Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,412,642 G→C 6.9% intergenic (‑48/+35) yfbU ← / ← yfbV UPF0304 family protein/UPF0208 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,412,6420GC 6.9% 419.7 / 17.1 130intergenic (‑48/+35)yfbU/yfbVUPF0304 family protein/UPF0208 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (50/71);  new base C (7/2);  total (57/73)
Fisher's exact test for biased strand distribution p-value = 4.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

Only 100 of 132 total aligned reads displayed.

TGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCC  >  NC_000913/2412493‑2412791
                                                                                                                                                     |                                                                                                                                                     
tGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAg                                                                                                                                                       >  1:1101409/1‑150 (MQ=255)
 gTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGt                                                                                                                                                      <  2:709665/150‑1 (MQ=255)
     gCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGc                                                                                                                                                  >  2:1510152/1‑150 (MQ=255)
      cAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGca                                                                                                                                                 >  2:709281/1‑149 (MQ=255)
            ccGGGAACGTTCAGCAGTTGCCGGATCGAGCATAGTCATCCTCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGGCATTTACATTGTTGACTCCGGTATCCCTCTACTACGGGGAAAAAAAAGTCGGCGGAGTATGCCTTCttt                                                                                                                                           <  2:205049/149‑1 (MQ=255)
            aCGGTAACGTTCAGCATTTGCCGGTTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCttt                                                                                                                                           <  2:931271/150‑1 (MQ=255)
             cGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTTCTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATGACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCtttt                                                                                                                                          >  1:116276/1‑150 (MQ=255)
                ttaCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCATGTACTGATTAGACAAAATAAGTCGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                       <  2:1627976/148‑1 (MQ=255)
                tatagTTCAGCATTTGCCGGATCGAGCATAGTCCTCATCTTGTAATGATTAGACTATATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCATACTCAGCCTTCttttttt                                                                                                                                       <  1:619419/146‑4 (MQ=255)
                tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                       >  1:574907/1‑150 (MQ=255)
                tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                       <  1:372949/150‑1 (MQ=255)
                tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                       <  1:1000357/150‑1 (MQ=255)
                 aaCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTatat                                                                                                                                      <  1:532498/150‑1 (MQ=255)
                  aCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATg                                                                                                                                     <  1:1297544/150‑1 (MQ=255)
                   cGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGc                                                                                                                                    >  2:526525/1‑150 (MQ=255)
                   cGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGc                                                                                                                                    >  2:439074/1‑150 (MQ=255)
                    gTTCAGCATTTGCCGGATCGAGAATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCg                                                                                                                                   <  2:535739/150‑1 (MQ=255)
                       aatcATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTaa                                                                                                                                <  1:196477/147‑1 (MQ=255)
                         gCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATc                                                                                                                              <  1:1360418/150‑1 (MQ=255)
                                ccGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTc                                                                                                                       <  1:1698115/150‑1 (MQ=255)
                                    ttCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAAtt                                                                                                                   <  1:1664164/149‑1 (MQ=255)
                                            tAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTTACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCGTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatca                                                                                                           >  2:523328/1‑150 (MQ=255)
                                            tAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatca                                                                                                           >  1:477697/1‑150 (MQ=255)
                                              gTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTATACTACGGTGAAAAAAAAGAAGGCTGAGTATGACTTCTTTTATATGAGTAATCAGGGGTCAATTAAAAatcatc                                                                                                         >  1:1037623/1‑150 (MQ=255)
                                                catcatCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTTGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                                                       <  1:36132/150‑1 (MQ=255)
                                                catcatCTAGTACTGATTAGACAAAATAAGACGTTGCGCGTAGATCATTTCCATTGGTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                                                       <  2:301076/150‑1 (MQ=255)
                                                 atcatcTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAg                                                                                                      <  2:296468/150‑1 (MQ=255)
                                                       ttGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGt                                                                                                >  1:1346313/1‑150 (MQ=255)
                                                        tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtt                                                                                               >  2:1602048/1‑150 (MQ=255)
                                                        tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGAGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtt                                                                                               >  2:1443723/1‑150 (MQ=255)
                                                         ttACTGATTAGACAAAATAAGACGTTGCGCGTTCGTCATTTCCATTGTTGACTCCTTTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGGCAATTACAAATCATCAAGGAAAGttt                                                                                              <  1:953940/149‑1 (MQ=255)
                                                         gTACTGATTAGACAAAATAAGACGTTGCGCGTTGGGCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGttt                                                                                              <  2:155718/150‑1 (MQ=255)
                                                            cTGATTAGACAAAATAAGACGTTGCGCGTTGGTCCTTTCCATTGTTGACTCCTGTATAACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAATTTTTAt                                                                                           >  1:1523170/1‑150 (MQ=255)
                                                             tGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATc                                                                                          <  1:1166117/150‑1 (MQ=255)
                                                             tGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATc                                                                                          >  1:576428/1‑150 (MQ=255)
                                                              gATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATcc                                                                                         <  2:1441229/150‑1 (MQ=255)
                                                                ttAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAg                                                                                       >  1:1074134/1‑150 (MQ=255)
                                                                 tAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATTACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgt                                                                                      <  2:1156509/150‑1 (MQ=255)
                                                                   gACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttg                                                                                    >  2:1447719/1‑150 (MQ=255)
                                                                     cAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgtt                                                                                  >  1:1518405/1‑150 (MQ=255)
                                                                     aaaaaTATGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgtt                                                                                  <  2:1070706/149‑1 (MQ=255)
                                                                      aaaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGttt                                                                                 <  1:1612248/150‑1 (MQ=255)
                                                                       aaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTg                                                                                <  2:1403262/150‑1 (MQ=255)
                                                                          tAAGACGTTGCGCGTTTGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGTCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                             <  1:852625/150‑1 (MQ=255)
                                                                          tAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                             <  2:184946/150‑1 (MQ=255)
                                                                             gACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGTCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcg                                                                          >  2:1353873/1‑150 (MQ=255)
                                                                              aCGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgc                                                                         >  2:789620/1‑150 (MQ=255)
                                                                               cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                             >  1:828416/1‑145 (MQ=255)
                                                                               cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                             <  2:828416/145‑1 (MQ=255)
                                                                               cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgcg                                                                        <  2:429655/150‑1 (MQ=255)
                                                                               cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgcg                                                                        <  2:12242/150‑1 (MQ=255)
                                                                                gTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgcgc                                                                       <  1:561663/150‑1 (MQ=255)
                                                                                  tGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCtt                                                                     >  1:814928/1‑150 (MQ=255)
                                                                                     gcgTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAg                                                                  >  1:878400/1‑150 (MQ=255)
                                                                                       gTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCg                                                                <  2:1101409/150‑1 (MQ=255)
                                                                                          ggTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGAGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATACAGTTGTTTGAAGGCGCGCTTAATCgtgt                                                             >  1:1194234/1‑150 (MQ=255)
                                                                                           gTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc                                                            >  1:416838/1‑150 (MQ=255)
                                                                                              aTTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGc                                                         <  1:624040/150‑1 (MQ=255)
                                                                                               tttCCATTGTTGACTCCTGTATCACTCTACTACCGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt                                                        <  1:194948/150‑1 (MQ=255)
                                                                                                 tCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAAATACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTaa                                                      >  1:250061/1‑150 (MQ=255)
                                                                                                 tCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGAGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTaa                                                      >  1:272088/1‑150 (MQ=255)
                                                                                                  ccATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAAt                                                     <  1:1585734/150‑1 (MQ=255)
                                                                                                   cATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatcatc                                                                                                         >  2:1720806/1‑97 (MQ=255)
                                                                                                   cATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCGGAGTATGCCTTCTTTTATATGCGTAATCAGGGGGCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATg                                                    <  2:244770/150‑1 (MQ=255)
                                                                                                     ttgttgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGcc                                                  <  2:116276/150‑1 (MQ=255)
                                                                                                       gttgACTCCTGTATCACTCTACTACTTTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTg                                                <  2:1037623/150‑1 (MQ=255)
                                                                                                          gACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTa                                             >  2:926186/1‑150 (MQ=255)
                                                                                                          gACTCCTGTATCACTCTACTACGGTGAAAAAAAGAAGGCTGAGTATGGCTTCTTTTATATGCGTAATCAGGGGTGATTTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTaa                                            >  2:922916/1‑150 (MQ=255)
                                                                                                           acacttGTATCAATCTACTACGGTCAAATAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTATTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTaa                                            <  1:1092436/145‑1 (MQ=255)
                                                                                                           aCTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTaa                                            >  2:953007/1‑150 (MQ=255)
                                                                                                            cTCCTGTATCACTCTACTACTGTGAAAAAAAAGAAGGCTGAGTATGGCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAAt                                           <  2:237576/150‑1 (MQ=255)
                                                                                                            cTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAAt                                           <  2:1143238/150‑1 (MQ=255)
                                                                                                                tGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGGCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg                                       <  1:515497/150‑1 (MQ=255)
                                                                                                                tGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg                                       >  1:1025737/1‑150 (MQ=255)
                                                                                                                tGTATCACTCTAATACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTGTATATGCGTAATCAGGGTTCAATTACACATCATCAAGGACAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg                                       <  2:1523170/150‑1 (MQ=255)
                                                                                                                  tATCACTCTACTACGGTGAAAAAAAAGACGGCGGAGTATGCCTTCTTTTATATGCGTAACCAGGGGGCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTTAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt                                     <  2:864408/150‑1 (MQ=255)
                                                                                                                    tCACTCTACAACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTg                                   <  2:672815/150‑1 (MQ=255)
                                                                                                                      aCTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGcc                                 <  2:923954/150‑1 (MQ=255)
                                                                                                                       ctctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGATTACCAGGcattagc                                <  1:860188/150‑7 (MQ=255)
                                                                                                                        tctACTACGGTGAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTc                              <  2:1560682/150‑1 (MQ=255)
                                                                                                                                 gTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTTCCTTCAACGGGTg                      >  1:227196/1‑150 (MQ=255)
                                                                                                                                 cgcatataaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTg                      >  2:1096560/8‑150 (MQ=255)
                                                                                                                                  tGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTAAACGGGTGc                     >  2:863140/1‑150 (MQ=255)
                                                                                                                                   taaaaaaaaGTAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                    <  1:709281/149‑1 (MQ=255)
                                                                                                                                   gAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATTCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                    <  1:202144/150‑1 (MQ=255)
                                                                                                                                   gAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                    <  2:1518405/150‑1 (MQ=255)
                                                                                                                                   gAAAAAAAAGAAGGCTGAGTATCCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                    >  1:786547/1‑150 (MQ=255)
                                                                                                                                    aaaaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATAAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCa                   >  2:1400129/1‑150 (MQ=255)
                                                                                                                                     aaaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCaa                  >  2:2903/1‑150 (MQ=255)
                                                                                                                                      ataaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAac                 >  2:1100606/3‑150 (MQ=255)
                                                                                                                                       taaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCTaca                >  1:298953/2‑150 (MQ=255)
                                                                                                                                        aaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAacac               >  2:1681513/1‑150 (MQ=255)
                                                                                                                                         aaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAATGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACAcc              >  2:41359/1‑150 (MQ=255)
                                                                                                                                         aaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACAcc              >  1:686432/1‑150 (MQ=255)
                                                                                                                                         aaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACAcc              >  1:1011687/1‑150 (MQ=255)
                                                                                                                                             aagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTATTGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTc          <  2:894979/150‑1 (MQ=255)
                                                                                                                                              agGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTcc         <  2:809068/150‑1 (MQ=255)
                                                                                                                                              agGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTcc         >  2:807226/1‑150 (MQ=255)
                                                                                                                                                  tGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGAc     >  1:746880/1‑150 (MQ=255)
                                                                                                                                                     gTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTcc  >  1:597493/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCC  >  NC_000913/2412493‑2412791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: