Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,417,342 G→C 6.6% E88Q (GAA→CAA) ‡ yfcC → putative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,417,3420GC 6.6% 368.7 / 15.0 121E88Q (GAA→CAA) ‡yfcCputative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein
Reads supporting (aligned to +/- strand):  ref base G (52/60);  new base C (4/4);  total (56/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-02

Only 100 of 122 total aligned reads displayed.

TGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATCC  >  NC_000913/2417193‑2417489
                                                                                                                                                     |                                                                                                                                                   
tGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATg                                                                                                                                                     <  2:737813/150‑1 (MQ=255)
 ggggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGa                                                                                                                                                    <  2:1291614/150‑1 (MQ=255)
 ggggATGTTTGACAGGCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACTCGCAAAGTCGTAGATCCACACTCATTTCGCTTTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGa                                                                                                                                                    <  2:1685512/150‑1 (MQ=255)
  gggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCAACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGaa                                                                                                                                                   >  1:1319289/1‑150 (MQ=255)
   ggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCTTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAAc                                                                                                                                                  <  2:264454/150‑1 (MQ=255)
   ggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAAc                                                                                                                                                  >  2:1428802/1‑150 (MQ=255)
      tGTTTGACAGACAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGcc                                                                                                                                               <  2:496794/150‑1 (MQ=255)
       gTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGccc                                                                                                                                              <  2:931979/150‑1 (MQ=255)
           gACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGc                                                                                                                                          >  1:70969/1‑150 (MQ=255)
             cAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAACGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGTTCATCGCCCgtcgtg                                                                                                                                        <  2:155866/150‑6 (MQ=255)
             cAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATACACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCt                                                                                                                                        >  1:1309794/1‑150 (MQ=255)
              aGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGCTCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGATCACGACGGGCGAGGAACGCCCGGGCCtg                                                                                                                                       >  1:1616549/1‑150 (MQ=255)
              aGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCAACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGTTCATCGCCCgtcgtga                                                                                                                                       <  2:338409/150‑7 (MQ=255)
                 cAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtgatg                                                                                                                                    <  1:1635956/150‑1 (MQ=255)
                  aGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtgatga                                                                                                                                   >  2:1411073/1‑150 (MQ=255)
                   ggAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCTATGAACGCCCGGGCCTGATGaa                                                                                                                                  >  2:1650824/1‑150 (MQ=255)
                    gAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtga                                                                                                                                      >  1:897256/1‑145 (MQ=255)
                    gAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtga                                                                                                                                      <  2:897256/145‑1 (MQ=255)
                     aaGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACt                                                                                                                                <  1:954838/150‑1 (MQ=255)
                         gCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTccc                                                                                                                            >  1:1212074/1‑150 (MQ=255)
                         gCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTccc                                                                                                                            >  1:1687075/1‑150 (MQ=255)
                         gCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTccc                                                                                                                            >  1:1469902/1‑150 (MQ=255)
                          cAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCg                                                                                                                           <  1:847947/150‑1 (MQ=255)
                            gTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGtt                                                                                                                         >  1:874204/1‑150 (MQ=255)
                             tATCAGGTTGATGGTCAAACAAAAACACACAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGttt                                                                                                                        <  2:1496742/150‑1 (MQ=255)
                              aTCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGCACGCCCGGGCCTGATGAACTTCCCGTTTg                                                                                                                       >  2:601086/1‑150 (MQ=255)
                                cAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGaa                                                                                                                     <  2:1481500/150‑1 (MQ=255)
                                 aGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAg                                                                                                                    <  2:776752/150‑1 (MQ=255)
                                 aGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAg                                                                                                                    <  2:228120/150‑1 (MQ=255)
                                  ggTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAgg                                                                                                                   <  1:447475/150‑1 (MQ=255)
                                   gTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAggt                                                                                                                  >  1:1708994/1‑149 (MQ=255)
                                     tGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCTCACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGAtt                                                                                                                <  2:751433/150‑1 (MQ=255)
                                       aTGGTCAAACAAAAACACGCAAAGGCGTAGATCCACACACATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGGTCACGACTGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTaa                                                                                                              <  1:269522/150‑1 (MQ=255)
                                         ggTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCTCGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAAcc                                                                                                            <  1:309501/150‑1 (MQ=255)
                                         ggTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAAcc                                                                                                            >  2:283555/1‑150 (MQ=255)
                                         ggTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAAcc                                                                                                            >  1:1314771/1‑150 (MQ=255)
                                           tCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTc                                                                                                          >  2:1528639/1‑150 (MQ=255)
                                           tCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTc                                                                                                          <  1:1441862/150‑1 (MQ=255)
                                                caaaAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGAt                                                                                                     <  1:1194998/150‑1 (MQ=255)
                                                  ataaCAGGCTAAGTCGTAGATCCACACTCATTTCGCATTCTGACAAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCg                                                                                                   <  2:1309794/148‑1 (MQ=255)
                                                   aaaCACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGAGGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGa                                                                                                  <  2:874204/150‑1 (MQ=255)
                                                     acacGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGaaa                                                                                                <  1:1428802/150‑1 (MQ=255)
                                                        cGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATAc                                                                                             <  1:1527413/150‑1 (MQ=255)
                                                          cAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCTATTACAGCGGTTCACGACGGGCGATTAACGCCCGGGCCTGATGAACTTCCCGTTTGATGGATTAACCTCTGGATCGAAATACgg                                                                                           <  1:1111888/150‑1 (MQ=255)
                                                            aaGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGa                                                                                         >  1:1595739/1‑150 (MQ=255)
                                                            aaGTCGTAGATCCACACTCATTTCGCAATCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATTAACTTCCCGTTTGAAGGATTAACCTCAGGATCGACAAACGGGa                                                                                         >  1:282007/1‑150 (MQ=255)
                                                              gTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAATCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACa                                                                                       <  1:1027173/150‑1 (MQ=255)
                                                              gTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTGCCGTTTGAAGGATTAACCTCAGGATCGTAATTCGGGACa                                                                                       >  1:1096144/1‑150 (MQ=255)
                                                              gTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTa                                                                                                               <  1:1219351/126‑1 (MQ=255)
                                                              gTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTa                                                                                                               >  2:1219351/1‑126 (MQ=255)
                                                                cGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGc                                                                                     >  1:457135/1‑150 (MQ=255)
                                                                   tgATCCTCACTCATTTCTCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTTTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGt                                                                                  <  1:1066690/149‑1 (MQ=255)
                                                                      tgcacaCTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCGGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTgg                                                                               <  1:29152/148‑1 (MQ=255)
                                                                           acTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatca                                                                          >  1:1246936/1‑150 (MQ=255)
                                                                            cTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGCTGCACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcat                                                                         >  2:138331/1‑150 (MQ=255)
                                                                            cTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGCACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcat                                                                         >  1:584559/1‑150 (MQ=255)
                                                                            cTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcat                                                                         >  1:1550684/1‑150 (MQ=255)
                                                                              cATTTCGCATTCTGACTAACGAAGCACGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGACCGCCCGGGCCTGATGAACTTCCCGTATGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGtcatcatca                                                                       >  1:1492348/1‑150 (MQ=255)
                                                                                tttCGCATTCTGACTAACGAAGCAAGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGACCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGCTCGAAATACGGGACCGCCGTTGGcatcatcagg                                                                     >  2:46046/1‑148 (MQ=255)
                                                                                  tCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGtt                                                                   <  2:913904/150‑1 (MQ=255)
                                                                                  tCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGtt                                                                   <  1:104508/150‑1 (MQ=255)
                                                                                   cGCATTCTGCCTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGttt                                                                  <  2:118381/150‑1 (MQ=255)
                                                                                      aTTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGACCTTCCCTTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATg                                                               <  1:245376/150‑1 (MQ=255)
                                                                                      aTTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATg                                                               >  1:1393217/1‑150 (MQ=255)
                                                                                       ttCTGACTACCGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGc                                                              <  1:1061439/150‑1 (MQ=255)
                                                                                       ttCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCACAGGATCGAAATAAGGGACAGCCCTTGGCAGCATCATGTTTATGc                                                              >  1:72589/1‑150 (MQ=255)
                                                                                          tGACTACCGAAGCAGGCGAAACTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTgg                                                           <  1:355566/150‑1 (MQ=255)
                                                                                          tGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTgg                                                           >  2:1026299/1‑150 (MQ=255)
                                                                                           gACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGt                                                          <  1:87215/150‑1 (MQ=255)
                                                                                             cTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGa                                                        <  2:594418/150‑1 (MQ=255)
                                                                                             cTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGa                                                        >  1:1202198/1‑150 (MQ=255)
                                                                                                   aaGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcg                                                  >  1:613593/1‑150 (MQ=255)
                                                                                                    aGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcgg                                                 <  1:128848/150‑1 (MQ=255)
                                                                                                      cAGGCGAACCTGAGTATCTCCGCGTACAGCTGTTCAAGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGgcggcg                                               <  2:180016/150‑1 (MQ=255)
                                                                                                       aGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGgcgc                                              >  1:443909/1‑150 (MQ=255)
                                                                                                        ggCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACGTCAGGATCGAAATACGGGCCAGCCGTGGGCATCCTCATGTTTATGCTGGTGATTGGCGGcgcg                                             >  1:68423/1‑150 (MQ=255)
                                                                                                        ggCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGcgcg                                             >  2:471618/1‑150 (MQ=255)
                                                                                                           caaCCTGAGTATCACCGCGTACAGCTGGTCACGCCGGGCGATGAACGCCCGGGCCTGATGAACTACCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGttt                                          <  2:1096144/149‑1 (MQ=255)
                                                                                                                tGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCAt                                     >  1:249/1‑150 (MQ=255)
                                                                                                                    tATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATtgtg                                 >  2:1267912/1‑150 (MQ=255)
                                                                                                                     aTCACCGCGGACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGa                                <  1:603404/150‑1 (MQ=255)
                                                                                                                      tCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGAt                               >  1:1006585/1‑150 (MQ=255)
                                                                                                                       cACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg                              <  2:70969/150‑1 (MQ=255)
                                                                                                                         ccGCGAACAGGTGTTCACGACTGGCGATGAACGCCCGGGCCTGATGATCTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCg                            <  2:431973/150‑1 (MQ=255)
                                                                                                                          cgcgTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGt                           >  1:1407048/1‑150 (MQ=255)
                                                                                                                             ttACAGCTGTTCACGACGGGCGATGAACTCCCGGGACTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACa                        <  2:1105813/149‑1 (MQ=255)
                                                                                                                              tACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAg                       <  1:1411073/150‑1 (MQ=255)
                                                                                                                               aCAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAgg                      <  2:767977/150‑1 (MQ=255)
                                                                                                                                   cTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAAcc                  <  2:576992/150‑1 (MQ=255)
                                                                                                                                     gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAt                >  1:1095780/1‑150 (MQ=255)
                                                                                                                                       tCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTg              <  2:1060086/150‑1 (MQ=255)
                                                                                                                                        cACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGa             >  1:104267/1‑150 (MQ=255)
                                                                                                                                         ggcccgGGCGTTCATCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACCGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTTCAGGAACCATTGAt            >  1:1064111/5‑150 (MQ=255)
                                                                                                                                          cgacgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATa           >  2:1269946/1‑150 (MQ=255)
                                                                                                                                           cccgGGCGTTCATCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATaa          >  2:59421/3‑150 (MQ=255)
                                                                                                                                           cccgGGCGTTCATCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATaa          >  1:984388/3‑150 (MQ=255)
                                                                                                                                             cgGGCGATGCACGCCCGTGCCTGATGAACTTCCCGTTTGAAGGATTACCCTCAGGATCGAAATACGGGACAGCAGTTGGCCTCATCATGTTTATGATGGTGATTTGCGGCGCGTATGGCATTTTGATGCGTACATGAACCATTGATAAcc        >  2:1639331/1‑149 (MQ=255)
                                                                                                                                               ggCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGt      >  1:1603746/1‑150 (MQ=255)
                                                                                                                                                 cGATGAAAGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTAt    <  2:1246936/150‑1 (MQ=255)
                                                                                                                                                   aTGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATcc  >  2:1004584/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATCC  >  NC_000913/2417193‑2417489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: