Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,430,950 T→G 6.3% intergenic (‑187/+72) cvpA ← / ← dedD colicin V production protein/membrane‑anchored periplasmic protein involved in septation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,9500TG 6.3% 281.3 / 6.6 103intergenic (‑187/+72)cvpA/dedDcolicin V production protein/membrane‑anchored periplasmic protein involved in septation
Reads supporting (aligned to +/- strand):  ref base T (40/54);  new base G (3/4);  total (45/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 106 total aligned reads displayed.

GGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCT  >  NC_000913/2430801‑2431098
                                                                                                                                                     |                                                                                                                                                      
gggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGAGGCGGCGTAAACGCCt                                                                                                                                                        <  2:905017/150‑1 (MQ=255)
 ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt                                                                                                                                                       <  1:1351899/150‑1 (MQ=255)
    gcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGTGTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                    >  2:901585/1‑150 (MQ=255)
    gcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                    <  2:1399639/150‑1 (MQ=255)
     cATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                                   >  2:750404/1‑150 (MQ=255)
         cTAACAGTAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                               <  2:74636/150‑1 (MQ=255)
         cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                               <  2:1572550/150‑1 (MQ=255)
         cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                               <  1:1304754/150‑1 (MQ=255)
          tAACAGAAAAAGAAAACTTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcac                                                                                                                                              <  2:1197851/150‑3 (MQ=255)
          tAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCt                                                                                                                                              >  1:1184427/1‑150 (MQ=255)
           aaCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa                                                                                                                                             >  2:328236/1‑150 (MQ=255)
           aaCAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacg                                                                                                                                             <  1:1002886/150‑4 (MQ=255)
           aaCAGAAAAACAAAACGTTTGAGTAGGTAATTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACATTATATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacg                                                                                                                                             <  1:265401/150‑4 (MQ=255)
              agaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                                                          <  1:1029675/150‑1 (MQ=255)
               gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                                         >  2:1230577/1‑150 (MQ=255)
               gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGAGTAAACGCCTTATCCGACCTACGGt                                                                                                                                         >  2:1474037/1‑150 (MQ=255)
               gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATAAGGCGTTTACGCCGCATCCGAcacgcatt                                                                                                                                         <  2:1279141/150‑8 (MQ=38)
                 aaaagcaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCCGGTTc                                                                                                                                       >  2:1590763/1‑150 (MQ=255)
                 aaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTc                                                                                                                                       <  1:1621717/150‑1 (MQ=255)
                    agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGTGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                                    >  2:1096529/1‑150 (MQ=255)
                    agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                                    >  1:699805/1‑150 (MQ=255)
                    agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                                    >  1:706045/1‑150 (MQ=255)
                    agaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCATTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                                    >  1:515314/1‑150 (MQ=255)
                    agaaaaCGATTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCACCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGAAGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAc                                                                                                                                    <  2:342581/150‑1 (MQ=255)
                     gaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTATGGTTCTAcc                                                                                                                                   <  2:1706540/150‑1 (MQ=255)
                      aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                                                                                                  <  1:1601801/150‑1 (MQ=255)
                      aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                                                                                                  <  1:745584/150‑1 (MQ=255)
                       aaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATCGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAcccc                                                                                                                                 <  1:792130/150‑1 (MQ=255)
                         aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGTGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTg                                                                                                                               <  1:901585/150‑1 (MQ=255)
                         aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTg                                                                                                                               <  1:809769/150‑1 (MQ=255)
                           gTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGCCTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑g                                                                                                                           >  2:350133/1‑150 (MQ=255)
                                cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGTATGAGTCGTAAACGCCATATCCGAACTACGGTTCTACCCCTGC‑‑GTAGGc                                                                                                                      >  2:36476/1‑150 (MQ=255)
                                 gTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                               <  2:1568471/126‑1 (MQ=255)
                                   aGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTg                                                                                                                   <  2:1201834/150‑1 (MQ=255)
                                   aGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTg                                                                                                                   <  2:879035/150‑1 (MQ=255)
                                    gggATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTTCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGa                                                                                                                  >  2:1641401/1‑150 (MQ=255)
                                    gggATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGa                                                                                                                  >  2:810461/1‑150 (MQ=255)
                                        tttCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGTCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAg                                                                                                              <  2:848088/150‑1 (MQ=255)
                                         ttccttccCGCGCATCAATAAAAATGGCGCGGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGa                                                                                                             <  1:1279718/150‑1 (MQ=255)
                                          tccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGAc                                                                                                            <  1:990686/150‑1 (MQ=255)
                                           ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGAcg                                                                                                           >  2:1324402/1‑150 (MQ=255)
                                                      aTCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCCGATAAGACGCGCCAGCGTCg                                                                                                <  1:1374089/150‑1 (MQ=255)
                                                       tCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGc                                                                                               <  2:326411/150‑1 (MQ=255)
                                                         aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCAt                                                                                             >  2:564171/1‑150 (MQ=255)
                                                          aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCATTATCCGACCTACGGTTCTACCCCTGC‑‑GTCGGCCTGATAATACGCGCCAGCGTCGCATc                                                                                            >  2:121087/1‑150 (MQ=255)
                                                             aaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGGGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg                                                                                         <  1:1004132/150‑1 (MQ=255)
                                                              cgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                                        >  1:311983/3‑150 (MQ=37)
                                                               gaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCAACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                                       >  1:1432264/2‑150 (MQ=37)
                                                               gaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGAGGCATCGTGCAATGCGTATCGGATGCGTCGTAAAAGCCAGAACAGACATACGTTTATACCCCTGC‑‑GTAGGCCTGATAATAAGACCCATCGTCGCATCATGCa                                                                                       >  2:903665/2‑150 (MQ=255)
                                                               aaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                                                                                       <  2:1447676/150‑1 (MQ=255)
                                                                 tGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTTTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTTAACGCCTTATCCGACCTACGGTTCTACCCCGGC‑‑GTAGGCCTGCTAAGACGCGCCAGCGTCGCATCAGGCAAg                                                                                     >  1:1529891/1‑150 (MQ=255)
                                                                   gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc                                                                                   <  2:560763/150‑1 (MQ=255)
                                                                         aaaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                             <  1:972680/150‑1 (MQ=255)
                                                                         aaaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTAtt                                                                             <  2:1184427/150‑1 (MQ=255)
                                                                          aaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa                                                                            <  1:409364/150‑1 (MQ=255)
                                                                               tatTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCg                                                                       >  1:189465/1‑150 (MQ=255)
                                                                                atTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCgg                                                                      >  2:836263/1‑150 (MQ=255)
                                                                                 ttCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGc                                                                     <  2:189465/150‑1 (MQ=255)
                                                                                  tCAACGCCATCGACTTATTATGCCTTTGCTGCATCGGGCAATGCGTGTCGGATGCGGCGTAACCGCCTTATCAGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCACATCAGGCAAGACCGTATTAATTCGGCg                                                                    <  1:845150/150‑1 (MQ=39)
                                                                                       gCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCTTATTAATTCGGCGtatat                                                               >  2:31740/1‑149 (MQ=255)
                                                                                       gCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTATTTCGGCGTATAg                                                               >  1:848905/1‑150 (MQ=255)
                                                                                         cATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGAAAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGcc                                                             <  1:750404/150‑1 (MQ=255)
                                                                                           tCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCa                                                           >  2:427646/1‑150 (MQ=255)
                                                                                                tttttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGACAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTAcc                                                      <  1:329747/150‑1 (MQ=255)
                                                                                                 ttttATGCCTTTGCGGCATCGGGAAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCTACCTACGGTTCTACCCCTGC‑‑TTAGGCCTTATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCa                                                     <  2:1269547/150‑1 (MQ=255)
                                                                                                  tttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCAc                                                    <  2:1286373/150‑1 (MQ=255)
                                                                                                      tGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCa                                                >  2:633037/1‑150 (MQ=255)
                                                                                                          tttGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTa                                            >  1:98355/1‑150 (MQ=255)
                                                                                                          ttggCGGCATCGGGCAAGGCGTGTGGTAGGCGGCTAAACCGCCTTACCCGACCTACGGTTCTCCCCCGGC‑‑GTCGGCCTCAAAAGACGCCCCCGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTa                                            <  1:1572456/147‑1 (MQ=255)
                                                                                                            tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTACACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                                          >  1:1083579/1‑150 (MQ=255)
                                                                                                            tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                                          <  1:353923/150‑1 (MQ=255)
                                                                                                            tGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAg                                          <  2:904990/150‑1 (MQ=255)
                                                                                                             gCGGCTTCGGGCATTGCGTGTGGGATGCGGCGTAAACGCCTTATCCGACCTACTGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCTTCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc                                         <  1:1474037/150‑1 (MQ=38)
                                                                                                             gCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGc                                         >  1:844241/1‑150 (MQ=255)
                                                                                                               ggCATCGGGCAATGCGTGGCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGCTACCCCTGC‑‑GTAGGCCTGATAAGACGAGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCa                                       <  1:306169/150‑1 (MQ=255)
                                                                                                                    cGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAg                                  <  1:1240513/150‑1 (MQ=255)
                                                                                                                     gggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGt                                 >  1:1670726/1‑150 (MQ=255)
                                                                                                                      ggCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCCCTTAAGCCAGAAAGtt                                >  1:49592/1‑150 (MQ=255)
                                                                                                                       gCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTg                               <  1:550335/150‑1 (MQ=255)
                                                                                                                       gCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAAGGCTATACACCAGC‑‑GTAGTCCTGATAAGACGCGCCAGCGACGCATCAGTCAAGAACGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTg                               <  1:121087/150‑1 (MQ=38)
                                                                                                                        cAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGc                              <  2:1122763/150‑1 (MQ=255)
                                                                                                                         aaTGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCt                             <  1:564171/150‑1 (MQ=255)
                                                                                                                             cGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGATAGTTGCTTCaa                         >  1:825763/1‑150 (MQ=255)
                                                                                                                              gtgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGTCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAAc                        <  2:34621/150‑1 (MQ=255)
                                                                                                                               tgtCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACt                       <  2:262989/150‑1 (MQ=255)
                                                                                                                                  cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGGATTAATTCGGAGTATAGCCCATTTCCACGCCACTTAAGCCAGCAAGTTGCTTCAACTccc                    >  2:156496/1‑148 (MQ=255)
                                                                                                                                   ggataaggcgTTTACGCCGCATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCCCGCCACTTAAGCCAGAAAGTTGCGTCAACTCAcc                   >  2:346670/7‑150 (MQ=38)
                                                                                                                                    gATGCGGCGCGAACGCCTTATCCGGCCTACGGTTCGGCACAAACCTGTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCAc                    >  1:183212/1‑150 (MQ=18)
                                                                                                                                     ataaggcgTTTACGCCGCATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCACCTCACCCa                 >  1:1576276/5‑150 (MQ=39)
                                                                                                                                       gcggcgTAAACGCCTTATCCTACCTACGGTTCTACCCCTGC‑‑GTAGGACTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGc               <  2:515314/150‑1 (MQ=255)
                                                                                                                                          gcgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGaa            >  1:1527872/1‑150 (MQ=255)
                                                                                                                                           cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCCCTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAAc           >  2:161982/1‑150 (MQ=255)
                                                                                                                                           cgTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAAc           <  1:1096529/150‑1 (MQ=255)
                                                                                                                                              aaaCGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCCCGCCACTTTAGCCAGAAAGTTGCGTCAACTCCCCCAGCGAACCtt        >  1:940630/1‑150 (MQ=255)
                                                                                                                                                  gCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCTCTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAg    <  1:1236221/150‑1 (MQ=255)
                                                                                                                                                   ccTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCCACTCACCCAGCGCACCTTTCAGc   >  2:1170595/1‑150 (MQ=255)
                                                                                                                                                   ccTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGc   <  2:661657/150‑1 (MQ=255)
                                                                                                                                                   ccTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGc   <  1:231499/150‑1 (MQ=255)
                                                                                                                                                   ccTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGc   >  2:1116210/1‑150 (MQ=255)
                                                                                                                                                    tttATCCTACATACGGTACAACCCCTGG‑‑GTAGGCATTATAATACGCGCCAGCTTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCt  <  1:1570810/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGC‑‑GTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCT  >  NC_000913/2430801‑2431098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: