Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,185,429 T→G 21.3% intergenic (‑128/+95) thiM ← / ← rcnR hydoxyethylthiazole kinase/transcriptional repressor of rcnA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,185,4290TG21.3% 1241.9 / 137.4 575intergenic (‑128/+95)thiM/rcnRhydoxyethylthiazole kinase/transcriptional repressor of rcnA
Reads supporting (aligned to +/- strand):  ref base T (123/319);  new base G (131/2);  total (254/321)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 609 total aligned reads displayed.

AACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGT  >  NC_000913/2185280‑2185578
                                                                                                                                                     |                                                                                                                                                     
 aCCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGttt                                                                                                                                                      <  1:4982527/150‑1 (MQ=255)
 aCCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGttt                                                                                                                                                      <  2:2611384/150‑1 (MQ=255)
 aCCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGttt                                                                                                                                                      <  2:7473180/150‑1 (MQ=255)
 aCCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGttt                                                                                                                                                      <  2:6086436/150‑1 (MQ=255)
   ccAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTgg                                                                                                                                                    >  2:8051372/1‑150 (MQ=255)
   ccAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTgg                                                                                                                                                    >  2:3492298/1‑150 (MQ=255)
     agcagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGtt                                                                                                                                                  <  2:3963660/150‑1 (MQ=255)
      gcagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTg                                                                                                                                                 <  1:2284168/150‑1 (MQ=255)
      gcagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTg                                                                                                                                                 >  2:8382857/1‑150 (MQ=255)
        agGTCGACTTACATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCg                                                                                                                                               <  1:1084432/150‑1 (MQ=255)
         ggTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCga                                                                                                                                              <  2:112255/150‑1 (MQ=255)
           tCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgatg                                                                                                                                            <  2:6744895/150‑1 (MQ=255)
              aCTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATa                                                                                                                                         <  2:1775959/150‑1 (MQ=255)
                 tGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgatttatcat                                                                                                                                      <  2:4812960/150‑8 (MQ=255)
                 tGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAt                                                                                                                                      <  2:1916653/150‑1 (MQ=255)
                  gCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATaaaaa                                                                                                                                     >  1:6292669/1‑148 (MQ=255)
                  gCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATc                                                                                                                                     <  1:3110927/150‑1 (MQ=255)
                   cATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGGGGTTGCGCTGATAAAACg                                                                                                                                    >  2:9718400/1‑150 (MQ=255)
                        tttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCagag                                                                                                                               >  1:8255006/1‑150 (MQ=255)
                        tttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGGGGGTGCGCTGATAAATAGCagag                                                                                                                               >  2:4490424/1‑150 (MQ=255)
                          tGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAgagga                                                                                                                             <  1:7225407/150‑1 (MQ=255)
                            catatGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGaggagg                                                                                                                           <  2:4602966/146‑1 (MQ=255)
                             tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGAGTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGTAGGGCACCCCGAGTCGTTTGGTTTGGTTGTTAAATAGGAGtggaggt                                                                                                                          >  1:1657127/1‑149 (MQ=255)
                               cTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGGGGTGTTAAATCGCAGAGGAGGATg                                                                                                                        >  1:4709343/1‑150 (MQ=255)
                                   cATCACGTGAAGAAGCACTGACCTGGTGGTCCGTGACGTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAGTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTaa                                                                                                                    <  2:5718671/150‑1 (MQ=255)
                                   cATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGGTAAATCGCAGAGGAGGATGGTag                                                                                                                    >  1:9559401/1‑149 (MQ=255)
                                    aTAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAAt                                                                                                                   <  1:9966343/150‑1 (MQ=255)
                                     tAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGAGGGGGATGGGAATg                                                                                                                  >  1:3826592/1‑150 (MQ=255)
                                        cGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTcc                                                                                                               <  1:5787995/150‑1 (MQ=255)
                                         gTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCa                                                                                                              <  1:2838036/150‑1 (MQ=255)
                                          tGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAt                                                                                                                                      >  2:7314486/1‑125 (MQ=255)
                                          tGAAGAAGCAATGACCTGGTGGTCCATGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGGTGGGATGAGAAAAAGCAGAGGAGGTTGGTTATGTCCac                                                                                                             >  1:3640457/1‑149 (MQ=255)
                                           gaagaaGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAgc                                                                                                            <  2:5819538/150‑1 (MQ=255)
                                           gaagaaGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGTTAAATCGCAGAGGAGGATGGGTATGTCCCgc                                                                                                            >  1:9488606/1‑150 (MQ=255)
                                                   aaTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATAGCAGAGGAGGGTGGTAATGTCCAGCGCAcgcgg                                                                                                    >  1:7404543/1‑149 (MQ=255)
                                                    aTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGATAAATAGCAGAGGAGGATGGTAATGTCCAGCGCAcgcggt                                                                                                   >  1:3213087/1‑148 (MQ=255)
                                                         cTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTaaa                                                                                              <  1:145574/150‑1 (MQ=255)
                                                         cTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTaaa                                                                                              <  2:3081704/150‑1 (MQ=255)
                                                          tggtggTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAAc                                                                                             <  1:2056260/150‑1 (MQ=255)
                                                          tggtggTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAAc                                                                                             <  1:4039210/150‑1 (MQ=255)
                                                          tggtggTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAAAAGCCGCGGGGGATGGTAAAGTACAGCGCACGCGTTTTaaaa                                                                                             >  1:7726372/1‑149 (MQ=255)
                                                            gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                           <  1:709788/150‑1 (MQ=255)
                                                            gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                           <  1:8382857/150‑1 (MQ=255)
                                                            gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                           <  2:2104778/150‑1 (MQ=255)
                                                              ggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGAATATAAAACGCAAAGGAAGATTGTAAATTCAAGCCCACGCCTTTTaaaaaaag                                                                                         >  1:7951162/1‑145 (MQ=255)
                                                                  cGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAACGCAGAGGAGGATTGTAATGTCCACCGCACGCGTTGTAAACGAGGaaaa                                                                                     >  2:3303541/1‑150 (MQ=255)
                                                                    tGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAAc                                                                                   <  2:3140102/150‑1 (MQ=255)
                                                                    tGACTTCCCTACGCTGACATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAAc                                                                                   <  1:2966827/150‑1 (MQ=255)
                                                                      aCTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACgg                                                                                 <  2:2844218/150‑1 (MQ=255)
                                                                        ttCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGGGAGGATAAATAGCAGGGGAGGATGGTAATGTCCCCCGCACCCCGTGTAAACGAGGaaaaaacgcc                                                                               >  2:9422092/1‑145 (MQ=255)
                                                                        ttCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCCATGATAAATAGCCGAGGAGGATGGTAATGTCCAGCGCCCGCCTTGTAAACCAGGAAAAACGGCa                                                                               >  2:8823249/1‑150 (MQ=255)
                                                                          cccTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGGTGATAAAAAGCAGAGGAGGGTTGTTATGTGCAGCGCCCGCCTTGTTAACGAGGGAAAAAGGCAgg                                                                             >  1:5174456/1‑149 (MQ=255)
                                                                           ccTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATAGCCGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGtt                                                                            >  2:1356432/1‑150 (MQ=255)
                                                                            cTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATAGCAGAGGAGGGTGGTAATTTCCCGCGCACGCGTTGTAAACGAGGAAAAAAGGCCGttt                                                                           >  1:1323143/1‑150 (MQ=255)
                                                                            cTACGCTGGAATTATCCAGATCAGGTTATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATTATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGttt                                                                           <  2:4363134/150‑1 (MQ=255)
                                                                                gCTGGCATTATCCAGATCTGGTTATACGGTTATTTCTCCGCCTTCACGCAGAAGGGCACCCCGTGTCGTTTGGTTGCTATGATAAATCGCTGAGGTGGTTGGTAATGTCCAGCGCACGCGTTGTAAACGGGGAAAAACGGCAGTTTTACa                                                                       <  1:2511982/150‑1 (MQ=255)
                                                                                  tGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGGTGATAAAACGCAGAGGAGGATGGTAATGTCCAGCGCACGCGGTGTAAACGAGGAAAAAAGGCCGTTTTACaaa                                                                     >  1:413930/1‑149 (MQ=255)
                                                                                    gCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATcg                                                                   <  2:5515668/150‑1 (MQ=255)
                                                                                     cATTATCCAGATCTGGTGATACGGTTATTTCTCAGCCTTCACGCTGACGGGCACCCCGAGTCGTTTGTTTGCGATGATAAATCGCAGAGGAGGATGGTAATGCCCAGCGCACGCGTTGTAAACGAGGAAACACGGCAGTTTTACAATcgc                                                                  <  1:9208023/150‑1 (MQ=255)
                                                                                     cATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATcgc                                                                  <  2:8472471/150‑1 (MQ=255)
                                                                                       ttATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGt                                                                <  2:6560156/150‑1 (MQ=255)
                                                                                              gATCTGGTGATACGGGTATTTCTCAGCCTCCACGCTGAAGGGCACCCCGAGTCGTTTGGTTGCGATTATAAATCGCATTGGAGGTTGGTAATGTCCCGCGCACGCGTTGTAAACGTGGAAAAACGGCAGTTTTACAATCGCGTTAATaaa                                                         <  1:4411198/150‑1 (MQ=255)
                                                                                              gATCAGGTGATACGGGTATTTCTCAGCATTCACGCAGAAGGGCACCCCGTGTCGTTTGGTTGCTATGATAAATCGAAGAGGTGGATGGTATTGTCCAGCGCACGCTTTTTAAACGTGGAAAAACGGCAGTTTTACAATCGCGTTAATaaa                                                         <  1:261258/150‑1 (MQ=255)
                                                                                                tCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGGGGGTTCGCTGATAAAACCCACAGGAGGCTGGTAATGTCCCGCGCACCCGGTGTAAAACAGGACAAACCGCCCTTTTACAATCGCGGTAATaaaat                                                       >  2:954080/1‑148 (MQ=255)
                                                                                                  aGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATGATAAAAAGCAGAGGAGGGTGGTAATGTCCAGCGCACGCGTTGTAAAAGAGGAAAAAACGCAGTTTTACAAACGCGTTAATAAattat                                                     >  1:6474118/1‑150 (MQ=255)
                                                                                                  aGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAattat                                                     <  1:6188089/150‑1 (MQ=255)
                                                                                                   ggTGATAGGGGTTTTTCTCTGGCTTCTCGCAGTTGTGCTCCGCGTGTGGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAattatt                                                    <  2:7356073/150‑1 (MQ=255)
                                                                                                   ggTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTGGATGTTAAAAAGCAGAGGAGGATGGTAATGTGAAGCGCCCCCGTTGTAAAACAGGAAAAAAGGCCATTTTAAAAACGCGGTAAAAAaatatt                                                    >  1:5976576/1‑150 (MQ=255)
                                                                                                    gTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTAttt                                                   <  2:8215088/150‑1 (MQ=255)
                                                                                                      gATACGGTTATTTCTCAGCCTTCACGCTTATGGGCACCCCTAGTCTTTTGGTTGCTATTTTAAATCGCATTGGAGTATGGTAATGTCAAGCGCACGCTTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGa                                                 <  1:890188/150‑1 (MQ=255)
                                                                                                      gATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATATCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGa                                                 <  2:3739569/150‑1 (MQ=255)
                                                                                                       aTACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGat                                                <  2:6919021/150‑1 (MQ=255)
                                                                                                        tACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGTAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGata                                               <  1:8730625/150‑1 (MQ=255)
                                                                                                         aCGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATTATAAAAAGCACAGGAGGAGGGTAAATTCCAGCCCACCCGTTGTAAAAGAGGAAAAAAGGCACTTTTACAATAGCGTTAATAAAATATTTGatat                                              >  2:4705673/1‑150 (MQ=255)
                                                                                                          cGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatata                                             <  1:8066847/150‑1 (MQ=255)
                                                                                                           gggTATTTCTCAGCCTTCACGCAGATGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGTGGTGGATGGTATTGTCCAGCGCACGCTTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatatat                                            <  1:4314782/150‑1 (MQ=255)
                                                                                                           gggTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATGATAAAAAGCAGAGGAGGATGGTAATGTCCACCGCACGCGTTGTAAAAGAGGAAAAAAGGCAGTTTTACAATAGCGTTAATAAAATATTTTatatat                                            >  2:478757/1‑150 (MQ=255)
                                                                                                             gTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAAACGCAGAGGAGGCTGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGGTTTACAATCGCGTTAATAAATTAATTtatataata                                          >  2:2501150/1‑147 (MQ=255)
                                                                                                              tATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATTATAAATAGCAGAGGAGGATGGTAAATTACCGCGCAACCGTTTTAAAAGTGGAAAAAACGCAGTTTTAAAAACGCGTTAATAAATTATTTGTTATATTaa                                         >  2:9348950/1‑150 (MQ=255)
                                                                                                                tttCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATc                                       <  2:5144333/150‑1 (MQ=255)
                                                                                                                    tcAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCCTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGTATCCAGc                                   >  2:5285116/1‑150 (MQ=255)
                                                                                                                    tcAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATAGCAGAGGAGGATTGTAATGTCCAGCGCACCCCTTGTAAACGAGGAAAAACGGCACTTTTACAATCGCGGTAATAAATTATTTGATATATGAATACAGc                                   >  2:6029702/1‑150 (MQ=255)
                                                                                                                         cTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCtt                              <  2:7845581/150‑1 (MQ=255)
                                                                                                                         cTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATAGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCtt                              >  2:4171286/1‑150 (MQ=255)
                                                                                                                           tCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCa                            <  2:7064476/150‑1 (MQ=255)
                                                                                                                               gCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAAc                        <  2:7676021/150‑1 (MQ=255)
                                                                                                                               gCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAAc                        <  2:4995741/150‑1 (MQ=255)
                                                                                                                                 aGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGa                      <  2:919142/150‑1 (MQ=255)
                                                                                                                                     gggCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATc                  <  2:6278688/150‑1 (MQ=255)
                                                                                                                                       gCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACCCCTTGTAAACGAGGAAAAACGGCAGTTTTACAAGCGCGTTAATAAAATATTTGATATATGAATACCGCAACTTCAGAACCAAATCCa                >  2:2431501/1‑150 (MQ=255)
                                                                                                                                       gCACCCCGAGTCGTGTGGTTGCGATGATAAATAGCAGAGGAGGCTGGTAATGTCCAGCGCACGCGGTGTAAACCAGGAAAAACCGCAGGTTTACACTCGCGGTAATAAATTATTTGATATATGAATACCGCACCATCAGAACGACATACa                >  2:8985617/1‑150 (MQ=255)
                                                                                                                                        cACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAg               <  1:9000940/150‑1 (MQ=255)
                                                                                                                                        cACCCCCAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGAACTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAg               <  1:6567609/150‑1 (MQ=255)
                                                                                                                                         aCCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGa              <  1:9793393/150‑1 (MQ=255)
                                                                                                                                          ccccGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAt             <  2:2707590/150‑1 (MQ=255)
                                                                                                                                             cGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtctt          <  1:5420230/150‑1 (MQ=255)
                                                                                                                                              gAGTCTTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGATTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtcttc         <  2:1207596/150‑1 (MQ=255)
                                                                                                                                                  cGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCa     <  2:5689112/150‑1 (MQ=255)
                                                                                                                                                   gTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGAGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCAc    <  1:6748557/150‑1 (MQ=255)
                                                                                                                                                    tttGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACg   >  1:5664592/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGT  >  NC_000913/2185280‑2185578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑