Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,383,959 A→T 6.7% intergenic (+35/+36) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,383,9590AT 6.7% 1365.1 / 61.9 398intergenic (+35/+36)elaD/yfbKprotease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (174/195);  new base T (13/14);  total (187/211)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 416 total aligned reads displayed.

CAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATCTGCTG  >  NC_000913/2383810‑2384108
                                                                                                                                                     |                                                                                                                                                     
cAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAata                                                                                                                                                       >  2:1541185/1‑150 (MQ=255)
 aGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCACAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCACatat                                                                                                                                                      >  2:9684966/1‑150 (MQ=255)
 aGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAatat                                                                                                                                                      <  1:155254/150‑1 (MQ=255)
 aGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAatat                                                                                                                                                      >  2:1169083/1‑150 (MQ=255)
 aGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAatat                                                                                                                                                      <  2:8278109/150‑1 (MQ=255)
   aaaTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGc                                                                                                                                                    <  1:4197885/150‑1 (MQ=255)
   aaaTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGc                                                                                                                                                    <  1:5402940/150‑1 (MQ=255)
      ttGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGa                                                                                                                                                 <  2:5659358/150‑1 (MQ=255)
        gTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACt                                                                                                                                               >  1:4223885/1‑150 (MQ=255)
        gTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACt                                                                                                                                               >  2:173598/1‑150 (MQ=255)
        gTTATTTAGAGAAATATGGCGATGCAAATATCAATCATTATTATAGAACCTTAGAACTAAAACACTCACCACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACt                                                                                                                                               <  2:1319863/150‑1 (MQ=255)
         ttATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACTg                                                                                                                                              <  2:9634661/150‑1 (MQ=255)
         ttATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTg                                                                                                                                              >  1:8480904/1‑150 (MQ=255)
           aTTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACTGGt                                                                                                                                            <  1:6619654/150‑1 (MQ=255)
            tttAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTc                                                                                                                                           >  2:9043117/1‑150 (MQ=255)
               aGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAACAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGaa                                                                                                                                        <  2:2107894/150‑1 (MQ=255)
               aGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGaa                                                                                                                                        <  2:6139258/150‑1 (MQ=255)
                   aaaTATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCACATATGCTGACTGGTCGAACATa                                                                                                                                    >  1:6301215/1‑150 (MQ=255)
                   aaaTATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATa                                                                                                                                    >  2:5172310/1‑150 (MQ=255)
                       atGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGttt                                                                                                                                >  1:6959467/1‑150 (MQ=255)
                       atGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGttt                                                                                                                                >  1:3590004/1‑150 (MQ=255)
                        tGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTTATTTAACATCATTGTTAATTAATTCGACCAGTCAGAAATTATGCTGACTGGTCGAACATAGTTTg                                                                                                                               <  1:4825131/150‑1 (MQ=255)
                           cGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTa                                                                                                                            >  1:4049911/1‑150 (MQ=255)
                           cGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTa                                                                                                                            >  2:8582269/1‑150 (MQ=255)
                             aTGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTaaa                                                                                                                          >  2:7403471/1‑150 (MQ=255)
                               gCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACa                                                                                                                        <  2:5525978/150‑1 (MQ=255)
                                cAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAg                                                                                                                       >  1:8285845/1‑150 (MQ=255)
                                 aaaTATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAAAATGCTGACTGGTCGAACATAGTTTGCTAAACAGt                                                                                                                      >  1:8761664/1‑150 (MQ=255)
                                 aaaTATAAATAATTATTATAGAAACTTACAAATAAAACACTCACAACCAAAAAATAGAGCACCCGGCAAACGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGt                                                                                                                      <  1:3477528/150‑1 (MQ=255)
                                  aaTATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGtc                                                                                                                     <  2:9526813/150‑1 (MQ=255)
                                     atcaatcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGtcatc                                                                                                                  >  2:6065656/1‑150 (MQ=255)
                                      tcaatcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGtcatca                                                                                                                 <  2:2997095/150‑1 (MQ=255)
                                         atcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactg                                                                                                              >  1:5697220/1‑150 (MQ=255)
                                          tcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactga                                                                                                             <  2:6374509/150‑1 (MQ=255)
                                          tcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactga                                                                                                             <  1:5111795/150‑1 (MQ=255)
                                               attatAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGAt                                                                                                        <  2:4958520/150‑1 (MQ=255)
                                               attatAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGAt                                                                                                        <  2:3376084/150‑1 (MQ=255)
                                                 tataGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCTACCAGTCAGCAAATATGCTGAATGGTCGAACATATTTTGCTAAACAGTAATCACTGACTGATgt                                                                                                      >  1:5306272/1‑150 (MQ=255)
                                                   tataaaCTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtca                                                                                                    <  1:8463772/147‑1 (MQ=255)
                                                   taGAAACTTAGAAATAAAACACTCACCACCCAAAAAGAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtca                                                                                                    <  2:7088396/150‑1 (MQ=255)
                                                   taGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCCTCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtca                                                                                                    <  1:6417029/150‑1 (MQ=255)
                                                   taGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCAGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtca                                                                                                    <  1:2331817/150‑1 (MQ=255)
                                                     gAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAATTATGCTGACTGGTCGAACATAGTTTGCTATACAGTCATCACTGACTGTTgtcagt                                                                                                  >  2:7283706/1‑150 (MQ=255)
                                                       aaCTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagtca                                                                                                <  2:9116976/150‑1 (MQ=255)
                                                       aaCTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagtca                                                                                                <  1:8378058/150‑1 (MQ=255)
                                                        aCTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTcac                                                                                               <  1:4295931/150‑1 (MQ=255)
                                                         cTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTcaca                                                                                              <  2:5930204/150‑1 (MQ=255)
                                                         cTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGACatcagtcagt                                                                                              <  1:6742192/150‑3 (MQ=255)
                                                          aaacaaaaaaaaCACACACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCacac                                                                                             >  1:9919799/5‑150 (MQ=255)
                                                             gAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCAt                                                                                          >  1:1174527/1‑150 (MQ=255)
                                                              aaaTAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATc                                                                                         >  1:2588219/1‑150 (MQ=255)
                                                                 tAAAACACTCACAACCCAAAAATAGAGCATCTGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGc                                                                                      >  1:2776663/1‑150 (MQ=255)
                                                                 tAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATAGGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGc                                                                                      <  1:634406/150‑1 (MQ=255)
                                                                    aaCACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAg                                                                                   >  1:2897606/1‑150 (MQ=255)
                                                                    aaCACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAg                                                                                   >  1:4388767/1‑150 (MQ=255)
                                                                       acTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTc                                                                                <  2:4042791/150‑1 (MQ=255)
                                                                           acaACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAAATCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATc                                                                            >  2:588080/1‑150 (MQ=255)
                                                                                ccAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCg                                                                       >  1:3241564/1‑150 (MQ=255)
                                                                                 cAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGa                                                                      >  1:8580139/1‑150 (MQ=255)
                                                                                       tAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAAt                                                                <  1:4639286/150‑1 (MQ=255)
                                                                                          agCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTcc                                                             <  1:7759527/150‑1 (MQ=255)
                                                                                            cATCCGTCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGc                                                           <  2:5357086/150‑1 (MQ=255)
                                                                                            cATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGc                                                           <  1:7490070/150‑1 (MQ=255)
                                                                                             tatgggCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCTAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGcc                                                          >  2:346931/5‑150 (MQ=255)
                                                                                             aTCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGcc                                                          >  1:1037136/1‑150 (MQ=255)
                                                                                               ccGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCt                                                        <  2:7741732/150‑1 (MQ=255)
                                                                                                 ggCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGt                                                      >  1:1883764/1‑150 (MQ=255)
                                                                                                   cAAAAGAGTGATTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTaa                                                    <  1:3029554/150‑1 (MQ=255)
                                                                                                         agtgagtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCtgt                                              <  1:1273801/150‑1 (MQ=255)
                                                                                                           tgagtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTgg                                            <  2:1904250/150‑1 (MQ=255)
                                                                                                             agtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGAt                                          <  1:9447983/150‑1 (MQ=255)
                                                                                                             agtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGAGAATAAATTCCGCCCTGTAACCCTGTGGAt                                          <  2:4409750/150‑1 (MQ=255)
                                                                                                                   catcatTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTccc                                    <  2:809694/150‑1 (MQ=255)
                                                                                                                    atcatTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTcccc                                   >  2:4109109/1‑150 (MQ=255)
                                                                                                                     tcatTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCt                                  >  1:5931628/1‑150 (MQ=255)
                                                                                                                       atTGTTAATTAAGTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCttt                                <  1:8965253/150‑1 (MQ=255)
                                                                                                                        ttGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCtttt                               <  2:3590176/150‑1 (MQ=255)
                                                                                                                         tGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTg                              <  1:9670001/150‑1 (MQ=255)
                                                                                                                          gTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGc                             >  1:7318383/1‑150 (MQ=255)
                                                                                                                              attaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACAGACTGATGTCAGTCACACCATACGCCAGTTCAATCAGGCGAATAAATTGCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctgc                         >  1:9249210/1‑150 (MQ=255)
                                                                                                                               ttaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctgct                        >  2:2512247/1‑150 (MQ=255)
                                                                                                                                  attCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATAACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAg                     >  1:5794027/1‑150 (MQ=255)
                                                                                                                                       aCCAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCAc                >  1:7122788/1‑150 (MQ=255)
                                                                                                                                        ccAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACt               >  2:2830430/1‑150 (MQ=255)
                                                                                                                                         cAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTg              >  1:3335763/1‑150 (MQ=255)
                                                                                                                                         cAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTg              >  1:6113567/1‑150 (MQ=255)
                                                                                                                                         cAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTg              <  2:3205194/150‑1 (MQ=255)
                                                                                                                                         cAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCACCTTTTGCCTGCTGAGCCCACTg              >  1:1965729/1‑150 (MQ=255)
                                                                                                                                           gTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGtt            >  1:3402853/1‑150 (MQ=255)
                                                                                                                                            tCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGttt           >  2:799062/1‑150 (MQ=255)
                                                                                                                                            tCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGttt           >  1:3621172/1‑150 (MQ=255)
                                                                                                                                            tCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGttt           >  1:180884/1‑150 (MQ=255)
                                                                                                                                              aGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGa         >  1:7941592/1‑150 (MQ=255)
                                                                                                                                               gCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAt        <  2:8431524/150‑1 (MQ=255)
                                                                                                                                               gCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAt        >  1:2459821/1‑150 (MQ=255)
                                                                                                                                               gCAAATAGGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAt        <  2:9364620/150‑1 (MQ=255)
                                                                                                                                                 aaaTATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCCGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCATGCTGAGCCCACTGTTTTATct      >  2:6480258/1‑150 (MQ=255)
                                                                                                                                                   atatGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATctgc    <  2:7552748/150‑1 (MQ=255)
                                                                                                                                                   atatGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATctgc    <  1:9043117/150‑1 (MQ=255)
                                                                                                                                                     atGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATctgctg  <  2:6682829/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATCTGCTG  >  NC_000913/2383810‑2384108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: