Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,244,626 T→A 7.3% K150N (AAA→AAT malF ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,6260TA 7.3% 2063.7 / 23.3 665K150N (AAA→AATmalFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (218/377);  new base A (67/2);  total (286/379)
Fisher's exact test for biased strand distribution p-value = 1.59e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 667 total aligned reads displayed.

GCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGT  >  NC_000913/4244479‑4244775
                                                                                                                                                   |                                                                                                                                                     
  tcatcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTAAGTTGCAGTTTTTTCTCGCCGCCAAATTTaa                                                                                                                                                   >  1:1949871/1‑150 (MQ=255)
   catcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaa                                                                                                                                                  <  2:8489701/150‑1 (MQ=255)
   acctataaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTTCAGTTTTTTGTCGCCGCCAAAATTaaa                                                                                                                                                  >  2:4505211/8‑150 (MQ=255)
    atcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaaa                                                                                                                                                 <  2:5140511/150‑1 (MQ=255)
    atcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTATTTGAGTTGCAGGTTTTGCTCGCCGCCCAAATTaaaa                                                                                                                                                 >  1:2777318/1‑150 (MQ=255)
     tcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTCAGTTGCAGTTTTTTCTCGCCGCCCAAATTAAAAg                                                                                                                                                >  1:3114628/1‑150 (MQ=255)
        tcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGt                                                                                                                                             >  1:4899706/1‑150 (MQ=255)
            tttGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGga                                                                                                                                         >  1:9735146/1‑150 (MQ=255)
                 tgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGTTGCAGTGTTTGCTCGCCCCCCAAATTAAAAGCGTCGGAGAGGt                                                                                                                                    >  2:2920307/1‑150 (MQ=255)
                  gCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTTCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTa                                                                                                                                   >  2:113091/1‑150 (MQ=255)
                  gCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTa                                                                                                                                   >  2:1532372/1‑150 (MQ=255)
                   ccATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGGTCGCCTTCGGGCTGGGCGGTCGTGTGGTGGAGGTGCAGATTTTGCTCCCCGCCCAAATTAAAAACGGCGgaccgggaa                                                                                                                                  >  1:1765634/1‑143 (MQ=255)
                    cATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGGTTTTGCTCGCCGCCAAATTTAAAAGAGTCGGAGAGGTAAt                                                                                                                                 >  1:7898141/1‑150 (MQ=255)
                     aTCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGTGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGGTTTTGCTCGCCGCCCAATTTAAAAGCGTCGGAGAGGTCAtt                                                                                                                                >  2:4957456/1‑150 (MQ=255)
                     aTCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAtt                                                                                                                                <  1:8025430/150‑1 (MQ=255)
                      tCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGGTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAttt                                                                                                                               >  2:4877272/1‑150 (MQ=255)
                      tCCGGCAGAATGGCGGTAATGTCACTCAGCACCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTTAGTTTGAGTTTTTGGTGGGCGCCAAATTTAAAAGGGTGGGTGGGGTAAttt                                                                                                                               >  1:5891197/1‑150 (MQ=255)
                        cGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAttttt                                                                                                                             <  2:6032882/150‑1 (MQ=255)
                        cGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTATTTGAGGTTGAGTTTTTGCGGGCCGCCCAAAATAAAAGCGTAGGAGCGGTAAttttt                                                                                                                             >  1:3450978/1‑150 (MQ=255)
                          gCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGc                                                                                                                           <  1:9395997/150‑1 (MQ=255)
                            aGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCg                                                                                                                         <  1:1293867/150‑1 (MQ=255)
                                   cGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAAATTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGc                                                                                                                  <  1:2343057/150‑1 (MQ=255)
                                    ggTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGcc                                                                                                                 <  1:8856625/150‑1 (MQ=255)
                                    ggTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCGTTCAGGTGCAGGTTTTGCTCGCCGCCAAAGTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGcc                                                                                                                 >  2:3883711/1‑150 (MQ=255)
                                            cACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGa                                                                                                         <  1:6881075/150‑1 (MQ=255)
                                             aCTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAg                                                                                                        <  1:9018469/150‑1 (MQ=255)
                                             aCTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCATTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGAGTCGGAGATGTAAATTTTGACGGTTTAGCCGTCGCTGAg                                                                                                        >  2:744086/1‑150 (MQ=255)
                                                  gcgcCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCa                                                                                                   <  1:8171155/150‑1 (MQ=255)
                                                    gcCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTTCCGGGTTAGCCGTCGCTGAGCGCCAGt                                                                                                 >  2:6300542/1‑150 (MQ=255)
                                                      cTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGGTTTTGCTCGCCGCCCCATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGGTTAGCCGGCGCAGCGCGCCAGGTg                                                                                               >  2:4750447/1‑150 (MQ=255)
                                                      cTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTATTTGAGTTGGAGTTTTTTGTCGCCCCCAAAATTAAAAGCGTCGGAGAGGGAAATTTTTGCGGTTTAGCCCTCGCTGAGCGccccttg                                                                                               >  1:3307708/1‑145 (MQ=255)
                                                       tGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCTGTTGc                                                                                              <  2:752625/150‑1 (MQ=255)
                                                       tGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTAAGTTTGAGGTTTTTCTAGCCGCCCAATTTAAAAACGTCGGGGAGGTAAATTTTTGCGGGTTCGCCCGCGCGGAGCGCCACtttc                                                                                              >  1:510150/1‑148 (MQ=255)
                                                       tGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTATTTAAGTTTCAGTTTTTTCTAGCCGCCCAAATTAAAAGAGTAGGAGAGGTAAATTTTTACCGTTTCGCCGGCGCAGAGCGCCCGTTGc                                                                                              >  1:6202996/1‑150 (MQ=255)
                                                         aCGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCa                                                                                            <  1:9218531/150‑1 (MQ=255)
                                                         aCGATTCTGGGTAATCACGCGCAGATTCGCGCGGTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCa                                                                                            <  2:7849903/150‑1 (MQ=255)
                                                          cGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCAc                                                                                           <  1:10028757/150‑1 (MQ=255)
                                                          cGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCAc                                                                                           <  1:4011958/150‑1 (MQ=255)
                                                             ttCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCa                                                                                        <  2:4639831/150‑1 (MQ=255)
                                                             ttCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCGTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGGCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCa                                                                                        >  2:6898197/1‑150 (MQ=255)
                                                               cTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATc                                                                                      <  2:4342335/150‑1 (MQ=255)
                                                               cTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATc                                                                                      <  1:4278015/150‑1 (MQ=255)
                                                                   gTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCAGTCAGTTGCAGGTTTTGCGCGCCGCCAAAAGTAAAAGCCTCGGAGACGTAAGATTTGCCCGGTTCGCCCTCGCAGAGCGCCCGTAGCCACACATcgccc                                                                                  >  2:5564739/1‑150 (MQ=255)
                                                                     aaTCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTATTTCAGTTGGAGTTTTTTGTAGCCCCCAAAATTAAAAGAGTGGGGGAGGGAATTTTTGGCGGGTTGGCCGTCGCTGTGCGCCCCTTTCCACGCAAcgccccc                                                                                >  1:7497668/1‑148 (MQ=255)
                                                                      aTCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcccgcc                                                                               <  2:3423213/150‑1 (MQ=255)
                                                                        cACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCgg                                                                             <  2:1709528/150‑1 (MQ=255)
                                                                        cACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGGTGCAGTTTGTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCgg                                                                             <  2:9734470/150‑1 (MQ=255)
                                                                          cgcgCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGt                                                                           <  2:3649293/150‑1 (MQ=255)
                                                                           gcgcAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGTGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTa                                                                          >  2:8954661/1‑150 (MQ=255)
                                                                            cgcAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGGTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTTCCACTCATCGCCCCCCGGGTaa                                                                         >  2:1230750/1‑150 (MQ=255)
                                                                             gcAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTaaa                                                                        <  2:5759007/150‑1 (MQ=255)
                                                                             gcAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTaaa                                                                        <  2:649775/150‑1 (MQ=255)
                                                                             gcAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTaaa                                                                        <  2:8350110/150‑1 (MQ=255)
                                                                                    cgcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaaa                                                                 <  2:8197644/150‑1 (MQ=255)
                                                                                     gcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTAAGTTGGAGGTTTTTGTCGCCGCCAAAATTAAAATCGTCGGAGTGGTAATTTTTGCGGGTTTAGCCGGCGCTGTGCGCCAGTTTGCACACAACGCCCCCCGGGTAAAGACCAAAg                                                                >  2:3512279/1‑150 (MQ=255)
                                                                                        cgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTta                                                             <  1:7257845/150‑1 (MQ=255)
                                                                                          ttCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGTGTAAAGACCAAAGTtata                                                           <  1:6356352/150‑1 (MQ=255)
                                                                                          ttCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTtata                                                           <  1:3024754/150‑1 (MQ=255)
                                                                                          ttCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCAGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTtata                                                           >  2:8247412/1‑150 (MQ=255)
                                                                                             gCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGt                                                        <  2:6767505/150‑1 (MQ=255)
                                                                                             gCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGt                                                        <  1:7013926/150‑1 (MQ=255)
                                                                                             gCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTATTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGt                                                        <  1:1583349/150‑1 (MQ=255)
                                                                                              ccTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAATCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGtt                                                       >  2:2362032/1‑150 (MQ=255)
                                                                                                  cGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGc                                                   <  1:2664107/150‑1 (MQ=255)
                                                                                                  cGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGc                                                   <  2:5852644/150‑1 (MQ=255)
                                                                                                  cGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGc                                                   <  1:5508776/150‑1 (MQ=255)
                                                                                                     gCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTAGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCAACGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTTCCTg                                                >  2:6673307/1‑150 (MQ=255)
                                                                                                           cGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGcc                                          <  2:8017994/150‑1 (MQ=255)
                                                                                                            ggTCGTTTCTTTCAGTTTCAGTTTTTGCTAGCCGCCAAAATTAAAAGCGTCGGAGAGGTAATTTTTTCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACACATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGACTGCTTTCCa                                         >  2:9513754/1‑150 (MQ=255)
                                                                                                              tCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAgg                                       >  2:4167857/1‑150 (MQ=255)
                                                                                                               cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGa                                      <  2:7670320/150‑1 (MQ=255)
                                                                                                                 tttctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGc                                    >  1:2504100/1‑150 (MQ=255)
                                                                                                                 tttctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTAGCCAGGAGc                                    >  2:2583018/1‑150 (MQ=255)
                                                                                                                  ttctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCg                                   >  1:7585307/1‑150 (MQ=255)
                                                                                                                    ctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGAt                                 <  1:2061812/150‑1 (MQ=255)
                                                                                                                    ctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGAt                                 <  2:8757545/150‑1 (MQ=255)
                                                                                                                     tttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATc                                >  2:9305477/1‑150 (MQ=255)
                                                                                                                     tttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATc                                >  2:3137032/1‑150 (MQ=255)
                                                                                                                      ttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCt                               <  1:3745289/150‑1 (MQ=255)
                                                                                                                      ttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCt                               >  2:5396002/1‑150 (MQ=255)
                                                                                                                      ttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCt                               >  1:8548879/1‑150 (MQ=255)
                                                                                                                        cAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaa                             <  1:169050/150‑1 (MQ=255)
                                                                                                                         aGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaac                            >  1:8722886/1‑150 (MQ=255)
                                                                                                                           ttggtgTTTTTGGTCGGCGGGAATTTTAATAGCGTCGGAGAGGTAATTTTTGGCGGTTTCGGCGTCGGTGAGCGCCAGTTGCCACTCATCGCGCGCCGGGTAAAGACGAATGTTATAGGTTTTGCCTGCTTGCGAGGAGCGATCTaacaa                          >  2:332386/6‑150 (MQ=255)
                                                                                                                           ttGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaa                          <  1:4531807/150‑1 (MQ=255)
                                                                                                                           ttGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaa                          >  2:1034348/1‑150 (MQ=255)
                                                                                                                             gCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCCGGAGCGATCTAacaaca                        >  2:7138240/1‑150 (MQ=255)
                                                                                                                             gCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAacaaca                        <  1:5410077/150‑1 (MQ=255)
                                                                                                                             gCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAacaaca                        <  2:2704874/150‑1 (MQ=255)
                                                                                                                                 tttttGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTc                    <  1:66797/150‑1 (MQ=255)
                                                                                                                                 gttttGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTc                    <  1:5267654/149‑1 (MQ=255)
                                                                                                                                   tttGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCt                  >  2:2140214/1‑150 (MQ=255)
                                                                                                                                     tGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTgc                <  2:9140871/150‑1 (MQ=255)
                                                                                                                                        tCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCa             >  1:4465363/1‑150 (MQ=255)
                                                                                                                                           ccgccAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGt          <  2:2458687/150‑1 (MQ=255)
                                                                                                                                            cgccAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGtt         <  1:8343751/150‑1 (MQ=255)
                                                                                                                                                aaaTTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGTTTTTGCCTGCTTGCCAGGAGCGATCTATCAACACTTCCTGCGCACGTTCaat     >  2:1855549/1‑149 (MQ=255)
                                                                                                                                                aaaTTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaaa     <  1:5696332/150‑1 (MQ=255)
                                                                                                                                                 aaTTTAAATGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaaaa    <  2:7914487/150‑1 (MQ=255)
                                                                                                                                                 aaTTTAAAAGCGTCGGAGAGGTTATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGTTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaaaa    <  1:4702963/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGT  >  NC_000913/4244479‑4244775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: