Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,131 T→G 6.6% intergenic (+114/‑84) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1310TG 6.6% 1232.7 / 36.4 372intergenic (+114/‑84)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (269/61);  new base G (10/16);  total (292/80)
Fisher's exact test for biased strand distribution p-value = 4.96e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 381 total aligned reads displayed.

CACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGC  >  NC_000913/3780983‑3781280
                                                                                                                                                     |                                                                                                                                                       
  cTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCg                                                                                                                                                      <  2:4925532/150‑1 (MQ=255)
  cTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCg                                                                                                                                                      <  2:5855805/150‑1 (MQ=255)
  cTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGAACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCg                                                                                                                                                      <  2:7376361/150‑1 (MQ=255)
   tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                                                                     <  1:405460/150‑1 (MQ=255)
   tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACACCCACAGGGAGAGGGGGCCCTTCGGCGCTGTATGTACTCCCACACACAGAAACGACACCCCACACTTTTTTTCTCCCACGCCCCACCgg                                                                                                                                                     >  2:7564110/1‑150 (MQ=255)
    ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                    <  1:7944189/150‑1 (MQ=255)
    ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                    <  1:6380116/150‑1 (MQ=255)
    ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                    <  1:4288126/150‑1 (MQ=255)
    ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGGGCGCCCCCCCCCCCCAACCCACGCCCCCAGGGCGAGGGGCCCCGGCGGCGCGGGGGGTACTCCCCCACCCGGAAACGACACCCCCCGCGTTTTTTCGCCCGCGCCCCACCgcg                                                                                                                                                    >  2:4127944/1‑148 (MQ=255)
          cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgg                                                                                                                                              <  1:2825649/150‑1 (MQ=255)
          cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgg                                                                                                                                              <  1:2096735/150‑1 (MQ=255)
           aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                                                                             <  2:1032414/150‑1 (MQ=255)
           aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                                                             <  2:7491801/150‑1 (MQ=255)
            tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggga                                                                                                                                            <  2:5181745/150‑2 (MQ=255)
            tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACACTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggga                                                                                                                                            <  1:6248248/150‑2 (MQ=255)
              gCGAAAGGGAATGCGGCATAGTCTTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATTTACTCCCTCACTATTAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                                                                                          <  1:1016340/150‑1 (MQ=255)
               cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgggagaa                                                                                                                                         <  1:767752/150‑5 (MQ=255)
               cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                                                         <  2:8765926/150‑1 (MQ=255)
                gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACACCCACAGGGAGAGGGGACCCTTCCGCGCGGTATGTACTCCCGCACAATGAAAACCCCCCCCACACTTTTTTTATACCGCGCCCCGCCCGGGCGGGGGCCggg                                                                                                                                        >  2:1173610/1‑150 (MQ=255)
                    gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                                                    <  2:7057531/150‑1 (MQ=255)
                     ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                                                   <  2:2524807/150‑1 (MQ=255)
                      gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                  <  1:5716672/150‑1 (MQ=255)
                                   tCGTTTGCGCCCCCTCACCCTAACCCAATACCTCAGGGAGAGGGGACCCTTAGGCGCTGTATGTACGCCCGCACTCTGAAAACACACCCCACTCTTTTTTTCTCACACGCCCCGCCGGGGCGAGGGCCCGGGTGATGGG‑‑AAAAGGCCGCa                                                                                                                   >  2:6844202/1‑150 (MQ=255)
                                   tCGTTTGCGCCCCCTCACCCTAAACCAATACCACAGGGAGAGGGGACCGTTCGGCGCTGTATTTACTCCCTCACTATGAAACGACACCGCACTCTTTTTTTATACCACGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAATGCCCCa                                                                                                                   >  2:2150465/1‑150 (MQ=255)
                                   tCGTTTGCGCCCCCACACCCAAACCCACACCCACAGGGAGAGGGGACCGTTCGGCGCTGTATGTAATCCCTCACACTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCa                                                                                                                   >  2:5480302/1‑150 (MQ=255)
                                      tttGCGCCCCCTCACCCAAAACCACACCCACAGGGAGAGGGGACCCTTCGGCGCTGTATGTACTACCGCACTCGGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCCGGGAGAGGGCCGGGGGGGGGGG‑‑AAAAGGCCGCACTg                                                                                                                >  2:6017207/1‑150 (MQ=255)
                                           gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCAc                                                                                                           >  1:9550842/1‑150 (MQ=255)
                                           gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCAc                                                                                                           >  1:4505397/1‑150 (MQ=255)
                                           gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCAc                                                                                                           >  1:6874283/1‑150 (MQ=255)
                                             ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCa                                                                                                         >  2:9776140/1‑150 (MQ=255)
                                               ccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAAt                                                                                                       >  2:3907635/1‑150 (MQ=255)
                                               ccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTGTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAAt                                                                                                       >  2:4605202/1‑150 (MQ=255)
                                                cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑ACAAGGCCGCACTGCTCACCAAtt                                                                                                      >  1:8418555/1‑150 (MQ=255)
                                                 tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAAttt                                                                                                     >  2:1857670/1‑150 (MQ=255)
                                                      cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                                                >  1:3248228/1‑150 (MQ=255)
                                                        aaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTa                                                                                              >  1:2919187/1‑150 (MQ=255)
                                                           ccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                           >  1:5312717/1‑150 (MQ=255)
                                                           ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTCTGTACTCCCTCACTCTGAAACGCCACCGCACTCTTGTGTTCTCCCTCGCCCCTCCGGGGAGCGGGCCGGGGTGAGGGG‑‑AACAGGCCGCACTGCTCACCAATTTGCTGCTATcc                                                                                           >  2:3623402/1‑150 (MQ=255)
                                                           ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAATCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                           >  2:8960422/1‑150 (MQ=255)
                                                           ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                           >  1:8523749/1‑150 (MQ=255)
                                                           ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                           >  2:2427861/1‑150 (MQ=255)
                                                           ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCACACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                           >  1:8949140/1‑150 (MQ=255)
                                                            ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                          >  1:8759688/1‑150 (MQ=255)
                                                            ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                          >  1:4828327/1‑150 (MQ=255)
                                                             tctcCCTGAGGGAGAGGGTACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                         >  2:381524/1‑150 (MQ=255)
                                                             tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                         >  2:2214127/1‑150 (MQ=255)
                                                             tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                         >  1:5965418/1‑150 (MQ=255)
                                                               tcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc                                                                                       >  1:2156538/1‑150 (MQ=255)
                                                                 ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                                                     >  1:5126341/1‑150 (MQ=255)
                                                                 ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                                                     >  2:9646481/1‑150 (MQ=255)
                                                                        gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                                              >  1:1257146/1‑150 (MQ=255)
                                                                           gggggAGCGTGCGGCGGGTTATGTACCCCCTCACTCCGACCCGACCCCGCAGTCTTTTTTCCGCCCGCGCCCCGGGGGGGCGGGGGGGGGGGTGGGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                           <  2:1485305/149‑1 (MQ=255)
                                                                             gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                         >  1:982852/1‑150 (MQ=255)
                                                                             gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                         >  1:9184088/1‑150 (MQ=255)
                                                                             gggACCGTTCGGCGCTGAATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCCCTAAgggg                                                                         >  2:4253717/1‑150 (MQ=255)
                                                                               gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                                                       >  2:4763977/1‑150 (MQ=255)
                                                                                aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                                                                      >  2:8574307/1‑150 (MQ=255)
                                                                                  cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                    >  2:2201432/1‑150 (MQ=255)
                                                                                  cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                    >  1:2678166/1‑150 (MQ=255)
                                                                                   gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                                                   >  2:2949789/1‑150 (MQ=255)
                                                                                         cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                                                             >  2:7894678/1‑150 (MQ=255)
                                                                                          gcTGTTTGCACCCCCCCACCCTCAAACCCCCCCCCACTATTTTTTCCCCCCCCCCCCCCCGGGGTTGCGGGGGGGGGTGCGGCC‑‑ACAAGGCCGCACTGCTCACCAATTTTCTGCTATACTGCCCCCTTAGTGCGGGGGCAGGAtagcaga                                                            <  2:6363662/150‑7 (MQ=255)
                                                                                          gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                                                            >  2:2068687/1‑150 (MQ=255)
                                                                                           cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAAc                                                           >  1:6656398/1‑150 (MQ=255)
                                                                                             gtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt                                                         >  2:2469007/1‑150 (MQ=255)
                                                                                                 gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                                                     >  2:7729766/1‑150 (MQ=255)
                                                                                                  taCTCCCTGACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                                                    >  1:1799581/1‑150 (MQ=255)
                                                                                                       ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                               >  2:7175216/1‑150 (MQ=255)
                                                                                                         tCACTCTGAAACGACACCGCACTCTTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                                              >  1:4076658/1‑150 (MQ=255)
                                                                                                         tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                             >  1:9918950/1‑150 (MQ=255)
                                                                                                         tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                             >  1:8286929/1‑150 (MQ=255)
                                                                                                                 aaaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa                                     >  2:5432540/1‑150 (MQ=255)
                                                                                                                     gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACGAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc                                 >  2:9185535/1‑150 (MQ=255)
                                                                                                                        acCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGcc                              >  2:8381743/1‑150 (MQ=255)
                                                                                                                        acCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGcc                              >  2:1725780/1‑150 (MQ=255)
                                                                                                                         ccGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCa                             >  1:724220/1‑150 (MQ=255)
                                                                                                                          cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                            >  1:7491384/1‑150 (MQ=255)
                                                                                                                              cacgtttttttCACCCCCGCCCCGCGGGGGGGGGGGGGGGGGGGGGGGGCCCCAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaaa                           <  2:3145274/146‑1 (MQ=255)
                                                                                                                               tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCCGAAATTCCGCCAAATACt                       >  2:6906044/1‑150 (MQ=255)
                                                                                                                               tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                       >  1:2566330/1‑150 (MQ=255)
                                                                                                                               tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                       >  1:8851903/1‑150 (MQ=255)
                                                                                                                                ctTTTTTCCCCCCCCCCCCCGCGGGGGAGCGGGCGGGGGTGGGGGC‑‑ACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg                      <  2:8298961/150‑1 (MQ=255)
                                                                                                                                  tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     >  1:1040318/1‑150 (MQ=255)
                                                                                                                                  tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     >  1:2353210/1‑150 (MQ=255)
                                                                                                                                  tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     >  1:2482738/1‑150 (MQ=255)
                                                                                                                                  ttttttcCTCCCGCCCCCCCCGGGGGCGGGGGGGGGGGTGTGGCC‑‑ACAAGGCCGCACTGCCCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     <  2:1414929/150‑1 (MQ=255)
                                                                                                                                       ttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAca                >  2:9303262/1‑150 (MQ=255)
                                                                                                                                          tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca             >  2:1616082/1‑150 (MQ=255)
                                                                                                                                          tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca             >  1:3833626/1‑150 (MQ=255)
                                                                                                                                           cccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat            >  2:9065766/1‑150 (MQ=255)
                                                                                                                                            ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc           >  2:4515148/1‑150 (MQ=255)
                                                                                                                                             cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc          >  1:6749081/1‑150 (MQ=255)
                                                                                                                                             cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc          >  2:2679833/1‑150 (MQ=255)
                                                                                                                                              tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg         >  2:2866747/1‑150 (MQ=255)
                                                                                                                                                   ccTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt    >  1:5366097/1‑150 (MQ=255)
                                                                                                                                                    cTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTg   >  2:163835/1‑150 (MQ=255)
                                                                                                                                                    cTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGACAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTg   >  1:1490380/1‑150 (MQ=255)
                                                                                                                                                     tCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc  >  2:8121273/1‑150 (MQ=255)
                                                                                                                                                     tCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc  >  2:8165994/1‑150 (MQ=255)
                                                                                                                                                     tCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc  >  2:8623934/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
CACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGC  >  NC_000913/3780983‑3781280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: