Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,228 G→C 10.9% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,2280GC10.9% 1591.3 / 66.1 503intergenic (‑189/+179)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base G (147/293);  new base C (47/13);  total (197/306)
Fisher's exact test for biased strand distribution p-value = 4.84e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 515 total aligned reads displayed.

ATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAG  >  NC_000913/1351079‑1351376
                                                                                                                                                     |                                                                                                                                                     
aTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCag                                                                                                                                                       <  2:8510230/150‑1 (MQ=255)
 tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                                      <  2:5106118/150‑1 (MQ=255)
  cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCagag                                                                                                                                                     <  1:6811264/150‑1 (MQ=255)
         ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAg                                                                                                                                              <  1:5090929/150‑1 (MQ=255)
           cgtcgtTTTAGTAAACAGTACGAACAGATAACCGGTTATTATACTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAggg                                                                                                                                            <  2:9488293/150‑1 (MQ=255)
            gtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCTAACCCTAAACCTCTACCCCGAGGGGCgttggg                                                                                                                                           >  2:3171253/1‑145 (MQ=255)
             tcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACTCCCCAGAGGGGCGAGGGGa                                                                                                                                          >  2:4232402/1‑150 (MQ=255)
              cgtTTTAGTACACCGTACGAACAGCTACACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgggtta                                                                                                                                         <  2:4529382/150‑4 (MQ=39)
              cgtTTTAGTAAACAGTACGAACAGATCAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCGCCCCTCTGGGGAGAgggtta                                                                                                                                         <  2:8647820/150‑4 (MQ=255)
              cgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAc                                                                                                                                         <  1:8091206/150‑1 (MQ=255)
              cgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAc                                                                                                                                         <  2:4640696/150‑1 (MQ=255)
               gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCACACCCAAAACCACACCCCACAGGGGCGCGGGGccc                                                                                                                                        >  1:3568179/1‑150 (MQ=255)
                     gTAAACAGTACGAACAGATAACCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtg                                                                                                                                  <  1:4823043/150‑1 (MQ=255)
                      tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                                                                 <  2:5763814/150‑1 (MQ=255)
                      tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                                                                 <  1:8299787/150‑1 (MQ=255)
                       aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAAACCACTCCCCACAGGGGCGAGGGGACCCTaatttg                                                                                                                                >  2:3620716/1‑145 (MQ=255)
                         aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                                                                             <  2:4961547/150‑1 (MQ=255)
                           aGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCCCCCCCAAACCCACACCCCCGCGGGGCGGGGGGGCCGTAGTTTGCcaa                                                                                                                           >  1:1805243/1‑150 (MQ=255)
                           aGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                                                                           <  1:9644078/150‑1 (MQ=255)
                            gTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAat                                                                                                                          >  2:1628651/1‑150 (MQ=255)
                              aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAatat                                                                                                                        <  2:691706/150‑1 (MQ=255)
                                gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCTCCCCCTAAACCACTCCCCACAGGGGCGAGGGGCCCCGATTTTGCAAATATTg                                                                                                                      >  1:370775/1‑150 (MQ=255)
                                gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCACCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAttt                                                                                                                      >  2:5339021/1‑149 (MQ=255)
                                    aGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCGCACCCCAGAGGGGCGCGGGGACCGGATTGTGCAAATAATTTTAc                                                                                                                  >  1:8586697/1‑150 (MQ=255)
                                    aGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCAATCCCCAGAGGGGCGCGGGGACCGGATTGTGCAAAAATTGTTAc                                                                                                                  >  1:7207936/1‑150 (MQ=255)
                                      aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAccc                                                                                                                <  2:9880981/150‑1 (MQ=255)
                                        aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCa                                                                                                              >  1:7524034/1‑150 (MQ=255)
                                        aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCCAACCCAAAACCCACACGGGCGCGGGGCCCCGATTGTGCCAAAATTGTTTCCCCa                                                                                                              >  2:1801995/1‑150 (MQ=255)
                                          aCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCGCACCCAAACCCACACCCCAGAGGGGCGAGGGGTCCGTATTTTGGAAAAATTGTTAACCCAGc                                                                                                            >  1:422336/1‑150 (MQ=255)
                                            ggTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCCCACCCAAACCCACACCCCACAGGGGCGCGGGGGCCCGATTGTGCACATATTGTTAcccccccaa                                                                                                          >  2:2153246/1‑145 (MQ=255)
                                             gTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaac                                                                                                         <  1:5617820/150‑1 (MQ=255)
                                             gTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAAACCAAACCCCAGAGGGGCGAGGGGTCCGTATTGTGCACAAAATGTTAACCCACcaaa                                                                                                         >  2:9662857/1‑149 (MQ=255)
                                                attaaAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                      <  2:9720553/150‑1 (MQ=255)
                                                 ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCTCACCCTAACCCTCTCCGCAGAGGGGGGTGGGGTCCGTTTTGTGGTTATTTTGTTAGCCCAGcaacaatc                                                                                                     >  2:5688447/1‑148 (MQ=255)
                                                 ttatAATCAACCTGGCTGTGAGTAGCTAAACTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCATATCCCCACACCCTAACCCACTCCCCACAGGGGCGAGGGGACCGTATTGTCCAAATATTGTTACCCCACCaacaaac                                                                                                     <  2:9255036/150‑1 (MQ=255)
                                                  tataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCCACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCGCACCCCAACCCACACACCAGGGGGGGGAGGGGGCCCGATAGTGCCAAAAATTGTAACCCCCCaacaaaca                                                                                                    >  1:7671951/1‑150 (MQ=255)
                                                   ctaATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAg                                                                                                   <  2:10018798/149‑1 (MQ=255)
                                                    taATCAACCTGGCTGTGAGTAGCTATTGTGGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                  <  1:1085657/150‑1 (MQ=255)
                                                    taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                  <  2:8793531/150‑1 (MQ=255)
                                                      aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCCCACCCGAACCCACACCCCACAGGGGCGCGGGGGCCCGATTGTGCCAAAAATGTTACCCCCCCcacaaacacccg                                                                                                >  1:2304439/1‑146 (MQ=255)
                                                      aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTc                                                                                               <  2:7584118/150‑1 (MQ=255)
                                                       tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCCCCCTAACCCTCTCCCCAGAGGGGCGCGGGGACCGTCTTGTGCAAATATTGTTTCCCCCCCCACCAACAGGcgc                                                                                               >  2:600323/1‑148 (MQ=38)
                                                       tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTc                                                                                               <  2:4761364/150‑1 (MQ=255)
                                                       tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTc                                                                                               <  2:2327491/150‑1 (MQ=255)
                                                       tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAAACAAACCCCCCAGGGGCGAGGGGGCCGGAATGTGCAAAAATTTTTAACCCCGCAACAAAAACGCTc                                                                                               >  1:8706599/1‑150 (MQ=255)
                                                          aCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATa                                                                                            <  1:2132174/150‑1 (MQ=255)
                                                             tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAg                                                                                         <  1:9717214/150‑1 (MQ=255)
                                                              ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                        <  1:7309345/150‑1 (MQ=255)
                                                              ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                        <  2:2583315/150‑1 (MQ=255)
                                                               gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGcc                                                                                       <  2:6718297/150‑1 (MQ=255)
                                                                cTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGccc                                                                                      <  1:8422909/150‑1 (MQ=255)
                                                                 tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCACACCCCAGATGGGCGAGGGGACCCTAATGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATAAAGcccc                                                                                     >  2:552480/1‑150 (MQ=255)
                                                                  gtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCt                                                                                    <  1:1931480/150‑1 (MQ=255)
                                                                               gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                                                                       <  1:1413119/150‑1 (MQ=255)
                                                                               gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                                                                       <  1:1218996/150‑1 (MQ=255)
                                                                               gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                                                                       >  2:3384979/1‑150 (MQ=255)
                                                                                ttGCCAGGTCCGACCGGAGCTGGCTGCGGCTGGGGGGGCGCTTTTCCCCTCTCCCTAACCCTCTCGCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATTCAGCCCCTAACCCTTTCATGGc                                                                      <  1:4006986/150‑1 (MQ=39)
                                                                                ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc                                                                      <  2:4337533/150‑1 (MQ=255)
                                                                                gtGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc                                                                      <  2:1314274/149‑1 (MQ=255)
                                                                                    cAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATg                                                                  >  2:8218755/1‑150 (MQ=255)
                                                                                    cAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCCAACCCGCTCCCCACAGGGGCGAGGGGGCCGTGTTGTGCCAATATTGTTTACCCAGCCCCAAACAGGCGCATAACGCCCCTAACCCGTTCAGGGCgcgg                                                                  >  2:1121159/1‑147 (MQ=255)
                                                                                     aGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCGAACCCTCTCCCCCGAGGGGCGAGGGGCCCGTATTGTGCGAATAATGTTAACCCAGCAACAAACACGGTCAGAAAGCCCCTAACCCTGTAATGGCgcggg                                                                 >  2:245170/1‑146 (MQ=255)
                                                                                      ggTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGc                                                                <  2:2768244/150‑1 (MQ=255)
                                                                                         ccGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTgg                                                             <  2:385730/150‑1 (MQ=255)
                                                                                               ggAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGt                                                       <  1:5820754/150‑1 (MQ=255)
                                                                                                gAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt                                                      <  1:8853246/150‑1 (MQ=255)
                                                                                                gAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt                                                      <  2:26585/150‑1 (MQ=255)
                                                                                                gAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt                                                      <  1:1570698/150‑1 (MQ=255)
                                                                                                   cAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAg                                                   <  1:6084746/150‑1 (MQ=255)
                                                                                                   cAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAg                                                   <  1:6381487/150‑1 (MQ=255)
                                                                                                   cAGGCTGCGGCAGGGGGGGCGCTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGa                                                  <  1:278782/150‑1 (MQ=255)
                                                                                                      gCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCCCACCCCAGAGGGGCGAGGGGACCGGATTGTGCAAAAATTGTTACCCCACCAACAAACACGCTCAAAAAGCCCCAAACCCAATAAACGCGAAGGCTGGGACGGTTCAGAcc                                                >  2:1975061/1‑150 (MQ=25)
                                                                                                       cTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCt                                               <  1:4385524/150‑1 (MQ=255)
                                                                                                          cGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGc                                            <  1:6701441/150‑1 (MQ=255)
                                                                                                               gggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAat                                       <  1:337942/150‑1 (MQ=255)
                                                                                                               gggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAat                                       <  2:5802079/150‑1 (MQ=255)
                                                                                                                ggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAata                                      <  1:2553124/150‑1 (MQ=255)
                                                                                                                     gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                 <  1:3134995/150‑1 (MQ=255)
                                                                                                                     gcgcTTTTCCCCTCACCCTAACCCTCACCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATAAAGCCCCTAACCCTATCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtcac                                 >  2:7200644/1‑148 (MQ=255)
                                                                                                                       gcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCa                               <  2:7913414/150‑1 (MQ=255)
                                                                                                                         ttttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGc                             >  1:9177681/1‑150 (MQ=255)
                                                                                                                            tCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCAcc                          <  1:7922711/150‑1 (MQ=255)
                                                                                                                            tCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCAcc                          <  1:5385053/150‑1 (MQ=255)
                                                                                                                            tCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCAcc                          <  1:4677682/150‑1 (MQ=255)
                                                                                                                              cccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGt                        >  2:3026070/1‑150 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtc                       >  1:9004581/1‑150 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtc                       >  1:6582777/1‑150 (MQ=255)
                                                                                                                                  cACCCTACCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTcc                    <  1:3914568/150‑1 (MQ=255)
                                                                                                                                    cccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCAt                  <  2:357832/150‑1 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATg                 >  1:7038243/1‑150 (MQ=255)
                                                                                                                                         aCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtttt             <  2:7797513/150‑1 (MQ=255)
                                                                                                                                         aCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtttt             <  2:6260029/150‑1 (MQ=255)
                                                                                                                                           ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca           >  2:7478984/1‑150 (MQ=255)
                                                                                                                                           ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca           >  1:8666254/1‑150 (MQ=255)
                                                                                                                                            ctctCCCCCGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGTCCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac          <  2:904330/150‑1 (MQ=255)
                                                                                                                                            ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac          <  1:7428984/150‑1 (MQ=255)
                                                                                                                                            ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac          >  1:1606614/1‑150 (MQ=255)
                                                                                                                                             tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc         >  2:6514205/1‑150 (MQ=255)
                                                                                                                                             tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGGTACCCCAGCAACAAACAGGCTCATACAGCCCCGAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc         <  1:10003926/150‑1 (MQ=255)
                                                                                                                                               tcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcaccac       <  1:4077081/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAG  >  NC_000913/1351079‑1351376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: