Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,430,944 A→T 6.7% intergenic (‑181/+78) cvpA ← / ← dedD colicin V production protein/membrane‑anchored periplasmic protein involved in septation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,9440AT 6.7% 302.8 / 8.5 104intergenic (‑181/+78)cvpA/dedDcolicin V production protein/membrane‑anchored periplasmic protein involved in septation
Reads supporting (aligned to +/- strand):  ref base A (39/57);  new base T (3/4);  total (43/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

Only 100 of 105 total aligned reads displayed.

CGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTT  >  NC_000913/2430799‑2431092
                                                                                                                                                 |                                                                                                                                                      
 ggtgcgcATTCTAACATTAAAAGAAAACGTTTGCGTAGGGATTGACTTCCCGCGCATCATTAAAATTGGCGCTGTAAAAATATTCAACGCCATCGACTTATTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGATGCGGCGTAAACGcc                                                                                                                                                   <  1:1231694/147‑1 (MQ=255)
 ggggCTCATTCTAACAGAAAAAGAAAACGTTTGCTTAGGGAATTCCTTCGCTCTCATCATTAAATATGGCGCTGATAATATATTCAACGCCATCGACTTTTTATGACTTTGCGGCATCGGGCAATGCGTGTCCGATGCGGCGTAAACGcc                                                                                                                                                   <  1:99844/150‑1 (MQ=255)
 ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCTATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                                                                                                   <  2:133546/150‑1 (MQ=255)
 ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                                                                                                   <  1:750954/150‑1 (MQ=255)
  gggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGAGGCGGCGTAAACGCCt                                                                                                                                                  <  2:905017/150‑1 (MQ=255)
   ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt                                                                                                                                                 <  1:1351899/150‑1 (MQ=255)
      gcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGTGTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                              >  2:901585/1‑150 (MQ=255)
      gcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                              <  2:1399639/150‑1 (MQ=255)
       cATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                             >  2:750404/1‑150 (MQ=255)
           cTAACAGTAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                         <  2:74636/150‑1 (MQ=255)
           cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                         <  1:1304754/150‑1 (MQ=255)
           cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                         <  2:1572550/150‑1 (MQ=255)
            tAACAGAAAAAGAAAACTTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcac                                                                                                                                        <  2:1197851/150‑3 (MQ=255)
            tAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCt                                                                                                                                        >  1:1184427/1‑150 (MQ=255)
             aaCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa                                                                                                                                       >  2:328236/1‑150 (MQ=255)
             aaCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacg                                                                                                                                       <  1:1002886/150‑4 (MQ=255)
             aaCAGAAAAACAAAACGTTTGAGTAGGTAATTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACATTATATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacg                                                                                                                                       <  1:265401/150‑4 (MQ=255)
                agaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                                                    <  1:1029675/150‑1 (MQ=255)
                 gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                                   >  2:1230577/1‑150 (MQ=255)
                 gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGAGTAAACGCCTTATCCGACCTACGGt                                                                                                                                   >  2:1474037/1‑150 (MQ=255)
                   aaaagcaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCCGGTTc                                                                                                                                 >  2:1590763/1‑150 (MQ=255)
                   aaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTc                                                                                                                                 <  1:1621717/150‑1 (MQ=255)
                   aaaagaaaaCGTTTGCGTAGGGATTTCCTTACCGCGCATCAATAAAAATGGAGCTGAAAAAATAATAAACGCAATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTTTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTc                                                                                                                                 <  2:1556281/150‑1 (MQ=39)
                      agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGTGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              >  2:1096529/1‑150 (MQ=255)
                      agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              >  1:706045/1‑150 (MQ=255)
                      agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              >  1:699805/1‑150 (MQ=255)
                      agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCATTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              >  1:515314/1‑150 (MQ=255)
                      agaaaaCGATTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCACCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGAAGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                              <  2:342581/150‑1 (MQ=255)
                       gaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTATGGTTCTAcc                                                                                                                             <  2:1706540/150‑1 (MQ=255)
                        aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                                                                                            <  1:745584/150‑1 (MQ=255)
                           aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGTGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTg                                                                                                                         <  1:901585/150‑1 (MQ=255)
                           aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTg                                                                                                                         <  1:809769/150‑1 (MQ=255)
                             gTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGCCTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑g                                                                                                                     >  2:350133/1‑150 (MQ=255)
                                  cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGTATGAGTCGTAAACGCCATATCCGAACTACGGTTCTACCCCTGC‑‑GTAGGc                                                                                                                >  2:36476/1‑150 (MQ=255)
                                   gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                         >  1:1568471/1‑126 (MQ=255)
                                   gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                         <  2:1568471/126‑1 (MQ=255)
                                   gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGcc                                                                                                               <  1:54982/150‑1 (MQ=255)
                                    tAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGTGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCt                                                                                                              <  1:984891/150‑1 (MQ=255)
                                     aGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTg                                                                                                             <  2:879035/150‑1 (MQ=255)
                                     aGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTg                                                                                                             <  2:1201834/150‑1 (MQ=255)
                                      gggATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTTCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGa                                                                                                            >  2:1641401/1‑150 (MQ=255)
                                      gggATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGa                                                                                                            >  2:810461/1‑150 (MQ=255)
                                          tttCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGTCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAg                                                                                                        <  2:848088/150‑1 (MQ=255)
                                           ttccttccCGCGCATCAATAAAAATGGCGCGGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGa                                                                                                       <  1:1279718/150‑1 (MQ=255)
                                            tccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGAc                                                                                                      <  1:990686/150‑1 (MQ=255)
                                             ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGAcg                                                                                                     >  2:1324402/1‑150 (MQ=255)
                                                tccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACgcgc                                                                                                  <  2:38540/150‑1 (MQ=255)
                                                        aTCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCCGATAAGACGCGCCAGCGTCg                                                                                          <  1:1374089/150‑1 (MQ=255)
                                                         tCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGc                                                                                         <  2:326411/150‑1 (MQ=255)
                                                           aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCAt                                                                                       >  2:564171/1‑150 (MQ=255)
                                                            aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCATTATCCGACCTACGGTTCTACCCCTGC‑‑GTCGGCCTGATAATACGCGCCAGCGTCGCATc                                                                                      >  2:121087/1‑150 (MQ=255)
                                                               aaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg                                                                                   <  1:1004132/150‑1 (MQ=255)
                                                                cgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                                  >  1:311983/3‑150 (MQ=37)
                                                                 gaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCAACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                                 >  1:1432264/2‑150 (MQ=37)
                                                                 gaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGAGGCATCGTGCAATGCGTATCGGATGCGTCGTAAAAGCCAGAACAGACATACGTTTATACCCCTGC‑‑GTAGGCCTGATAATAAGACCCATCGTCGCATCATGCa                                                                                 >  2:903665/2‑150 (MQ=255)
                                                                 aaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                                 <  2:1447676/150‑1 (MQ=255)
                                                                   tGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTTTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTTAACGCCTTATCCGACCTACGGTTCTACCCCGGC‑‑GTAGGCCTGCTAAGACGCGCCAGCGTCGCATCAGGCAAg                                                                               >  1:1529891/1‑150 (MQ=255)
                                                                     gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc                                                                             <  2:560763/150‑1 (MQ=255)
                                                                           aaaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                       <  2:1184427/150‑1 (MQ=255)
                                                                           aaaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                       <  1:972680/150‑1 (MQ=255)
                                                                            aaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa                                                                      <  1:409364/150‑1 (MQ=255)
                                                                                 tatTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCg                                                                 >  1:189465/1‑150 (MQ=255)
                                                                                  atTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCgg                                                                >  2:836263/1‑150 (MQ=255)
                                                                                   ttCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGc                                                               <  2:189465/150‑1 (MQ=255)
                                                                                    tCAACGCCATCGACTTATTATGCCTTTGCTGCATCGGGCAATGCGTGTCGGATGCGGCGTAACCGCCTTATCAGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCACATCAGGCAAGACCGTATTAATTCGGCg                                                              <  1:845150/150‑1 (MQ=39)
                                                                                         gCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCTTATTAATTCGGCGtatat                                                         >  2:31740/1‑149 (MQ=255)
                                                                                         gCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTATTTCGGCGTATAg                                                         >  1:848905/1‑150 (MQ=255)
                                                                                           cATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGAAAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGcc                                                       <  1:750404/150‑1 (MQ=255)
                                                                                             tCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCa                                                     >  2:427646/1‑150 (MQ=255)
                                                                                                  tttttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGACAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTAcc                                                <  1:329747/150‑1 (MQ=255)
                                                                                                   ttttATGCCTTTGCGGCATCGGGAAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCTACCTACGGTTCTACCCCTGC‑‑TTAGGCCTTATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCa                                               <  2:1269547/150‑1 (MQ=255)
                                                                                                    tttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCAc                                              <  2:1286373/150‑1 (MQ=255)
                                                                                                        tGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCa                                          >  2:633037/1‑150 (MQ=255)
                                                                                                            tttGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTa                                      >  1:98355/1‑150 (MQ=255)
                                                                                                            ttggCGGCATCGGGCAAGGCGTGTGGTAGGCGGCTAAACCGCCTTACCCGACCTACGGTTCTCCCCCGGC‑‑GTCGGCCTCAAAAGACGCCCCCGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTa                                      <  1:1572456/147‑1 (MQ=255)
                                                                                                              tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTACACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                                    >  1:1083579/1‑150 (MQ=255)
                                                                                                              tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                                    <  1:353923/150‑1 (MQ=255)
                                                                                                              tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                                    <  2:904990/150‑1 (MQ=255)
                                                                                                               gCGGCTTCGGGCATTGCGTGTGGGATGCGGCGTAAACGCCTTATCCGACCTACTGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCTTCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc                                   <  1:1474037/150‑1 (MQ=38)
                                                                                                               gCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc                                   >  1:844241/1‑150 (MQ=255)
                                                                                                                 ggCATCGGGCAATGCGTGGCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGCTACCCCTGC‑‑GTAGGCCTGATAAGACGAGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCa                                 <  1:306169/150‑1 (MQ=255)
                                                                                                                      cGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAg                            <  1:1240513/150‑1 (MQ=255)
                                                                                                                       gggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGt                           >  1:1670726/1‑150 (MQ=255)
                                                                                                                        ggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCCCTTAAGCCAGAAAGtt                          >  1:49592/1‑150 (MQ=255)
                                                                                                                         gCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTg                         <  1:550335/150‑1 (MQ=255)
                                                                                                                         gCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAAGGCTATACACCAGC‑‑GTAGTCCTGATAAGACGCGCCAGCGACGCATCAGTCAAGAACGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTg                         <  1:121087/150‑1 (MQ=38)
                                                                                                                          cAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGc                        <  2:1122763/150‑1 (MQ=255)
                                                                                                                           aaTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCt                       <  1:564171/150‑1 (MQ=255)
                                                                                                                               cGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGATAGTTGCTTCaa                   >  1:825763/1‑150 (MQ=255)
                                                                                                                                 tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt                 <  2:262989/150‑1 (MQ=255)
                                                                                                                                 ggtCGGATAAGGCGTTTACGCCGCATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt                 >  2:931663/2‑150 (MQ=39)
                                                                                                                                    cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGGATTAATTCGGAGTATAGCCCATTTCCACGCCACTTAAGCCAGCAAGTTGCTTCAACTccc              >  2:156496/1‑148 (MQ=255)
                                                                                                                                     ggataaggcgTTTACGCCGCATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCCCGCCACTTAAGCCAGAAAGTTGCGTCAACTCAcc             >  2:346670/7‑150 (MQ=38)
                                                                                                                                      gATGCGGCGCGAACGCCTTATCCGGCCTACGGTTCGGCACAAACCTGTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCAc              >  1:183212/1‑150 (MQ=18)
                                                                                                                                       ataaggcgTTTACGCCGCATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCACCTCACCCa           >  1:1576276/5‑150 (MQ=39)
                                                                                                                                         gcggcgTAAACGCCTTATCCTACCTACGGTTCTACCCCTGC‑‑GTAGGACTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGc         <  2:515314/150‑1 (MQ=255)
                                                                                                                                            gcgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGaa      >  1:1527872/1‑150 (MQ=255)
                                                                                                                                             cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCCCTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAAc     >  2:161982/1‑150 (MQ=255)
                                                                                                                                             cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAAc     <  1:1096529/150‑1 (MQ=255)
                                                                                                                                                aaaCGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCCCGCCACTTTAGCCAGAAAGTTGCGTCAACTCCCCCAGCGAACCtt  >  1:940630/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
CGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTT  >  NC_000913/2430799‑2431092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: