Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,185,540	C→T	P64L (CCG→CTG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,185,540	0	C	T	100.0%	292.4 / NA	94	P64L (CCG→CTG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (55/39);  total (55/39)

TTGATGCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCG  >  NC_000913/4185399‑4185679                                                                                                                                              |                                                                                                                                            ttGATGCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCTTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTaa                                                                                                                                        <  1:229416/147‑1 (MQ=255)  tGATGCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTaaa                                                                                                                                       >  2:463994/1‑147 (MQ=255)  tGATGCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTaaa                                                                                                                                       >  2:608796/1‑147 (MQ=255)     tgagATCAAAATTGCTCTGGCTTCTCCAGACATGATCCGTTCATGGTCTTTCGGTTAAGTTAAAAAGCCGTAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGa                                                                                                                                    <  1:1093524/144‑1 (MQ=255)      gCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGAt                                                                                                                                   <  2:8251/147‑1 (MQ=255)       cGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTTAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCACGTTTCTTTGGGCTGGTAAAAGAtt                                                                                                                                  >  1:657410/1‑147 (MQ=255)       cGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGAtt                                                                                                                                  <  2:34162/147‑1 (MQ=255)          tCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGTAGTTAAAAAGCCGGAACCCATCAACTACCGTACGTTGAAACCAGAACGTGCCGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACg                                                                                                                               >  1:621317/1‑147 (MQ=255)          tCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACg                                                                                                                               >  2:608497/1‑147 (MQ=255)          tCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACg                                                                                                                               >  2:675603/1‑147 (MQ=255)           cAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGa                                                                                                                              >  1:1180629/1‑147 (MQ=255)            aaaaTTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAg                                                                                                                             >  1:1087910/1‑147 (MQ=255)             aaaTTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTAAGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGt                                                                                                                            >  1:366649/1‑147 (MQ=255)                   ctctGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCtgt                                                                                                                      <  2:10437/147‑1 (MQ=255)                    tctGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCtgtg                                                                                                                     <  2:527711/147‑1 (MQ=255)                      tGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCg                                                                                                                   <  2:904971/147‑1 (MQ=255)                      tGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCg                                                                                                                   <  1:1081303/147‑1 (MQ=255)                           tctccAGACTTGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAg                                                                                                              <  2:16789/144‑1 (MQ=255)                            cGCAAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGGAAGt                                                                                                             >  2:499672/1‑147 (MQ=255)                                aGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACaa                                                                                                         <  2:1087910/147‑1 (MQ=255)                                  aCATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAgc                                                                                                       >  2:875184/1‑147 (MQ=255)                                   cATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAAACATCAACTACAGTACGTTCAAACCAGAACTTGACGGCCTTTTCTGCGCCCGTATCTTTCGGCTGGTAAAAGATTACGAGTGCCTGTGCGCTAAGTACAAtcg                                                                                                      >  2:913270/1‑147 (MQ=255)                                   aaTGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAgcg                                                                                                      <  2:520420/146‑1 (MQ=255)                                     tGAACCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCTTACTTTCAAACCAGAACGTGACGGCCTTTTCTGAGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCCGTAAGTACAAGCGcc                                                                                                    >  2:801883/1‑147 (MQ=255)                                        tCCGTTCATGGTCTTTCTGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGa                                                                                                 <  1:70425/147‑1 (MQ=255)                                        tCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGa                                                                                                 >  2:806855/1‑147 (MQ=255)                                        tCCGTTCATGGGCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGa                                                                                                 <  1:101840/147‑1 (MQ=255)                                         ccGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTGTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGaa                                                                                                >  2:1224559/1‑147 (MQ=255)                                         ccGTTAATGGTAGTTCGGTGAATTTAAAAATCCGGATACCATCAACAACCGTAAGTTAAAACCAGAACGTCAATGCCTGATCTGAGACCGGATCTTGGGGCTGGTAAAACATTACCAGTGCCTGTGCGGTAAGTACAAGCGCCTgca                                                                                                >  2:326981/1‑145 (MQ=255)                                          cGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGaaa                                                                                               >  2:1162232/1‑147 (MQ=255)                                          cGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGaaa                                                                                               >  2:314545/1‑147 (MQ=255)                                              cATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACAcc                                                                                           <  2:585081/147‑1 (MQ=255)                                                 ggTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTGTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTg                                                                                        >  2:379194/1‑147 (MQ=255)                                                      ttCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAGCACCGTGGCGTc                                                                                   <  1:1276848/147‑1 (MQ=255)                                                      ttCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTc                                                                                   <  2:15414/147‑1 (MQ=255)                                                       tCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCACACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACCAGCGCCTGAAACACCGTGGCGTCa                                                                                  >  2:803085/1‑147 (MQ=255)                                                           tGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGACACACCGTGGCGTCATCtg                                                                              >  1:246763/1‑147 (MQ=255)                                                            gAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCtgt                                                                             >  1:583253/1‑147 (MQ=255)                                                            gAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCtgt                                                                             <  2:751990/147‑1 (MQ=255)                                                            gAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGCCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCAGCtgt                                                                             >  1:232704/1‑147 (MQ=255)                                                            gAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGACTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCtgt                                                                             <  1:1003629/147‑1 (MQ=255)                                                               tttAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTgag                                                                          <  2:682919/146‑1 (MQ=255)                                                               gTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTgag                                                                          <  1:486393/147‑1 (MQ=255)                                                                 tAAATAGCCGGAAACCATCAACTACCGTTCGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGACTGCCTGTGCTTTAAGTACAAGCGCCTGAAACACCGTGTCGTCATCTGTGAGaa                                                                        <  2:608315/147‑1 (MQ=255)                                                                  aaaaaGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAg                                                                       >  2:22023/1‑147 (MQ=255)                                                                  aaaaaGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAg                                                                       >  2:1124341/1‑147 (MQ=255)                                                                   aaaaGCCGTAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTTGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGt                                                                      <  2:497856/147‑1 (MQ=255)                                                                    aacgCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGGATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTGCAAGCGCCTGAAACACCGTGGCGTCAGCGCTGAGAAGTg                                                                     >  1:94422/4‑147 (MQ=255)                                                                       gCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTgcgg                                                                  >  1:317104/1‑147 (MQ=255)                                                                            aaaCCATCAACTAGCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTACAAGATTACTAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGGCATCTGTGAGAAGTGCGGCGTTg                                                             <  1:666856/147‑1 (MQ=255)                                                                            aaaCCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTg                                                             <  1:94727/147‑1 (MQ=255)                                                                            aaaCCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTg                                                             <  1:806855/147‑1 (MQ=255)                                                                                  tCAACTACCGTACGTTCAAACCAGAACGTGACGGGCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTgc                                                       >  1:519087/1‑146 (MQ=255)                                                                                  tCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAGACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGa                                                       >  1:1260009/1‑147 (MQ=255)                                                                                  tCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGa                                                       <  1:1006175/147‑1 (MQ=255)                                                                                  tCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGa                                                       >  1:837430/1‑147 (MQ=255)                                                                                   cAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGAc                                                      >  1:882994/1‑147 (MQ=255)                                                                                   cAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTAAAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGTCGTTGAAGTGAc                                                      >  1:834913/1‑147 (MQ=255)                                                                                   cAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTGTGGGCTGGTAAAAGATTACGAGTGCCTGAGCGGTAAGTACAAGCGCCTGATACACGGTCGCGTCATCTGTGAGAACTCCGGCGTTGAAGTGAc                                                      >  2:1188672/1‑147 (MQ=255)                                                                                    aaCTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGAcc                                                     <  1:1193833/147‑1 (MQ=255)                                                                                    aaCTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGAcc                                                     >  1:561601/1‑147 (MQ=255)                                                                                      cTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCa                                                   <  1:1030758/147‑1 (MQ=255)                                                                                      cTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGATGGTAAAAGAATACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATATGTGAGAAGTGCGGCGTTGAAGTGAcaca                                                   >  2:862325/1‑147 (MQ=255)                                                                                       tACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAg                                                  >  2:830811/1‑147 (MQ=255)                                                                                          cGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACt                                               <  2:754523/147‑1 (MQ=255)                                                                                            tACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTTTCTTTGGGCTGGTAATAGATTTCGTGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTaa                                             <  1:97021/147‑1 (MQ=255)                                                                                                 tCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTAc                                        >  1:918671/1‑147 (MQ=255)                                                                                                  cAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACg                                       >  1:263765/1‑147 (MQ=255)                                                                                                  cAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACg                                       >  2:604379/1‑147 (MQ=255)                                                                                                  cAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGAAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAGGTGACCCAGACTAAAGTACg                                       >  1:345583/1‑147 (MQ=255)                                                                                                         gAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGATACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAg                                >  1:242217/1‑147 (MQ=255)                                                                                                           aCGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCg                              >  1:943134/1‑147 (MQ=255)                                                                                                           aCGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCg                              >  1:104278/1‑147 (MQ=255)                                                                                                              tGACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTAt                           <  2:493572/147‑1 (MQ=255)                                                                                                              ggACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCGGTGCGGTAAGTACAAGCGCCGGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGGGACCCAGACTAAAGGACGCCGTGAGCGTAt                           <  1:1180326/146‑1 (MQ=255)                                                                                                               gACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATg                          >  1:801369/1‑147 (MQ=255)                                                                                                               gACGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATAACGCGTGCCTGTGCCGTACGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGACGTGCGGCGTAGAAGTGACCCAGACTAAAGTACGCAGTGAGAGTATg                          >  1:190319/1‑147 (MQ=255)                                                                                                                aCGGCCTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATgg                         <  1:1126226/147‑1 (MQ=255)                                                                                                                    ccTTTTCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCca                     >  2:306907/1‑147 (MQ=255)                                                                                                                      ttttCTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCcaca                   >  2:415351/1‑147 (MQ=255)                                                                                                                        tacTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATc                 >  2:755610/3‑147 (MQ=255)                                                                                                                          ttGCGCCCATATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGa               <  2:1054460/146‑1 (MQ=255)                                                                                                                          cTGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGa               <  1:1243685/147‑1 (MQ=255)                                                                                                                           tGCGCCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGCAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGaa              <  1:23704/147‑1 (MQ=255)                                                                                                                            gctcccGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCGGTGCGGTAAGTAAACGCGCCTGACCCACCGTGGCGTCATCTGTGAGAATTGCGGCGTTGAAGTGACCCAGCCTAAATTACGCCGTTAGCTTATGGGCCACATCTAAc             <  1:1109564/144‑1 (MQ=255)                                                                                                                            gcgcCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAAc             <  2:1191226/147‑1 (MQ=255)                                                                                                                             cgcCCGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACt            >  2:1257609/1‑147 (MQ=255)                                                                                                                                 cGTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCt        >  2:719130/1‑147 (MQ=255)                                                                                                                                  gTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTATGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCtt       >  2:461724/1‑147 (MQ=255)                                                                                                                                  gTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCtt       >  2:431250/1‑147 (MQ=255)                                                                                                                                  gTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCtt       >  1:971900/1‑147 (MQ=255)                                                                                                                                  gTATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACAGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGAATAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCtt       >  1:90038/1‑147 (MQ=255)                                                                                                                                   tATCTTTGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTc      <  1:389220/147‑1 (MQ=255)                                                                                                                                       tttGGGCTGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCg  >  1:224463/1‑147 (MQ=255)                                                                                                                                              |                                                                                                                                            TTGATGCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCG  >  NC_000913/4185399‑4185679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑