Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,431 G→T 8.3% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4310GT 8.3% 1180.1 / 8.4 405R434I (AGA→ATA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (147/208);  new base T (29/6);  total (190/215)
Fisher's exact test for biased strand distribution p-value = 3.16e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 410 total aligned reads displayed.

GTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470283‑2470580
                                                                                                                                                    |                                                                                                                                                     
 taTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGaa                                                                                                                                                     <  1:957327/150‑1 (MQ=255)
  aTCTCTTTTTGTAATATCTCAATTTTATGTGTCATTTTTCGGGGAATGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTATACCTTTGCTTATACATATCAGTAGTTTTTTTAATTTATAAAATAATTCAAAGaaa                                                                                                                                                    <  1:346372/150‑1 (MQ=255)
  aTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGaaa                                                                                                                                                    <  2:4314697/150‑1 (MQ=255)
   tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                                                                                                   >  2:6642127/1‑150 (MQ=255)
     tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACACAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                                                                                                 <  1:6686578/150‑1 (MQ=255)
       tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAACaaa                                                                                                                                               >  2:5267218/1‑149 (MQ=255)
       tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTATTTTAATTTATAAAATAATTAAAAGAAATaaaa                                                                                                                                               >  1:6005370/1‑149 (MQ=255)
         tttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAc                                                                                                                                             <  1:7148627/150‑1 (MQ=255)
         tttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAATAAATAc                                                                                                                                             >  2:7229423/1‑150 (MQ=255)
                  tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTACTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                    <  1:2105225/150‑1 (MQ=255)
                  actcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGACATTTAAGCAAGAATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                    <  1:2623405/149‑1 (MQ=255)
                   ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGAAAATAGCGATg                                                                                                                                   >  1:5797164/1‑150 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                  <  1:6519770/150‑1 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAATAGCGATGt                                                                                                                                  >  2:6208318/1‑150 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAAAAAAAAAAAAAGCGAAGt                                                                                                                                  >  1:9460159/1‑150 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTGTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAAATCAAAGAAAAAAAGACAAAAGCAATGt                                                                                                                                  >  2:9352787/1‑150 (MQ=255)
                      aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAAACAAAAGAGAtttta                                                                                                                                >  1:944322/1‑146 (MQ=255)
                        ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                              <  1:7883071/150‑1 (MQ=255)
                          ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                            <  1:6479751/150‑1 (MQ=255)
                             tgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTAAACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                                                                                                         <  2:1549775/150‑1 (MQ=255)
                                gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                                                                                                      >  2:4095523/1‑150 (MQ=255)
                                gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAATAAAGAAAAAAGAGATTTAAAGCACaaatt                                                                                                                      >  2:3429824/1‑148 (MQ=255)
                                 tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAAACAAGAAAAAAGCGATGTTAAGCCCTAAGtt                                                                                                                     >  1:5124803/1‑150 (MQ=255)
                                  cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                    <  1:2338884/150‑1 (MQ=255)
                                   aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATATCGAGGTAAAGCACAAAGTTTa                                                                                                                   >  1:6994503/1‑150 (MQ=255)
                                   aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAAAAAAATATCGATGTAAATCACAAAGTTTa                                                                                                                   >  1:6791257/1‑150 (MQ=255)
                                    tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAACAAAATAGAGATGTAAAGCACAAAGTTTaa                                                                                                                  >  1:1299972/1‑150 (MQ=255)
                                     ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                 <  2:1400039/150‑1 (MQ=255)
                                          ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                            <  2:6058023/150‑1 (MQ=255)
                                             gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAATCAAGAAAATAGCGATTTAAAGCACTAAGGTTAAAATtcgcgc                                                                                                         >  2:7373220/1‑150 (MQ=255)
                                              aaGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAATCGATGTAAAGCACTAAGTTTAAATTTCGCGcc                                                                                                        >  2:3117208/1‑150 (MQ=255)
                                                    ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATAAAAAAGCCATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                                                                                                  >  2:3821227/1‑150 (MQ=255)
                                                     taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                 <  1:3807783/150‑1 (MQ=255)
                                                     taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                 <  2:4891495/150‑1 (MQ=255)
                                                     taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTTTTTTTAAAATAATTAAAAGAAAAAAAGAAAAAAACGATGGAAATCACGAAGTTTAAAATTCGCGCCCCTCATg                                                                                                 >  1:4588464/1‑150 (MQ=255)
                                                     taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGATTATACATAACAGTCGTTTTTTTTATTTATAAAAAAATTCAAATAAATAAAGACAAAAGCCGTGTAAAGCACTAAGTTTAAATTTCGGGcccaacaag                                                                                                 >  2:4556361/1‑143 (MQ=255)
                                                      agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAACAAAAAATCGAAGTAAATCACAAAGTTTAAATTTAGCGCCaaaaaagg                                                                                                >  2:2945001/1‑144 (MQ=255)
                                                        agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAAGAAATAAAAACAAAAGCGATGTTAATAACGAAGTTTTAATTTGGGGCCAAACATgggg                                                                                              >  1:7338499/1‑148 (MQ=255)
                                                         gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                             <  2:8120791/150‑1 (MQ=255)
                                                             tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGAACTAAGTTTAAATTTCGCGCCCAGCATGGCGCGcac                                                                                         >  1:5459609/1‑150 (MQ=255)
                                                              aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaat                                                                                        <  2:6302998/150‑3 (MQ=255)
                                                               ataatgcaTTTATTTGGATTTTATTTTTCGTTCGACCTTTGCTTATAAATAACAGTCTTTTTTTTATTTTATAAAATAATTAAAAGAAATCAAGACAATAGCGTTGTAAAGCACTAATTTTATATTGCGCGCCAATCATGGCGCGCACaa                                                                                       <  1:6229246/145‑1 (MQ=255)
                                                               agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                       <  2:1819666/150‑1 (MQ=255)
                                                                 caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAAGAAATAAAGACAAAAGAGATGTAAATCACTAAGTTTAAAATGAGCGCCAAACATGGCGCGCACAAGc                                                                                     >  2:2113609/1‑150 (MQ=255)
                                                                  aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAAAAAAGACAAAAGCGATGTAAAGCACAAAGTTTAAATTGCGCGCCAATCAAGGCGCGCACAAGCt                                                                                    >  2:3627892/1‑150 (MQ=255)
                                                                  aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATAAAGACAAAAGAGATGTAAAGCACAAAGTTTAAATTTCGCGCCAATCAAGGCGCGCACAAGcg                                                                                    >  2:267731/1‑149 (MQ=255)
                                                                     caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAATAAAAAAAGAAAAAAGCGAATTAAAGAACTAAGTTTAAAATTAGCGCCAATAAATGCGCGCACAAgagata                                                                                 >  2:1016702/1‑145 (MQ=255)
                                                                     caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTTTAAAAAAAATAAAATAAATAAAAAAAAAATAGATGTAAAGAAAGAAGTTTAAAATTCGCGCCAAACAAGGGGCGCACCAACtata                                                                                 >  1:9913696/1‑150 (MQ=255)
                                                                         tatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAc                                                                             <  2:3250840/150‑1 (MQ=255)
                                                                          atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                            <  2:4760109/150‑1 (MQ=255)
                                                                          atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                            <  2:9642588/150‑1 (MQ=255)
                                                                          atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAAAAATAAAGACAAAAGGGTTGTTAATAAATAATTTTTAAATTAGCGCCAAAAAAGGGGCGCCCAAGCAaaaataaa                                                                            >  1:2243359/1‑148 (MQ=255)
                                                                           tttGGAATTTATTTTTCGTTCGACCTTTTATTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACATTTGCTATGTAAAGCAATAAGTTTAATTTGCGCGCAAATCTTGGCGCGCACAAGCTATAATACCa                                                                           <  1:1192924/150‑1 (MQ=255)
                                                                           tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGAAAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATAAATGCGCGCACAAGCTataataaaa                                                                           >  2:8792843/1‑147 (MQ=255)
                                                                                     aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                                 <  2:5186718/150‑1 (MQ=255)
                                                                                      tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATACAATAGCGATTTAAAGCACTAAGTTTAAATTGAGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCGAAATtct                                                                >  2:2291029/1‑150 (MQ=255)
                                                                                       ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                               <  2:5781064/150‑1 (MQ=255)
                                                                                           cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                           <  1:5160607/150‑1 (MQ=255)
                                                                                                gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGAACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTa                                                      <  1:10026911/150‑1 (MQ=255)
                                                                                                       gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                               <  2:4643190/150‑1 (MQ=255)
                                                                                                         ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACCAGCTCTAATACCAACCTAATTTCTCCTCCTCGTAGAGTGACta                                             >  2:9090889/1‑150 (MQ=255)
                                                                                                         ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                             <  1:18365/150‑1 (MQ=255)
                                                                                                         ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                             <  1:8556106/150‑1 (MQ=255)
                                                                                                         ttATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAATAAATAAAAAAAAAAGCGATGTAAATGACTAAGTTTAAATTGAGCGCCAAAAATTGCGCGCACAAGCTATAAAAACAAACAAAATTGAACTCCTCTTAGAGTGACta                                             >  2:8540692/1‑150 (MQ=255)
                                                                                                          tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCTATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                            <  2:7967112/150‑1 (MQ=255)
                                                                                                               aTAACCGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                       <  1:7723781/150‑1 (MQ=255)
                                                                                                                tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                      <  1:841498/150‑1 (MQ=255)
                                                                                                                   cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                   <  1:8216175/150‑1 (MQ=255)
                                                                                                                    tttcttttttttAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCTATGTAAAGCACTTTGTTTAAATTTCGCGCCACTCATGTCGCGCACAAGCTATAATACCATCCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGAt                                  <  1:8959889/145‑1 (MQ=255)
                                                                                                                    aGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGAt                                  <  2:3694713/150‑1 (MQ=255)
                                                                                                                     gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                 <  2:2508420/150‑1 (MQ=255)
                                                                                                                        gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                              <  2:1216448/150‑1 (MQ=255)
                                                                                                                        gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGAGACTATATCTCCTGATAGaa                              >  2:5426178/1‑150 (MQ=255)
                                                                                                                         tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                             >  2:3976867/1‑150 (MQ=255)
                                                                                                                         tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                             >  1:1698875/1‑150 (MQ=255)
                                                                                                                         tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                             >  1:3027909/1‑150 (MQ=255)
                                                                                                                          ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                            >  1:3347204/1‑150 (MQ=255)
                                                                                                                             tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTGTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATCGAATTGCg                         >  2:7026946/1‑150 (MQ=255)
                                                                                                                              ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                        >  1:8328811/1‑150 (MQ=255)
                                                                                                                               tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGTGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                       >  2:5007650/1‑150 (MQ=255)
                                                                                                                               tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                       <  1:1911146/150‑1 (MQ=255)
                                                                                                                                  tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                    >  1:5587439/1‑150 (MQ=255)
                                                                                                                                    tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  1:9233934/1‑150 (MQ=255)
                                                                                                                                    tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  2:2343991/1‑150 (MQ=255)
                                                                                                                                     ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                 <  2:6152501/150‑1 (MQ=255)
                                                                                                                                     ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                 <  2:7505993/150‑1 (MQ=255)
                                                                                                                                     ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                 >  1:8780341/1‑150 (MQ=255)
                                                                                                                                        aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt              >  1:8846633/1‑150 (MQ=255)
                                                                                                                                        aaaTAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt              <  2:6863268/150‑1 (MQ=255)
                                                                                                                                         aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTATATTGCGCGCCAATCATGGCGCGCACAAGCTTTAATACCAACCTTATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             <  2:8198180/150‑1 (MQ=255)
                                                                                                                                         aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             <  2:5515481/150‑1 (MQ=255)
                                                                                                                                          ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa            <  1:7979962/150‑1 (MQ=255)
                                                                                                                                           taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa           <  1:4420317/150‑1 (MQ=255)
                                                                                                                                            aatTCAAAGAAATCAAGCAAATAGCTATGTAAAGCTCTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAACTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa          <  1:3748690/150‑1 (MQ=255)
                                                                                                                                            aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa          <  2:3371988/150‑1 (MQ=255)
                                                                                                                                               tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc       <  2:7654607/150‑1 (MQ=255)
                                                                                                                                                 aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGtcc     >  2:8325000/1‑150 (MQ=255)
                                                                                                                                                 aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  1:3906467/1‑150 (MQ=255)
                                                                                                                                                 aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  1:4869385/1‑150 (MQ=255)
                                                                                                                                                    gAAATCAAGACAATAGCGATTTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGAGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTATAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa  <  1:4799633/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470283‑2470580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: