Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,430,943 A→T 7.6% intergenic (‑180/+79) cvpA ← / ← dedD colicin V production protein/membrane‑anchored periplasmic protein involved in septation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,9430AT 7.6% 293.3 / 9.3 104intergenic (‑180/+79)cvpA/dedDcolicin V production protein/membrane‑anchored periplasmic protein involved in septation
Reads supporting (aligned to +/- strand):  ref base A (38/57);  new base T (4/4);  total (43/61)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTT  >  NC_000913/2430794‑2431092
                                                                                                                                                     |                                                                                                                                                       
cTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCAGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTa                                                                                                                                                         >  1:778398/1‑150 (MQ=255)
      ggtgcgcATTCTAACATTAAAAGAAAACGTTTGCGTAGGGATTGACTTCCCGCGCATCATTAAAATTGGCGCTGTAAAAATATTCAACGCCATCGACTTATTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGATGCGGCGTAAACGcc                                                                                                                                                   <  1:1231694/147‑1 (MQ=255)
      ggggCTCATTCTAACAGAAAAAGAAAACGTTTGCTTAGGGAATTCCTTCGCTCTCATCATTAAATATGGCGCTGATAATATATTCAACGCCATCGACTTTTTATGACTTTGCGGCATCGGGCAATGCGTGTCCGATGCGGCGTAAACGcc                                                                                                                                                   <  1:99844/150‑1 (MQ=255)
      ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCTATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                                                                                                   <  2:133546/150‑1 (MQ=255)
      ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                                                                                                   <  1:750954/150‑1 (MQ=255)
       gggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGAGGCGGCGTAAACGCCt                                                                                                                                                  <  2:905017/150‑1 (MQ=255)
        ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt                                                                                                                                                 <  1:1351899/150‑1 (MQ=255)
           gcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGTGTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                              >  2:901585/1‑150 (MQ=255)
           gcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                              <  2:1399639/150‑1 (MQ=255)
            cATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                             >  2:750404/1‑150 (MQ=255)
                cTAACAGTAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                         <  2:74636/150‑1 (MQ=255)
                cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                         <  1:1304754/150‑1 (MQ=255)
                cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                         <  2:1572550/150‑1 (MQ=255)
                 tAACAGAAAAAGAAAACTTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcac                                                                                                                                        <  2:1197851/150‑3 (MQ=255)
                 tAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCt                                                                                                                                        >  1:1184427/1‑150 (MQ=255)
                  aaCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa                                                                                                                                       >  2:328236/1‑150 (MQ=255)
                  aaCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacg                                                                                                                                       <  1:1002886/150‑4 (MQ=255)
                  aaCAGAAAAACAAAACGTTTGAGTAGGTAATTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACATTATATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacg                                                                                                                                       <  1:265401/150‑4 (MQ=255)
                     agaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                                                    <  1:1029675/150‑1 (MQ=255)
                      gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                                   >  2:1230577/1‑150 (MQ=255)
                      gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGAGTAAACGCCTTATCCGACCTACGGt                                                                                                                                   >  2:1474037/1‑150 (MQ=255)
                      gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacgcatt                                                                                                                                   <  2:1279141/150‑8 (MQ=38)
                        aaaagcaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCCGGTTc                                                                                                                                 >  2:1590763/1‑150 (MQ=255)
                        aaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTc                                                                                                                                 <  1:1621717/150‑1 (MQ=255)
                           agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGTGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              >  2:1096529/1‑150 (MQ=255)
                           agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              >  1:699805/1‑150 (MQ=255)
                           agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              >  1:706045/1‑150 (MQ=255)
                           agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCATTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              >  1:515314/1‑150 (MQ=255)
                           agaaaaCGATTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCACCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGAAGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              <  2:342581/150‑1 (MQ=255)
                            gaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTATGGTTCTAcc                                                                                                                             <  2:1706540/150‑1 (MQ=255)
                             aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                                                                                            <  1:1601801/150‑1 (MQ=255)
                             aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                                                                                            <  1:745584/150‑1 (MQ=255)
                              aaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATCGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAcccc                                                                                                                           <  1:792130/150‑1 (MQ=255)
                                aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGTGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTg                                                                                                                         <  1:901585/150‑1 (MQ=255)
                                aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTg                                                                                                                         <  1:809769/150‑1 (MQ=255)
                                  gTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGCCTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑g                                                                                                                     >  2:350133/1‑150 (MQ=255)
                                       cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGTATGAGTCGTAAACGCCATATCCGAACTACGGTTCTACCCCTGC‑‑GTAGGc                                                                                                                >  2:36476/1‑150 (MQ=255)
                                        gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                         <  2:1568471/126‑1 (MQ=255)
                                        gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGcc                                                                                                               <  1:54982/150‑1 (MQ=255)
                                          aGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTg                                                                                                             <  2:879035/150‑1 (MQ=255)
                                          aGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTg                                                                                                             <  2:1201834/150‑1 (MQ=255)
                                           gggATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTTCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGa                                                                                                            >  2:1641401/1‑150 (MQ=255)
                                           gggATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGa                                                                                                            >  2:810461/1‑150 (MQ=255)
                                               tttCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGTCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAg                                                                                                        <  2:848088/150‑1 (MQ=255)
                                                ttccttccCGCGCATCAATAAAAATGGCGCGGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGa                                                                                                       <  1:1279718/150‑1 (MQ=255)
                                                 tccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGAc                                                                                                      <  1:990686/150‑1 (MQ=255)
                                                  ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGAcg                                                                                                     >  2:1324402/1‑150 (MQ=255)
                                                             aTCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCCGATAAGACGCGCCAGCGTCg                                                                                          <  1:1374089/150‑1 (MQ=255)
                                                              tCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGc                                                                                         <  2:326411/150‑1 (MQ=255)
                                                                aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCAt                                                                                       >  2:564171/1‑150 (MQ=255)
                                                                 aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCATTATCCGACCTACGGTTCTACCCCTGC‑‑GTCGGCCTGATAATACGCGCCAGCGTCGCATc                                                                                      >  2:121087/1‑150 (MQ=255)
                                                                    aaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg                                                                                   <  1:1004132/150‑1 (MQ=255)
                                                                     cgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                                  >  1:311983/3‑150 (MQ=37)
                                                                      gaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCAACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                                 >  1:1432264/2‑150 (MQ=37)
                                                                      gaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGAGGCATCGTGCAATGCGTATCGGATGCGTCGTAAAAGCCAGAACAGACATACGTTTATACCCCTGC‑‑GTAGGCCTGATAATAAGACCCATCGTCGCATCATGCa                                                                                 >  2:903665/2‑150 (MQ=255)
                                                                      aaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                                 <  2:1447676/150‑1 (MQ=255)
                                                                        tGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTTTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTTAACGCCTTATCCGACCTACGGTTCTACCCCGGC‑‑GTAGGCCTGCTAAGACGCGCCAGCGTCGCATCAGGCAAg                                                                               >  1:1529891/1‑150 (MQ=255)
                                                                          gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc                                                                             <  2:560763/150‑1 (MQ=255)
                                                                                aaaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                       <  2:1184427/150‑1 (MQ=255)
                                                                                aaaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                       <  1:972680/150‑1 (MQ=255)
                                                                                 aaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa                                                                      <  1:409364/150‑1 (MQ=255)
                                                                                      tatTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCg                                                                 >  1:189465/1‑150 (MQ=255)
                                                                                       atTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCgg                                                                >  2:836263/1‑150 (MQ=255)
                                                                                        ttCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGc                                                               <  2:189465/150‑1 (MQ=255)
                                                                                         tCAACGCCATCGACTTATTATGCCTTTGCTGCATCGGGCAATGCGTGTCGGATGCGGCGTAACCGCCTTATCAGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCACATCAGGCAAGACCGTATTAATTCGGCg                                                              <  1:845150/150‑1 (MQ=39)
                                                                                              gCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCTTATTAATTCGGCGtatat                                                         >  2:31740/1‑149 (MQ=255)
                                                                                              gCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTATTTCGGCGTATAg                                                         >  1:848905/1‑150 (MQ=255)
                                                                                                cATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGAAAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGcc                                                       <  1:750404/150‑1 (MQ=255)
                                                                                                  tCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCa                                                     >  2:427646/1‑150 (MQ=255)
                                                                                                       tttttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGACAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTAcc                                                <  1:329747/150‑1 (MQ=255)
                                                                                                        ttttATGCCTTTGCGGCATCGGGAAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCTACCTACGGTTCTACCCCTGC‑‑TTAGGCCTTATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCa                                               <  2:1269547/150‑1 (MQ=255)
                                                                                                         tttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCAc                                              <  2:1286373/150‑1 (MQ=255)
                                                                                                             tGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCa                                          >  2:633037/1‑150 (MQ=255)
                                                                                                                 tttGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTa                                      >  1:98355/1‑150 (MQ=255)
                                                                                                                 ttggCGGCATCGGGCAAGGCGTGTGGTAGGCGGCTAAACCGCCTTACCCGACCTACGGTTCTCCCCCGGC‑‑GTCGGCCTCAAAAGACGCCCCCGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTa                                      <  1:1572456/147‑1 (MQ=255)
                                                                                                                   tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTACACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                                    >  1:1083579/1‑150 (MQ=255)
                                                                                                                   tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                                    <  1:353923/150‑1 (MQ=255)
                                                                                                                   tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                                    <  2:904990/150‑1 (MQ=255)
                                                                                                                    gCGGCTTCGGGCATTGCGTGTGGGATGCGGCGTAAACGCCTTATCCGACCTACTGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCTTCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc                                   <  1:1474037/150‑1 (MQ=38)
                                                                                                                    gCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc                                   >  1:844241/1‑150 (MQ=255)
                                                                                                                      ggCATCGGGCAATGCGTGGCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGCTACCCCTGC‑‑GTAGGCCTGATAAGACGAGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCa                                 <  1:306169/150‑1 (MQ=255)
                                                                                                                           cGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAg                            <  1:1240513/150‑1 (MQ=255)
                                                                                                                            gggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGt                           >  1:1670726/1‑150 (MQ=255)
                                                                                                                             ggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCCCTTAAGCCAGAAAGtt                          >  1:49592/1‑150 (MQ=255)
                                                                                                                              gCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTg                         <  1:550335/150‑1 (MQ=255)
                                                                                                                              gCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAAGGCTATACACCAGC‑‑GTAGTCCTGATAAGACGCGCCAGCGACGCATCAGTCAAGAACGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTg                         <  1:121087/150‑1 (MQ=38)
                                                                                                                               cAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGc                        <  2:1122763/150‑1 (MQ=255)
                                                                                                                                    cGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGATAGTTGCTTCaa                   >  1:825763/1‑150 (MQ=255)
                                                                                                                                     gtgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGTCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAAc                  <  2:34621/150‑1 (MQ=255)
                                                                                                                                      tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt                 <  2:262989/150‑1 (MQ=255)
                                                                                                                                      ggtCGGATAAGGCGTTTACGCCGCATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt                 >  2:931663/2‑150 (MQ=39)
                                                                                                                                         cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGGATTAATTCGGAGTATAGCCCATTTCCACGCCACTTAAGCCAGCAAGTTGCTTCAACTccc              >  2:156496/1‑148 (MQ=255)
                                                                                                                                          ggataaggcgTTTACGCCGCATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCCCGCCACTTAAGCCAGAAAGTTGCGTCAACTCAcc             >  2:346670/7‑150 (MQ=38)
                                                                                                                                           gATGCGGCGCGAACGCCTTATCCGGCCTACGGTTCGGCACAAACCTGTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCAc              >  1:183212/1‑150 (MQ=18)
                                                                                                                                            ataaggcgTTTACGCCGCATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCACCTCACCCa           >  1:1576276/5‑150 (MQ=39)
                                                                                                                                              gcggcgTAAACGCCTTATCCTACCTACGGTTCTACCCCTGC‑‑GTAGGACTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGc         <  2:515314/150‑1 (MQ=255)
                                                                                                                                                 gcgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGaa      >  1:1527872/1‑150 (MQ=255)
                                                                                                                                                  cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCCCTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAAc     >  2:161982/1‑150 (MQ=255)
                                                                                                                                                  cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAAc     <  1:1096529/150‑1 (MQ=255)
                                                                                                                                                     aaaCGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCCCGCCACTTTAGCCAGAAAGTTGCGTCAACTCCCCCAGCGAACCtt  >  1:940630/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
CTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTT  >  NC_000913/2430794‑2431092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: