Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,438,871 A→T 7.5% N308Y (AAT→TAT)  flk → putative flagella assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,438,8710AT 7.5% 298.4 / 11.5 107N308Y (AAT→TAT) flkputative flagella assembly protein
Reads supporting (aligned to +/- strand):  ref base A (43/56);  new base T (3/5);  total (46/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

Only 100 of 111 total aligned reads displayed.

TATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAG  >  NC_000913/2438724‑2439020
                                                                                                                                                   |                                                                                                                                                     
tatGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGTCACGTTGCAAAat                                                                                                                                                     >  2:1088828/1‑150 (MQ=255)
 atGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGAGTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAata                                                                                                                                                    <  2:1071601/150‑1 (MQ=255)
  tGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAatat                                                                                                                                                   <  1:213375/150‑1 (MQ=255)
   ggTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATg                                                                                                                                                  >  2:1617003/1‑150 (MQ=255)
    gTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATgg                                                                                                                                                 >  1:497195/1‑150 (MQ=255)
       tttCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCaa                                                                                                                                              >  2:815719/1‑150 (MQ=255)
          cTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAAcgc                                                                                                                                           >  2:1447256/1‑150 (MQ=255)
           tGCGCAATCATCTCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACAGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAAcgcg                                                                                                                                          <  2:85947/150‑1 (MQ=255)
           tGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAAcgcg                                                                                                                                          >  1:1476629/1‑150 (MQ=255)
            gcgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGt                                                                                                                                         >  2:141492/1‑150 (MQ=255)
             cgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTc                                                                                                                                        <  1:1458555/150‑1 (MQ=255)
              gcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTcc                                                                                                                                       <  2:1424108/150‑1 (MQ=255)
              gcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTcc                                                                                                                                       <  1:1252482/150‑1 (MQ=255)
               cAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTccc                                                                                                                                      <  1:672922/150‑1 (MQ=255)
                aaTCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCcg                                                                                                                                     <  2:923285/150‑1 (MQ=255)
                   catcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCgcgc                                                                                                                                  <  1:1651509/150‑1 (MQ=255)
                   catcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGAGGGACGCGTTGCCATATTTTGCAACGTGTccatcat                                                                                                                                  <  1:978873/150‑6 (MQ=38)
                    atcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCt                                                                                                                                 <  1:620834/150‑1 (MQ=255)
                     tcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCtg                                                                                                                                >  2:768164/1‑150 (MQ=255)
                      cGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCtgt                                                                                                                               <  2:1196471/150‑1 (MQ=255)
                        cTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTgg                                                                                                                             <  1:264788/150‑1 (MQ=255)
                              tctGCCCAGTCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGTATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTg                                                                                                                       <  2:254571/150‑1 (MQ=255)
                                tGCCCATGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                     <  1:43611/150‑1 (MQ=255)
                                tGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                     <  1:1346128/150‑1 (MQ=255)
                                 gCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTa                                                                                                                    >  2:479941/1‑150 (MQ=255)
                                  cccAGGCCGAGGATATTCTTTATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGTTACTGTTAg                                                                                                                   <  1:1057179/150‑1 (MQ=255)
                                     aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATTCAACCTCTGCTTAATCGTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcg                                                                                                                <  2:434495/150‑1 (MQ=255)
                                     aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcg                                                                                                                <  1:979930/150‑1 (MQ=255)
                                      gtccGAGGATATTATAAATCAGTTTTATCAACGGCGGGTTCACGTTATTGTTCCTCTTGTTATGCTACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATAGGGCAACGCGTCCCGCGCTGTGGATAATGTTAGTcgc                                                                                                               <  1:89913/148‑1 (MQ=255)
                                       gCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcgcg                                                                                                              <  1:1385160/150‑1 (MQ=255)
                                         cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                            <  1:656010/150‑1 (MQ=255)
                                         cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                            <  1:1220305/150‑1 (MQ=255)
                                         cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                            <  2:599310/150‑1 (MQ=255)
                                          gAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAtt                                                                                                           >  2:1411006/1‑150 (MQ=255)
                                            ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCATAAAATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAGTag                                                                                                         >  2:320255/1‑149 (MQ=255)
                                            ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTACGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGAt                                                                                                                           <  1:1623719/132‑1 (MQ=255)
                                            ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTAAGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGAt                                                                                                                           >  2:1623719/1‑132 (MQ=255)
                                              atcttCTTAATCAGATTAATCCCCGGCGGGTAAAAGGTCTTGATCCGCGGTTTATGCAACCGATGCTTAATCCTTTACCACCGATGATGGACACGTAGCAAAATATCGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATcc                                                                                                       <  1:306410/147‑1 (MQ=255)
                                                   cTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATg                                                                                                  <  1:1045434/150‑1 (MQ=255)
                                                      aaTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCACCGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTg                                                                                               <  1:1076368/150‑1 (MQ=255)
                                                      aaTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTg                                                                                               >  1:821551/1‑150 (MQ=255)
                                                       aTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTgg                                                                                              >  2:891252/1‑150 (MQ=255)
                                                           gCTTTATCAACGGCGTGTAAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTAAATCCTTTTCCACCGATGATGGACACGTTGAAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCATGATGCTTGTctg                                                                                          >  1:1339285/1‑150 (MQ=255)
                                                               tATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctggctg                                                                                      <  1:1617003/150‑1 (MQ=255)
                                                                atcatcGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctggctgg                                                                                     <  2:338704/150‑1 (MQ=255)
                                                                 tCAACGGCGGGATAAAGGGATTGATCCGCGTGTTAAGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATAAGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTAACCTGATGCTGGTCTGGCTGGt                                                                                    >  1:625601/1‑150 (MQ=255)
                                                                  cAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGtt                                                                                   >  2:1724840/1‑150 (MQ=255)
                                                                     cggcggGTTAAAGGGATTGATCCGCGTGGTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTGGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGt                                                                                >  2:930100/1‑150 (MQ=255)
                                                                       gcggGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTa                                                                              >  2:610868/1‑150 (MQ=255)
                                                                          ggTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcc                                                                           <  1:1411006/150‑1 (MQ=255)
                                                                           gTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAccc                                                                          <  1:141492/150‑1 (MQ=255)
                                                                            ttAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcccc                                                                         <  2:849975/150‑1 (MQ=255)
                                                                              aaaGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcga                                                                       >  2:1405177/1‑150 (MQ=255)
                                                                                aGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcgacg                                                                     >  2:1557548/1‑150 (MQ=255)
                                                                                 gggATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                     <  1:1123355/101‑1 (MQ=255)
                                                                                 gggATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                     >  2:1123355/1‑101 (MQ=255)
                                                                                   gATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGaaa                                                                  <  2:860881/150‑1 (MQ=255)
                                                                                    aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                 <  2:1306384/150‑1 (MQ=255)
                                                                                    aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                 <  2:786084/150‑1 (MQ=255)
                                                                                    aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                 <  1:515100/150‑1 (MQ=255)
                                                                                       gATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGAc                                                              >  1:560913/1‑150 (MQ=255)
                                                                                       gATCCGCGTGTTATGCAACCGCTACTTAATCCTTTTCCACCGATGATGGACACGTGGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGAc                                                              <  1:1215769/150‑1 (MQ=255)
                                                                                         tCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                                            <  1:1147216/150‑1 (MQ=255)
                                                                                         tCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                                            >  2:901371/1‑150 (MQ=255)
                                                                                         tCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                                            <  2:1660832/150‑1 (MQ=255)
                                                                                           cgcgTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTa                                                          >  2:1430081/1‑150 (MQ=255)
                                                                                             cggggtATGCAACCGCTGCTTAATCCTTTTCCACCGATGATTGACACGTTGCAAAATATGGCAAAGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATc                                                        <  2:66287/145‑1 (MQ=255)
                                                                                              gtgtTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGACCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCg                                                       >  1:715114/1‑150 (MQ=255)
                                                                                               tgtTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGAAACGCGTCCAGCGCTGTGGTTACTGTTAGTCGCGTTTATCCTGATGCTGGTGTGGCTGGTTCGTTTTCACGGAGGAATTGACAGTATGGt                                                      >  1:418380/1‑150 (MQ=255)
                                                                                                gtTATGAAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTGGCAAAATTTGGCATCGGGTCCCGCGCTGTGGATACTGTTTGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTGGTTAACCCCGACGAAATGACAGTATCGTg                                                     <  2:950704/150‑1 (MQ=255)
                                                                                                  tATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGAc                                                   <  1:1393201/150‑1 (MQ=255)
                                                                                                   aTGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCt                                                                                                >  1:1649377/1‑104 (MQ=255)
                                                                                                   aTGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCt                                                                                                <  2:1649377/104‑1 (MQ=255)
                                                                                                   aTGCAACCGCTGCTTAATCCTTTTCCACCGATGACGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACa                                                  <  1:1398503/150‑1 (MQ=255)
                                                                                                    tGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaa                                                 <  2:1476629/150‑1 (MQ=255)
                                                                                                     gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                >  2:1673570/1‑150 (MQ=255)
                                                                                                     gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                >  1:1330841/1‑150 (MQ=255)
                                                                                                     gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCGGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                <  2:1534935/150‑1 (MQ=255)
                                                                                                        aCCGCTGCTAAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGAGCTGTTGATACTGTTAGTCGCGATTATCCTGATGCTCGTCTGGCTCGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaataat                                             >  2:1560633/1‑150 (MQ=255)
                                                                                                          cGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAc                                           <  1:441582/150‑1 (MQ=255)
                                                                                                            ctgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAccc                                         <  2:560913/150‑1 (MQ=255)
                                                                                                             tgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCa                                        <  2:497195/150‑1 (MQ=255)
                                                                                                              gcgtAATCCTTTTCCACCGATGATCGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGGCTGGCTGGCTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAg                                       <  2:1447547/147‑1 (MQ=255)
                                                                                                                  aaTCCTTTTCCACCGATGATTGACACGTTGCAAAATATGGCAACGGGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCAcc                                   <  2:291358/150‑1 (MQ=255)
                                                                                                                    tCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGc                                 >  1:779665/1‑150 (MQ=255)
                                                                                                                      cTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCag                               <  1:769139/150‑1 (MQ=255)
                                                                                                                            cACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGTAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCg                         >  2:227271/1‑150 (MQ=255)
                                                                                                                              ccGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGcc                       <  1:1557548/150‑1 (MQ=255)
                                                                                                                               cGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGccc                      <  2:1570696/150‑1 (MQ=255)
                                                                                                                                     tgGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGCCAATAAGACCCAGCACCGCCGAGATCGCCCCGGCaa                >  2:930834/1‑150 (MQ=255)
                                                                                                                                      ggACGCGTTGCCATATTTTGCAACGTGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTACCCCCGACGAAATGACAGTATCGTGACAATAATACCCCGCACCGCAGACATCGCCCCGGCAAg               >  2:1242592/1‑150 (MQ=255)
                                                                                                                                       gACGCGTTGCCATATTTTGCAACGTGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGa              >  1:306598/1‑150 (MQ=255)
                                                                                                                                         cgcGTTGCCATATTTTGCAACGTGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGaaa            >  1:678909/3‑150 (MQ=255)
                                                                                                                                         cacGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGaaa            >  2:1585642/1‑150 (MQ=255)
                                                                                                                                           cGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATa          >  1:746615/1‑150 (MQ=255)
                                                                                                                                                cAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGaa     >  2:1101672/1‑150 (MQ=255)
                                                                                                                                                 aaaaTATGGCAACGCGTCACGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTGTGGCTGGTGCGGACACCCCGACGAAATGACAGTATCGTGACAAAAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATTCCGAag    <  1:1102885/150‑1 (MQ=255)
                                                                                                                                                  aaaTATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAaga   >  1:518680/1‑150 (MQ=255)
                                                                                                                                                   aaTCTGTCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCTATTATCCTGATGTTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAagag  <  2:1702829/150‑1 (MQ=255)
                                                                                                                                                   aaTATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAagag  >  2:218827/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAG  >  NC_000913/2438724‑2439020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: