Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,595 | T→G | 11.6% | intergenic (+61/‑135) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,595 | 0 | T | G | 11.6% | 606.7 / 23.6 | 217 | intergenic (+61/‑135) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base T (21/166); new base G (27/2); total (48/169) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.00e-19 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 240 total aligned reads displayed.
GAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGAC > NC_000913/164448‑164744 | gaTGAATGGGCGTTATCCCAAACATGTCTGGCCGGCCGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTGGTATGGTTGGTTTT‑CTCCCTCGCCCTGGGGGTGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcacc < 2:9591041/150‑1 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccc > 2:8380001/1‑150 (MQ=255) aacgggCTTTATCCCAAACTTGTCGGGCCGCACGACCGGTAAATTACTGCACCGCGGCTAGTTATTAAAATTTTTTGGTATGGTTGTTTTT‑CTCCCGCTCCGGGGGGGAGTGGGCGGGGGTTTGGGCCTCAGCGCGCACGTtcaccctca < 2:7901307/147‑1 (MQ=255) aaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGAGGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcac < 2:7373564/150‑1 (MQ=255) aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTgcccccccccc > 2:5954314/1‑145 (MQ=255) aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGGCCGGtcaacctcacc > 1:200063/1‑150 (MQ=255) gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCTTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcaccc < 2:6434925/150‑1 (MQ=255) ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGgcacccacaccca > 1:8204305/1‑149 (MQ=255) ggTTATCCAAAACATGTGTGGCCGGACGCCCCGGCAAATACTGCCCCTACGCGACGGACTAAAATGTTTTCTTATGGTTGTTTTT‑CTCCCTCTCCCTGTGGTTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTaa < 2:6929517/149‑1 (MQ=25) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTGGCCCacacccacacccaca > 1:9688402/1‑147 (MQ=38) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAAcccaca > 1:7561783/1‑147 (MQ=255) ccAAACATGTCTGGCCGTACGACCCGGCAAATACTGCACCTAAGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑ATCCCTCTCCCTGTGGGAGAGGGCCGCGGTGAGGGCATCAGCGCGCACGTTCACCAACACCCCAAcacacta > 1:7883847/1‑144 (MQ=37) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCACAcccacac > 2:9507566/1‑146 (MQ=38) cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCCCCCCCGAACCCCCTcc > 2:9015910/1‑150 (MQ=38) aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCGCCCACACCCAAAACCACAccc > 2:5443308/1‑150 (MQ=37) agggcTGGCCGGACGCCCCGGAAAATCCGGCACCGACGCGAGGGCCTAAAAATTTTTCGTAAGGTTGGTTTT‑CCCCCACTCCGGGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 1:9029265/146‑1 (MQ=255) aTGTCTGGCCGTACCCCCCGTAAAATACTCCACCGACGCGACGTACTAAAATTTTTTCGTATGGTTGTTTTC‑CTCCATCTCCCTGGGGGTGTGGGCGGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 1:6548703/150‑1 (MQ=255) aTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACTCCCACaa > 1:9155738/1‑150 (MQ=255) tgtgtgGCCGGACGACCCGCAAATTCCTCCACCGACGCCAGTCACTAAAAATTATACGTAAGTTTGTTTTT‑CACCCTCACCCTTGGGTTGTGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:346953/150‑1 (MQ=255) tGTCGGGCGGGACGACCCGGCAAATACGGCCCCGACGCGCCGGACTAAAAATTTTTCTTATGGTTGTTTTT‑CCCCCACACCCTGTGGTAGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:2073364/150‑1 (MQ=255) cTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGAGCCCGCACCCCAACCCACTCCCACAAcggg > 1:1206537/1‑149 (MQ=37) gcccGGACGACCCGCCAAACACGGCCCCGCCGCGCCGGACGAAAAAGTATGCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGgaga < 2:5261615/148‑1 (MQ=37) gcccGCACCACCCCGCAAATACAGCACCGACCCGACGCACCAAAAACTATTCGTAAGGTTGTTTTA‑CACCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga < 2:9878060/148‑1 (MQ=37) gCCGGACGCCCCGGAAAAACCGGCCCCGCCGCGACGGACGAAAATTTTTTCGTACGGTTGTTTTA‑CCCCCTCCCCCGGGGGGAGCGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag < 2:3803005/150‑1 (MQ=255) gCCGGACGACCCGGCAAATACCGCCCCGACGCGAGGGCCGAAAAAGTATTCGGATGGTTGGTTTT‑CGCCCTCCCCGGGGGGTAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:8745086/150‑1 (MQ=255) ccgcccgacCCGCAAAATACTGCACCGACGCGACGGACCAAAAATTTTTCGTATGGTTGTTTTT‑CCCCCGCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 1:4182843/145‑1 (MQ=37) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTAACCCCCACCCAAACCCACACACGCAAGGGAGAggg > 2:3243707/1‑150 (MQ=37) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCAAAACCGCACCCACAAGGGGGAggg > 2:2452351/1‑150 (MQ=37) cGGACCACCCGCAAAAAACTCCACCGACGCCACGCAATAAAAATTATTC‑TAATGTTTGTTTTACACCCTCTCCCTGTGGTTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAggg < 2:7938728/150‑1 (MQ=255) cgccccGGCAAATACGGCACCGCCGCGCCGGACGAAAAAGTATTCGTACGGTGGGTTTT‑CTCCCTCGCCCTGGGGGAGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc < 2:1620714/147‑1 (MQ=255) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCCCCCAAACCCCCACCCACCAAGGGGAGGGGGCCgcgc > 1:4725496/1‑147 (MQ=25) ccGGCAAACACTCCACCGACGCGACGCACGAAAAAGTATTCTTACGTTGGGTTTT‑CTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 2:2893290/150‑1 (MQ=37) gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:6547352/150‑1 (MQ=255) aTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCCCACCCAAACCCACACCCTCAAGGGAGAGGGGGCCCAGCGAGcacc > 1:8375064/1‑149 (MQ=37) gccccGACGCGCGTGACGAAAAATTATGCGTAAGGTTGTTTTT‑CTCCCTCTCCGTGGGGGAGCGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAttt < 1:4038195/147‑1 (MQ=255) cACCGACGCGCCGGACTAAAAATTTTTCTTATGTTTGTTTTT‑CTCCCTCTCCGTGTGGTAGTGGGGCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:7215339/150‑1 (MQ=255) cccgccgGGACGGACTAAAATGTATTCGTACGGTTGGTTTT‑CTCCCTCTCCCTGGGGGTGCGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg < 1:7339851/149‑1 (MQ=255) aCCGACGCGAGGGACTAAAATGTTTTCGTATGGTTGGTTTT‑CTCCCTCTCCCTGTGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg < 2:202093/150‑1 (MQ=38) ccgcggcgccgGACGAAAAATTATTCGTATGGTGGGTTTT‑CCCCCGCCCCGTGTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:8896777/145‑1 (MQ=255) ccgccgCGTCGGACGAAAAATTATTCGTATGGTTGTTTTC‑CTCCCACTCCGTGTGGGAGAGGGCCGGGGTTCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:665824/150‑1 (MQ=255) ccgccgCGACGTATTAAAATGTATTCTTCTGGTTGGTTTT‑CCCCCTCGCCGGGTGGGTTTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:1087648/150‑1 (MQ=255) ccGACGCGCCGGACTAAAATTTTTTCTTAAGGTTGGTTTT‑CTCCCACTCCCTGTGGTAGCGGGCCGGGTTGAGGGAATCAGCGCGCACGTTCACCCTCACCATAACCCTCTCCCACAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:6988797/150‑1 (MQ=255) ccGACGCGACGGACTAAAAAGTATTCGTATGGTTTTTTTT‑CCCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:6184702/150‑1 (MQ=38) ccGACGCCACGTACCAAAACGTTTTCTTACGGTTGTCTTT‑CTCCCTCCCCCTGTGGGAGTGGGCGGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:4295399/150‑1 (MQ=255) gacgcgccgGCCTAAAAATTATTCGTATGGTTGGTTTG‑CGCCCTCTCCCTGTGGGCGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 1:8473619/150‑1 (MQ=255) gagggacgAAAAAGTTTTCGTATGTTTGGTTTA‑CTCCCTCTCCCTGTGGTAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGGCCGATCGAGCACAAATTTTGAGAGatat < 1:4880652/147‑1 (MQ=255) caattATCCGTACGGTTGGTTTC‑CTCCCTCGCCCTGTGGGAGCGGGCCGGGGTGCGGTCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt < 2:1293290/146‑1 (MQ=255) aaaGTAGTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt < 1:8969281/150‑1 (MQ=255) atgttttCGTATGGTTGGTTTT‑CTCCCTCTCCCTGGGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:1568133/145‑1 (MQ=255) ttATTGGTATGGTTGTTTTT‑CTCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:5544567/149‑1 (MQ=39) gTATTCTCACGGTGGGTGTC‑CTCCCGCGCCCGGGGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:5219806/150‑1 (MQ=255) tCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 2:7407727/150‑1 (MQ=255) gTACGGTTGGTTTT‑CTCCCTCGCCGTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 1:5020531/150‑1 (MQ=255) tAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCCCCCCCACCCAAACCCACGCCCACCAGGGCGAGGGGGCCGATCGCGCCCAAAAGTTGGGTGGTATCTTCTTCTGTCTTgggaccg > 2:2477422/1‑144 (MQ=16) aaGGTTGGTTTC‑CGCCCTCTCCCTGTGGGAGAGGGCCGGGGTTCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 2:731860/150‑1 (MQ=255) tggttggttTT‑CTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:9934610/149‑1 (MQ=255) tggttggttTT‑CCCCCTCCCCGGGTGGTAGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 2:7610678/149‑1 (MQ=255) tgttttt‑CGCCCACCCCGTGTGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:504938/147‑1 (MQ=255) ggttTT‑CGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 1:5699035/150‑1 (MQ=255) ggttTC‑CTCCCCCGCCGGGGGGGTGTGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 1:532533/150‑1 (MQ=255) gttTG‑CTCCCTCGGCGGGGGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg < 1:8687361/150‑1 (MQ=255) ttt‑CTCCCGCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaa < 1:8870519/150‑1 (MQ=255) tccctcgccctGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:3328099/150‑1 (MQ=255) catctccggGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:3996844/148‑1 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTAACCCAAACCCTCACCCGCCAGGGGGAGGGGACCGAACGTGCCCAAACTTTGAGTGATATATTCTTATTTAGTGTTAAACAAGAAACGCAAATCggg > 2:6042933/1‑150 (MQ=16) tgtgGGTGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc < 1:4051376/150‑1 (MQ=255) ggtgGGTGAGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc < 1:9742327/149‑1 (MQ=255) ggtgGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc < 2:7424935/149‑1 (MQ=255) ggtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc < 2:723858/149‑1 (MQ=255) gggcgcgggCCGGGGGGCGGGCCTCAGCGCGCCCGGTCACCCTCACCCGACCCCTCTCCCTCACGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATCTCTTCGTCTGTCTTGTAACAGAAGAACAGAACATCGGGCTTTTGCGcc < 2:5676684/144‑1 (MQ=255) gggAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCACCCCCACCCCAACCCACACCCTCAAGGGAGAGGGGCCCCAACCAGCCCAAAATTTGAGCGGTATATTCTTAGGTGTTGTGACCGAAGAACAGCAAAAAGGGCGGTTGGGcc > 2:4110753/1‑150 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTg < 2:8309527/150‑1 (MQ=255) agagGGCCGGGGGGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTGCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGa < 1:9488717/150‑1 (MQ=255) gtgggCGGGGGTGCGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGaa < 1:3604377/148‑1 (MQ=255) gggCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata < 2:6185699/150‑1 (MQ=255) ggCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAatat < 1:4400475/150‑1 (MQ=255) gCCGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAtt < 2:3412935/150‑1 (MQ=255) cGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 2:5044886/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:2452351/150‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:1184919/150‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:6302630/150‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:9155588/150‑1 (MQ=255) gTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGgag < 2:1844673/150‑1 (MQ=255) ggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:3239988/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:4238710/150‑1 (MQ=255) gccATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCACGTGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:2936518/148‑1 (MQ=255) cctCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGACTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc < 1:4047671/148‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc < 2:4684237/150‑1 (MQ=255) ctCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa < 2:804815/149‑1 (MQ=255) tCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt < 1:6606622/150‑1 (MQ=255) cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATg < 2:7128917/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 1:4932462/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 1:4252161/150‑1 (MQ=255) gcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGCTCGAGCACAAATTTTGAGAGATATCTTCGTCTGTCTTGTAACCGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCggg < 1:7681242/150‑1 (MQ=255) gcACGGGCACCCTCCCCCAAACCCAATCCCTCAAGGGAGGGGGGACCGCTGGAGCACAAAATTTGAGCGATATATTCATATGTCTTGTAACAGAAGAACAGAAAAACGGGCTTTTGAGCCAGAAGATTTCGGAGAAAAAGGAGGGCCggg > 2:2351667/1‑150 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGGGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGaa > 2:4026196/1‑150 (MQ=255) aCGTACACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGaa < 1:6939409/150‑1 (MQ=255) aCGATCACCCACACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCATTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGaa < 2:190292/150‑1 (MQ=255) cGTTCACCCTCACCCGAACCCTCTCCCTCACGGGAGAGGGGACCGCTCGAGCACCAATTTTGAGAGCTATCTTCTTCTGTCTCGTAACAGAAGTACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAAt < 2:5513558/150‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGAc < 1:726669/150‑1 (MQ=255) | GAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGAC > NC_000913/164448‑164744 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |