Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,680 T→C 6.2% intergenic (+287/+428) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,6800TC 6.2% 836.9 / 25.6 308intergenic (+287/+428)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (105/175);  new base C (2/17);  total (111/197)
Fisher's exact test for biased strand distribution p-value = 2.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

TAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGC  >  NC_000913/2304531‑2304829
                                                                                                                                                     |                                                                                                                                                     
tAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                                                                                                                                       <  2:2465691/150‑1 (MQ=255)
 aGGTCAGACAAGGCGCTCACGCCGCATCCGACATCCAACGCCCCACCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCCTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTa                                                                                                                                                      <  2:414396/150‑1 (MQ=11)
  ggTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTaa                                                                                                                                                     <  1:1973955/150‑1 (MQ=40)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   >  2:5791163/1‑150 (MQ=39)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   >  2:1171912/1‑150 (MQ=39)
      aGACAAGGCGGTCACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCTGGATCGTATTATCATGCCTAAACCGCTGTGAAGTGCTCCAACCCGTATGTAGGATAAGGCGGTTAAGCAGAATCCGACATCTAACGcc                                                                                                                                                 >  2:6340556/1‑150 (MQ=11)
        aCATGGCGGTCACGCCGAATCCGACATCCAACGCCCGAGCCGGTTGCCTGTTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTTGGTCGGATATGGCGTTTACGCCGAATCCTACATCTAACGCCCa                                                                                                                                               <  1:4585751/150‑1 (MQ=11)
        aCAAGGCGGTCACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCa                                                                                                                                               >  2:265426/1‑150 (MQ=14)
        aCAAGGCGGTAACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCa                                                                                                                                               >  1:839383/1‑150 (MQ=18)
         cAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                                                              <  1:4967262/150‑1 (MQ=35)
         aaaGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGTTGCGACGCTGGCGCGTATTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTTATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                                                              <  1:632888/149‑1 (MQ=11)
            ggCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGcc                                                                                                                                           >  2:3111302/1‑150 (MQ=34)
              cGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGGTGCCTGATGCGTCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCAGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                                         <  2:1565314/150‑1 (MQ=255)
               ggacACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGTTGCGAGGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGt                                                                                                                                        <  1:3466584/147‑1 (MQ=255)
               ggTCACGCCGCTTCCGACATCCAACGCCCGAGCCGGTTGCCTTATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGt                                                                                                                                        <  1:2327229/150‑1 (MQ=21)
               ggTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTTAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCTACATCTAACGCCCAAGCCGGt                                                                                                                                        <  1:7058212/150‑1 (MQ=21)
                 tCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTa                                                                                                                                                      >  2:7113463/1‑134 (MQ=33)
                 tCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                      <  1:9941772/150‑1 (MQ=33)
                  cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                     <  1:6658341/150‑1 (MQ=32)
                  cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                     <  2:5480907/150‑1 (MQ=32)
                  cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                     <  1:8125280/150‑1 (MQ=32)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  1:3105285/150‑1 (MQ=32)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  2:7345483/150‑1 (MQ=32)
                    cgccgcTTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCTCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                   <  1:8660651/150‑1 (MQ=12)
                    cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                   <  2:5259893/150‑1 (MQ=32)
                    cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTATTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                   <  1:9285461/150‑1 (MQ=17)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 >  2:9113983/1‑150 (MQ=32)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  1:7721801/150‑1 (MQ=32)
                       cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                <  1:8626599/150‑1 (MQ=32)
                       cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                <  2:4070180/150‑1 (MQ=32)
                          aTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCg                                                                                                                             >  2:9493455/1‑150 (MQ=32)
                             cGACATCCAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  1:4397631/143‑1 (MQ=17)
                                   ccAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcg                                                                                                                    <  1:6196368/150‑1 (MQ=32)
                                    catcGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   <  2:184908/147‑1 (MQ=17)
                                    cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   <  1:1428395/150‑1 (MQ=32)
                                     aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                  >  2:8739374/1‑150 (MQ=17)
                                     aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                  >  2:8847673/1‑150 (MQ=17)
                                       cGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGGCGCTGGCGCGTc                                                                                                                <  2:8713039/150‑1 (MQ=17)
                                       cGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                <  1:8433138/150‑1 (MQ=32)
                                                                               TCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                        <  2:6085355‑M2/150‑1 (MQ=255)
                                                                               TCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCagc                                                                                                        >  1:1067534‑M2/1‑117 (MQ=255)
                                                                               TCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                        <  2:3926447‑M2/150‑1 (MQ=255)
                                                                               TCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                        >  1:4075000‑M2/1‑150 (MQ=255)
                                                                               TCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                        >  2:4641457‑M2/1‑150 (MQ=255)
                                                                               TCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                        <  2:216613‑M2/150‑1 (MQ=255)
                                                                               TCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                        <  2:4945718‑M2/150‑1 (MQ=255)
                                                                               TCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCGAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                        <  2:8934928‑M2/150‑1 (MQ=255)
                                                                                 AGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt                                                                      >  2:4702194‑M2/1‑150 (MQ=255)
                                                                                 AGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt                                                                      <  1:1610805‑M2/150‑1 (MQ=255)
                                                                                 AGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt                                                                      >  2:826946‑M2/1‑150 (MQ=255)
                                                                                 AGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt                                                                      >  1:5289279‑M2/1‑150 (MQ=255)
                                                                                 AGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt                                                                      >  1:6069126‑M2/1‑150 (MQ=255)
                                                                                 AGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt                                                                      <  1:9656121‑M2/150‑1 (MQ=255)
                                                                                 AGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGt                                                                      >  2:2756756‑M2/1‑150 (MQ=255)
                                                                                  GGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGtt                                                                     <  2:5073678‑M2/150‑1 (MQ=255)
                                                                                  GGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGtt                                                                     <  2:5676244‑M2/150‑1 (MQ=255)
                                                                                  GGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGtt                                                                     <  1:5531858‑M2/150‑1 (MQ=255)
                                                                                  GGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGtt                                                                     <  2:6500191‑M2/150‑1 (MQ=255)
                                                                                   cCCTAAACAGCAGTGAAGTGCTCCACCCCGTAGGTCAGACAAGGCGGTAACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACAGCTGTGAAGTGCTCCACCCCGTAGGTTg                                                                    <  2:1817696‑M2/149‑1 (MQ=255)
                                                                                   GCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCTACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                           <  1:2714314‑M2/111‑1 (MQ=255)
                                                                                   GCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTg                                                                    <  2:4556276‑M2/150‑1 (MQ=255)
                                                                                   GCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTg                                                                    >  1:3780314‑M2/1‑150 (MQ=255)
                                                                                   GCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTg                                                                    <  1:5791163‑M2/150‑1 (MQ=255)
                                                                                   GCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTg                                                                    <  2:5925519‑M2/150‑1 (MQ=255)
                                                                                   GCCTACACCGCTGTGAAGTGCTCCACCACGTAGGTCGGATAAGGCGGTTACGCAGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTTTCAGGCCTACTCCGCTGTGAAGTGCTCCACCCCGGAGGTTg                                                                    >  1:1294537‑M2/1‑150 (MQ=255)
                                                                                    CCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGccc                                                                                                                                                <  2:8038215‑M2/73‑1 (MQ=255)
                                                                                    CCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTgg                                                                   >  2:4776903‑M2/1‑150 (MQ=255)
                                                                                                           aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCg                                            <  2:110382/150‑1 (MQ=11)
                                                                                                           aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCg                                            >  2:9589186/1‑150 (MQ=11)
                                                                                                            ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                           <  1:7469453/150‑1 (MQ=31)
                                                                                                            ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                           >  2:7689110/1‑150 (MQ=31)
                                                                                                              ccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACa                                         >  2:9406787/1‑150 (MQ=32)
                                                                                                                    gTCGGATAAGGCGGTTACGCCGCATCCGACATCTACCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                   <  1:4623525/150‑1 (MQ=21)
                                                                                                                     tCGGATAAGGCGGTTACGCCGCATCCGACGTCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGACTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGGTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg                                  >  1:7180032/1‑150 (MQ=255)
                                                                                                                      cGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGGTGGATAATGCGGTTACGCCGCATCCGACATcgcacgc                                 >  2:3750999/1‑144 (MQ=255)
                                                                                                                             ggCGGTAACGCCGCATCCGACATCTAACGCCCAAGCGGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGcc                          <  1:7257638/150‑1 (MQ=255)
                                                                                                                              gCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCTACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                         <  1:4815671/150‑1 (MQ=255)
                                                                                                                              gCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                         <  1:5882305/150‑1 (MQ=37)
                                                                                                                              gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                         <  2:8002467/150‑1 (MQ=37)
                                                                                                                              gCGGTCACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                         <  2:7168331/150‑1 (MQ=255)
                                                                                                                                ggTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGt                       >  2:4335706/1‑150 (MQ=37)
                                                                                                                                   tACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                    <  1:10014834/150‑1 (MQ=37)
                                                                                                                                     cgccgcATCCGACATCTCACGCCCCAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                  <  2:2520593/150‑1 (MQ=255)
                                                                                                                                     cgccgcATCCGACATCTAACGCCCTAGCGGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                  <  2:9832372/150‑1 (MQ=255)
                                                                                                                                     cgccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                  <  2:6636684/150‑1 (MQ=37)
                                                                                                                                     cgccgcAGCCGACATCCCCCGCCCGCGCCGGGTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGATAAGGCGGGTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                  <  2:9594263/150‑1 (MQ=11)
                                                                                                                                      gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGGTGCCTg                 >  1:4895524/1‑150 (MQ=255)
                                                                                                                                      gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTg                 >  2:6740673/1‑150 (MQ=21)
                                                                                                                                       ccgcGTCCGACATCTAACGCCCAAGCCGGTTGCCTGATCCGACGCTGGCGCGTCTTATCAGGACTACACCGCTGAGAAGTGCTCCACCCCGTAGGTTGGATCAGGCGGTTACGCCGAATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                >  1:9064127/1‑150 (MQ=11)
                                                                                                                                       ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                <  1:8010263/150‑1 (MQ=37)
                                                                                                                                       ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                <  2:3447248/150‑1 (MQ=21)
                                                                                                                                        cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt               >  1:9466673/1‑150 (MQ=37)
                                                                                                                                         gcttCCTACATCTCTCGCCCGAGCCGTTTGCGTTATTCTACTCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTTGGAAAAGGCGGTAACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATg              <  1:2511119/147‑1 (MQ=11)
                                                                                                                                              cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACg         >  2:7944611/1‑150 (MQ=37)
                                                                                                                                               gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGc        >  2:5979877/1‑150 (MQ=21)
                                                                                                                                                aCATCTTTCGCCCAAGCCGGTTGCCTGTTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGTCGCt       <  1:5781459/150‑1 (MQ=255)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc  >  1:2142559/1‑150 (MQ=37)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc  >  1:8897059/1‑150 (MQ=37)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCAGCCGACCTCCACCGGCCGCGCCGGTTGCCTGATGCGACGCTGgcgc  >  1:6875775/1‑150 (MQ=11)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCGCCACCCCGTAGGGTGGATAAGGCGGTTACGCCGCAGCCGACAACCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc  >  1:5108470/1‑150 (MQ=11)
                                                                                                                                                     |                                                                                                                                                     
TAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGC  >  NC_000913/2304531‑2304829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: