Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,406,129 T→C intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,406,1290TC99.2% 408.4 / NA 126intergenic (+144/‑61)purA/nsrRadenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (64/60);  minor base A (1/0);  total (66/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

ATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAT  >  NC_000913/4405985‑4406272
                                                                                                                                                |                                                                                                                                               
aTTCTGGTACGCCTGGCAGATATATTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCtg                                                                                                                                               >  1:1075625/1‑147 (MQ=255)
     ggTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCt                                                                                                                                          <  1:1118470/147‑1 (MQ=255)
       tACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTgg                                                                                                                                        <  1:574709/147‑1 (MQ=255)
        aCGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGt                                                                                                                                       >  2:128162/1‑147 (MQ=255)
        aCGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGt                                                                                                                                       <  1:642626/147‑1 (MQ=255)
              ggCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatca                                                                                                                                 <  2:479610/147‑1 (MQ=255)
              ggCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTGTatca                                                                                                                                 >  2:624301/1‑147 (MQ=255)
                  gATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTTAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTACTGCCCAGAACTGAAATAAATTAGCGACACCGCCTGTGGCTGGTTTATCAGCaa                                                                                                                             >  2:209794/1‑147 (MQ=255)
                   atatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAat                                                                                                                            <  2:165178/147‑1 (MQ=255)
                     atTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTACATCCTCGCTTTTTTCCTTCCCCGACCTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAatat                                                                                                                          >  2:603163/1‑147 (MQ=255)
                     atTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAatat                                                                                                                          <  1:9313/147‑1 (MQ=255)
                     atTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAatat                                                                                                                          >  1:1261889/1‑147 (MQ=255)
                       tttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaa                                                                                                                        <  2:616455/147‑1 (MQ=255)
                        ttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaaa                                                                                                                       >  1:638958/1‑147 (MQ=255)
                           cctgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGt                                                                                                                    <  1:1014160/147‑1 (MQ=255)
                             tgccGGGCGAACAGTGTGATACATTGATGTGTCGGGTAAGCCATTACGCTATACGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAt                                                                                                                  <  2:458118/147‑1 (MQ=255)
                              gccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCAGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGACCTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAtt                                                                                                                 >  2:890014/1‑147 (MQ=255)
                               ccGGGCGTACAGTTTGAGACATTGCTGTGTCGGGTAAGCCATTACGCTATCAGACACAGTGTTATATCCTCGCTTTTTTCCTTCCACGAAATTAAATAAATTAGAGACACCTCCTTTGGCTGGTTTATCATCAATATAAATGTAttt                                                                                                                <  1:125596/147‑1 (MQ=255)
                                 ggtcGAACAGTGTGATACATTGCTGTGTCTGGTAATCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAttttt                                                                                                              <  2:117916/144‑1 (MQ=255)
                                 gggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAttttt                                                                                                              <  2:1120215/147‑1 (MQ=255)
                                 gggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAttttt                                                                                                              <  1:445342/147‑1 (MQ=255)
                                 gggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAttttt                                                                                                              <  2:176022/147‑1 (MQ=255)
                                 gggCGAACAGTGTGATACATTGCTGTGGCGGGTAAGCCATTACGCTATCCGACAGAGTGTTAAATCCTCGCTTTTTTCCTTCCCCTAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAttttt                                                                                                              <  1:54138/147‑1 (MQ=255)
                                  ggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAtttttt                                                                                                             <  2:1247057/147‑1 (MQ=255)
                                   gCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAtttttta                                                                                                            >  1:801354/1‑146 (MQ=255)
                                    cGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTcc                                                                                                           >  1:379060/1‑147 (MQ=255)
                                      aaCAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCg                                                                                                         <  1:1262749/147‑1 (MQ=255)
                                      aaCAGTGTGATACAATGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAAACCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTATCCCg                                                                                                         <  2:480542/147‑1 (MQ=255)
                                       aCAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGa                                                                                                        >  1:157168/1‑147 (MQ=255)
                                         aGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAtt                                                                                                      >  2:560043/1‑147 (MQ=255)
                                           tgtgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTc                                                                                                    <  1:1271718/147‑1 (MQ=255)
                                            gtgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTcc                                                                                                   >  2:1295635/1‑147 (MQ=255)
                                             tgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                                                                  >  1:7750/1‑147 (MQ=255)
                                               aTACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCGGATTTCCGtt                                                                                                >  1:380499/1‑147 (MQ=255)
                                                  cATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTg                                                                                             >  2:33018/1‑147 (MQ=255)
                                                   aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGa                                                                                            <  2:810608/147‑1 (MQ=255)
                                                   aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGa                                                                                            <  2:522970/147‑1 (MQ=255)
                                                       cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGtt                                                                                        <  1:1225699/147‑1 (MQ=255)
                                                            tCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCAGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTGCCGATTTCCCTTTTGAGGTTGAtgt                                                                                   >  2:30515/1‑147 (MQ=255)
                                                             cGGGTTAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                                  <  2:1143418/147‑1 (MQ=255)
                                                             cGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                                  <  1:495201/147‑1 (MQ=255)
                                                             cGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                                  <  1:1178013/147‑1 (MQ=255)
                                                                  aaGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGtt                                                                             <  2:863099/147‑1 (MQ=255)
                                                                  aaGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGtt                                                                             <  1:1094136/147‑1 (MQ=255)
                                                                   aGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTa                                                                            <  2:19461/147‑1 (MQ=255)
                                                                       aTTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGTACTGAAATAAATTTGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGa                                                                        <  1:133268/147‑1 (MQ=255)
                                                                       aTTACGCGATCCGAAACAGTGTAAAATCGTCGCTTTATTCATTCCCCAAACTGAAAAAAATTAGCGACACAGCATGTCCCTGGTTTATCATCAATATAACTGTATTTTTTCCCTCTTTCCCAGTTGAGGTTGCTGTGCAGTTAACGa                                                                        >  1:220800/1‑147 (MQ=255)
                                                                        ttACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAg                                                                       >  2:58055/1‑147 (MQ=255)
                                                                            gCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTc                                                                   <  1:728359/147‑1 (MQ=255)
                                                                             cTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGTCACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCa                                                                  <  2:790765/147‑1 (MQ=255)
                                                                               aTCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACt                                                                <  1:304590/147‑1 (MQ=255)
                                                                                tCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTAGAGGTTTATGTGCAGTTAACGAGTTTCACTg                                                               >  2:175886/1‑147 (MQ=255)
                                                                                tCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCACGATTTACCTTTTGAGGTTGATGTGAAGTTAACGAGTTTCACTg                                                               >  1:1143708/1‑147 (MQ=255)
                                                                                    acacAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatta                                                           <  2:1111538/147‑1 (MQ=255)
                                                                                     cacaGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattac                                                          >  1:271082/1‑147 (MQ=255)
                                                                                     cacaGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattac                                                          >  2:10430/1‑147 (MQ=255)
                                                                                      acaGTGTTAAATCCTCGCTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacgg                                                        >  2:630638/1‑147 (MQ=255)
                                                                                          tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatt                                                     >  2:937756/1‑147 (MQ=255)
                                                                                          tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatt                                                     >  1:84533/1‑147 (MQ=255)
                                                                                          tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAATAGTTTCACTaattacggatt                                                     >  2:552381/1‑147 (MQ=255)
                                                                                              aaaTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGttt                                                                    >  2:456047/1‑128 (MQ=255)
                                                                                              aaaTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGttt                                                                    <  1:456047/128‑1 (MQ=255)
                                                                                              aaaTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                 >  1:947594/1‑147 (MQ=255)
                                                                                              aaaTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                 >  1:916456/1‑147 (MQ=255)
                                                                                              aaaTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                 >  1:837228/1‑147 (MQ=255)
                                                                                                    tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTg                                           <  1:610659/147‑1 (MQ=255)
                                                                                                     cGCTTTTTTCCTTCCCCGAACTGAATTAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTAAGGATTACGTGCGCTGa                                          >  2:713846/1‑147 (MQ=255)
                                                                                                      gCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCGTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                         >  2:544815/1‑147 (MQ=255)
                                                                                                      gCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                         >  1:1191989/1‑147 (MQ=255)
                                                                                                      gCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGAGTACGTGCGCTGAt                                         >  2:892661/1‑147 (MQ=255)
                                                                                                        ttttttCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCTATTTAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTATCGAGTTTCACTGATTACGGATTACGTGCGCTGATCt                                       <  1:679157/147‑1 (MQ=255)
                                                                                                             tccttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATg                                  <  2:1177474/147‑1 (MQ=255)
                                                                                                              ccttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAAGATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATgg                                 >  2:729946/1‑147 (MQ=255)
                                                                                                                 tccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGt                              >  1:87192/1‑147 (MQ=255)
                                                                                                                  ccccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACTGATTACGTGCGCTGATCTACATGGCGTc                             >  2:779134/1‑147 (MQ=255)
                                                                                                                  ccccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTc                             >  2:1231866/1‑147 (MQ=255)
                                                                                                                  ccccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTAAGGATTACGTGCGCTGATCTACATGGCGTc                             >  1:788732/1‑147 (MQ=255)
                                                                                                                   cccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCa                            <  1:465520/147‑1 (MQ=255)
                                                                                                                      gAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTg                         >  2:279936/1‑147 (MQ=255)
                                                                                                                             aataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAg                  >  1:423620/1‑147 (MQ=255)
                                                                                                                              ataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTAGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAgg                 >  1:209548/1‑147 (MQ=255)
                                                                                                                                aaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc               >  1:322809/1‑147 (MQ=255)
                                                                                                                                aaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc               >  2:663061/1‑147 (MQ=255)
                                                                                                                                 aatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCg              <  1:226813/147‑1 (MQ=255)
                                                                                                                                 aatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCg              >  2:805125/1‑147 (MQ=255)
                                                                                                                                 aatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCCTCATTGCCAGAAGGGCg              >  2:1175678/1‑147 (MQ=255)
                                                                                                                                  atTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCgg             <  2:638958/147‑1 (MQ=255)
                                                                                                                                   ttAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGTCGTCATTGCCAGAAGGGCGGa            >  2:235719/1‑147 (MQ=255)
                                                                                                                                   ttAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGa            >  1:548013/1‑147 (MQ=255)
                                                                                                                                     aGCGACACAGCCTGTGGCTGGTTTATCATCAATCTAAATGTATTGTTTCCCGCTTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACTGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATg          <  2:212164/147‑1 (MQ=255)
                                                                                                                                     aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATg          >  2:300984/1‑147 (MQ=255)
                                                                                                                                     aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATg          <  2:95468/147‑1 (MQ=255)
                                                                                                                                     aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATg          >  2:1173189/1‑147 (MQ=255)
                                                                                                                                     aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAGTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATg          <  1:1027725/147‑1 (MQ=255)
                                                                                                                                     aGCGACACAGCCTGAGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATg          >  2:352799/1‑147 (MQ=255)
                                                                                                                                         acacAGCCTGTGGCTGGTTTATCATCAATATAATTGTATTTTTTCCCGATTACCCTTTTGAGGTTGATGAGCAGTTAACCAGTTTCACTGATTACGGAATACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCa      >  2:703400/1‑147 (MQ=255)
                                                                                                                                           acaGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGt    >  2:962867/1‑147 (MQ=255)
                                                                                                                                            caGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTa   <  2:87192/147‑1 (MQ=255)
                                                                                                                                             aGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAt  >  2:521219/1‑147 (MQ=255)
                                                                                                                                             aGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTACACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGACAGAAGGGCGGATGACCAGTAt  >  1:712270/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAT  >  NC_000913/4405985‑4406272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: