Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,412,640 G→T 6.2% intergenic (‑46/+37) yfbU ← / ← yfbV UPF0304 family protein/UPF0208 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,412,6400GT 6.2% 390.4 / 10.5 131intergenic (‑46/+37)yfbU/yfbVUPF0304 family protein/UPF0208 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (51/71);  new base T (7/2);  total (58/73)
Fisher's exact test for biased strand distribution p-value = 7.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGAC  >  NC_000913/2412493‑2412788
                                                                                                                                                   |                                                                                                                                                    
tGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAg                                                                                                                                                    >  1:1101409/1‑150 (MQ=255)
 gTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGt                                                                                                                                                   <  2:709665/150‑1 (MQ=255)
     gCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGc                                                                                                                                               >  2:1510152/1‑150 (MQ=255)
      cAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGcc                                                                                                                                              <  2:1473882/150‑1 (MQ=255)
      cAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGca                                                                                                                                              >  2:709281/1‑149 (MQ=255)
            ccGGGAACGTTCAGCAGTTGCCGGATCGAGCATAGTCATCCTCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGGCATTTACATTGTTGACTCCGGTATCCCTCTACTACGGGGAAAAAAAAGTCGGCGGAGTATGCCTTCttt                                                                                                                                        <  2:205049/149‑1 (MQ=255)
            aCGGTAACGTTCAGCATTTGCCGGTTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCttt                                                                                                                                        <  2:931271/150‑1 (MQ=255)
             cGGTAACTTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCATACTCAGCCTTCtttt                                                                                                                                       <  1:532598/150‑1 (MQ=255)
             cGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTTCTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATGACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCtttt                                                                                                                                       >  1:116276/1‑150 (MQ=255)
             cGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAAAAATACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATACCTTCtttt                                                                                                                                       <  2:1144143/150‑1 (MQ=255)
                ttaCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCATGTACTGATTAGACAAAATAAGTCGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                    <  2:1627976/148‑1 (MQ=255)
                tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                    <  1:1000357/150‑1 (MQ=255)
                tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                    <  1:372949/150‑1 (MQ=255)
                tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                    >  1:574907/1‑150 (MQ=255)
                tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                    <  1:596596/150‑1 (MQ=255)
                 aaCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTatat                                                                                                                                   <  1:532498/150‑1 (MQ=255)
                  aCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATg                                                                                                                                  <  1:1297544/150‑1 (MQ=255)
                   cGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGc                                                                                                                                 >  2:526525/1‑150 (MQ=255)
                       aatcATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTaa                                                                                                                             <  1:196477/147‑1 (MQ=255)
                         gCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATc                                                                                                                           >  1:894979/1‑150 (MQ=255)
                         gCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATc                                                                                                                           <  1:1360418/150‑1 (MQ=255)
                                ccGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTc                                                                                                                    <  1:1698115/150‑1 (MQ=255)
                                    ttCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAAtt                                                                                                                <  1:1664164/149‑1 (MQ=255)
                                    aTCGAGCATAGTCATCAACTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAAtt                                                                                                                <  2:558069/150‑1 (MQ=255)
                                            tAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTTACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCGTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatca                                                                                                        >  2:523328/1‑150 (MQ=255)
                                              gTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatcatc                                                                                                      >  2:1534159/1‑150 (MQ=255)
                                              gTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTATACTACGGTGAAAAAAAAGAAGGCTGAGTATGACTTCTTTTATATGAGTAATCAGGGGTCAATTAAAAatcatc                                                                                                      >  1:1037623/1‑150 (MQ=255)
                                                catcatCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTTGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                                                    <  1:36132/150‑1 (MQ=255)
                                                 atcatcTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAg                                                                                                   <  2:296468/150‑1 (MQ=255)
                                                       ttGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGt                                                                                             >  1:1346313/1‑150 (MQ=255)
                                                        tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtt                                                                                            >  2:1437099/1‑150 (MQ=255)
                                                        tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtt                                                                                            >  2:161589/1‑150 (MQ=255)
                                                        tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGAGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtt                                                                                            >  2:1443723/1‑150 (MQ=255)
                                                         ttACTGATTAGACAAAATAAGACGTTGCGCGTTCGTCATTTCCATTGTTGACTCCTTTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGGCAATTACAAATCATCAAGGAAAGttt                                                                                           <  1:953940/149‑1 (MQ=255)
                                                         gTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGttt                                                                                           <  2:385910/150‑1 (MQ=255)
                                                         gTACTGATTAGACAAAATAAGACGTTGCGCGTTGGGCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGttt                                                                                           <  2:155718/150‑1 (MQ=255)
                                                            cTGATTAGACAAAATAAGACGTTGCGCGTTGGTCCTTTCCATTGTTGACTCCTGTATAACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAATTTTTAt                                                                                        >  1:1523170/1‑150 (MQ=255)
                                                             tGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATc                                                                                       >  1:576428/1‑150 (MQ=255)
                                                             tGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATc                                                                                       <  1:1166117/150‑1 (MQ=255)
                                                              gATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATcc                                                                                      <  2:1441229/150‑1 (MQ=255)
                                                                ttAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAg                                                                                    >  1:1074134/1‑150 (MQ=255)
                                                                 tAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATTACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgt                                                                                   <  2:1156509/150‑1 (MQ=255)
                                                                  aGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgtt                                                                                  >  2:1723837/1‑150 (MQ=255)
                                                                   gACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttg                                                                                 <  1:1602048/150‑1 (MQ=255)
                                                                     cAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgtt                                                                               >  1:1518405/1‑150 (MQ=255)
                                                                     cAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgtt                                                                               >  2:1325632/1‑150 (MQ=255)
                                                                       aaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTg                                                                             <  2:453810/150‑1 (MQ=255)
                                                                          tAAGACGTTGCGCGTTTGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGTCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                          <  1:852625/150‑1 (MQ=255)
                                                                          tAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                          <  2:184946/150‑1 (MQ=255)
                                                                             gACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGTCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcg                                                                       >  2:1353873/1‑150 (MQ=255)
                                                                              aCGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgc                                                                      >  2:789620/1‑150 (MQ=255)
                                                                               cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                          >  1:828416/1‑145 (MQ=255)
                                                                               cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                          <  2:828416/145‑1 (MQ=255)
                                                                               cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgcg                                                                     <  2:12242/150‑1 (MQ=255)
                                                                               cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgcg                                                                     <  2:429655/150‑1 (MQ=255)
                                                                                          ggTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGAGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATACAGTTGTTTGAAGGCGCGCTTAATCgtgt                                                          >  1:1194234/1‑150 (MQ=255)
                                                                                           gTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc                                                         >  1:416838/1‑150 (MQ=255)
                                                                                              aTTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGc                                                      <  1:624040/150‑1 (MQ=255)
                                                                                                 tCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAAATACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTaa                                                   >  1:250061/1‑150 (MQ=255)
                                                                                                 tCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGAGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTaa                                                   >  1:272088/1‑150 (MQ=255)
                                                                                                  ccATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAAt                                                  <  1:1585734/150‑1 (MQ=255)
                                                                                                   cATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatcatc                                                                                                      <  1:1720806/97‑1 (MQ=255)
                                                                                                   cATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCGGAGTATGCCTTCTTTTATATGCGTAATCAGGGGGCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATg                                                 <  2:244770/150‑1 (MQ=255)
                                                                                                       gttgACTCCTGTATCACTCTACTACTTTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTg                                             <  2:1037623/150‑1 (MQ=255)
                                                                                                          gACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTa                                          >  2:926186/1‑150 (MQ=255)
                                                                                                          gACTCCTGTATCACTCTACTACGGTGAAAAAAAGAAGGCTGAGTATGGCTTCTTTTATATGCGTAATCAGGGGTGATTTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTaa                                         >  2:922916/1‑150 (MQ=255)
                                                                                                           acacttGTATCAATCTACTACGGTCAAATAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTATTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTaa                                         <  1:1092436/145‑1 (MQ=255)
                                                                                                           aCTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTaa                                         >  2:953007/1‑150 (MQ=255)
                                                                                                            cTCCTGTATCACTCTACTACTGTGAAAAAAAAGAAGGCTGAGTATGGCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAAt                                        <  2:237576/150‑1 (MQ=255)
                                                                                                            cTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAAt                                        <  2:1143238/150‑1 (MQ=255)
                                                                                                                tGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGGCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg                                    <  1:515497/150‑1 (MQ=255)
                                                                                                                tGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg                                    >  1:1025737/1‑150 (MQ=255)
                                                                                                                  tATCACTCTACTACGGTGAAAAAAAAGACGGCGGAGTATGCCTTCTTTTATATGCGTAACCAGGGGGCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTTAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt                                  <  2:864408/150‑1 (MQ=255)
                                                                                                                    tCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCATTTGTTTGAAGGAGCGCTTAAGCGTGTCAGCTAATGCATGTTAATAAGGATTg                                >  1:841186/1‑150 (MQ=255)
                                                                                                                    tCACTCTACAACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTg                                <  2:672815/150‑1 (MQ=255)
                                                                                                                      aCTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGcc                              <  2:923954/150‑1 (MQ=255)
                                                                                                                       ctctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGATTACCAGGcattagc                             <  1:860188/150‑7 (MQ=255)
                                                                                                                        tctACTACGGTGAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTc                           <  2:1560682/150‑1 (MQ=255)
                                                                                                                              acGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACgg                      <  2:1074134/150‑1 (MQ=255)
                                                                                                                                ggTGAAAAAAAAGAAGTCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTTGTAATCAGGCTTGCCTTCAACGGGt                    <  1:768897/150‑1 (MQ=255)
                                                                                                                                 cgcatataaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTg                   >  2:1096560/8‑150 (MQ=255)
                                                                                                                                  tGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTAAACGGGTGc                  >  2:863140/1‑150 (MQ=255)
                                                                                                                                   gAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATTCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                 <  1:202144/150‑1 (MQ=255)
                                                                                                                                   gAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                 <  2:1518405/150‑1 (MQ=255)
                                                                                                                                   gAAAAAAAAGAAGGCTGAGTATCCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                 >  1:786547/1‑150 (MQ=255)
                                                                                                                                    aaaaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATAAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCa                >  2:1400129/1‑150 (MQ=255)
                                                                                                                                     aaaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCaa               >  2:2903/1‑150 (MQ=255)
                                                                                                                                      ataaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAac              >  2:1100606/3‑150 (MQ=255)
                                                                                                                                        aaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAacac            <  1:679130/150‑1 (MQ=255)
                                                                                                                                        aaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAacac            >  2:1681513/1‑150 (MQ=255)
                                                                                                                                        aaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAacac            >  1:298489/1‑150 (MQ=255)
                                                                                                                                         aaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAATGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACAcc           >  2:41359/1‑150 (MQ=255)
                                                                                                                                         aaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACAcc           >  1:686432/1‑150 (MQ=255)
                                                                                                                                         aaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCaaaccc           >  1:210343/1‑146 (MQ=255)
                                                                                                                                         aaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACAcc           >  1:1011687/1‑150 (MQ=255)
                                                                                                                                             aagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTATTGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTc       <  2:894979/150‑1 (MQ=255)
                                                                                                                                              agGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTcc      >  1:430430/1‑150 (MQ=255)
                                                                                                                                              agGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTcc      <  2:809068/150‑1 (MQ=255)
                                                                                                                                              agGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTcc      >  2:807226/1‑150 (MQ=255)
                                                                                                                                                  tGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGAc  >  1:746880/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGAC  >  NC_000913/2412493‑2412788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: