Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,177 A→C 15.6% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,1770AC15.6% 579.6 / 22.4 322intergenic (+112/+261)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (56/173);  new base C (39/11);  total (113/209)
Fisher's exact test for biased strand distribution p-value = 2.02e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326
                                                                                                                                                     |                                                                                                                                                      
tACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCa                                                                                                                                                        <  2:5371544/150‑1 (MQ=255)
   tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGTCGCAAGGAGCGCGCAggg                                                                                                                                                    >  2:8596156/1‑150 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCgggggggg                                                                                                                                                  >  1:7613943/1‑150 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCgggggggg                                                                                                                                                  >  1:6864221/1‑150 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCgggggggg                                                                                                                                                  >  1:4642025/1‑150 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCggggg                                                                                                                                                  >  2:3545369/1‑150 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggggg                                                                                                                                                  <  1:8181261/150‑1 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggggg                                                                                                                                                  <  2:9387622/150‑1 (MQ=255)
       ggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcg                                                                                                                                                <  1:1030857/150‑1 (MQ=255)
       ggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcg                                                                                                                                                <  1:300450/150‑1 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                                                                                               <  1:7013987/150‑1 (MQ=255)
          gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGcc                                                                                                                                             >  2:4243649/1‑150 (MQ=255)
           aCTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGCGCGCGCGgggggggggcc                                                                                                                                            >  1:7163135/1‑147 (MQ=255)
           aCTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGccc                                                                                                                                            >  1:982138/1‑149 (MQ=255)
            cTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCaa                                                                                                                                           >  2:2548628/1‑150 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGcccca                                                                                                                                          >  2:4662018/1‑147 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCCGGGGCGGcccca                                                                                                                                          >  2:2022345/1‑147 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAt                                                                                                                                          >  2:5724256/1‑150 (MQ=255)
              ggCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATc                                                                                                                                         <  1:6098870/150‑1 (MQ=255)
              ggCGTATTTAAGAGGATATTTTAAAATTAATTAATGTCATCAGGTCCGAACATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCCCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATc                                                                                                                                         <  1:9085063/150‑1 (MQ=255)
               gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGggggggggggggcaacg                                                                                                                                        >  1:16379/1‑143 (MQ=255)
               gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGggcgggccaaag                                                                                                                                        >  2:437927/1‑143 (MQ=255)
               gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgggccaacc                                                                                                                                        >  1:6060847/1‑143 (MQ=255)
               gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcg                                                                                                                                        >  2:9842596/1‑150 (MQ=255)
                cGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGcccaacac                                                                                                                                       >  2:2661790/1‑144 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgcc                                                                                                                                      <  2:9073160/150‑1 (MQ=255)
                  tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCaaaccccc                                                                                                                                     >  2:8148986/1‑144 (MQ=255)
                  tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccg                                                                                                                                     <  1:5287962/150‑1 (MQ=255)
                    tttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCACAagccgcc                                                                                                                                   >  1:6297284/1‑150 (MQ=255)
                       aaGATGATATTTTAAAATTAATTACTGTCATCAGGTCCGAAACTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGCGGCGGCGATTGgccgccccc                                                                                                                                <  2:7850156/150‑1 (MQ=38)
                         tatgatATTTTAAAATTAATTAATGTCATATGGTCCTAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCTTTGCTTCATATAATCACTGGCGCAAGGTGCGCGCTGGGGGCGGCCAATCGCCGCCGcccc                                                                                                                              <  1:2198266/149‑1 (MQ=37)
                         gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGGGGCCAAAAGCCGCCGcccc                                                                                                                              >  2:7328120/1‑150 (MQ=255)
                          atgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCCAACACcccccccccc                                                                                                                             >  2:958987/1‑150 (MQ=39)
                           tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGCCCCACGCccccccccccc                                                                                                                            >  1:2961616/1‑149 (MQ=255)
                           tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAACccccccccccccc                                                                                                                            >  1:1344052/1‑149 (MQ=39)
                           tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCt                                                                                                                            <  1:4245026/150‑1 (MQ=255)
                             atatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCACACccccccccccccccc                                                                                                                          >  1:9547300/1‑147 (MQ=255)
                               atTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCAc                                                                                                                        <  2:8792405/150‑1 (MQ=255)
                                 tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGGGGCCAAAAGCCGCCCCCCCCCCCAccc                                                                                                                      >  2:3870939/1‑150 (MQ=38)
                                   tAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCAccccc                                                                                                                    <  2:405691/150‑1 (MQ=255)
                                     aaaTTAATTACTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCgg                                                                                                                  <  2:6132778/150‑1 (MQ=255)
                                      aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCCCTAGCCGCCCCCCCCCCCCCCCCCcgg                                                                                                                 >  2:899176/1‑150 (MQ=38)
                                      aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCggg                                                                                                                 <  2:7805371/150‑1 (MQ=255)
                                        ttaattaaTGTCATCAGGTCCGAAAATAACGAGACTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCCGGGGGGGCCCACCGccccccccccccccccccccccccc                                                                                                               >  1:2760381/1‑145 (MQ=255)
                                         taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCCGCGCCGCCCCCCCCCCCCCCCCCCGGcgg                                                                                                              >  1:4067030/1‑148 (MQ=255)
                                         taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCCCCGCCCCCAGCACCCCCCGGctc                                                                                                              >  2:3311792/1‑150 (MQ=255)
                                              aatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCg                                                                                                         <  2:5803969/150‑1 (MQ=255)
                                              aatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCg                                                                                                         <  2:9947908/150‑1 (MQ=255)
                                                tGTCTTCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGaa                                                                                                       <  2:2332464/150‑1 (MQ=255)
                                                  tcatcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACa                                                                                                     <  2:5226969/150‑1 (MQ=255)
                                                             gAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAACGCCGCCGCCCCCAGCCCCCCCCGGCGCTGGCGCACAAAATcgccccg                                                                                          >  2:6236236/1‑147 (MQ=25)
                                                              aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTg                                                                                         <  2:4902454/150‑1 (MQ=255)
                                                                 aTAACGAGATTTTTTCAGTCTCTATGCTTGTGGCGCTCCTGTCTTTTGCTTTGCTTAATATAATCACTGGCGCATGGTGCGCGCTGGGGGCGGCAAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTgcgc                                                                                      <  1:5620901/150‑1 (MQ=17)
                                                                    aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGCGGCCAAACGCCCCCCCCCCCAGCACCCCCCCGCGCAGGCGAACAAAAAACCCCCAGCGCGGt                                                                                   >  2:8054397/1‑150 (MQ=255)
                                                                      gagaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGCCGCCCCCAGCCCCCCCCGGCTCAGGCGAACAAAATAGCCGCGgcgccgggc                                                                                 >  2:3323614/1‑145 (MQ=21)
                                                                       agaATTTTTCAGTCTATCATCCTGTTGCGCTCCTGTCTTTTGCTTTGCTTCATATAACCACGGGCGCATGGCGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGcc                                                                                <  1:5465921/150‑1 (MQ=17)
                                                                          atatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTc                                                                             <  2:1419143/150‑1 (MQ=255)
                                                                            atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCCCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  2:8096052/150‑1 (MQ=255)
                                                                            atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  2:117665/150‑1 (MQ=255)
                                                                             tttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                          <  2:1102568/150‑1 (MQ=255)
                                                                                   tcgctcAGCCTGTTGCGCTCTTTTCTTGTGAATCGCTTCATATAAACCCGGGCGCACGGTGCGCGCTGGGGGCGCCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                    <  1:7793007/147‑1 (MQ=17)
                                                                                   tcactcATCGTGTTGCGCTCCTGTCATTTGCATTGCTCCATATAATCACTGGCGCAAGGCGCGCGCTGGGGGCGGCCAACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                    <  1:2542018/147‑1 (MQ=17)
                                                                                     tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCATGGGGCGGCCAATCGCCCCCGCCCCCTGCACCCCCGGGCTCAGGCGAACAAAATAGCCGCTGCGCGGTGCCCACGGCGTAACCCt                                                                  >  2:7512784/1‑150 (MQ=18)
                                                                                     tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCCCCCCCCCCAGCACCCCCCGGCTCTGGCGAACAAAAACGCCCCGGCGCGGGGCCCACGGCGTATccca                                                                  >  2:1629052/1‑149 (MQ=18)
                                                                                       tcATCGTGTGGCGCTCGTGTCATTTGAATTGCTTCATATAATCACTGGCGCAAGGCGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                <  1:8785106/150‑1 (MQ=17)
                                                                                          tCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                             <  2:2360873/150‑1 (MQ=255)
                                                                                              gTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGGGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAc                                                         <  1:8536070/150‑1 (MQ=255)
                                                                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAcc                                                        <  2:1950924/150‑1 (MQ=255)
                                                                                                 gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCgg                                                      <  2:9827504/150‑1 (MQ=255)
                                                                                                    cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTc                                                   <  2:6353822/150‑1 (MQ=255)
                                                                                                      ccTTTCATGTGAATTGCTTAATATAATCACTGGCGAATGGAGCGCGCTGGGGGCGGCAAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCgg                                                 <  1:3330282/150‑1 (MQ=14)
                                                                                                      ccTGTCATGTGCTTTGCTTCATATAATCACGGGCGCATGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCgg                                                 <  1:196109/150‑1 (MQ=255)
                                                                                                      ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCgg                                                 <  2:5917363/150‑1 (MQ=255)
                                                                                                       cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCGCCCCCCCCAGCCCCCCCCGGCGCTGGCGAACAAAAACGCCGCAGCGCGGTGCCCACCGCGTAACCCATAAGGCTACCCGGTAggg                                                >  2:2510352/1‑150 (MQ=17)
                                                                                                            aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                           <  2:3014061/150‑1 (MQ=255)
                                                                                                             tgtgCATTGCTTCATATACTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                          <  2:420478/150‑1 (MQ=255)
                                                                                                             tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                          <  2:8171884/150‑1 (MQ=255)
                                                                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                         >  1:490329/1‑150 (MQ=255)
                                                                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                        <  2:7048659/150‑1 (MQ=255)
                                                                                                                 cATTGCTTCCTATAATCACGGGCGCCCGGCGCGCGCCGGGGGCGGCCAAGCGCCGCCGCCCCCGGCACCCCCGGGCTCGGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                      <  2:2885699/150‑1 (MQ=14)
                                                                                                                   ttGCTTCATATAAGCACTGGCGCACGGTGCGCGCAGGGGGCGGCCATTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                    <  1:8837134/150‑1 (MQ=255)
                                                                                                                       ttCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCTCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATccc                                <  2:5522569/150‑1 (MQ=255)
                                                                                                                        tCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:5309093/150‑1 (MQ=39)
                                                                                                                        tCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:7529259/150‑1 (MQ=39)
                                                                                                                        tCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:10021091/150‑1 (MQ=39)
                                                                                                                          atatAATCAGGGGCGCACGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt                             <  1:3790729/150‑1 (MQ=255)
                                                                                                                          atatAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCACACCCCCCCGCCCCCAGCCCCCCCCCGCTCTGGCGCACAAAATAGCCGCGGCGCCGGGCCCGCGGCGTATCCCGTAACGCACCCGGGGCGGGCGCGGGGGAACaaaccggg                             >  2:8369536/1‑143 (MQ=2)
                                                                                                                               aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAc                        <  1:9164564/150‑1 (MQ=33)
                                                                                                                               aTCACGGGCGCAAGGTGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAc                        <  1:4850052/150‑1 (MQ=16)
                                                                                                                                  aCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                     <  2:9153253/150‑1 (MQ=32)
                                                                                                                                      gcgcAAGGAGCGCGCAGGGGGCGGCCCAACGCCGCCGCCCCCAGCAACCCCCGGCACAGGCCAAAAAAATAGCCGCCGCGCGGTGGCCACGGCTTAACCCCTACCGCGACCCGGGCGGGCGCGCGGGAACAACCCAGTAAAACGCccccg                 >  2:721632/1‑149 (MQ=2)
                                                                                                                                          aaGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc             <  2:5677393/150‑1 (MQ=32)
                                                                                                                                           aGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc            <  2:7917639/150‑1 (MQ=32)
                                                                                                                                             gAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCGGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca          <  1:7555964/150‑1 (MQ=17)
                                                                                                                                                  cgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc     <  2:3829357/150‑1 (MQ=32)
                                                                                                                                                    cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt   <  1:6748947/150‑1 (MQ=32)
                                                                                                                                                    cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt   <  1:7323999/150‑1 (MQ=32)
                                                                                                                                                    cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt   <  2:6594681/150‑1 (MQ=32)
                                                                                                                                                     aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg  >  2:7283505/1‑150 (MQ=32)
                                                                                                                                                     aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg  <  2:765168/150‑1 (MQ=32)
                                                                                                                                                     |                                                                                                                                                      
TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: