Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,412,645 T→A 6.3% intergenic (‑51/+32) yfbU ← / ← yfbV UPF0304 family protein/UPF0208 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,412,6450TA 6.3% 390.8 / 7.3 131intergenic (‑51/+32)yfbU/yfbVUPF0304 family protein/UPF0208 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (52/70);  new base A (7/2);  total (59/72)
Fisher's exact test for biased strand distribution p-value = 7.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCCTG  >  NC_000913/2412498‑2412793
                                                                                                                                                   |                                                                                                                                                    
gCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGc                                                                                                                                                    >  2:1510152/1‑150 (MQ=255)
 cAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGcc                                                                                                                                                   <  2:1473882/150‑1 (MQ=255)
 cAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGcc                                                                                                                                                   <  1:1144134/150‑1 (MQ=255)
 cAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGca                                                                                                                                                   >  2:709281/1‑149 (MQ=255)
       ccGGGAACGTTCAGCAGTTGCCGGATCGAGCATAGTCATCCTCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGGCATTTACATTGTTGACTCCGGTATCCCTCTACTACGGGGAAAAAAAAGTCGGCGGAGTATGCCTTCttt                                                                                                                                             <  2:205049/149‑1 (MQ=255)
       aCGGTAACGTTCAGCATTTGCCGGTTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCttt                                                                                                                                             <  2:931271/150‑1 (MQ=255)
        cGGTAACTTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCATACTCAGCCTTCtttt                                                                                                                                            <  1:532598/150‑1 (MQ=255)
        cGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAAAAATACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATACCTTCtttt                                                                                                                                            <  2:1144143/150‑1 (MQ=255)
           ttaCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCATGTACTGATTAGACAAAATAAGTCGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                         <  2:1627976/148‑1 (MQ=255)
           tatagTTCAGCATTTGCCGGATCGAGCATAGTCCTCATCTTGTAATGATTAGACTATATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCATACTCAGCCTTCttttttt                                                                                                                                         <  1:619419/146‑4 (MQ=255)
           tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                         <  1:372949/150‑1 (MQ=255)
           tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                         <  1:1000357/150‑1 (MQ=255)
           tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                         <  1:596596/150‑1 (MQ=255)
           tAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtata                                                                                                                                         >  1:574907/1‑150 (MQ=255)
            aaCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTatat                                                                                                                                        <  1:532498/150‑1 (MQ=255)
              cGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGc                                                                                                                                      >  2:439074/1‑150 (MQ=255)
               gTTCAGCATTTGCCGGATCGAGAATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCg                                                                                                                                     <  2:535739/150‑1 (MQ=255)
                    gCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATc                                                                                                                                <  1:1360418/150‑1 (MQ=255)
                    gCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATc                                                                                                                                >  1:894979/1‑150 (MQ=255)
                           ccGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTc                                                                                                                         <  1:1698115/150‑1 (MQ=255)
                               aTCGAGCATAGTCATCAACTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAAtt                                                                                                                     <  2:558069/150‑1 (MQ=255)
                                tCGAGCATAGTCATCATCTTTTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTa                                                                                                                    <  1:1374874/150‑1 (MQ=255)
                                       tAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTTACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCGTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatca                                                                                                             >  2:523328/1‑150 (MQ=255)
                                       tAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatca                                                                                                             >  1:477697/1‑150 (MQ=255)
                                         gTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatcatc                                                                                                           >  2:1534159/1‑150 (MQ=255)
                                         gTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTATACTACGGTGAAAAAAAAGAAGGCTGAGTATGACTTCTTTTATATGAGTAATCAGGGGTCAATTAAAAatcatc                                                                                                           >  1:1037623/1‑150 (MQ=255)
                                           catcatCTAGTACTGATTAGACAAAATAAGACGTTGCGCGTAGATCATTTCCATTGGTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                                                         <  2:301076/150‑1 (MQ=255)
                                                  ttGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGt                                                                                                  >  1:1346313/1‑150 (MQ=255)
                                                   tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtt                                                                                                 >  2:1437099/1‑150 (MQ=255)
                                                   tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtt                                                                                                 >  2:161589/1‑150 (MQ=255)
                                                   tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtt                                                                                                 >  2:1602048/1‑150 (MQ=255)
                                                    ttACTGATTAGACAAAATAAGACGTTGCGCGTTCGTCATTTCCATTGTTGACTCCTTTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGGCAATTACAAATCATCAAGGAAAGttt                                                                                                <  1:953940/149‑1 (MQ=255)
                                                    gTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGttt                                                                                                <  2:385910/150‑1 (MQ=255)
                                                    gTACTGATTAGACAAAATAAGACGTTGCGCGTTGGGCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGttt                                                                                                <  2:155718/150‑1 (MQ=255)
                                                       cTGATTAGACAAAATAAGACGTTGCGCGTTGGTCCTTTCCATTGTTGACTCCTGTATAACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAATTTTTAt                                                                                             >  1:1523170/1‑150 (MQ=255)
                                                        tGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATc                                                                                            <  1:1166117/150‑1 (MQ=255)
                                                        tGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATc                                                                                            >  1:576428/1‑150 (MQ=255)
                                                         gATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATcc                                                                                           <  2:1441229/150‑1 (MQ=255)
                                                           ttAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAg                                                                                         >  1:1074134/1‑150 (MQ=255)
                                                             aGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgtt                                                                                       >  2:1723837/1‑150 (MQ=255)
                                                              gACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttg                                                                                      <  1:1602048/150‑1 (MQ=255)
                                                              gACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttg                                                                                      >  2:1447719/1‑150 (MQ=255)
                                                                cAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgtt                                                                                    >  2:1325632/1‑150 (MQ=255)
                                                                aaaaaTATGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgtt                                                                                    <  2:1070706/149‑1 (MQ=255)
                                                                 aaaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGttt                                                                                   <  1:1612248/150‑1 (MQ=255)
                                                                  aaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTg                                                                                  <  2:453810/150‑1 (MQ=255)
                                                                  aaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTg                                                                                  <  2:1403262/150‑1 (MQ=255)
                                                                     tAAGACGTTGCGCGTTTGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGTCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                               <  1:852625/150‑1 (MQ=255)
                                                                     tAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                               <  2:243253/150‑1 (MQ=255)
                                                                     tAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                               <  2:184946/150‑1 (MQ=255)
                                                                        gACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGTCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcg                                                                            >  2:1353873/1‑150 (MQ=255)
                                                                         aCGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgc                                                                           >  2:789620/1‑150 (MQ=255)
                                                                          cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                               <  2:828416/145‑1 (MQ=255)
                                                                          cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                               >  1:828416/1‑145 (MQ=255)
                                                                          cGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGgcgcg                                                                          <  2:12242/150‑1 (MQ=255)
                                                                             tGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCtt                                                                       >  1:814928/1‑150 (MQ=255)
                                                                                gcgTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAg                                                                    >  1:878400/1‑150 (MQ=255)
                                                                                  gTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCg                                                                  <  2:1101409/150‑1 (MQ=255)
                                                                                     ggTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGAGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATACAGTTGTTTGAAGGCGCGCTTAATCgtgt                                                               >  1:1194234/1‑150 (MQ=255)
                                                                                      gTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc                                                              >  1:416838/1‑150 (MQ=255)
                                                                                         aTTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGc                                                           <  1:624040/150‑1 (MQ=255)
                                                                                          tttCCATTGTTGACTCCTGTATCACTCTACTACCGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt                                                          <  1:194948/150‑1 (MQ=255)
                                                                                            tCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGAGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTaa                                                        >  1:272088/1‑150 (MQ=255)
                                                                                             ccATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAAt                                                       <  1:1585734/150‑1 (MQ=255)
                                                                                              cATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatcatc                                                                                                           >  2:1720806/1‑97 (MQ=255)
                                                                                              cATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatcatc                                                                                                           <  1:1720806/97‑1 (MQ=255)
                                                                                              cATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCGGAGTATGCCTTCTTTTATATGCGTAATCAGGGGGCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATg                                                      <  2:244770/150‑1 (MQ=255)
                                                                                                ttgttgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGcc                                                    <  2:116276/150‑1 (MQ=255)
                                                                                                     gACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTa                                               >  2:926186/1‑150 (MQ=255)
                                                                                                     gACTCCTGTATCACTCTACTACGGTGAAAAAAAGAAGGCTGAGTATGGCTTCTTTTATATGCGTAATCAGGGGTGATTTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTaa                                              >  2:922916/1‑150 (MQ=255)
                                                                                                      acacttGTATCAATCTACTACGGTCAAATAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTATTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTaa                                              <  1:1092436/145‑1 (MQ=255)
                                                                                                      aCTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTaa                                              >  2:953007/1‑150 (MQ=255)
                                                                                                       cTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAAt                                             <  2:1143238/150‑1 (MQ=255)
                                                                                                           tGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGGCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg                                         <  1:515497/150‑1 (MQ=255)
                                                                                                           tGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg                                         >  1:1025737/1‑150 (MQ=255)
                                                                                                           tGTATCACTCTAATACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTGTATATGCGTAATCAGGGTTCAATTACACATCATCAAGGACAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg                                         <  2:1523170/150‑1 (MQ=255)
                                                                                                             tATCACTCTACTACGGTGAAAAAAAAGACGGCGGAGTATGCCTTCTTTTATATGCGTAACCAGGGGGCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTTAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt                                       <  2:864408/150‑1 (MQ=255)
                                                                                                               tCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCATTTGTTTGAAGGAGCGCTTAAGCGTGTCAGCTAATGCATGTTAATAAGGATTg                                     >  1:841186/1‑150 (MQ=255)
                                                                                                               tCACTCTACAACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTg                                     <  2:672815/150‑1 (MQ=255)
                                                                                                                 aCTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGcc                                   <  2:923954/150‑1 (MQ=255)
                                                                                                                   tctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCtt                                 <  1:1198953/150‑1 (MQ=255)
                                                                                                                         acGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACgg                           <  2:1074134/150‑1 (MQ=255)
                                                                                                                           ggTGAAAAAAAAGAAGTCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTTGTAATCAGGCTTGCCTTCAACGGGt                         <  1:768897/150‑1 (MQ=255)
                                                                                                                            gTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTTCCTTCAACGGGTg                        >  1:227196/1‑150 (MQ=255)
                                                                                                                            cgcatataaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTg                        >  2:1096560/8‑150 (MQ=255)
                                                                                                                             tGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTAAACGGGTGc                       >  2:863140/1‑150 (MQ=255)
                                                                                                                              taaaaaaaaGTAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                      <  1:709281/149‑1 (MQ=255)
                                                                                                                              gAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATTCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                      <  1:202144/150‑1 (MQ=255)
                                                                                                                                aaaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCaa                    >  2:2903/1‑150 (MQ=255)
                                                                                                                                  taaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCTaca                  >  1:298953/2‑150 (MQ=255)
                                                                                                                                   aaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAacac                 <  1:679130/150‑1 (MQ=255)
                                                                                                                                   aaaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAacac                 >  1:298489/1‑150 (MQ=255)
                                                                                                                                    aaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAATGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACAcc                >  2:41359/1‑150 (MQ=255)
                                                                                                                                    aaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCaaaccc                >  1:210343/1‑146 (MQ=255)
                                                                                                                                    aaaGAAGGCATACTCAGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACAcc                >  1:1011687/1‑150 (MQ=255)
                                                                                                                                        aagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTATTGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTc            <  2:894979/150‑1 (MQ=255)
                                                                                                                                         agGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTcc           >  1:430430/1‑150 (MQ=255)
                                                                                                                                         agGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTcc           <  2:809068/150‑1 (MQ=255)
                                                                                                                                         agGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTcc           >  2:807226/1‑150 (MQ=255)
                                                                                                                                             tGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGAc       >  1:746880/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCCTG  >  NC_000913/2412498‑2412793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: