Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,356,734 G→C 11.0% intergenic (+23/‑170) glpC → / → yfaD anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,356,7340GC11.0% 265.2 / 19.8 90intergenic (+23/‑170)glpC/yfaDanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base G (43/36);  new base C (5/5);  total (48/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

GAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATA  >  NC_000913/2356592‑2356882
                                                                                                                                              |                                                                                                                                                    
gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAATTGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                               >  1:1256968/1‑150 (MQ=255)
gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                               >  1:1507317/1‑150 (MQ=255)
gaagaaAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCAAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTgg                                                                                                                                               >  2:266707/1‑150 (MQ=255)
     atgcggcgCTGATCTGGTGGTGACCGACTGCGAAACCTGTAAATGGCTGATTGAGATTTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCGCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTAcc                                                                                                                                          <  1:1727693/148‑1 (MQ=255)
       gcggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCgg                                                                                                                                        <  1:1610704/150‑1 (MQ=255)
        cggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg                                                                                                                                       <  2:1724659/150‑1 (MQ=255)
         ggcgCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGc                                                                                                                                      <  2:1438497/150‑1 (MQ=255)
           cgcAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAt                                                                                                                                    >  1:884533/1‑150 (MQ=255)
             cAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAttt                                                                                                                                  >  2:1546921/1‑150 (MQ=255)
              aGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtttt                                                                                                                                 <  2:1721276/150‑1 (MQ=255)
                  cTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg                                                                                                                             <  1:127888/150‑1 (MQ=255)
                  cTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg                                                                                                                             <  2:1688897/150‑1 (MQ=255)
                   tggtggTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGCAt                                                                                                                                    <  1:213367/142‑1 (MQ=255)
                   tggtggTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGCAt                                                                                                                                    >  2:213367/1‑142 (MQ=255)
                   tggtggTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGCAt                                                                                                                                    >  2:641503/1‑142 (MQ=255)
                   tggtggTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCGGGCAt                                                                                                                                    <  1:641503/142‑1 (MQ=255)
                       ggtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTacac                                                                                                                        >  1:161194/1‑150 (MQ=255)
                       ggtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTacac                                                                                                                        >  1:496839/1‑150 (MQ=255)
                            ccGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTc                                                                                                                   <  2:232206/150‑1 (MQ=255)
                            ccGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTc                                                                                                                   <  2:1420846/150‑1 (MQ=255)
                                    gAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCa                                                                                                           <  2:648580/150‑1 (MQ=255)
                                       aCCTGTAAAAGGCAGCTTGAGATGTCCTCAAGGCTTCGCAGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATg                                                                                                        <  2:338398/150‑1 (MQ=255)
                                         cTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTa                                                                                                      >  1:1542515/1‑150 (MQ=255)
                                          tGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTCGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTAt                                                                                                     >  2:1313084/1‑150 (MQ=255)
                                           gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATg                                                                                                    <  2:884533/150‑1 (MQ=255)
                                           gTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAAAGTATg                                                                                                    >  2:124418/1‑150 (MQ=255)
                                              aaTGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGt                                                                                                 <  2:1539798/150‑1 (MQ=255)
                                                tGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTg                                                                                               <  1:21734/150‑1 (MQ=255)
                                                 ggCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGc                                                                                              <  1:930084/150‑1 (MQ=255)
                                                 ggCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGCCATATTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGc                                                                                              >  1:72134/1‑150 (MQ=255)
                                                    aGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAAc                                                                                           >  1:1114712/1‑150 (MQ=255)
                                                     gATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACg                                                                                          >  1:362102/1‑150 (MQ=255)
                                                      aTTGAGATGTCAACAAGTCTTCGCTGCGAACATACGATTACTCTACTGGCCCAGGCGCTGCCTTAAACTCCTTTCTGATGCCCGGTAAGCTTGTGTTTACCGGGCATTTTTGATAACACGATTCCGTGCCCAATTTATTCGTTGCAActc                                                                                         >  2:850791/1‑148 (MQ=255)
                                                       ttGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCa                                                                                        >  1:590623/1‑150 (MQ=255)
                                                          agaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTAGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTg                                                                                     >  1:292748/1‑150 (MQ=255)
                                                           gaaggccACAAGGCTCAGCGCCGACCATCCGATCCCGCTACGGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGa                                                                                    <  2:98108/145‑1 (MQ=255)
                                                           gaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGa                                                                                    >  1:1149255/1‑150 (MQ=255)
                                                           gaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGa                                                                                    >  1:1726170/1‑150 (MQ=255)
                                                           gaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGa                                                                                    >  1:1316303/1‑150 (MQ=255)
                                                           gaTGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGa                                                                                    >  1:1243998/1‑150 (MQ=255)
                                                             tGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGaaa                                                                                  >  1:1584730/1‑150 (MQ=255)
                                                             tGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGAGTCCGTGCCCAATGTATGCGTTGCAACGCAGTTaaa                                                                                  >  2:329976/1‑150 (MQ=255)
                                                                ccACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAtt                                                                               >  2:1316865/1‑150 (MQ=255)
                                                                 cacaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTc                                                                              <  1:422159/150‑1 (MQ=255)
                                                                 cacaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTc                                                                              <  2:274191/150‑1 (MQ=255)
                                                                 cacaAGTCTTCGCTGCGAACATCCGATTACGCGACTGGCCCAGGCGCTGGCTTAAACTCCTGTCTGATGCCCGGTAAGCATGTGGTTACCGGGAATTTTTGCGTACACGATTCCGTGGCGAAGGTATGCGTTGCAACGCAGTGATAATTc                                                                              >  2:67673/1‑150 (MQ=255)
                                                                 aacaAGTCTTCGCTGCGAAAATCCGATTACGCTACTGGCCCAGGCTCTGGCTTAACCTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATATTTGCGTACACGGATCCGTGCCCACTGTATGCGTTGCAACCCAGTGAAAATTc                                                                              >  1:885344/2‑150 (MQ=255)
                                                                  acaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTcc                                                                             <  2:107480/150‑1 (MQ=255)
                                                                   caAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCct                                                                            >  2:490514/1‑150 (MQ=255)
                                                                     aGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctct                                                                          <  1:394102/150‑1 (MQ=255)
                                                                     aGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctct                                                                          <  2:430760/150‑1 (MQ=255)
                                                                     aGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctct                                                                          <  1:1447154/150‑1 (MQ=255)
                                                                      ttCTTCGCTGCGAACATCCCATTACGCTACTGTCCCTGGGGCTGGCTTAAACTCCTTTCTGAAGCCCGGTAAGCATGTGGTTACCGGGCATTTTTCCGTACACGATACCGTGCCCAATGTATCCGAAGCAACGCAGTGAAAATTCCTCTg                                                                         <  1:455550/149‑1 (MQ=255)
                                                                       tCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGa                                                                        <  2:459304/150‑1 (MQ=255)
                                                                       tCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGa                                                                        <  2:1304088/150‑1 (MQ=255)
                                                                        cTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGaa                                                                       >  1:1643040/1‑150 (MQ=255)
                                                                         ttCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGaaa                                                                      <  1:1104709/150‑1 (MQ=255)
                                                                           cGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAAc                                                                    >  2:47450/1‑150 (MQ=255)
                                                                                     aTCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCACCGCAGTGAAAATTCCTCTGAAAACGTCTCGCaaa                                                          >  1:1154449/1‑150 (MQ=255)
                                                                                               gCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACt                                                <  1:1334826/150‑1 (MQ=255)
                                                                                                 tACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCACAGGCTGAAACTgg                                              <  1:467949/150‑1 (MQ=255)
                                                                                                 tACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATGCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCATAGGCTGAAACTgg                                              <  1:474427/150‑1 (MQ=255)
                                                                                                  aCTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGc                                             <  1:580229/150‑1 (MQ=255)
                                                                                                   cTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCa                                            >  1:1428083/1‑150 (MQ=255)
                                                                                                     ggCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTACGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCGGAAACTGGCAGa                                          <  2:1054901/150‑1 (MQ=255)
                                                                                                     ggCCCAGGCGCGGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGa                                          >  2:771995/1‑150 (MQ=255)
                                                                                                      gCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGAGTTGCATCGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGAt                                         >  1:960565/1‑150 (MQ=255)
                                                                                                      gCCCAGGCGCTGGCTTAAACTCCATTCTGAAGCCCGGTAAGGATGTTGTTAACGGGCATTTTTGCGTACACGTTTCCGTGCCCAATGTTTGCGTTGCAACGCAGTGTAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGAt                                         <  1:266707/150‑1 (MQ=255)
                                                                                                        cctggCGCTGGCTTTAACTACTTTCTGATGCCCGGTAAGCATGTGGTTTCCGGGAATTTTTGCTTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGt                                       <  2:1435866/147‑1 (MQ=255)
                                                                                                            gcgcTGGCTTAAACTCCTTTCTGATGTCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGGCTCGCAAAGGCTGAAACTGGCAGATGTCaaa                                   >  2:220999/1‑150 (MQ=255)
                                                                                                             cgcTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAg                                  <  2:1449985/150‑1 (MQ=255)
                                                                                                              gcTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAgg                                 <  1:1727013/150‑1 (MQ=255)
                                                                                                               cTGGCTTAAACTCCTTTCAGATGCCCGGTAAGCAGGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAGTTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGc                                <  1:1313084/150‑1 (MQ=255)
                                                                                                                 ggCTTAAACTCCTTTCTGATGCCCGGTAATCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCt                              <  1:1315665/150‑1 (MQ=255)
                                                                                                                      aaaCTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGAt                         >  2:1442490/1‑150 (MQ=255)
                                                                                                                      aaaCTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGAt                         >  2:1427281/1‑150 (MQ=255)
                                                                                                                       aaCTCCTTTCTGATGCCCGGTAAGCAGGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCGATTGTATGCGTTGCAACGCTGTGAATATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATa                        <  1:894516/150‑1 (MQ=255)
                                                                                                                          tCCTTTCTGATGCCCGGTAAGCATGTGGTTACAGGGCATTTTTGCGTACACGATACCGTGCCCAATGTATGCGTTGCAACGCAGAGAAAAATCCTCTGAAAACGTCTCGCAAAGGCTGATACTGGCAGATGTAAAAGTCCTCGGATAAcc                     >  1:690029/1‑150 (MQ=255)
                                                                                                                            cTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGt                   >  2:1232185/1‑150 (MQ=255)
                                                                                                                            cTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGt                   >  2:880835/1‑150 (MQ=255)
                                                                                                                                cTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATg               >  2:673055/1‑150 (MQ=255)
                                                                                                                                 aaaTGCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGt              >  1:949050/3‑150 (MQ=255)
                                                                                                                                   aTGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAACCGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcg            <  1:1496486/150‑1 (MQ=255)
                                                                                                                                    tGCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgc           >  1:1315750/1‑150 (MQ=255)
                                                                                                                                     gCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg          >  1:489419/1‑150 (MQ=255)
                                                                                                                                     gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTcgcg          >  1:572918/1‑150 (MQ=255)
                                                                                                                                        cGGTAAGCATGTGGTGACCGTGCATTTTTGTGTACACGATTCCGTGCCCAATGTATGCGGTGCAACGCAGTGAAAATTCATCTGAAAACGTCTCGCAAAGGCCGAAACTGGCAGAGGTCAAAGGCCTGGGATAACCGTAATGTCGCGtca       <  1:1222483/150‑1 (MQ=255)
                                                                                                                                          gTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcata     >  1:581814/1‑149 (MQ=255)
                                                                                                                                           tAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGtcatca    >  1:855667/1‑150 (MQ=255)
                                                                                                                                             aGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATa  >  1:212738/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GAAGAAAGCGGCGCAGATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGTCATCATA  >  NC_000913/2356592‑2356882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: