Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,097,132 T→G 6.2% intergenic (+303/+433) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,097,1320TG 6.2% 908.9 / 10.2 346intergenic (+303/+433)ycdU/serXputative inner membrane protein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (89/228);  new base G (6/23);  total (95/251)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 361 total aligned reads displayed.

AAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGG  >  NC_000913/1096984‑1097281
                                                                                                                                                    |                                                                                                                                                     
aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATCTGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCtt                                                                                                                                                      <  2:8384925/150‑1 (MQ=17)
aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATCGGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCtt                                                                                                                                                      <  1:6367354/150‑1 (MQ=17)
aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCtt                                                                                                                                                      <  2:7690650/150‑1 (MQ=32)
 aaaaGCCCGGCCTTTCGTACGAGCTCTTCTTTAAATCGGGGGGTGCGGGGGGGATTGCCTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCAGGGTCTTTCCACCTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTtc                                                                                                                                                     <  1:1928984/150‑1 (MQ=11)
      cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATc                                                                                                                                                <  2:1225157/150‑1 (MQ=32)
          tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATccccc                                                                                                                                            <  1:8594194/150‑1 (MQ=32)
          tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATccccc                                                                                                                                            <  2:7507024/150‑1 (MQ=32)
           aCTTTCGTACGAGCTCTTCTTTCACTATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCGCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCAGcccccc                                                                                                                                           <  2:4502481/150‑1 (MQ=255)
                  tACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCCCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGgtgtgt                                                                                                                                    <  1:641787/150‑1 (MQ=17)
                  tACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGgtgtgt                                                                                                                                    <  2:8234401/150‑1 (MQ=32)
                  tACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGgtgtgt                                                                                                                                    <  2:212772/150‑1 (MQ=32)
                  gccGAGCTCTTCGGTAAAGCGGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCCCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGgtgtgt                                                                                                                                    <  2:2762964/148‑1 (MQ=11)
                   aCGAGCTCGTCTTTAAAGCGGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGCCCCCCGGTCGGGGCTTCTCATCCCCCCGGtgtgtg                                                                                                                                   <  2:8642264/150‑1 (MQ=11)
                      aGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCaa                                                                                                                                <  2:5758638/150‑1 (MQ=32)
                      aGCTCTTCTTGAAATAGGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCaa                                                                                                                                <  2:8133407/150‑1 (MQ=17)
                          cttcttTACATAGGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACGGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATata                                                                                                                            <  2:798819/150‑3 (MQ=2)
                          cttcttTAAATATGGCGGTGTGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGTCAGCCCGCTCTCTGCGTTCACGGTGTGTCCTACTGGGTTTCGCAAGTTTTCGACCCACTGTCGGGGCTTCTCTTCCCCCCGGTGTGTGCAATaaa                                                                                                                            <  2:3037915/150‑3 (MQ=11)
                           ttcttTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATacac                                                                                                                           <  2:6487419/150‑1 (MQ=34)
                           ttcttTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATacac                                                                                                                           <  1:1593968/150‑1 (MQ=34)
                              tttAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCAGGtgtgtg                                                                                                                                   <  2:8485926/139‑1 (MQ=17)
                               ttAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGATCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGaaa                                                                                                                       <  2:3867011/150‑1 (MQ=17)
                                tAAATAGGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGaaaa                                                                                                                      <  1:4383675/150‑1 (MQ=21)
                                  aaTATGGCGGTGAGGGGGGGATTGACTAGCTTCGCTCGCCCTGCGGGCAGCCCGCACAAAGAGATAACGCTCTGTCCAAATGGCTGTCGCCACTTGTCGACCCCCGGTCGGGGCTACTCATCACCCCCGTGGGAGCAAAAAACGaaaaaa                                                                                                                    >  1:5288616/1‑150 (MQ=11)
                                    tatGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGaaaaaaaa                                                                                                                  <  2:10008212/150‑1 (MQ=34)
                                    tatGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGGCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGaaaaaaaa                                                                                                                  <  1:4865470/150‑1 (MQ=21)
                                      tGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGc                                                                                                                <  1:6501416/150‑1 (MQ=34)
                                      gggCGGTGTGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCAGGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGc                                                                                                                <  1:4941798/149‑1 (MQ=21)
                                        gCGGTGCGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGccc                                                                                                              <  1:1005709/150‑1 (MQ=21)
                                         cGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCg                                                                                                             <  2:480824/150‑1 (MQ=34)
                                               ggggggtTTTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGCCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACttt                                                                                                       <  1:3014518/150‑1 (MQ=255)
                                                ggggggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTc                                                                                                      <  2:8816135/150‑1 (MQ=34)
                                                   gggATTGACTCGCTTCGCGCGCCCGGCGGGCAGCCCGCTCCCTGCGTTCACGGTCTGTCCACCTGGCTGTCGCCAGTTGTCGCCCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTa                                                                                                   <  1:7821128/150‑1 (MQ=11)
                                                    ggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTAc                                                                                                  <  1:4252117/150‑1 (MQ=34)
                                                      aTTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGa                                                                                                <  2:139956/150‑1 (MQ=34)
                                                       ttGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACAAGAAAAAAAAGCCCGTACTTTCGTACGAg                                                                                               <  1:9747786/150‑1 (MQ=21)
                                                          aCTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCtt                                                                                                                                                      >  2:1483713/1‑92 (MQ=33)
                                                           cTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGctct                                                                                           <  1:8364362/150‑1 (MQ=34)
                                                                   gctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTaaa                                                                                   <  1:9847352/150‑1 (MQ=34)
                                                                        cccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATgg                                                                              <  1:2098100/150‑1 (MQ=34)
                                                                        cccTGCGGGCAGCCCGCTCACTGCGTGCACGGTCTGTCCAACTGGCTGTCGCCAGTTGCCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATgg                                                                              <  1:3482257/150‑1 (MQ=21)
                                                                         ccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTc                                                                                                      <  1:1402491/125‑1 (MQ=35)
                                                                             cGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTg                                                                         >  1:6718476/1‑150 (MQ=34)
                                                                                    cccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggg                                                                  >  2:3761160/1‑150 (MQ=34)
                                                                                    cccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggg                                                                  >  1:7160210/1‑150 (MQ=34)
                                                                                      gtctgaCTGCGTTCAATGGATGTCGTACTGGTTGTCGCGAGTTGTCGACCCCCGGTCGGGGCTTCTGTTCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGa                                                                <  2:3452774/145‑1 (MQ=11)
                                                                                      cGCTCACTGCGTTCACGGTCTTTCCATCTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGTTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGa                                                                <  1:2813642/150‑1 (MQ=255)
                                                                                      cGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCg                                                                            >  1:7485476/1‑138 (MQ=34)
                                                                                       gCTCACTTCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTTTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAt                                                               <  1:2356002/150‑1 (MQ=21)
                                                                                       gCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAt                                                               <  1:3261666/150‑1 (MQ=34)
                                                                                          cacgtcGTTCTCGGTCTTTCCAACTTGCTGTCGCCAGTTGTCGACCCCCTGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                            <  2:553733/145‑1 (MQ=11)
                                                                                            cTGCGTTCCCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACt                                                          <  2:4016704/150‑1 (MQ=21)
                                                                                            cTGCGTACACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACt                                                          <  1:9419126/150‑1 (MQ=21)
                                                                                               cGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgc                                                       >  2:1735047/1‑150 (MQ=34)
                                                                                                 ttCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATata                                                                                                                            >  2:7796148/1‑77 (MQ=14)
                                                                                                  tCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttc                                                    <  1:1951111/150‑1 (MQ=34)
                                                                                                   cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATAGACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttcg                                                   <  1:2576790/150‑1 (MQ=17)
                                                                                                    aCGGCCGGTCCAACAGGCTGTCGACTGTTCTCCCCCCCCGGTCGGGGGTTCTCCTCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttcgc                                                  <  1:1509005/150‑1 (MQ=11)
                                                                                                       gTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTtcgctcg                                               <  2:5490631/150‑1 (MQ=34)
                                                                                                        ccTGCCCAACTGGCGGCCGCCCGCCGGCGCCCCCCCCTCGGGGCGTCTCCGGCCCCCGGTGTGTGCAATACACGAAAATAAAGCCCGTACTTTCGTTAGAGCTCTTCTTTAAATATGTCGGTGAGGGGGGGATTGACTCGCTtcgctcgc                                              <  2:2248443/149‑1 (MQ=11)
                                                                                                          tGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGctct                                                                                           <  2:9286522/103‑1 (MQ=35)
                                                                                                           gTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGgtgt                                                                                                                                      >  1:8663569/1‑59 (MQ=35)
                                                                                                            tCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTaaa                                                                                   >  1:6047477/1‑109 (MQ=35)
                                                                                                             ccAACTGGCTGTCGCCTGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATAAACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGc                                         <  1:4605203/150‑1 (MQ=17)
                                                                                                             ccAACTGGCGGTCGCCAGCCGTCGACCCCCGGTCTGGGCGTCTCCGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGc                                         <  1:8212458/150‑1 (MQ=11)
                                                                                                              ctagatgCTGTCGCCAGGTGACGACCCCCTGTCGGGGCGTCTCTGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCg                                        <  1:6195514/144‑1 (MQ=11)
                                                                                                               aaCTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGGCTCGCTTCGCTCGCCCTGCgg                                       >  1:9152551/1‑150 (MQ=21)
                                                                                                                agtGGCTGTCGCCAGGTGTCGCCCCCCGGTGGGGGCTTCTGCGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCggg                                      <  2:591076/148‑1 (MQ=11)
                                                                                                                aCTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCggg                                      <  2:7437363/150‑1 (MQ=34)
                                                                                                                  tGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCg                                                                                                     <  1:5628664/85‑1 (MQ=38)
                                                                                                                  tGGCTGTCCCCAGTTGTCGACCCCCGGACGGGGCGTCGCAGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCa                                    <  2:9035598/150‑1 (MQ=11)
                                                                                                                   ggcgacagCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTAAGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCGGCGGGCAg                                   >  2:6171811/8‑150 (MQ=255)
                                                                                                                   ggCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCCTCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAg                                   <  1:375161/150‑1 (MQ=21)
                                                                                                                    gCTGTCGCCATTCGTCGTCCCCCTGTCTGGGCGTCTCCGCCCCCCGGTGTGTGCAATAGACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGc                                  <  2:4891813/150‑1 (MQ=11)
                                                                                                                      tGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggg                                                                  >  2:3978709/1‑116 (MQ=35)
                                                                                                                      tGTCGCCAGTTGTCGACCCCCGGTCGGGGCGTCTCATCCCCCCGGTGTGTGCAAAACACGAAAAAAAACCCCCTACATAAGAACGAGCTCGTCTTTAAATATGGCGGGTAGGGGGGGGTTTTCACGCAACGCTCGCCCTGCGGGCAGccc                                >  1:4112807/1‑150 (MQ=11)
                                                                                                                       gacGCCAGTCGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCg                               <  1:5739273/148‑1 (MQ=21)
                                                                                                                           ccAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCa                           >  1:542079/1‑150 (MQ=34)
                                                                                                                                tGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCg                      <  1:3652776/150‑1 (MQ=34)
                                                                                                                                 gTCGACCCCCGGGCGGGGCGTCACCTCCCCCCGGCGTGAGCAAGCCCCGAAAAAATAGCCCGTACTTGCGAACGTTCACCTCTTGAACTCCGGCGGTGAGGGGGGGAGTGCCTCGCTTCGCGCTCACTGCGGGCAGCCCGCTCACTGCGt                     <  2:7399587/150‑1 (MQ=2)
                                                                                                                                  tCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGtt                    >  1:7927250/1‑150 (MQ=34)
                                                                                                                                  tCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGtt                    <  1:3871415/150‑1 (MQ=34)
                                                                                                                                  tCGACCCCCGGTCGGGGCGTCTCAGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGAACTTTCGGACGAGCTCTTCTTTACAGAGGGCGGGGAGGGGGGGCGGGACGCGCGTCGCGCGCCCGGCGGGCAGCCCGCGCCCTGCggg                    >  2:5692835/1‑148 (MQ=255)
                                                                                                                                    gACCCCCTGTCGGGGCTTCGCCTCCACCCGGTGTGTGCAAAACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCa                  <  2:7105235/150‑1 (MQ=11)
                                                                                                                                    gACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                            <  2:956641/108‑1 (MQ=35)
                                                                                                                                    gACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                            >  1:956641/1‑108 (MQ=35)
                                                                                                                                    gACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCa                  >  2:5197619/1‑150 (MQ=34)
                                                                                                                                       ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGa                                                                >  2:4215536/1‑101 (MQ=32)
                                                                                                                                       ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACgg               >  2:9357920/1‑150 (MQ=32)
                                                                                                                                        ccccctCGCGGCTCCTCATCCCCCCGGTGTGTGCAATACACCAAAAAAAACCCCGAACTTTCGTACGACCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGt              <  1:1710708/145‑1 (MQ=11)
                                                                                                                                        cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttcgc                                                  >  2:6886520/1‑114 (MQ=32)
                                                                                                                                        cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttcgc                                                  <  1:6886520/114‑1 (MQ=32)
                                                                                                                                         ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTc             >  1:6145420/1‑150 (MQ=32)
                                                                                                                                          cGGTCTGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCt            <  2:3228506/150‑1 (MQ=17)
                                                                                                                                             tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc         <  2:6323697/150‑1 (MQ=32)
                                                                                                                                               ggggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTtcgctcg                                               <  1:3009541/110‑1 (MQ=32)
                                                                                                                                               ggggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCa       >  2:1902915/1‑150 (MQ=32)
                                                                                                                                                  gCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACt    >  1:2872775/1‑150 (MQ=32)
                                                                                                                                                    ttCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTa                                                                                     >  2:6752040/1‑67 (MQ=35)
                                                                                                                                                    ttCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGTGCGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGAGTTCACGGTCTGTCCAACTgg  >  2:1577239/1‑150 (MQ=12)
                                                                                                                                                    ttCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc         >  2:2599076/1‑143 (MQ=32)
                                                                                                                                                    |                                                                                                                                                     
AAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGG  >  NC_000913/1096984‑1097281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: