Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,387,592 T→A 5.3% intergenic (+155/+118) yfbM → / ← yfbN DUF1877 family protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,387,5920TA 5.3% 317.1 / 3.9 104intergenic (+155/+118)yfbM/yfbNDUF1877 family protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (52/46);  new base A (3/3);  total (55/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATGGCGACACGGGA  >  NC_000913/2387443‑2387741
                                                                                                                                                     |                                                                                                                                                     
cTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATAtt                                                                                                                                                       >  2:593741/1‑150 (MQ=255)
   gATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCgg                                                                                                                                                    >  1:1682698/1‑150 (MQ=255)
       ccTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATg                                                                                                                                                <  2:362672/150‑1 (MQ=255)
       ccTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATg                                                                                                                                                <  2:409055/150‑1 (MQ=255)
        cTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGt                                                                                                                                               >  2:1394614/1‑150 (MQ=255)
        cTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGt                                                                                                                                               <  2:283811/150‑1 (MQ=255)
         tcgcgcagaAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTa                                                                                                                                              <  1:964940/144‑1 (MQ=255)
          gtgggAGAAAGACCCATAAGTTAAGATTAACGCCAGGATCATCTTGAAAAAATCTTACACCCTGGTCCCGCTTTTATCCCATTGTATAAATATTACTAACTGCTTGCTTAGTCGCATTGTTAAATGCAATACCGAATATACGGTAttgca                                                                                                                                             <  2:270538/148‑5 (MQ=255)
          gggggAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTaa                                                                                                                                             >  2:333174/1‑150 (MQ=255)
            gggAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGATTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACa                                                                                                                                           >  2:274643/1‑150 (MQ=255)
            gggAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTAAACCATGGTCCGGTTTTTATAACCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATACGCTATGTAACa                                                                                                                                           >  1:1194036/1‑150 (MQ=255)
              gagaAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCGTTGTATAATTATTACTAAATGGTTGGTTTGTCGCATGGTTAAATGCAATCCCGTTTTTTCGGTATGTAACAgg                                                                                                                                         >  2:1399331/1‑149 (MQ=255)
               agaAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCg                                                                                                                                        <  1:714181/150‑1 (MQ=255)
                gaAAGACCCATTAGTTAAGATTTCCGCCACGATCATCTTGAAAAACTCTTTACCCATGGTCCGGTTTTTATCCCCTTGTATAAATATTCCTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGa                                                                                                                                       <  2:96573/150‑1 (MQ=255)
                  aaGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGata                                                                                                                                     >  2:114198/1‑150 (MQ=255)
                  aaGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGata                                                                                                                                     <  1:1433309/150‑1 (MQ=255)
                  aaGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGata                                                                                                                                     >  1:1096501/1‑150 (MQ=255)
                  aaGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGata                                                                                                                                     >  2:741143/1‑150 (MQ=255)
                   aGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGATTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGatat                                                                                                                                    >  2:311995/1‑150 (MQ=255)
                    gACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATc                                                                                                                                   >  1:1138435/1‑150 (MQ=255)
                    gACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATc                                                                                                                                   <  1:998896/150‑1 (MQ=255)
                     aCCCATTAGTTAAGATTTACGCCAGGATCATCTTTAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATcc                                                                                                                                  <  1:119240/150‑1 (MQ=255)
                        cATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATAAATAAATCATTGATTATACAAATTTTTAAATCAAATACCTTAAATTCGTTATTTAACAACGATATcaaat                                                                                                                               >  1:996893/1‑146 (MQ=255)
                           tAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCtgtg                                                                                                                            >  2:1086149/1‑150 (MQ=255)
                            aGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGc                                                                                                                           >  1:1420798/1‑150 (MQ=255)
                               tAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTgg                                                                                                                        <  2:541177/150‑1 (MQ=255)
                                  gATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCaa                                                                                                                     >  1:1543877/1‑150 (MQ=255)
                                       aCGGCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGAATGTAACAGCGATATCCTCTGTGCTGGCAACGATc                                                                                                                <  1:724841/150‑1 (MQ=255)
                                       aCGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATc                                                                                                                <  1:1086149/150‑1 (MQ=255)
                                       aCGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATc                                                                                                                <  2:1543877/150‑1 (MQ=255)
                                        cGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCt                                                                                                               >  2:1461172/1‑150 (MQ=255)
                                         gCCAGGATCATCTTGAAAAAATCCTTAACCATGGTGCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTCAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTg                                                                                                              <  2:778307/150‑1 (MQ=255)
                                          ccAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATTCAATACCTTATATTCGGTATGTAACAGCGATATCCTCTGTGATGGAAACGATCTGt                                                                                                             >  1:976873/1‑150 (MQ=255)
                                          ccAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCATCTGTGCTGGCAACGATCTGt                                                                                                             >  2:1368947/1‑150 (MQ=255)
                                           cAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTc                                                                                                            >  2:1711249/1‑150 (MQ=255)
                                            aGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCa                                                                                                           <  1:1003526/150‑1 (MQ=255)
                                             tgATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCAt                                                                                                          <  2:137165/149‑1 (MQ=255)
                                             ggATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCAt                                                                                                          <  1:39477/150‑1 (MQ=255)
                                               atcatcTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGc                                                                                                        >  2:1228465/1‑150 (MQ=255)
                                                tcatcTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCt                                                                                                       <  2:1523508/150‑1 (MQ=255)
                                                 catcTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCtt                                                                                                      <  2:1485158/150‑1 (MQ=255)
                                                  atcTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTAGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTATGCTtga                                                                                                    >  1:1000726/1‑150 (MQ=255)
                                                    cTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTtgat                                                                                                   >  2:1665510/1‑150 (MQ=255)
                                                       gAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGaa                                                                                                >  1:737452/1‑150 (MQ=255)
                                                          aaaaTCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAAc                                                                                             <  1:949840/150‑1 (MQ=255)
                                                              tCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCATCGATCTGTCATGCTTGATGAAAACGGaa                                                                                         >  2:126322/1‑150 (MQ=255)
                                                              tCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGaa                                                                                         >  1:651724/1‑150 (MQ=255)
                                                               cTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAAt                                                                                        <  2:95053/150‑1 (MQ=255)
                                                               atttAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAAt                                                                                        <  1:955616/149‑1 (MQ=255)
                                                                tttAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAAtt                                                                                       >  2:602065/1‑150 (MQ=255)
                                                                tttAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAAtt                                                                                       <  2:177921/150‑1 (MQ=255)
                                                                  tAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGa                                                                                     <  1:1670216/150‑1 (MQ=255)
                                                                   aaCCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGaa                                                                                    >  2:585983/1‑150 (MQ=255)
                                                                   aaCCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGaa                                                                                    >  2:523802/1‑150 (MQ=255)
                                                                   aaCCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGaa                                                                                    >  2:1159401/1‑150 (MQ=255)
                                                                     ccATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTTTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGTAATTGAAcc                                                                                  <  2:752155/150‑1 (MQ=255)
                                                                          gTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCa                                                                             >  2:1031450/1‑150 (MQ=255)
                                                                           tCCGTTTTTTATCCCCTTGTTTTAATTTTACTATATGCTTGCTTAGTCGCTTTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCAGGCTTGATGAAAACGGAATTGAACCTGTCAt                                                                            <  1:1071446/150‑1 (MQ=255)
                                                                           tCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCAt                                                                            <  2:371176/150‑1 (MQ=255)
                                                                            ccGGTTTTTATCCCCTTGTATAAATATAACTAAATGCTTGCTTAGTCGCATAGTTAAATGCAATACAGTAAATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTAAtt                                                                           >  2:1311912/1‑150 (MQ=255)
                                                                                   ttATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTGGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGt                                                                    <  2:694989/150‑1 (MQ=255)
                                                                                    tATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTa                                                                   >  2:1265661/1‑150 (MQ=255)
                                                                                    tATCCCCTAGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTa                                                                   >  1:1367699/1‑150 (MQ=255)
                                                                                     aTCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAg                                                                  >  2:322227/1‑150 (MQ=255)
                                                                                      tCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGa                                                                 <  1:333174/150‑1 (MQ=255)
                                                                                        cccTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATAATCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATa                                                               <  1:592420/150‑1 (MQ=255)
                                                                                           ttGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATaaaa                                                            >  2:512645/1‑150 (MQ=255)
                                                                                            tGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATaaaaa                                                           <  1:1311407/150‑1 (MQ=255)
                                                                                              tataAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGc                                                         >  2:1369112/1‑150 (MQ=255)
                                                                                                 aaaTATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCTATATCATCTGTGCTGGCAACGATCAGTCATGCTTGATGAAAACGGAATTGAAACTGTCATTAACAGGTAGATAAAAAGCCtt                                                      >  1:591366/1‑149 (MQ=255)
                                                                                                      ttACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTTGCAACGATCTGTCATTCTTGATGAAAACGGAATTGAACCTTTCATTACCAGGAAGATAAAAAGCATGTTaaa                                                 >  1:686370/1‑150 (MQ=255)
                                                                                                        aCTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAAtt                                               >  1:61975/1‑150 (MQ=255)
                                                                                                         cTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAAttt                                              >  1:252573/1‑150 (MQ=255)
                                                                                                                    cttAGTCGCATTGTTAAATGCAATACCGTATATTCTGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTAttt                                   <  1:133222/150‑1 (MQ=255)
                                                                                                                      tattCGCATTGTTAAATGCAATACCTTATATTCGGTATTTAACATAGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTAttttt                                 <  1:311995/147‑1 (MQ=255)
                                                                                                                       aGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTAtttttt                                >  2:1455455/1‑150 (MQ=255)
                                                                                                                        gTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTta                               <  2:990597/150‑1 (MQ=255)
                                                                                                                         tCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTtat                              <  1:1159401/150‑1 (MQ=255)
                                                                                                                               tGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCg                        <  2:1405229/150‑1 (MQ=255)
                                                                                                                                gTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCgt                       >  1:1687600/1‑150 (MQ=255)
                                                                                                                                gTTAAATGCAATACAGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCgt                       >  1:1558166/1‑150 (MQ=255)
                                                                                                                                    aaTGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCt                   >  1:1257811/1‑150 (MQ=255)
                                                                                                                                     gttacaTACCGAATATACGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTg                  >  1:761679/6‑150 (MQ=255)
                                                                                                                                     aTGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCtt                  >  2:354638/1‑149 (MQ=255)
                                                                                                                                     aTGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTg                  <  2:1138435/150‑1 (MQ=255)
                                                                                                                                     aTGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGt                 <  2:1000726/150‑1 (MQ=255)
                                                                                                                                      ttcAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAATCGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGt                 <  1:114198/148‑1 (MQ=255)
                                                                                                                                      tggaaTACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGt                 <  2:326970/147‑1 (MQ=255)
                                                                                                                                      tGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGt                 <  1:379882/150‑1 (MQ=255)
                                                                                                                                        cAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTa               >  2:1608823/1‑150 (MQ=255)
                                                                                                                                         caTACCGAATATACGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGt                 <  2:155609/146‑1 (MQ=255)
                                                                                                                                         caTACCGAATATACGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGt                 >  1:155609/2‑147 (MQ=255)
                                                                                                                                           tACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATgg            <  1:1089165/150‑1 (MQ=255)
                                                                                                                                            aCCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATGGc           >  1:763836/1‑150 (MQ=255)
                                                                                                                                            aCCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATGGc           >  1:1089548/1‑150 (MQ=255)
                                                                                                                                            aCCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATGGc           <  2:816140/150‑1 (MQ=255)
                                                                                                                                             ccGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATGGCg          <  1:398827/150‑1 (MQ=255)
                                                                                                                                              cGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGTTAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATGGCGa         >  1:639725/1‑150 (MQ=255)
                                                                                                                                                    ttCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATGGCGACACggg   <  1:1711249/150‑1 (MQ=255)
                                                                                                                                                     tCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATGGCGACACGGGa  <  1:741143/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTGATGCCTGGGGGAGAAAGACCCATTAGTTAAGATTTACGCCAGGATCATCTTGAAAAAATCTTTAACCATGGTCCGGTTTTTATCCCCTTGTATAAATATTACTAAATGCTTGCTTAGTCGCATTGTTAAATGCAATACCGTATATTCGGTATGTAACAGCGATATCCTCTGTGCTGGCAACGATCTGTCATGCTTGATGAAAACGGAATTGAACCTGTCATTACCAGGTAGATAAAAAGCCTGTTCAATTTTGGGATTATTTTTTATAACTCGTGTCTGTTATGGCGACACGGGA  >  NC_000913/2387443‑2387741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: