Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,430,937 C→A 5.5% intergenic (‑174/+85) cvpA ← / ← dedD colicin V production protein/membrane‑anchored periplasmic protein involved in septation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,9370CA 5.5% 286.8 / 5.7 99intergenic (‑174/+85)cvpA/dedDcolicin V production protein/membrane‑anchored periplasmic protein involved in septation
Reads supporting (aligned to +/- strand):  ref base C (38/55);  new base A (2/4);  total (40/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

Only 100 of 102 total aligned reads displayed.

GTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGC  >  NC_000913/2430788‑2431085
                                                                                                                                                     |                                                                                                                                                      
gTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTAGCGGCATCGGGCAATGCGTGTCGGATgc                                                                                                                                                        >  1:888353/1‑150 (MQ=255)
  cATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcgg                                                                                                                                                      >  1:405524/1‑150 (MQ=255)
      cTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCAGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTa                                                                                                                                                  >  1:778398/1‑150 (MQ=255)
            ggtgcgcATTCTAACATTAAAAGAAAACGTTTGCGTAGGGATTGACTTCCCGCGCATCATTAAAATTGGCGCTGTAAAAATATTCAACGCCATCGACTTATTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGATGCGGCGTAAACGcc                                                                                                                                            <  1:1231694/147‑1 (MQ=255)
            ggggCTCATTCTAACAGAAAAAGAAAACGTTTGCTTAGGGAATTCCTTCGCTCTCATCATTAAATATGGCGCTGATAATATATTCAACGCCATCGACTTTTTATGACTTTGCGGCATCGGGCAATGCGTGTCCGATGCGGCGTAAACGcc                                                                                                                                            <  1:99844/150‑1 (MQ=255)
            ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCTATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                                                                                            <  2:133546/150‑1 (MQ=255)
            ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                                                                                            <  1:750954/150‑1 (MQ=255)
             gggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGAGGCGGCGTAAACGCCt                                                                                                                                           <  2:905017/150‑1 (MQ=255)
              ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt                                                                                                                                          <  1:1351899/150‑1 (MQ=255)
                 gcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGTGTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                       >  2:901585/1‑150 (MQ=255)
                 gcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                       <  2:1399639/150‑1 (MQ=255)
                  cATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                      >  2:750404/1‑150 (MQ=255)
                      cTAACAGTAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                  <  2:74636/150‑1 (MQ=255)
                      cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                  <  1:1304754/150‑1 (MQ=255)
                      cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                  <  2:1572550/150‑1 (MQ=255)
                       tAACAGAAAAAGAAAACTTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcac                                                                                                                                 <  2:1197851/150‑3 (MQ=255)
                       tAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCt                                                                                                                                 >  1:1184427/1‑150 (MQ=255)
                        aaCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa                                                                                                                                >  2:328236/1‑150 (MQ=255)
                        aaCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacg                                                                                                                                <  1:1002886/150‑4 (MQ=255)
                        aaCAGAAAAACAAAACGTTTGAGTAGGTAATTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACATTATATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacg                                                                                                                                <  1:265401/150‑4 (MQ=255)
                           agaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                                             <  1:1029675/150‑1 (MQ=255)
                            gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                            >  2:1230577/1‑150 (MQ=255)
                            gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGAGTAAACGCCTTATCCGACCTACGGt                                                                                                                            >  2:1474037/1‑150 (MQ=255)
                            gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacgcatt                                                                                                                            <  2:1279141/150‑8 (MQ=38)
                              aaaagcaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCCGGTTc                                                                                                                          >  2:1590763/1‑150 (MQ=255)
                              aaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTc                                                                                                                          <  1:1621717/150‑1 (MQ=255)
                              aaaagaaaaCGTTTGCGTAGGGATTTCCTTACCGCGCATCAATAAAAATGGAGCTGAAAAAATAATAAACGCAATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTTTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTc                                                                                                                          <  2:1556281/150‑1 (MQ=39)
                                 agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGTGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                       >  2:1096529/1‑150 (MQ=255)
                                 agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                       >  1:706045/1‑150 (MQ=255)
                                 agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                       >  1:699805/1‑150 (MQ=255)
                                 agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCATTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                       >  1:515314/1‑150 (MQ=255)
                                 agaaaaCGATTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCACCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGAAGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                       <  2:342581/150‑1 (MQ=255)
                                  gaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTATGGTTCTAcc                                                                                                                      <  2:1706540/150‑1 (MQ=255)
                                   aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                                                                                     <  1:745584/150‑1 (MQ=255)
                                   aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                                                                                     <  1:1601801/150‑1 (MQ=255)
                                    aaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATCGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAcccc                                                                                                                    <  1:792130/150‑1 (MQ=255)
                                      aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGTGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTg                                                                                                                  <  1:901585/150‑1 (MQ=255)
                                      aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTg                                                                                                                  <  1:809769/150‑1 (MQ=255)
                                        gTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGCCTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑g                                                                                                              >  2:350133/1‑150 (MQ=255)
                                             cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGTATGAGTCGTAAACGCCATATCCGAACTACGGTTCTACCCCTGC‑‑GTAGGc                                                                                                         >  2:36476/1‑150 (MQ=255)
                                              gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                  <  2:1568471/126‑1 (MQ=255)
                                              gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                  >  1:1568471/1‑126 (MQ=255)
                                              gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGcc                                                                                                        <  1:54982/150‑1 (MQ=255)
                                                aGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTg                                                                                                      <  2:1201834/150‑1 (MQ=255)
                                                aGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTg                                                                                                      <  2:879035/150‑1 (MQ=255)
                                                 gggATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTTCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGa                                                                                                     >  2:1641401/1‑150 (MQ=255)
                                                 gggATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGa                                                                                                     >  2:810461/1‑150 (MQ=255)
                                                     tttCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGTCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAg                                                                                                 <  2:848088/150‑1 (MQ=255)
                                                      ttccttccCGCGCATCAATAAAAATGGCGCGGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGa                                                                                                <  1:1279718/150‑1 (MQ=255)
                                                       tccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGAc                                                                                               <  1:990686/150‑1 (MQ=255)
                                                        ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGAcg                                                                                              >  2:1324402/1‑150 (MQ=255)
                                                           tccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACgcgc                                                                                           <  2:38540/150‑1 (MQ=255)
                                                                   aTCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCCGATAAGACGCGCCAGCGTCg                                                                                   <  1:1374089/150‑1 (MQ=255)
                                                                    tCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGc                                                                                  <  2:326411/150‑1 (MQ=255)
                                                                      aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCAt                                                                                >  2:564171/1‑150 (MQ=255)
                                                                       aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCATTATCCGACCTACGGTTCTACCCCTGC‑‑GTCGGCCTGATAATACGCGCCAGCGTCGCATc                                                                               >  2:121087/1‑150 (MQ=255)
                                                                          aaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg                                                                            <  1:1004132/150‑1 (MQ=255)
                                                                           cgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                           >  1:311983/3‑150 (MQ=37)
                                                                            gaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCAACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                          >  1:1432264/2‑150 (MQ=37)
                                                                            gaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGAGGCATCGTGCAATGCGTATCGGATGCGTCGTAAAAGCCAGAACAGACATACGTTTATACCCCTGC‑‑GTAGGCCTGATAATAAGACCCATCGTCGCATCATGCa                                                                          >  2:903665/2‑150 (MQ=255)
                                                                            aaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                          <  2:1447676/150‑1 (MQ=255)
                                                                              tGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTTTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTTAACGCCTTATCCGACCTACGGTTCTACCCCGGC‑‑GTAGGCCTGCTAAGACGCGCCAGCGTCGCATCAGGCAAg                                                                        >  1:1529891/1‑150 (MQ=255)
                                                                                gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc                                                                      <  2:560763/150‑1 (MQ=255)
                                                                                      aaaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                <  2:1184427/150‑1 (MQ=255)
                                                                                      aaaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                <  1:972680/150‑1 (MQ=255)
                                                                                       aaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa                                                               <  1:409364/150‑1 (MQ=255)
                                                                                            tatTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCg                                                          >  1:189465/1‑150 (MQ=255)
                                                                                             atTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCgg                                                         >  2:836263/1‑150 (MQ=255)
                                                                                              ttCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGc                                                        <  2:189465/150‑1 (MQ=255)
                                                                                               tCAACGCCATCGACTTATTATGCCTTTGCTGCATCGGGCAATGCGTGTCGGATGCGGCGTAACCGCCTTATCAGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCACATCAGGCAAGACCGTATTAATTCGGCg                                                       <  1:845150/150‑1 (MQ=39)
                                                                                                    gCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCTTATTAATTCGGCGtatat                                                  >  2:31740/1‑149 (MQ=255)
                                                                                                    gCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTATTTCGGCGTATAg                                                  >  1:848905/1‑150 (MQ=255)
                                                                                                      cATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGAAAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGcc                                                <  1:750404/150‑1 (MQ=255)
                                                                                                        tCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCa                                              >  2:427646/1‑150 (MQ=255)
                                                                                                             tttttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGACAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTAcc                                         <  1:329747/150‑1 (MQ=255)
                                                                                                              ttttATGCCTTTGCGGCATCGGGAAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCTACCTACGGTTCTACCCCTGC‑‑TTAGGCCTTATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCa                                        <  2:1269547/150‑1 (MQ=255)
                                                                                                               tttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCAc                                       <  2:1286373/150‑1 (MQ=255)
                                                                                                                   tGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCa                                   >  2:633037/1‑150 (MQ=255)
                                                                                                                       tttGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTa                               >  1:98355/1‑150 (MQ=255)
                                                                                                                       ttggCGGCATCGGGCAAGGCGTGTGGTAGGCGGCTAAACCGCCTTACCCGACCTACGGTTCTCCCCCGGC‑‑GTCGGCCTCAAAAGACGCCCCCGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTa                               <  1:1572456/147‑1 (MQ=255)
                                                                                                                         tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTACACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                             >  1:1083579/1‑150 (MQ=255)
                                                                                                                         tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                             <  2:904990/150‑1 (MQ=255)
                                                                                                                         tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                             <  1:353923/150‑1 (MQ=255)
                                                                                                                          gCGGCTTCGGGCATTGCGTGTGGGATGCGGCGTAAACGCCTTATCCGACCTACTGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCTTCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc                            <  1:1474037/150‑1 (MQ=38)
                                                                                                                          gCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc                            >  1:844241/1‑150 (MQ=255)
                                                                                                                            ggCATCGGGCAATGCGTGGCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGCTACCCCTGC‑‑GTAGGCCTGATAAGACGAGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCa                          <  1:306169/150‑1 (MQ=255)
                                                                                                                                 cGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAg                     <  1:1240513/150‑1 (MQ=255)
                                                                                                                                  gggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGt                    >  1:1670726/1‑150 (MQ=255)
                                                                                                                                   ggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCCCTTAAGCCAGAAAGtt                   >  1:49592/1‑150 (MQ=255)
                                                                                                                                    gCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTg                  <  1:550335/150‑1 (MQ=255)
                                                                                                                                    gCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAAGGCTATACACCAGC‑‑GTAGTCCTGATAAGACGCGCCAGCGACGCATCAGTCAAGAACGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTg                  <  1:121087/150‑1 (MQ=38)
                                                                                                                                     cAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGc                 <  2:1122763/150‑1 (MQ=255)
                                                                                                                                      aaTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCt                <  1:564171/150‑1 (MQ=255)
                                                                                                                                          cGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGATAGTTGCTTCaa            >  1:825763/1‑150 (MQ=255)
                                                                                                                                           gtgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGTCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAAc           <  2:34621/150‑1 (MQ=255)
                                                                                                                                            tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt          <  2:262989/150‑1 (MQ=255)
                                                                                                                                            ggtCGGATAAGGCGTTTACGCCGCATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt          >  2:931663/2‑150 (MQ=39)
                                                                                                                                               cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGGATTAATTCGGAGTATAGCCCATTTCCACGCCACTTAAGCCAGCAAGTTGCTTCAACTccc       >  2:156496/1‑148 (MQ=255)
                                                                                                                                                 gATGCGGCGCGAACGCCTTATCCGGCCTACGGTTCGGCACAAACCTGTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCAc       >  1:183212/1‑150 (MQ=18)
                                                                                                                                                    gcggcgTAAACGCCTTATCCTACCTACGGTTCTACCCCTGC‑‑GTAGGACTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGc  <  2:515314/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGC  >  NC_000913/2430788‑2431085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: