Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 5.4% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 5.4% 794.7 / 22.4 288noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (147/115);  new base A (8/7);  total (162/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287001‑1287298
                                                                                                                                                    |                                                                                                                                                     
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCGTCGCCCTTCGGGTCGTTGCCTGCGGCCCCGCGCTCTCCCAGGCGCGCGa                                                                                                                                                      >  1:4449607/1‑150 (MQ=14)
   cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                   >  1:9979703/1‑150 (MQ=255)
    ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTTCGAGCTTCGCCCTTCGGGTCGATTCCTGAGGCATAGCGCTCTCGCGGCCGCTAGAGTCg                                                                                                                                                  >  2:8995329/1‑150 (MQ=14)
    ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACCCTCTCTCGCGGGCGCTCGAGTCg                                                                                                                                                  >  1:959769/1‑150 (MQ=255)
     cGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGa                                                                                                                                                 <  1:2346347/150‑1 (MQ=255)
     cGCATGGGCAGAATATTGGATTGCGGTTTCGCTTGTGATGTCAGGTCGTTTTGAATGTGCTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGa                                                                                                                                                 <  1:69696/150‑1 (MQ=14)
           ggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTg                                                                                                                                           >  1:7131140/1‑150 (MQ=255)
            gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTgg                                                                                                                                          >  1:5817201/1‑150 (MQ=255)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCTCATCGCCCTTCGGGGCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGACTCGAACCGTGGTc                                                                                                                                        >  1:4993627/1‑150 (MQ=14)
                        aTTGCGGATTCGCTTGAGAGTTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtctc                                                                                                                              <  2:6807311/150‑1 (MQ=255)
                         ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCCGCCACCCACACACGCTGGCGCGCGAGTCGAACCATGGTCGAAGCTTCTCa                                                                                                                             >  1:8827299/1‑150 (MQ=14)
                          tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAAAGCTCTCACGCGGGCGCTCGAGTCGAAACTTGGTCGAAGCTTCTCaa                                                                                                                            >  2:9331001/1‑149 (MQ=14)
                            cGGATTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATcc                                                                                                                          <  2:7172643/150‑1 (MQ=255)
                            cGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGATCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAAcc                                                                                                                          >  2:7320903/1‑150 (MQ=255)
                            cGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATcc                                                                                                                          >  2:7281292/1‑150 (MQ=255)
                                ttCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTcc                                                                                                                      <  2:925721/150‑1 (MQ=255)
                                ttCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTTGCGCTCGAGTCGAACCTGGGTCGAAGCTTATCATCCTTcc                                                                                                                      >  2:2358124/1‑150 (MQ=14)
                                    cTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGAATCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                                  >  2:7865933/1‑150 (MQ=255)
                                     ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTAGAAGCTTCTCAACCATCCCCCCa                                                                                                                 >  1:713913/1‑150 (MQ=255)
                                     ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCTTGCGCACGAGTCGAACCATTGTCGAAGCTTCTCAACCTTCCCCCCa                                                                                                                 >  1:8363896/1‑150 (MQ=14)
                                     ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTAACGCAGGCCCAACCGTCCAAACGTGGTCGAAGCTTATCATACATACCCGcc                                                                                                                 >  1:2304287/1‑149 (MQ=14)
                                     ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCACTCGCTGGCGCTCGAGTCGAACCATGGTCGAAGCTTCTCATACTTccccccc                                                                                                                 >  1:6387780/1‑147 (MQ=14)
                                      tGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                                <  1:5355468/150‑1 (MQ=255)
                                             tCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCATCCTTCCCCGCCTGGGCAGa                                                                                                         >  2:8087813/1‑150 (MQ=255)
                                               aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCAACGGTCTCTCGCTTTCGCTCGAGTCGAACCTTAGTCGAAGCTTCTCAAACTTCCCCGAATggttcggaa                                                                                                       >  2:196369/1‑143 (MQ=11)
                                                  gACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGACGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                                                    >  2:3260115/1‑150 (MQ=38)
                                                       ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                            >  2:4831120/1‑137 (MQ=37)
                                                            aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACTCGCTTGCGCACGAATCGAACCTTGGTAGAAGCTTCACATCCTTCCCCCCATGGGCAGAATATTTAATTTCggt                                                                                          >  2:3566754/1‑149 (MQ=14)
                                                                  ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                    <  2:8902823/150‑1 (MQ=37)
                                                                   gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATCTAATTGCGGATTCGTTg                                                                                   <  1:140258/150‑1 (MQ=38)
                                                                     ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCATCCTTCCCCGCCTGGGCAGAATATGTAAGTGCGGATTCGGTggg                                                                                 >  2:2080657/1‑150 (MQ=11)
                                                                      gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                                >  2:472195/1‑150 (MQ=255)
                                                                        gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                              >  2:5059538/1‑150 (MQ=255)
                                                                         ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCGCGCTGGCGCTCCAGGCGAACCGTCGTCGAAGCATCTCATCCTTCCCCGCCTGGGCCGAAGAATTAATTGCGGAGTCGGTGGGGAGt                                                                             >  2:8664879/1‑150 (MQ=11)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTc                                                                           >  2:9939859/1‑150 (MQ=255)
                                                                            gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAg                                                                                                                                    <  1:1586070/92‑1 (MQ=33)
                                                                            gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTAGAACCGTGGTCCGAGCTTGTCGTACATACCCGCAGGGGCAGAGTATATAAATGGGGTGTCGGTGGGaattaca                                                                          >  2:1423244/1‑145 (MQ=11)
                                                                                  ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                    <  1:6073103/150‑1 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                                >  2:5518233/1‑150 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                                >  2:5900725/1‑150 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCAACCGTCCCCGCATGGGCAGAAAATTTAATATCGGATTCGGTGGGCCGGTCAgggggggttg                                                                >  2:2805345/1‑143 (MQ=14)
                                                                                        ttcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                              <  1:7769750/148‑1 (MQ=255)
                                                                                         gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                             <  1:5209586/150‑1 (MQ=255)
                                                                                          cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGCACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                            >  2:6194382/1‑150 (MQ=255)
                                                                                          cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAAAATTTAATATCGCAGGCGTGGGGAACGTCAGGGACGTTTGATAg                                                            >  2:839356/1‑150 (MQ=11)
                                                                                             tGCGCGCTTCGCCGTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         <  1:436165/150‑1 (MQ=255)
                                                                                              gcgcgcTTCGCCGTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAt                                                        <  1:7841141/150‑1 (MQ=255)
                                                                                               cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGa                                                                                          <  1:9084469/115‑1 (MQ=39)
                                                                                                    ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGt                                                                                                                                                    >  2:7037303/1‑52 (MQ=35)
                                                                                                     tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCg                                                                                                                                                       <  1:2699625/48‑1 (MQ=32)
                                                                                                     tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCg                                                                                                                                                       >  2:2699625/1‑48 (MQ=32)
                                                                                                     tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                                 <  1:944766/150‑1 (MQ=255)
                                                                                                       gCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTCATGGTGGTggg                                               <  1:8042082/150‑1 (MQ=255)
                                                                                                           ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  1:3287414/150‑1 (MQ=255)
                                                                                                           ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  1:653181/150‑1 (MQ=255)
                                                                                                            tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCCTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                          <  2:760864/150‑1 (MQ=255)
                                                                                                               ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                       >  2:8981803/1‑150 (MQ=255)
                                                                                                                gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                      <  1:7409344/150‑1 (MQ=255)
                                                                                                                   gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGa                                   <  2:10023593/150‑1 (MQ=255)
                                                                                                                   gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGa                                   <  2:4079078/150‑1 (MQ=255)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                  <  2:8827299/150‑1 (MQ=255)
                                                                                                                     ggCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAGTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                 <  1:8664879/149‑1 (MQ=255)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCt                               >  2:7965737/1‑150 (MQ=255)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                      >  1:9674230/1‑143 (MQ=255)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                      <  2:9674230/143‑1 (MQ=255)
                                                                                                                        cttcGGCAACGCCCTCTCGCTGGCGCTCGAGTCGATCCTAGGTCGATGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                              <  2:3914741/147‑1 (MQ=17)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTgg                                                                                                                                          <  2:399160/41‑1 (MQ=38)
                                                                                                                         tGCGGCAACGCTCTCCCGCTGGCGCTCGAGACGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATATAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGCAGGATTCGAACCTTc                             <  1:6667410/150‑1 (MQ=17)
                                                                                                                          gCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggt                                                     <  1:1000211/125‑1 (MQ=255)
                                                                                                                          gCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg                            <  2:5396759/150‑1 (MQ=255)
                                                                                                                             gCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAg                         >  2:8627520/1‑150 (MQ=255)
                                                                                                                              cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAatat                                                                                                     <  1:1796461/73‑1 (MQ=39)
                                                                                                                                aCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                      >  2:5973465/1‑150 (MQ=255)
                                                                                                                                aCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATCTTAAATTGCGCAGGCGATGGGACCGTCAGGGACGTTTGAAAGTGATGGGGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                      >  2:2284488/1‑150 (MQ=18)
                                                                                                                                 cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCATACCCGCATGGGCAGAATATTTAATTGCGGCGTCGTTGGGAAGTTACGGGACTTTGTTAAGGGATGGTGGGGGGGGAAGGAGTCGAACCTTCGCAGTCGa                     >  1:9895282/1‑150 (MQ=17)
                                                                                                                                  gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                    >  2:9467502/1‑150 (MQ=255)
                                                                                                                                  gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                    <  1:3395886/150‑1 (MQ=255)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg                            >  2:9797914/1‑142 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtt                                                                   <  1:7043855/101‑1 (MQ=255)
                                                                                                                                    tctctcGCTGACGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCCTAACTTCGAAGTCGATGa                  >  2:7117041/1‑150 (MQ=17)
                                                                                                                                     ctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                                <  2:442418/103‑1 (MQ=255)
                                                                                                                                      tctcGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg                <  1:8479163/150‑1 (MQ=255)
                                                                                                                                      tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg                >  2:5266167/1‑150 (MQ=255)
                                                                                                                                      tctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  <  1:623612/148‑1 (MQ=255)
                                                                                                                                       ctcGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg               <  1:4621267/150‑1 (MQ=255)
                                                                                                                                       ctcGCTGGCGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg               <  2:6421367/150‑1 (MQ=255)
                                                                                                                                       ctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAgg                                                                        >  1:3074832/1‑93 (MQ=255)
                                                                                                                                        tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc              >  2:562731/1‑150 (MQ=255)
                                                                                                                                        tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc              >  2:1360157/1‑150 (MQ=255)
                                                                                                                                        tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTGTTGACAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc              >  2:6350645/1‑150 (MQ=255)
                                                                                                                                        tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCACAACCATCCCCCCATGGGCAGAATATTTAATTTCGGATTAGTTGGGAAGTTAAGGGACTTTTTAAAGTGATGGTGGTGGGGGAAGGATTCGAACCGTCGAAGTCGATGACGGc              >  1:8215624/1‑150 (MQ=18)
                                                                                                                                         cGCTGGCGCTCGAGACGAACCTTTGTCGACGCTTCTCATCCTTCCCCGCATGGGCATCATATTTAATGGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa             <  1:8971035/150‑1 (MQ=18)
                                                                                                                                            tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt          >  2:3415994/1‑150 (MQ=255)
                                                                                                                                             ggCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGt                                                           <  1:3644645/100‑1 (MQ=255)
                                                                                                                                               cgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTa       <  2:4200878/150‑1 (MQ=255)
                                                                                                                                               cgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGAAGGCAGATTTa       >  1:411818/1‑150 (MQ=255)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa     >  2:3040101/1‑150 (MQ=255)
                                                                                                                                                  tCGAGTCGAACCTTGGTCGAAGCATCTCAACCATACCCGCATGGGCAGAATATTTAATTGCGGATTAGTTGGGTAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAg    >  1:4564071/1‑150 (MQ=21)
                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCAACGAAGTAGAAGACGGCAGATGTACAGta  >  1:7646021/1‑149 (MQ=18)
                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCAAGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  >  1:1204712/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287001‑1287298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: