Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,399,878 C→G 7.0% M96I (ATG→ATC) ‡ nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,399,8780CG 7.0% 291.8 / 7.9 94M96I (ATG→ATC) ‡nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base C (44/43);  new base G (2/4);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGC  >  NC_000913/2399730‑2400021
                                                                                                                                                    |                                                                                                                                               
gagagaTGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtca                                                                                                                                                <  2:1693648/150‑1 (MQ=255)
    gaTGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCaa                                                                                                                                            >  2:473979/1‑150 (MQ=255)
       tattGGCCCCACATCCTGATTACGGTGTGTACGTTTGGGGAATCGGTAGCGTCGGAAGCTGAGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCCGACAGTCGTGCGGGTGGTTGGTCATCAAcaa                                                                                                                                         <  2:1720408/147‑3 (MQ=255)
       gAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCa                                                                                                                                         <  1:79564/150‑1 (MQ=255)
         aTGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCAcc                                                                                                                                       <  1:1478412/150‑2 (MQ=255)
           ggCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCg                                                                                                                                     <  1:1250526/150‑1 (MQ=255)
            gCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGa                                                                                                                                    >  1:801318/1‑150 (MQ=255)
              cccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCCATCGAcc                                                                                                                                  >  2:1265740/1‑150 (MQ=255)
              cccAAATACTTATGACGGTGGGTTCGTGGGGTGAAACGGTAGCGACGGAAGCTGTGTCAGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCGCGCTTCACATACCGGACAGGCGTGCGAGTGGTTGATGACCAACCACACgcac                                                                                                                                  <  2:1577734/150‑4 (MQ=255)
               ccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCACCCgcacg                                                                                                                                 <  1:1270555/150‑5 (MQ=255)
               ccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCACCCgcacg                                                                                                                                 <  1:1277185/150‑5 (MQ=255)
                cAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCAc                                                                                                                                <  1:1010267/150‑1 (MQ=255)
                 aaaTCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGAGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGAGGGTGGTTGGTCATCAACCACTCGACCACg                                                                                                                               >  1:606363/1‑150 (MQ=255)
                   aTCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCCTATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCt                                                                                                                             <  2:837193/150‑1 (MQ=255)
                     ccagATTACGGTGGGAACGTTTGGTGAAACGGTAGCGACGGAAGCTGGGTCCGGTCATCACAGTCATATCCAGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGGGGATGGTCATCAACCACTCGACCACGCttt                                                                                                                           <  1:287404/147‑1 (MQ=255)
                      cTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTc                                                                                                                          <  2:106351/150‑1 (MQ=255)
                      cTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGGCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTc                                                                                                                          <  2:1062046/150‑1 (MQ=255)
                              ggTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACt                                                                                                                  <  1:1311707/150‑1 (MQ=255)
                              ggTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACt                                                                                                                  >  2:1216354/1‑150 (MQ=255)
                                gggggACGGTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGt                                                                                                                <  2:328790/149‑1 (MQ=255)
                                 gggTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGtt                                                                                                               >  2:34180/1‑150 (MQ=255)
                                    tACGTTTGGTGAAACGGTAGCGACGGAAGCTGGGGCCGGTCATCACAGTCATATCCTGAACATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCg                                                                                                            <  2:1335161/150‑1 (MQ=255)
                                       gTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGGTTCGcta                                                                                                         >  1:1441310/1‑149 (MQ=255)
                                           ggggAAACGGTAGCGACGTAAGCTGTGTCCGGTCATCACAGTCATATCCGGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                                                                     <  2:836741/147‑1 (MQ=255)
                                           ggTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGcttctta                                                                                                     >  2:1369093/1‑149 (MQ=255)
                                           ggTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                                                                     <  2:137845/150‑1 (MQ=255)
                                               aaaCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcgtcg                                                                                                 <  2:890233/150‑1 (MQ=255)
                                               aaaCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTATTCACATACAGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGCCCACGCTTTCACGGAACTGTTTCGCTTCTtcgtcg                                                                                                 >  1:289513/1‑150 (MQ=255)
                                                aaCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTcgtcgt                                                                                                >  2:1104964/1‑150 (MQ=255)
                                                 aCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCgtcgtc                                                                                               <  1:189918/150‑1 (MQ=255)
                                                 aCGGTAGCGACAGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCgtcgtc                                                                                               <  2:1092755/150‑1 (MQ=255)
                                                   ggTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCaa                                                                                             >  2:989156/1‑150 (MQ=255)
                                                   ggTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCaa                                                                                             >  1:1025811/1‑150 (MQ=255)
                                                      aGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAATATGGCAGTTACCGCCCTCTTCACATTCCGGACAGTCGGGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATgg                                                                                          <  1:1115565/150‑1 (MQ=255)
                                                         gACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCACCCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaaa                                                                                       >  2:1499479/1‑150 (MQ=255)
                                                           cGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATa                                                                                     >  1:1032110/1‑150 (MQ=255)
                                                             gAAGCTGGGTCCGGGCATCACAGTCATATCCGGAAGATGGCAGTTACCGCCCGCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCTACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGGCAATGGAAATaaa                                                                                   <  2:219514/150‑1 (MQ=255)
                                                               aGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAgg                                                                                 >  2:473041/1‑150 (MQ=255)
                                                               aGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAgg                                                                                 >  2:1486857/1‑150 (MQ=255)
                                                               aGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGGGCGGGTGGTGGGGCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCATTGGAAATAAAgg                                                                                 <  2:66643/150‑1 (MQ=255)
                                                               aGCTGTGGCCGGTCATCACAGTCATATCCTGAAGAGTGCAGTTACCGCCCTCTTCACCTACCGGACAGTCGGGCGGGTGGTTGGGCATCAACCACGCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAgg                                                                                 <  1:695582/150‑1 (MQ=255)
                                                                     gtCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACAACGCATTCACGGAACTGTTTCGCTTCATCCTCGTCAATGGAAATAAAGATGCCAt                                                                           >  2:334753/1‑150 (MQ=255)
                                                                      tCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATc                                                                          <  1:513883/150‑1 (MQ=255)
                                                                       ccGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                                                                         >  1:1684057/1‑150 (MQ=255)
                                                                        cGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCATAGGAAATAAAGGTGCCATCgg                                                                        >  1:225369/1‑150 (MQ=255)
                                                                        cGGGCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCgg                                                                        <  2:1211533/150‑1 (MQ=255)
                                                                           tcatcaCAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAg                                                                     >  1:639294/1‑150 (MQ=255)
                                                                               cacaGTCATATCCTGAAGATGGCAGTTACCGCCCTCATCACATCCCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCgg                                                                 <  2:1342891/150‑1 (MQ=255)
                                                                                acaGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTGTCGCGTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAGGCCGgt                                                                >  1:225191/1‑150 (MQ=255)
                                                                                 caGTCATTTCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                                                               >  1:1322382/1‑150 (MQ=255)
                                                                                 caGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                                                               <  1:1294956/150‑1 (MQ=255)
                                                                                      ggtatgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCTCGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATa                                                          >  2:985566/7‑150 (MQ=255)
                                                                                       tatCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATAc                                                         <  2:1032110/150‑1 (MQ=255)
                                                                                          tgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCCTCAACCTCTCGACCACGCATTCACGGAACTGTGTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                      >  1:887023/3‑150 (MQ=255)
                                                                                          ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                      <  2:769920/150‑1 (MQ=255)
                                                                                          ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                      >  1:664208/1‑150 (MQ=255)
                                                                                          ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                      >  2:1711431/1‑150 (MQ=255)
                                                                                          ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                      <  1:1556976/150‑1 (MQ=255)
                                                                                            tGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGATATTAAGGTGCCATCGGAAGCCGGTGTCATACAGGAc                                                    >  1:1096636/1‑150 (MQ=255)
                                                                                            tGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCATCTTCTTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGAc                                                    <  1:1216354/150‑1 (MQ=255)
                                                                                             gAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACa                                                   <  2:801318/150‑1 (MQ=255)
                                                                                                gATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCATTCACCGAACTGATTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACaaaa                                                >  2:92678/1‑147 (MQ=255)
                                                                                                  tGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAcc                                              >  2:703399/1‑150 (MQ=255)
                                                                                                   ggCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCa                                             >  2:618842/1‑150 (MQ=255)
                                                                                                   ggCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCa                                             >  2:612771/1‑150 (MQ=255)
                                                                                                    gCAGTTACCGCCCTCTTCACATACCTGACAGGCGTGGGGGTGGTTGGTCATCACCCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCTATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAg                                            <  1:169282/150‑1 (MQ=255)
                                                                                                    gCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCATCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCTGTGTCATACAGGACATCACCAg                                            <  2:1216014/150‑1 (MQ=255)
                                                                                                     cAGTTACCGCCCTCTTCACATACCGGACAGTGGTGCGGGTGGTGGGTCATCAAGCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTGAATGGTAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAgg                                           <  1:360662/150‑1 (MQ=255)
                                                                                                      aGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGc                                          >  2:1297746/1‑150 (MQ=255)
                                                                                                      aGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGGCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGc                                          <  1:34180/150‑1 (MQ=255)
                                                                                                           ccGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCa                                     >  2:403549/1‑150 (MQ=255)
                                                                                                             gCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGACGTGTTTCGATTCTTCGTCGTCAATGGAAATTAAGGTGCCATCGGGAGCCGGTGGGATTCAGGACATCGCCCGGCGCCCTcg                                   >  2:256816/1‑150 (MQ=255)
                                                                                                             gCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATATAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGAGCAcg                                   >  2:203682/1‑150 (MQ=255)
                                                                                                               ccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACGCGACCTCGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcgcg                                 <  1:256816/150‑1 (MQ=255)
                                                                                                               ccTCTTCACATACCGGACAGTCGGGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcgcg                                 <  2:1665308/150‑1 (MQ=255)
                                                                                                                 tcatCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAACTAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCgtg                               <  1:456669/147‑1 (MQ=255)
                                                                                                                       caTACCGGACAGTCGTGAGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTcc                         <  2:246389/150‑1 (MQ=255)
                                                                                                                           ccGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCAAGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACAACGCGAGTCTTCCCCGt                     >  2:72718/1‑150 (MQ=255)
                                                                                                                             ggACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGCCATCCCCAGGCGACCACGCGTGTCTTCCGCGttt                   >  1:1143847/1‑150 (MQ=255)
                                                                                                                              gACAGTCGTGCGGGTGGTTGGTCATCAACCACGCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGtttt                  <  2:711417/150‑1 (MQ=255)
                                                                                                                               aCAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTg                 <  1:1104964/150‑1 (MQ=255)
                                                                                                                                cAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTgg                >  2:576737/1‑150 (MQ=255)
                                                                                                                                 aGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCGCGCGTGTCTTCCGCGTTTTGGt               >  1:43459/1‑150 (MQ=255)
                                                                                                                                    cGTGCGGGTGGTTGGTCATCAACCCCTAGACCACGCTTTCACGGACCTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAtt            >  2:1410055/1‑150 (MQ=255)
                                                                                                                                    cGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAtt            >  1:87910/1‑150 (MQ=255)
                                                                                                                                      gtcgagtggtTGATGACCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGc          >  1:1125219/6‑150 (MQ=255)
                                                                                                                                       tcgagtggtTGATTACCAACCACTAGACCACGCTTTCACGGAACTGTTTCGATTCTTCGTCCTCAAAGCAAATAAAGGTGCAATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCTTGTCTTCCGCGTTTTGGTATTGCt         >  2:1282205/5‑150 (MQ=255)
                                                                                                                                       gCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCt         <  1:989156/150‑1 (MQ=255)
                                                                                                                                       gCGGGTGGTTGGGCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCTATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCt         <  2:864585/150‑1 (MQ=255)
                                                                                                                                        cgagtggtTGATGACCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCtt        >  1:1715717/4‑150 (MQ=255)
                                                                                                                                         gggTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTc       >  2:4554/1‑150 (MQ=255)
                                                                                                                                          ggtggtTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCa      >  2:147184/1‑150 (MQ=255)
                                                                                                                                           gtggtTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCAc     <  1:951365/150‑1 (MQ=255)
                                                                                                                                             ggttggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCg   <  2:664208/150‑1 (MQ=255)
                                                                                                                                              gttggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGc  >  2:1302590/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGC  >  NC_000913/2399730‑2400021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑