Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,228 C→A 9.5% intergenic (‑24/+284) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2280CA 9.5% 1296.0 / 16.4 485intergenic (‑24/+284)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (155/248);  new base A (64/3);  total (231/254)
Fisher's exact test for biased strand distribution p-value = 2.66e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 497 total aligned reads displayed.

ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGG  >  NC_000913/2177079‑2177377
                                                                                                                                                     |                                                                                                                                                      
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                        <  1:88551/150‑1 (MQ=255)
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                        <  1:271939/150‑1 (MQ=255)
  gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGa                                                                                                                                                      >  2:3183699/1‑150 (MQ=255)
   cAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGAt                                                                                                                                                     >  2:927906/1‑150 (MQ=255)
     ggTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAttt                                                                                                                                                   <  2:6368527/150‑1 (MQ=255)
      gTTGGCAGCGGTTTCTACCACCACTTTAATCGTTTAGAGATTGTGAATATTGAATGACGTAACAGCATAAACGCCGCGCTTTGCGTTTTTCAGAATATGCTTTGTCTATAACAAGTACATTTTCATATACTGTCGTTTTTTTTTGAttta                                                                                                                                                  >  1:6820124/1‑149 (MQ=255)
            aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                            >  2:9838845/1‑150 (MQ=255)
            aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                            <  1:361102/150‑1 (MQ=255)
               ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                        >  2:5540555/1‑150 (MQ=255)
                gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAt                                                                                                                                       <  2:4662945/150‑1 (MQ=255)
                     tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                  <  2:2421351/150‑1 (MQ=255)
                        caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                               <  1:1769461/150‑1 (MQ=255)
                        caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAattat                                                                                                                               >  1:7668263/1‑150 (MQ=255)
                        caccacGTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                               <  1:9247049/150‑1 (MQ=255)
                         accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                              <  2:7047643/150‑1 (MQ=255)
                            acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                           <  1:9757446/150‑1 (MQ=255)
                            acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                           <  1:3228451/150‑1 (MQ=255)
                             cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                          <  2:893323/150‑1 (MQ=255)
                               tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAAATAtttttttt                                                                                                                        >  1:1013358/1‑150 (MQ=255)
                                gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATtttttttt                                                                                                                       >  1:3080267/1‑150 (MQ=255)
                                 cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                      <  1:4506309/150‑1 (MQ=255)
                                 cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                      <  1:1726202/150‑1 (MQ=255)
                                   tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATAAAATGAAAATATTTTTTTTTaaa                                                                                                                    >  1:3464862/1‑150 (MQ=255)
                                   tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTaaa                                                                                                                    >  2:9086921/1‑150 (MQ=255)
                                     gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAata                                                                                                                  >  2:487043/1‑150 (MQ=255)
                                       ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                <  1:3946024/150‑1 (MQ=255)
                                        tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                               >  1:663664/1‑150 (MQ=255)
                                         cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                              <  2:5761676/150‑1 (MQ=255)
                                          gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                             >  1:9997817/1‑150 (MQ=255)
                                          gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCgag                                                                                                             >  2:221084/1‑150 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGAt                                                                                                     >  1:8311688/1‑150 (MQ=255)
                                                   aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                    <  2:8341625/150‑1 (MQ=255)
                                                   aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                    <  2:3974900/150‑1 (MQ=255)
                                                   aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                    <  1:2248952/150‑1 (MQ=255)
                                                   aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTATCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                    <  2:2236474/150‑1 (MQ=255)
                                                     tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTTTTTTTTTTTAAATATAGAgataacgataac                                                                                                  >  1:3847883/1‑150 (MQ=255)
                                                        tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAAGAAAATATTTTTTTTTAAATATAGAGATAACCATCACaaa                                                                                               >  2:1802395/1‑150 (MQ=255)
                                                         gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                              >  2:4107606/1‑150 (MQ=255)
                                                         gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                              <  1:737167/150‑1 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                             <  1:1358710/150‑1 (MQ=255)
                                                           aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                            <  2:9526518/150‑1 (MQ=255)
                                                             gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                          <  2:3501524/150‑1 (MQ=255)
                                                             gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                          <  2:7587074/150‑1 (MQ=255)
                                                              ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGTAATTATTTTTTTTTAAATATAGAGATTAAGATCACAAAAACGAc                                                                                         >  1:8626974/1‑150 (MQ=255)
                                                                ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACaa                                                                                       >  2:4950754/1‑150 (MQ=255)
                                                                   aCCGCATAACGGCGGGGGTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTGTTTTTAAATATGGAGATAACGATCACAAAAACGACAatat                                                                                    <  2:8177793/150‑1 (MQ=255)
                                                                    ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATg                                                                                   >  2:1963646/1‑150 (MQ=255)
                                                                      gCATAACCGCCGCGCTGTGCTTTGTTCAGCATCGGATTTGTCGATACCACTTACATTTTAATATCCTTTCTTTTGTTTTCTATTTCAAATTATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGaa                                                                                 <  2:8747413/150‑1 (MQ=255)
                                                                        aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaaa                                                                               >  2:4058276/1‑150 (MQ=255)
                                                                           cccgccgCGCTGTGCGTTGTTCAGCATCTGGTTTGTCGATACCACGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatta                                                                            <  2:3549775/149‑1 (MQ=255)
                                                                            ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                           <  2:5085541/150‑1 (MQ=255)
                                                                            ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTCAAAAAATAATGAAAATATTTTTTTTTTAAAATATAGATAAAGAAAAAAAAAAAAACAAAAATAAAaatat                                                                           >  1:2427392/1‑150 (MQ=255)
                                                                             cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATGAAAATATTTTTTTTTTAATATAGAGATAACGATAACAAAAAACAAAATATGAAAattatt                                                                          >  1:6046129/1‑150 (MQ=255)
                                                                             cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGACGATACCACTAACATTTTCAAATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                          <  2:2919524/150‑1 (MQ=255)
                                                                              gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGTTTTCAAAATATAATTAAAATATTTTTTTTTTAATATAGAGATAAACATCACAAAAAAGAAAAAAATAAAaatatta                                                                         >  1:1947109/1‑149 (MQ=255)
                                                                              gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTTTTTTGGATTTCAAAATATAATTAAATTATTTTTTTTTAAATAACGAGATAAAGATCACCAAAAAGACAATATTTAAattatta                                                                         >  1:7095261/1‑149 (MQ=255)
                                                                                cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                       <  2:7024381/150‑1 (MQ=255)
                                                                                 gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTTTAATATAGTGTTAACGATAAAAAAAAAGACAATATTAAAAATATTagag                                                                      >  1:5612086/1‑150 (MQ=255)
                                                                                       tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGa                                                                >  2:5599261/1‑150 (MQ=255)
                                                                                       tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGa                                                                <  2:6177645/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGCATGTGCTTTGTCTATACCCCTTACATTTTAATATCCGGTGTTTTGTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGAGATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  1:1126774/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  2:6501853/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  2:1492183/150‑1 (MQ=255)
                                                                                              ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAAAGATCAAAAAAACGACAATATTAAAATTATTAGAGGAGTGAAAGGCaa                                                         >  2:2519216/1‑150 (MQ=255)
                                                                                                cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                       <  2:7813164/150‑1 (MQ=255)
                                                                                                   cATCTGTTTTGTCGATCCCACTTACTTTTTAATATCGTTTCTTTTTTTTTCTATTTAAAAATATAATGAATTTTTTTTTTTTTAAATTTCGAGATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                    <  1:4609002/150‑1 (MQ=255)
                                                                                                   cATCTGCTTTGTCGACACCACGTACATTTTCATATCCTGTCTTTTTTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                    <  1:6183616/150‑1 (MQ=255)
                                                                                                    aTCTGCTTTGTCGATACCACGAACTTTTTCATATCGTGTCGTTTTTTTTCTATTTAAAAATATATTCAATTTATTTTTTTTTAAATATCGTTATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                   <  1:437602/150‑1 (MQ=255)
                                                                                                     tgtgtttttTCTATACCACTTATTTTTTAATTTCATTTGTTTTTTTTTTTTTTAAAAATTTTAATTAATTTTTTTTTTTTTTATTATTTATATAACTAAAAAAAAAACGAAAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                  <  1:4218839/145‑1 (MQ=255)
                                                                                                      cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                 <  2:5192580/150‑1 (MQ=255)
                                                                                                         cTTTGTCGATACCACGTACTTTTTCATATCCTGTCTTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                              <  2:4316237/150‑1 (MQ=255)
                                                                                                             gTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAAAAATGAAATTATTTTTTTTTAAATAAAGATATAACGATAAAAAAAAACACAATATTAAAAATATTAGAGGAGTGAAACGCAAAAAAAAGGCCACCCg                                          >  2:3724157/1‑150 (MQ=255)
                                                                                                                taTACCACTTACTTTTTCATATCCTTACGTTTTTTTTCGATTAAAAAATTTAATGAAATTTTTTTTTTTTAAATATCGAGATATCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                       <  1:6463270/149‑1 (MQ=255)
                                                                                                                taTACCACTTAAATTTTACTATCTTTTCTTTTTTTTTCGATTAAAAATTATAATCAATTTTTTTTTTTTTAAATATCTATATAACCATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                       <  1:8100229/149‑1 (MQ=255)
                                                                                                                    ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAGATGTAAATTATTCGAGGGGTGAAAGGCAAAAAAAAGGCCGCCCCATAGGGa                                   >  2:6802974/1‑150 (MQ=255)
                                                                                                                     cccGTAAATTTTCATTTCGTTTCTTTTTTTTCCTTTTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCTTGGAGTGAACGGCAAAAAAACGGCCTCCCGATAGGGaa                                  <  1:5185951/148‑1 (MQ=255)
                                                                                                                       cGTACATTTTCATATCCTGTCTTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                <  2:3623212/150‑1 (MQ=255)
                                                                                                                        ggaCATTTTCATATCCTGTCGTTTGTTTACTATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                               <  2:5946768/148‑1 (MQ=255)
                                                                                                                        gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGTGGGGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                               >  2:7449563/1‑150 (MQ=255)
                                                                                                                           cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                            <  2:8282253/150‑1 (MQ=255)
                                                                                                                           cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                            <  1:5675040/150‑1 (MQ=255)
                                                                                                                           cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                            <  2:9129210/150‑1 (MQ=255)
                                                                                                                            aTTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATACTGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                           <  1:581923/150‑1 (MQ=255)
                                                                                                                                tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                       <  2:1270627/150‑1 (MQ=255)
                                                                                                                                tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                       <  2:1384310/150‑1 (MQ=255)
                                                                                                                                  atatCCTGTCTTTTGTTTTCTATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                     <  2:855385/150‑1 (MQ=255)
                                                                                                                                   tatCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCAAAAAAAAGACAATATGAAAATTATTGGAGGGGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                    >  2:7267390/1‑150 (MQ=255)
                                                                                                                                      ccTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                 <  1:599656/150‑1 (MQ=255)
                                                                                                                                         gTCTTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg              <  1:6350477/150‑1 (MQ=255)
                                                                                                                                         gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg              >  2:1906913/1‑150 (MQ=255)
                                                                                                                                           cGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt            <  2:8830550/150‑1 (MQ=255)
                                                                                                                                            gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt           >  1:7919316/1‑150 (MQ=255)
                                                                                                                                             tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtgtt          >  1:9887520/1‑150 (MQ=255)
                                                                                                                                                gtttTCTATTAAAAATTATACTGATTTTTTTTGTTTTTAATTTTCGAGATAACGATAACAAACACGACAATATGAAAATTATTCGAGGTGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt       <  1:9118739/150‑1 (MQ=255)
                                                                                                                                                 ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg      >  1:1426245/1‑150 (MQ=255)
                                                                                                                                                  tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc     <  2:1013358/150‑1 (MQ=255)
                                                                                                                                                  tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc     >  2:1644104/1‑150 (MQ=255)
                                                                                                                                                   ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc    >  2:2760502/1‑150 (MQ=255)
                                                                                                                                                    tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg   >  1:4876878/1‑150 (MQ=255)
                                                                                                                                                     cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGGGCGCGGGTTTTTATGCCgg  >  1:9582504/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGG  >  NC_000913/2177079‑2177377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: