Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,438,883 C→T 8.1% R312C (CGT→TGT)  flk → putative flagella assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,438,8830CT 8.1% 299.9 / 11.0 102R312C (CGT→TGT) flkputative flagella assembly protein
Reads supporting (aligned to +/- strand):  ref base C (39/53);  new base T (3/5);  total (42/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 107 total aligned reads displayed.

CTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAGTAACCAAACGTG  >  NC_000913/2438734‑2439032
                                                                                                                                                     |                                                                                                                                                     
cTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAAcgc                                                                                                                                                       >  2:1447256/1‑150 (MQ=255)
 tGCGCAATCATCTCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACAGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAAcgcg                                                                                                                                                      <  2:85947/150‑1 (MQ=255)
 tGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAAcgcg                                                                                                                                                      >  1:1476629/1‑150 (MQ=255)
  gcgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGt                                                                                                                                                     >  2:141492/1‑150 (MQ=255)
   cgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTc                                                                                                                                                    <  1:1458555/150‑1 (MQ=255)
    gcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTcc                                                                                                                                                   <  2:1351555/150‑1 (MQ=255)
    gcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACGCGTTGCCATATTTTGCAACGTGTcc                                                                                                                                                   <  1:1252482/150‑1 (MQ=255)
     cAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTccc                                                                                                                                                  <  1:672922/150‑1 (MQ=255)
     cAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTccc                                                                                                                                                  >  2:441582/1‑150 (MQ=255)
      aaTCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCcg                                                                                                                                                 <  2:923285/150‑1 (MQ=255)
         catcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCgcgc                                                                                                                                              <  1:1651509/150‑1 (MQ=255)
         catcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGAGGGACGCGTTGCCATATTTTGCAACGTGTccatcat                                                                                                                                              <  1:978873/150‑6 (MQ=38)
          atcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCt                                                                                                                                             <  1:620834/150‑1 (MQ=255)
           tcGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCtg                                                                                                                                            >  2:768164/1‑150 (MQ=255)
            cGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCtgt                                                                                                                                           <  2:1196471/150‑1 (MQ=255)
              cTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTgg                                                                                                                                         <  1:264788/150‑1 (MQ=255)
                    tctGCCCAGTCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGTATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTg                                                                                                                                   <  2:254571/150‑1 (MQ=255)
                      tGCCCATGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                                 <  1:43611/150‑1 (MQ=255)
                      tGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                                 <  1:1346128/150‑1 (MQ=255)
                       gCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTa                                                                                                                                >  2:479941/1‑150 (MQ=255)
                        cccAGGCCGAGGATATTCTTTATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGTTACTGTTAg                                                                                                                               <  1:1057179/150‑1 (MQ=255)
                           aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATTCAACCTCTGCTTAATCGTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcg                                                                                                                            <  2:434495/150‑1 (MQ=255)
                           aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcg                                                                                                                            <  1:979930/150‑1 (MQ=255)
                            gtccGAGGATATTATAAATCAGTTTTATCAACGGCGGGTTCACGTTATTGTTCCTCTTGTTATGCTACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATAGGGCAACGCGTCCCGCGCTGTGGATAATGTTAGTcgc                                                                                                                           <  1:89913/148‑1 (MQ=255)
                             gCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcgcg                                                                                                                          <  1:918458/150‑1 (MQ=255)
                             gCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcgcg                                                                                                                          <  1:1385160/150‑1 (MQ=255)
                               cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                                        <  1:1220305/150‑1 (MQ=255)
                               cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                                        <  1:656010/150‑1 (MQ=255)
                               cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAt                                                                                                                        <  2:599310/150‑1 (MQ=255)
                                gAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAtt                                                                                                                       >  2:1411006/1‑150 (MQ=255)
                                  ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCATAAAATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAGTag                                                                                                                     >  2:320255/1‑149 (MQ=255)
                                  ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTACGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGAt                                                                                                                                       <  1:1623719/132‑1 (MQ=255)
                                  ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTAAGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGAt                                                                                                                                       >  2:1623719/1‑132 (MQ=255)
                                   gATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATc                                                                                                                    >  2:1542327/1‑150 (MQ=255)
                                    atcttCTTAATCAGATTAATCCCCGGCGGGTAAAAGGTCTTGATCCGCGGTTTATGCAACCGATGCTTAATCCTTTACCACCGATGATGGACACGTAGCAAAATATCGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATcc                                                                                                                   <  1:306410/147‑1 (MQ=255)
                                         cTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATg                                                                                                              <  1:1045434/150‑1 (MQ=255)
                                            aaTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCACCGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTg                                                                                                           <  1:1076368/150‑1 (MQ=255)
                                            aaTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTg                                                                                                           >  1:821551/1‑150 (MQ=255)
                                             aTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTgg                                                                                                          >  2:891252/1‑150 (MQ=255)
                                                 gCTTTATCAACGGCGTGTAAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTAAATCCTTTTCCACCGATGATGGACACGTTGAAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCATGATGCTTGTctg                                                                                                      >  1:1339285/1‑150 (MQ=255)
                                                     tATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctggctg                                                                                                  <  1:1617003/150‑1 (MQ=255)
                                                      atcatcGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctggctgg                                                                                                 <  2:338704/150‑1 (MQ=255)
                                                       tCAACGGCGGGATAAAGGGATTGATCCGCGTGTTAAGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATAAGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTAACCTGATGCTGGTCTGGCTGGt                                                                                                >  1:625601/1‑150 (MQ=255)
                                                        cAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGtt                                                                                               >  2:1724840/1‑150 (MQ=255)
                                                           cggcggGTTAAAGGGATTGATCCGCGTGGTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTGGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGt                                                                                            >  2:930100/1‑150 (MQ=255)
                                                                ggTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcc                                                                                       <  1:1411006/150‑1 (MQ=255)
                                                                 gTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAccc                                                                                      <  1:141492/150‑1 (MQ=255)
                                                                  ttAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcccc                                                                                     <  2:849975/150‑1 (MQ=255)
                                                                    aaaGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcga                                                                                   >  2:1405177/1‑150 (MQ=255)
                                                                     aaGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTTGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcgac                                                                                  >  2:838544/1‑150 (MQ=255)
                                                                      aGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcgacg                                                                                 >  2:1557548/1‑150 (MQ=255)
                                                                       gggATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                                 >  2:1123355/1‑101 (MQ=255)
                                                                       gggATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGtt                                                                                                                                 <  1:1123355/101‑1 (MQ=255)
                                                                         gATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGaaa                                                                              <  2:860881/150‑1 (MQ=255)
                                                                          aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                             <  2:1306384/150‑1 (MQ=255)
                                                                          aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                             <  2:786084/150‑1 (MQ=255)
                                                                          aTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAAt                                                                             <  1:515100/150‑1 (MQ=255)
                                                                             gATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGAc                                                                          >  1:560913/1‑150 (MQ=255)
                                                                             gATCCGCGTGTTATGCAACCGCTACTTAATCCTTTTCCACCGATGATGGACACGTGGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGAc                                                                          <  1:1215769/150‑1 (MQ=255)
                                                                               tCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                                                        >  2:901371/1‑150 (MQ=255)
                                                                               tCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                                                        <  1:1147216/150‑1 (MQ=255)
                                                                               tCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                                                        <  2:1660832/150‑1 (MQ=255)
                                                                                 cgcgTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTa                                                                      >  2:1430081/1‑150 (MQ=255)
                                                                                   cggggtATGCAACCGCTGCTTAATCCTTTTCCACCGATGATTGACACGTTGCAAAATATGGCAAAGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATc                                                                    <  2:66287/145‑1 (MQ=255)
                                                                                    gtgtTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGACCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCg                                                                   >  1:715114/1‑150 (MQ=255)
                                                                                     tgtTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGAAACGCGTCCAGCGCTGTGGTTACTGTTAGTCGCGTTTATCCTGATGCTGGTGTGGCTGGTTCGTTTTCACGGAGGAATTGACAGTATGGt                                                                  >  1:418380/1‑150 (MQ=255)
                                                                                      gtTATGAAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTGGCAAAATTTGGCATCGGGTCCCGCGCTGTGGATACTGTTTGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTGGTTAACCCCGACGAAATGACAGTATCGTg                                                                 <  2:950704/150‑1 (MQ=255)
                                                                                        tATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGAc                                                               <  1:1393201/150‑1 (MQ=255)
                                                                                         aTGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCt                                                                                                            <  2:1649377/104‑1 (MQ=255)
                                                                                         aTGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCt                                                                                                            >  1:1649377/1‑104 (MQ=255)
                                                                                          tGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaa                                                             <  2:1476629/150‑1 (MQ=255)
                                                                                           gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                            >  2:1673570/1‑150 (MQ=255)
                                                                                           gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                            >  1:1330841/1‑150 (MQ=255)
                                                                                           gCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCGGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaat                                                            <  2:1534935/150‑1 (MQ=255)
                                                                                              aCCGCTGCTAAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGAGCTGTTGATACTGTTAGTCGCGATTATCCTGATGCTCGTCTGGCTCGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaataat                                                         >  2:1560633/1‑150 (MQ=255)
                                                                                              aCCGCTCCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaataat                                                         >  1:148132/1‑150 (MQ=255)
                                                                                                cGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAc                                                       <  1:441582/150‑1 (MQ=255)
                                                                                                  ctgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAccc                                                     <  2:560913/150‑1 (MQ=255)
                                                                                                   tgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCa                                                    <  2:497195/150‑1 (MQ=255)
                                                                                                    gcgtAATCCTTTTCCACCGATGATCGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGGCTGGCTGGCTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAg                                                   <  2:1447547/147‑1 (MQ=255)
                                                                                                        aaTCCTTTTCCACCGATGATTGACACGTTGCAAAATATGGCAACGGGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCAcc                                               <  2:291358/150‑1 (MQ=255)
                                                                                                          tCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGc                                             >  1:779665/1‑150 (MQ=255)
                                                                                                             ttttCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCaga                                          >  1:64924/1‑150 (MQ=255)
                                                                                                                  cACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGTAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCg                                     >  2:227271/1‑150 (MQ=255)
                                                                                                                    ccGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGcc                                   <  1:1557548/150‑1 (MQ=255)
                                                                                                                     cGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGccc                                  <  2:1570696/150‑1 (MQ=255)
                                                                                                                           tgGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGCCAATAAGACCCAGCACCGCCGAGATCGCCCCGGCaa                            >  2:930834/1‑150 (MQ=255)
                                                                                                                            ggACGCGTTGCCATATTTTGCAACGTGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTACCCCCGACGAAATGACAGTATCGTGACAATAATACCCCGCACCGCAGACATCGCCCCGGCAAg                           >  2:1242592/1‑150 (MQ=255)
                                                                                                                             gACGCGTTGCCATATTTTGCAACGTGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGa                          >  1:306598/1‑150 (MQ=255)
                                                                                                                               cgcGTTGCCATATTTTGCAACGTGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGaaa                        >  1:678909/3‑150 (MQ=255)
                                                                                                                               cacGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGaaa                        >  1:1338480/1‑150 (MQ=255)
                                                                                                                               cacGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGaaa                        >  2:1585642/1‑150 (MQ=255)
                                                                                                                                 cGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATa                      >  1:746615/1‑150 (MQ=255)
                                                                                                                                      cAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGaa                 >  2:1101672/1‑150 (MQ=255)
                                                                                                                                       aaaaTATGGCAACGCGTCACGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTGTGGCTGGTGCGGACACCCCGACGAAATGACAGTATCGTGACAAAAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATTCCGAag                <  1:1102885/150‑1 (MQ=255)
                                                                                                                                        aaaTATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAaga               >  1:518680/1‑150 (MQ=255)
                                                                                                                                         aaTCTGTCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCTATTATCCTGATGTTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAagag              <  2:1702829/150‑1 (MQ=255)
                                                                                                                                         aaTATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAagag              >  2:218827/1‑150 (MQ=255)
                                                                                                                                                    gcgTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAGTAACCAAACGt   <  1:1585642/150‑1 (MQ=255)
                                                                                                                                                     cgTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAGTAACCAAACGTg  <  2:625601/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAGTAACCAAACGTG  >  NC_000913/2438734‑2439032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: