
                                                                                                                                                caTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGAc     >  2:438425/1‑150 (MQ=255)
                                                                                                                                                 aTCCGCATGCGTTTATCGTGCACGAGGGGAAAGCACACCTTTTTCTGCCTATAAGCGTTTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACg    <  2:858981/150‑1 (MQ=255)
                                                                                                                                                   ccGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCCGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTACTGACGaa  >  1:1261597/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAA  >  NC_000913/2319438‑2319734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: