Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,399,880 T→C 7.4% M96V (ATG→GTG) ‡ nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,399,8800TC 7.4% 266.9 / 9.5 94M96V (ATG→GTG) ‡nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base T (44/43);  new base C (3/4);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGG  >  NC_000913/2399734‑2400028
                                                                                                                                                  |                                                                                                                                                    
gaTGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCaa                                                                                                                                                   >  2:473979/1‑150 (MQ=255)
   tattGGCCCCACATCCTGATTACGGTGTGTACGTTTGGGGAATCGGTAGCGTCGGAAGCTGAGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCCGACAGTCGTGCGGGTGGTTGGTCATCAAcaa                                                                                                                                                <  2:1720408/147‑3 (MQ=255)
   gAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCa                                                                                                                                                <  1:79564/150‑1 (MQ=255)
     aTGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCAcc                                                                                                                                              <  1:1478412/150‑2 (MQ=255)
       ggCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCg                                                                                                                                            <  1:1250526/150‑1 (MQ=255)
        gCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGa                                                                                                                                           >  1:801318/1‑150 (MQ=255)
          cccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCCATCGAcc                                                                                                                                         >  2:1265740/1‑150 (MQ=255)
          cccAAATACTTATGACGGTGGGTTCGTGGGGTGAAACGGTAGCGACGGAAGCTGTGTCAGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCGCGCTTCACATACCGGACAGGCGTGCGAGTGGTTGATGACCAACCACACgcac                                                                                                                                         <  2:1577734/150‑4 (MQ=255)
           ccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCACCCgcacg                                                                                                                                        <  1:1270555/150‑5 (MQ=255)
           ccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCACCCgcacg                                                                                                                                        <  1:1277185/150‑5 (MQ=255)
            cAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCAc                                                                                                                                       <  1:1010267/150‑1 (MQ=255)
             aaaTCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGAGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGAGGGTGGTTGGTCATCAACCACTCGACCACg                                                                                                                                      >  1:606363/1‑150 (MQ=255)
               aTCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCCTATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCt                                                                                                                                    <  2:837193/150‑1 (MQ=255)
                 ccagATTACGGTGGGAACGTTTGGTGAAACGGTAGCGACGGAAGCTGGGTCCGGTCATCACAGTCATATCCAGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGGGGATGGTCATCAACCACTCGACCACGCttt                                                                                                                                  <  1:287404/147‑1 (MQ=255)
                  cTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTc                                                                                                                                 <  2:106351/150‑1 (MQ=255)
                  cTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGGCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTc                                                                                                                                 <  2:1062046/150‑1 (MQ=255)
                          ggTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACt                                                                                                                         >  2:1216354/1‑150 (MQ=255)
                          ggTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACt                                                                                                                         <  1:1311707/150‑1 (MQ=255)
                            gggggACGGTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGt                                                                                                                       <  2:328790/149‑1 (MQ=255)
                             gggTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGtt                                                                                                                      >  2:34180/1‑150 (MQ=255)
                                tACGTTTGGTGAAACGGTAGCGACGGAAGCTGGGGCCGGTCATCACAGTCATATCCTGAACATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCg                                                                                                                   <  2:1335161/150‑1 (MQ=255)
                                   gTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGGTTCGcta                                                                                                                >  1:1441310/1‑149 (MQ=255)
                                       ggggAAACGGTAGCGACGTAAGCTGTGTCCGGTCATCACAGTCATATCCGGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                                                                            <  2:836741/147‑1 (MQ=255)
                                       ggTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGcttctta                                                                                                            >  2:1369093/1‑149 (MQ=255)
                                       ggTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                                                                            <  2:137845/150‑1 (MQ=255)
                                           aaaCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcgtcg                                                                                                        <  2:890233/150‑1 (MQ=255)
                                           aaaCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTATTCACATACAGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGCCCACGCTTTCACGGAACTGTTTCGCTTCTtcgtcg                                                                                                        >  1:289513/1‑150 (MQ=255)
                                            aaCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTcgtcgt                                                                                                       >  2:1104964/1‑150 (MQ=255)
                                             aCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCgtcgtc                                                                                                      <  1:189918/150‑1 (MQ=255)
                                             aCGGTAGCGACAGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCgtcgtc                                                                                                      <  2:1092755/150‑1 (MQ=255)
                                               ggTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCaa                                                                                                    >  2:989156/1‑150 (MQ=255)
                                               ggTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCaa                                                                                                    >  1:1025811/1‑150 (MQ=255)
                                                  aGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAATATGGCAGTTACCGCCCTCTTCACATTCCGGACAGTCGGGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATgg                                                                                                 <  1:1115565/150‑1 (MQ=255)
                                                     gACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCACCCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaaa                                                                                              >  2:1499479/1‑150 (MQ=255)
                                                       cGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATa                                                                                            >  1:1032110/1‑150 (MQ=255)
                                                         gAAGCTGGGTCCGGGCATCACAGTCATATCCGGAAGATGGCAGTTACCGCCCGCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCTACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGGCAATGGAAATaaa                                                                                          <  2:219514/150‑1 (MQ=255)
                                                           aGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAgg                                                                                        >  2:473041/1‑150 (MQ=255)
                                                           aGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAgg                                                                                        >  2:1486857/1‑150 (MQ=255)
                                                           aGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGGGCGGGTGGTGGGGCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCATTGGAAATAAAgg                                                                                        <  2:66643/150‑1 (MQ=255)
                                                           aGCTGTGGCCGGTCATCACAGTCATATCCTGAAGAGTGCAGTTACCGCCCTCTTCACCTACCGGACAGTCGGGCGGGTGGTTGGGCATCAACCACGCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAgg                                                                                        <  1:695582/150‑1 (MQ=255)
                                                                 gtCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACAACGCATTCACGGAACTGTTTCGCTTCATCCTCGTCAATGGAAATAAAGATGCCAt                                                                                  >  2:334753/1‑150 (MQ=255)
                                                                  tCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATc                                                                                 <  1:513883/150‑1 (MQ=255)
                                                                   ccGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                                                                                >  1:1684057/1‑150 (MQ=255)
                                                                    cGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCATAGGAAATAAAGGTGCCATCgg                                                                               >  1:225369/1‑150 (MQ=255)
                                                                    cGGGCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCgg                                                                               <  2:1211533/150‑1 (MQ=255)
                                                                       tcatcaCAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAg                                                                            >  1:639294/1‑150 (MQ=255)
                                                                           cacaGTCATATCCTGAAGATGGCAGTTACCGCCCTCATCACATCCCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCgg                                                                        <  2:1342891/150‑1 (MQ=255)
                                                                            acaGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTGTCGCGTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAGGCCGgt                                                                       >  1:225191/1‑150 (MQ=255)
                                                                             caGTCATTTCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                                                                      >  1:1322382/1‑150 (MQ=255)
                                                                             caGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                                                                      <  1:1294956/150‑1 (MQ=255)
                                                                                  ggtatgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCTCGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATa                                                                 >  2:985566/7‑150 (MQ=255)
                                                                                   tatCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATAc                                                                <  2:1032110/150‑1 (MQ=255)
                                                                                      tgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCCTCAACCTCTCGACCACGCATTCACGGAACTGTGTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                             >  1:887023/3‑150 (MQ=255)
                                                                                      ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                             >  1:664208/1‑150 (MQ=255)
                                                                                      ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                             <  2:769920/150‑1 (MQ=255)
                                                                                      ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                             <  1:1556976/150‑1 (MQ=255)
                                                                                      ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                             >  2:1711431/1‑150 (MQ=255)
                                                                                        tGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGATATTAAGGTGCCATCGGAAGCCGGTGTCATACAGGAc                                                           >  1:1096636/1‑150 (MQ=255)
                                                                                        tGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCATCTTCTTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGAc                                                           <  1:1216354/150‑1 (MQ=255)
                                                                                         gAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACa                                                          <  2:801318/150‑1 (MQ=255)
                                                                                            gATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCATTCACCGAACTGATTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACaaaa                                                       >  2:92678/1‑147 (MQ=255)
                                                                                              tGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAcc                                                     >  2:703399/1‑150 (MQ=255)
                                                                                               ggCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCa                                                    >  2:618842/1‑150 (MQ=255)
                                                                                               ggCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCa                                                    >  2:612771/1‑150 (MQ=255)
                                                                                                gCAGTTACCGCCCTCTTCACATACCTGACAGGCGTGGGGGTGGTTGGTCATCACCCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCTATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAg                                                   <  1:169282/150‑1 (MQ=255)
                                                                                                gCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCATCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCTGTGTCATACAGGACATCACCAg                                                   <  2:1216014/150‑1 (MQ=255)
                                                                                                 cAGTTACCGCCCTCTTCACATACCGGACAGTGGTGCGGGTGGTGGGTCATCAAGCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTGAATGGTAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAgg                                                  <  1:360662/150‑1 (MQ=255)
                                                                                                  aGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGc                                                 >  2:1297746/1‑150 (MQ=255)
                                                                                                  aGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGGCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGc                                                 <  1:34180/150‑1 (MQ=255)
                                                                                                       ccGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCa                                            >  2:403549/1‑150 (MQ=255)
                                                                                                         gCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGACGTGTTTCGATTCTTCGTCGTCAATGGAAATTAAGGTGCCATCGGGAGCCGGTGGGATTCAGGACATCGCCCGGCGCCCTcg                                          >  2:256816/1‑150 (MQ=255)
                                                                                                         gCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATATAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGAGCAcg                                          >  2:203682/1‑150 (MQ=255)
                                                                                                           ccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACGCGACCTCGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcgcg                                        <  1:256816/150‑1 (MQ=255)
                                                                                                           ccTCTTCACATACCGGACAGTCGGGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcgcg                                        <  2:1665308/150‑1 (MQ=255)
                                                                                                             tcatCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAACTAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCgtg                                      <  1:456669/147‑1 (MQ=255)
                                                                                                                   caTACCGGACAGTCGTGAGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTcc                                <  2:246389/150‑1 (MQ=255)
                                                                                                                       ccGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCAAGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACAACGCGAGTCTTCCCCGt                            >  2:72718/1‑150 (MQ=255)
                                                                                                                         ggACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGCCATCCCCAGGCGACCACGCGTGTCTTCCGCGttt                          >  1:1143847/1‑150 (MQ=255)
                                                                                                                          gACAGTCGTGCGGGTGGTTGGTCATCAACCACGCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGtttt                         <  2:711417/150‑1 (MQ=255)
                                                                                                                           aCAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTg                        <  1:1104964/150‑1 (MQ=255)
                                                                                                                            cAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTgg                       >  2:576737/1‑150 (MQ=255)
                                                                                                                             aGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCGCGCGTGTCTTCCGCGTTTTGGt                      >  1:43459/1‑150 (MQ=255)
                                                                                                                                cGTGCGGGTGGTTGGTCATCAACCCCTAGACCACGCTTTCACGGACCTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAtt                   >  2:1410055/1‑150 (MQ=255)
                                                                                                                                cGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAtt                   >  1:87910/1‑150 (MQ=255)
                                                                                                                                  gtcgagtggtTGATGACCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGc                 >  1:1125219/6‑150 (MQ=255)
                                                                                                                                   tcgagtggtTGATTACCAACCACTAGACCACGCTTTCACGGAACTGTTTCGATTCTTCGTCCTCAAAGCAAATAAAGGTGCAATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCTTGTCTTCCGCGTTTTGGTATTGCt                >  2:1282205/5‑150 (MQ=255)
                                                                                                                                   gCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCt                <  1:989156/150‑1 (MQ=255)
                                                                                                                                   gCGGGTGGTTGGGCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCTATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCt                <  2:864585/150‑1 (MQ=255)
                                                                                                                                    cgagtggtTGATGACCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCtt               >  1:1715717/4‑150 (MQ=255)
                                                                                                                                     gggTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTc              >  2:4554/1‑150 (MQ=255)
                                                                                                                                      ggtggtTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCa             >  2:147184/1‑150 (MQ=255)
                                                                                                                                       gtggtTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCAc            <  1:951365/150‑1 (MQ=255)
                                                                                                                                         ggttggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCg          <  2:664208/150‑1 (MQ=255)
                                                                                                                                          gttggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGc         >  2:1302590/1‑150 (MQ=255)
                                                                                                                                                 atcaACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTgg  >  1:908750/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTGG  >  NC_000913/2399734‑2400028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑