Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,438,874 A→T 8.0% M309L (ATG→TTG) ‡ flk → putative flagella assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,438,8740AT 8.0% 299.4 / 12.0 108M309L (ATG→TTG) ‡flkputative flagella assembly protein
Reads supporting (aligned to +/- strand):  ref base A (44/54);  new base T (3/6);  total (47/61)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

Only 100 of 110 total aligned reads displayed.

ATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAG  >  NC_000913/2438725‑2439020
                                                                                                                                                     |                                                                                                                                                  
atGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGAGTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAata                                                                                                                                                    <  2:1071601/150‑1 (MQ=255)
 tGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAatat                                                                                                                                                   <  1:213375/150‑1 (MQ=255)
  ggTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATg                                                                                                                                                  >  2:1617003/1‑150 (MQ=255)
   gTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATgg                                                                                                                                                 >  1:497195/1‑150 (MQ=255)
          tGCGCAATCATCTCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACAGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAAcgcg                                                                                                                                          <  2:85947/150‑1 (MQ=255)
          tGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAAcgcg                                                                                                                                          >  1:1476629/1‑150 (MQ=255)
           gcgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGt                                                                                                                                         >  2:141492/1‑150 (MQ=255)
            cgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTc                                                                                                                                        <  1:1458555/150‑1 (MQ=255)
             gcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTcc                                                                                                                                       <  2:1351555/150‑1 (MQ=255)
             gcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTcc                                                                                                                                       <  1:1252482/150‑1 (MQ=255)
             gcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTcc                                                                                                                                       <  2:1424108/150‑1 (MQ=255)
              cAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTccc                                                                                                                                      >  2:441582/1‑150 (MQ=255)
              cAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTccc                                                                                                                                      <  1:672922/150‑1 (MQ=255)
               aaTCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCcg                                                                                                                                     <  2:923285/150‑1 (MQ=255)
                  catcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCgcgc                                                                                                                                  <  1:1651509/150‑1 (MQ=255)
                  catcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGAGGGACGCGTTGCCATATTTTGCAACGTGTccatcat                                                                                                                                  <  1:978873/150‑6 (MQ=38)
                   atcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCt                                                                                                                                 <  1:620834/150‑1 (MQ=255)
                    tcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCtg                                                                                                                                >  2:768164/1‑150 (MQ=255)
                     cGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCtgt                                                                                                                               <  2:1196471/150‑1 (MQ=255)
                       cTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTgg                                                                                                                             <  1:264788/150‑1 (MQ=255)
                             tctGCCCAGTCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGTATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTg                                                                                                                       <  2:254571/150‑1 (MQ=255)
                               tGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                     <  1:1346128/150‑1 (MQ=255)
                                gCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTa                                                                                                                    >  2:479941/1‑150 (MQ=255)
                                 cccAGGCCGAGGATATTCTTTATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGTTACTGTTAg                                                                                                                   <  1:1057179/150‑1 (MQ=255)
                                    aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATTCAACCTCTGCTTAATCGTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcg                                                                                                                <  2:434495/150‑1 (MQ=255)
                                    aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcg                                                                                                                <  1:979930/150‑1 (MQ=255)
                                     gtccGAGGATATTATAAATCAGTTTTATCAACGGCGGGTTCACGTTATTGTTCCTCTTGTTATGCTACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATAGGGCAACGCGTCCCGCGCTGTGGATAATGTTAGTcgc                                                                                                               <  1:89913/148‑1 (MQ=255)
                                      gCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcgcg                                                                                                              <  1:918458/150‑1 (MQ=255)
                                      gCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcgcg                                                                                                              <  1:1385160/150‑1 (MQ=255)
                                        cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                            <  2:599310/150‑1 (MQ=255)
                                        cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                            <  1:1220305/150‑1 (MQ=255)
                                        cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                            <  1:656010/150‑1 (MQ=255)
                                         gAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAtt                                                                                                           >  2:1411006/1‑150 (MQ=255)
                                           ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCATAAAATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAGTag                                                                                                         >  2:320255/1‑149 (MQ=255)
                                           ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTACGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGAt                                                                                                                           <  1:1623719/132‑1 (MQ=255)
                                           ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTAAGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGAt                                                                                                                           >  2:1623719/1‑132 (MQ=255)
                                            gATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATc                                                                                                        >  2:1542327/1‑150 (MQ=255)
                                             atcttCTTAATCAGATTAATCCCCGGCGGGTAAAAGGTCTTGATCCGCGGTTTATGCAACCGATGCTTAATCCTTTACCACCGATGATGGACACGTAGCAAAATATCGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATcc                                                                                                       <  1:306410/147‑1 (MQ=255)
                                                  cTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATg                                                                                                  <  1:1045434/150‑1 (MQ=255)
                                                     aaTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCACCGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTg                                                                                               <  1:1076368/150‑1 (MQ=255)
                                                     aaTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTg                                                                                               >  1:821551/1‑150 (MQ=255)
                                                      aTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTgg                                                                                              >  2:891252/1‑150 (MQ=255)
                                                          gCTTTATCAACGGCGTGTAAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTAAATCCTTTTCCACCGATGATGGACACGTTGAAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCATGATGCTTGTctg                                                                                          >  1:1339285/1‑150 (MQ=255)
                                                              tATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctggctg                                                                                      <  1:1617003/150‑1 (MQ=255)
                                                                tCAACGGCGGGATAAAGGGATTGATCCGCGTGTTAAGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATAAGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTAACCTGATGCTGGTCTGGCTGGt                                                                                    >  1:625601/1‑150 (MQ=255)
                                                                 cAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGtt                                                                                   >  2:1724840/1‑150 (MQ=255)
                                                                    cggcggGTTAAAGGGATTGATCCGCGTGGTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTGGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGt                                                                                >  2:930100/1‑150 (MQ=255)
                                                                      gcggGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTa                                                                              >  2:610868/1‑150 (MQ=255)
                                                                         ggTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcc                                                                           <  1:1411006/150‑1 (MQ=255)
                                                                          gTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAccc                                                                          <  1:141492/150‑1 (MQ=255)
                                                                           ttAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcccc                                                                         <  2:849975/150‑1 (MQ=255)
                                                                             aaaGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcga                                                                       >  2:1405177/1‑150 (MQ=255)
                                                                              aaGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTTGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcgac                                                                      >  2:838544/1‑150 (MQ=255)
                                                                               aGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcgacg                                                                     >  2:1557548/1‑150 (MQ=255)
                                                                                gggATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                     >  2:1123355/1‑101 (MQ=255)
                                                                                gggATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                     <  1:1123355/101‑1 (MQ=255)
                                                                                  gATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGaaa                                                                  <  2:860881/150‑1 (MQ=255)
                                                                                   aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                 <  2:1306384/150‑1 (MQ=255)
                                                                                   aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                 <  1:515100/150‑1 (MQ=255)
                                                                                   aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                 <  2:786084/150‑1 (MQ=255)
                                                                                      gATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGAc                                                              >  1:560913/1‑150 (MQ=255)
                                                                                      gATCCGCGTGTTATGCAACCGCTACTTAATCCTTTTCCACCGATGATGGACACGTGGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGAc                                                              <  1:1215769/150‑1 (MQ=255)
                                                                                        tCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                                            <  1:1147216/150‑1 (MQ=255)
                                                                                        tCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                                            >  2:901371/1‑150 (MQ=255)
                                                                                        tCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                                            <  2:1660832/150‑1 (MQ=255)
                                                                                          cgcgTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTa                                                          >  2:1430081/1‑150 (MQ=255)
                                                                                            cggggtATGCAACCGCTGCTTAATCCTTTTCCACCGATGATTGACACGTTGCAAAATATGGCAAAGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATc                                                        <  2:66287/145‑1 (MQ=255)
                                                                                            cgTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGGCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATc                                                        <  1:374283/150‑1 (MQ=255)
                                                                                             gtgtTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGACCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCg                                                       >  1:715114/1‑150 (MQ=255)
                                                                                              tgtTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGAAACGCGTCCAGCGCTGTGGTTACTGTTAGTCGCGTTTATCCTGATGCTGGTGTGGCTGGTTCGTTTTCACGGAGGAATTGACAGTATGGt                                                      >  1:418380/1‑150 (MQ=255)
                                                                                               gtTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTg                                                     <  2:170096/150‑1 (MQ=255)
                                                                                               gtTATGAAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTGGCAAAATTTGGCATCGGGTCCCGCGCTGTGGATACTGTTTGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTGGTTAACCCCGACGAAATGACAGTATCGTg                                                     <  2:950704/150‑1 (MQ=255)
                                                                                                 tATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGAc                                                   <  1:1393201/150‑1 (MQ=255)
                                                                                                  aTGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCt                                                                                                >  1:1649377/1‑104 (MQ=255)
                                                                                                  aTGCAACCGCTGCTTAATCCTTTTCCACCGATGACGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACa                                                  <  1:1398503/150‑1 (MQ=255)
                                                                                                   tGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaa                                                 <  2:1476629/150‑1 (MQ=255)
                                                                                                    gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                >  2:1673570/1‑150 (MQ=255)
                                                                                                    gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                >  1:1330841/1‑150 (MQ=255)
                                                                                                    gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCGGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                <  2:1534935/150‑1 (MQ=255)
                                                                                                       aCCGCTGCTAAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGAGCTGTTGATACTGTTAGTCGCGATTATCCTGATGCTCGTCTGGCTCGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaataat                                             >  2:1560633/1‑150 (MQ=255)
                                                                                                       aCCGCTCCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaataat                                             >  1:148132/1‑150 (MQ=255)
                                                                                                         cGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAc                                           <  1:441582/150‑1 (MQ=255)
                                                                                                           ctgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAccc                                         <  2:560913/150‑1 (MQ=255)
                                                                                                            tgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCa                                        <  2:497195/150‑1 (MQ=255)
                                                                                                             gcgtAATCCTTTTCCACCGATGATCGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGGCTGGCTGGCTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAg                                       <  2:1447547/147‑1 (MQ=255)
                                                                                                                 aaTCCTTTTCCACCGATGATTGACACGTTGCAAAATATGGCAACGGGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCAcc                                   <  2:291358/150‑1 (MQ=255)
                                                                                                                   tCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGc                                 >  1:779665/1‑150 (MQ=255)
                                                                                                                     cTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCag                               <  1:769139/150‑1 (MQ=255)
                                                                                                                      ttttCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCaga                              >  1:64924/1‑150 (MQ=255)
                                                                                                                           cACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGTAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCg                         >  2:227271/1‑150 (MQ=255)
                                                                                                                              cGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGccc                      <  2:1570696/150‑1 (MQ=255)
                                                                                                                                    tgGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGCCAATAAGACCCAGCACCGCCGAGATCGCCCCGGCaa                >  2:930834/1‑150 (MQ=255)
                                                                                                                                     ggACGCGTTGCCATATTTTGCAACGTGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTACCCCCGACGAAATGACAGTATCGTGACAATAATACCCCGCACCGCAGACATCGCCCCGGCAAg               >  2:1242592/1‑150 (MQ=255)
                                                                                                                                      gACGCGTTGCCATATTTTGCAACGTGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGa              >  1:306598/1‑150 (MQ=255)
                                                                                                                                        cacGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGaaa            >  2:1585642/1‑150 (MQ=255)
                                                                                                                                          cGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATa          >  1:746615/1‑150 (MQ=255)
                                                                                                                                                aaaaTATGGCAACGCGTCACGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTGTGGCTGGTGCGGACACCCCGACGAAATGACAGTATCGTGACAAAAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATTCCGAag    <  1:1102885/150‑1 (MQ=255)
                                                                                                                                                 aaaTATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAaga   >  1:518680/1‑150 (MQ=255)
                                                                                                                                                  aaTCTGTCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCTATTATCCTGATGTTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAagag  <  2:1702829/150‑1 (MQ=255)
                                                                                                                                                  aaTATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAagag  >  2:218827/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAG  >  NC_000913/2438725‑2439020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: