Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,417,340 A→T 6.6% D87V (GAT→GTT)  yfcC → putative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,417,3400AT 6.6% 351.7 / 11.3 122D87V (GAT→GTT) yfcCputative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein
Reads supporting (aligned to +/- strand):  ref base A (50/63);  new base T (4/4);  total (55/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.25e-01

Only 100 of 124 total aligned reads displayed.

GGTGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATCC  >  NC_000913/2417191‑2417489
                                                                                                                                                     |                                                                                                                                                     
ggTGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGa                                                                                                                                                       >  1:1554933/1‑150 (MQ=255)
  tGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATg                                                                                                                                                     <  2:737813/150‑1 (MQ=255)
   ggggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGa                                                                                                                                                    <  2:1291614/150‑1 (MQ=255)
   ggggATGTTTGACAGGCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACTCGCAAAGTCGTAGATCCACACTCATTTCGCTTTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGa                                                                                                                                                    <  2:1685512/150‑1 (MQ=255)
    gggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCAACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGaa                                                                                                                                                   >  1:1319289/1‑150 (MQ=255)
     ggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCTTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAAc                                                                                                                                                  <  2:264454/150‑1 (MQ=255)
     ggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAAc                                                                                                                                                  >  2:1428802/1‑150 (MQ=255)
        tGTTTGACAGACAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGcc                                                                                                                                               <  2:496794/150‑1 (MQ=255)
         gTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGccc                                                                                                                                              <  2:931979/150‑1 (MQ=255)
             gACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGc                                                                                                                                          >  1:70969/1‑150 (MQ=255)
              aCAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTAGCACCGCGTACAGCTGTTCACGACGGGCGTTCATCGCCCgtcgt                                                                                                                                         <  1:271308/150‑5 (MQ=255)
               cAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATACACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCt                                                                                                                                        >  1:1309794/1‑150 (MQ=255)
                aGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGCTCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGATCACGACGGGCGAGGAACGCCCGGGCCtg                                                                                                                                       >  1:1616549/1‑150 (MQ=255)
                aGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCAACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGTTCATCGCCCgtcgtga                                                                                                                                       <  2:338409/150‑7 (MQ=255)
                   cAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtgatg                                                                                                                                    <  1:1635956/150‑1 (MQ=255)
                   cAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCGGTTCACGACGGGCGATGAACGCCCGGGCCtgatg                                                                                                                                    <  2:1499009/150‑1 (MQ=255)
                    aGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtgatga                                                                                                                                   >  2:1411073/1‑150 (MQ=255)
                    aGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtgatga                                                                                                                                   <  1:1680722/150‑1 (MQ=255)
                     ggAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCTATGAACGCCCGGGCCTGATGaa                                                                                                                                  >  2:1650824/1‑150 (MQ=255)
                      gAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtga                                                                                                                                      >  1:897256/1‑145 (MQ=255)
                      gAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtga                                                                                                                                      <  2:897256/145‑1 (MQ=255)
                      gAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAAc                                                                                                                                 >  1:1148656/1‑150 (MQ=255)
                       aaGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACt                                                                                                                                <  1:954838/150‑1 (MQ=255)
                           gCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTccc                                                                                                                            >  1:1687075/1‑150 (MQ=255)
                           gCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTccc                                                                                                                            >  1:1469902/1‑150 (MQ=255)
                           gCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTccc                                                                                                                            >  1:1212074/1‑150 (MQ=255)
                            cAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCg                                                                                                                           <  1:847947/150‑1 (MQ=255)
                              ttATCAGGTTGATGTTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGtt                                                                                                                         <  2:524719/149‑1 (MQ=255)
                              gTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGtt                                                                                                                         >  1:874204/1‑150 (MQ=255)
                              gTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGtt                                                                                                                         >  1:1237425/1‑150 (MQ=255)
                               tATCAGGTTGATGGTCAAACAAAAACACACAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGttt                                                                                                                        <  2:1496742/150‑1 (MQ=255)
                                aTCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGCACGCCCGGGCCTGATGAACTTCCCGTTTg                                                                                                                       >  2:601086/1‑150 (MQ=255)
                                  cAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGaa                                                                                                                     <  2:1481500/150‑1 (MQ=255)
                                   aGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAg                                                                                                                    <  2:776752/150‑1 (MQ=255)
                                    ggTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAgg                                                                                                                   <  1:447475/150‑1 (MQ=255)
                                       tGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCTCACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGAtt                                                                                                                <  2:751433/150‑1 (MQ=255)
                                       tGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCACGGGCCTGATGAACTTCCCGTTTGAAGGAtt                                                                                                                <  2:126172/150‑1 (MQ=255)
                                         aTGGTCAAACAAAAACACGCAAAGGCGTAGATCCACACACATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGGTCACGACTGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTaa                                                                                                              <  1:269522/150‑1 (MQ=255)
                                           ggTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCTCGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAAcc                                                                                                            <  1:309501/150‑1 (MQ=255)
                                           ggTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAAcc                                                                                                            >  2:283555/1‑150 (MQ=255)
                                             tCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTc                                                                                                          >  2:1528639/1‑150 (MQ=255)
                                             tCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTc                                                                                                          <  1:1441862/150‑1 (MQ=255)
                                                  caaaAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGAt                                                                                                     <  1:1194998/150‑1 (MQ=255)
                                                   aaaaaCACGCAAAGTCGTAGATCCACACTCATATCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATc                                                                                                    <  2:1100444/150‑1 (MQ=255)
                                                     aaaCACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGAGGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGa                                                                                                  <  2:874204/150‑1 (MQ=255)
                                                       acacGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGaaa                                                                                                <  1:1428802/150‑1 (MQ=255)
                                                          cGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATAc                                                                                             <  1:1527413/150‑1 (MQ=255)
                                                              aaGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGa                                                                                         >  1:1595739/1‑150 (MQ=255)
                                                                gTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAATCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACa                                                                                       <  1:1027173/150‑1 (MQ=255)
                                                                gTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTGCCGTTTGAAGGATTAACCTCAGGATCGTAATTCGGGACa                                                                                       >  1:1096144/1‑150 (MQ=255)
                                                                gTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTa                                                                                                               >  2:1219351/1‑126 (MQ=255)
                                                                  cGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGc                                                                                     >  1:457135/1‑150 (MQ=255)
                                                                     tgATCCTCACTCATTTCTCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTTTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGt                                                                                  <  1:1066690/149‑1 (MQ=255)
                                                                        tgcacaCTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCGGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTgg                                                                               <  1:29152/148‑1 (MQ=255)
                                                                             acTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatca                                                                          >  1:1246936/1‑150 (MQ=255)
                                                                              cTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGCTGCACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcat                                                                         >  2:138331/1‑150 (MQ=255)
                                                                              cTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGCACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcat                                                                         >  1:584559/1‑150 (MQ=255)
                                                                              cTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcat                                                                         >  1:1550684/1‑150 (MQ=255)
                                                                                  tttCGCATTCTGACTAACGAAGCAAGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGACCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGCTCGAAATACGGGACCGCCGTTGGcatcatcagg                                                                     >  2:46046/1‑148 (MQ=255)
                                                                                    tCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGtt                                                                   <  1:104508/150‑1 (MQ=255)
                                                                                    tCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGtt                                                                   <  2:913904/150‑1 (MQ=255)
                                                                                     cGCATTCTGCCTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGttt                                                                  <  2:118381/150‑1 (MQ=255)
                                                                                     cGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGttt                                                                  >  1:419288/1‑150 (MQ=255)
                                                                                        aTTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGACCTTCCCTTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATg                                                               <  1:245376/150‑1 (MQ=255)
                                                                                        aTTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATg                                                               >  1:1393217/1‑150 (MQ=255)
                                                                                         ttCTGACTACCGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGc                                                              <  1:1061439/150‑1 (MQ=255)
                                                                                         ttCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCACAGGATCGAAATAAGGGACAGCCCTTGGCAGCATCATGTTTATGc                                                              >  1:72589/1‑150 (MQ=255)
                                                                                            tGACTACCGAAGCAGGCGAAACTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTgg                                                           <  1:355566/150‑1 (MQ=255)
                                                                                            tGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTgg                                                           >  2:1026299/1‑150 (MQ=255)
                                                                                             gACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGt                                                          <  1:87215/150‑1 (MQ=255)
                                                                                               cTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGa                                                        >  1:1202198/1‑150 (MQ=255)
                                                                                               cTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGa                                                        <  2:594418/150‑1 (MQ=255)
                                                                                                     aaGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcg                                                  >  1:613593/1‑150 (MQ=255)
                                                                                                      aGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcgg                                                 <  1:128848/150‑1 (MQ=255)
                                                                                                        cAGGCGAACCTGAGTATCTCCGCGTACAGCTGTTCAAGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGgcggcg                                               <  2:180016/150‑1 (MQ=255)
                                                                                                        cAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGTGACAGCCGTTGGCGTCATCATGTTTATGCTGGTGATTGgcggcg                                               >  2:290477/1‑150 (MQ=255)
                                                                                                          ggCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACGTCAGGATCGAAATACGGGCCAGCCGTGGGCATCCTCATGTTTATGCTGGTGATTGGCGGcgcg                                             >  1:68423/1‑150 (MQ=255)
                                                                                                          ggCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGcgcg                                             >  2:471618/1‑150 (MQ=255)
                                                                                                                  tGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCAt                                     >  1:249/1‑150 (MQ=255)
                                                                                                                      tATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATtgtg                                 >  2:1267912/1‑150 (MQ=255)
                                                                                                                       aTCACCGCGGACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGa                                <  1:603404/150‑1 (MQ=255)
                                                                                                                        tCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGAt                               >  1:1006585/1‑150 (MQ=255)
                                                                                                                         cACCGCGTACAGCTGGTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg                              <  1:1369533/150‑1 (MQ=255)
                                                                                                                            cgcgTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGt                           >  1:1407048/1‑150 (MQ=255)
                                                                                                                               ttACAGCTGTTCACGACGGGCGATGAACTCCCGGGACTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACa                        <  2:1105813/149‑1 (MQ=255)
                                                                                                                                 aCAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAgg                      <  2:767977/150‑1 (MQ=255)
                                                                                                                                       gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAt                >  1:1095780/1‑150 (MQ=255)
                                                                                                                                        ttCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAtt               <  2:1687075/150‑1 (MQ=255)
                                                                                                                                        ttCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAtt               <  2:613593/150‑1 (MQ=255)
                                                                                                                                         tCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTg              <  2:1060086/150‑1 (MQ=255)
                                                                                                                                          cACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGa             >  1:104267/1‑150 (MQ=255)
                                                                                                                                           ggcccgGGCGTTCATCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACCGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTTCAGGAACCATTGAt            >  1:1064111/5‑150 (MQ=255)
                                                                                                                                            cgacgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATa           >  2:1269946/1‑150 (MQ=255)
                                                                                                                                             cccgGGCGTTCATCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATaa          >  2:59421/3‑150 (MQ=255)
                                                                                                                                             cccgGGCGTTCATCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATaa          >  1:984388/3‑150 (MQ=255)
                                                                                                                                               cgGGCGATGCACGCCCGTGCCTGATGAACTTCCCGTTTGAAGGATTACCCTCAGGATCGAAATACGGGACAGCAGTTGGCCTCATCATGTTTATGATGGTGATTTGCGGCGCGTATGGCATTTTGATGCGTACATGAACCATTGATAAcc        >  2:1639331/1‑149 (MQ=255)
                                                                                                                                                   cGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTAt    >  1:143492/1‑150 (MQ=255)
                                                                                                                                                    gATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATc   <  1:1572295/150‑1 (MQ=255)
                                                                                                                                                    gATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATc   <  2:1393217/150‑1 (MQ=255)
                                                                                                                                                     aTGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATcc  >  2:1004584/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATCC  >  NC_000913/2417191‑2417489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: