Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,436 C→A 16.0% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4360CA16.0% 1142.3 / 66.7 401Q436K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (120/206);  new base A (70/5);  total (190/211)
Fisher's exact test for biased strand distribution p-value = 1.29e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 409 total aligned reads displayed.

TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585
                                                                                                                                                    |                                                                                                                                                     
 ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                     <  2:4820440/150‑1 (MQ=255)
 ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                     <  1:4997479/150‑1 (MQ=255)
  tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAAATAAAAAAAAACaaa                                                                                                                                                    >  1:6005354/1‑149 (MQ=255)
    tttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAATAAATAc                                                                                                                                                  >  2:7229423/1‑150 (MQ=255)
       ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACaaa                                                                                                                                               >  1:5731547/1‑149 (MQ=255)
             tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTACTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  1:2105225/150‑1 (MQ=255)
             actcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGACATTTAAGCAAGAATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  1:2623405/149‑1 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                       <  1:6519770/150‑1 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAAAAAGAAAATAGAGAttt                                                                                                                                       >  2:6208296/1‑148 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTTTAAAATAATTGAAAGAAAACAATACAAAAGCGATGt                                                                                                                                       >  1:9459498/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTGTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAAATCAAAGAAAAAAAGACAAAAGCAATGt                                                                                                                                       >  2:9352787/1‑150 (MQ=255)
                 aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATATCGATGTaa                                                                                                                                     >  2:3288836/1‑150 (MQ=255)
                  aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                                    >  1:4818057/1‑150 (MQ=255)
                   ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                   <  1:7883071/150‑1 (MQ=255)
                        tgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTAAACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                                                                                                              <  2:1549775/150‑1 (MQ=255)
                            tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                          <  1:9142892/150‑1 (MQ=255)
                            tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAAAAAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                          >  1:5124877/1‑150 (MQ=255)
                            tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACATTTGATTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAAAAATAAACAAAAAATAGATGTAAATCGCTaatgt                                                                                                                          >  1:7076132/1‑147 (MQ=255)
                             cATTTTTCGGTGATGGATATAGAGATTTAAGCAAGCATTTATTTGGATTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCATAGAAATCAAGACAATAGCGATGTAAAGCTCTAAGttt                                                                                                                         <  1:963355/150‑1 (MQ=255)
                             cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                         <  1:2338884/150‑1 (MQ=255)
                             cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAAAAATACAATAGAGATTTAAATCACTAAGttt                                                                                                                         >  1:6708459/1‑150 (MQ=255)
                              aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCAATAAGTTTa                                                                                                                        >  2:7517395/1‑150 (MQ=255)
                               tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTGTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                       <  2:178829/150‑1 (MQ=255)
                               tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAACAAAATAGAGATGTAAAGCACAAAGTTTaa                                                                                                                       >  1:1299972/1‑150 (MQ=255)
                                ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                      <  1:9963425/150‑1 (MQ=255)
                                ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                      <  2:1400039/150‑1 (MQ=255)
                                   tCGGGGAAGGAAATAGATAATTAAGCAAGCATTTATTTGGAATGTATTTTACGTTCGACCTTTGCTTATACATAACAGTCGTTATATTAATATATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTg                                                                                                                   <  2:640894/150‑1 (MQ=255)
                                       ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                                                                                                               <  1:6637796/150‑1 (MQ=255)
                                        gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAATCAAGAAAATAGCGATTTAAAGCACTAAGGTTAAAATtcgcgc                                                                                                              >  2:7373220/1‑150 (MQ=255)
                                                taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                      <  1:3807783/150‑1 (MQ=255)
                                                 agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAACAAAAAATCGAAGTAAATCACAAAGTTTAAATTTAGCGCCaaaaaagg                                                                                                     >  2:2945001/1‑144 (MQ=255)
                                                  gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                    >  2:2339662/1‑150 (MQ=255)
                                                  gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAAAAAAAAAAAAAAAAGAGATGTAAAACACAAAGTTTTAATTTAGCGCCAAACAAGgc                                                                                                    >  2:4535848/1‑150 (MQ=255)
                                                   agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAAGAAATAAAAACAAAAGCGATGTTAATAACGAAGTTTTAATTTGGGGCCAAACATgggg                                                                                                   >  1:7338499/1‑148 (MQ=255)
                                                   agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTAATTTTAAAATAATTAAAAGAAATAAATACAATAGAGATTTAAAGAAATAAGTTTTAATTGAGGGCCCATCATGgcg                                                                                                   >  1:6895230/1‑150 (MQ=255)
                                                    gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                                  <  2:8120791/150‑1 (MQ=255)
                                                     aTTTAAGCAAGCAATTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACAGAACAGCCGTTTTTTTAATTTATACCATAATTCAAAGAAATCAAGACAATAGCGAGGTAACGCACTAAGTTTAACTTGCGCGCCCATCATGgcgcg                                                                                                 <  2:4732192/150‑1 (MQ=255)
                                                       ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAAAAAACAAGACAAAATCGATGTAAAGCACTAATTTTAAAATTCGCGCCACACATGgcgcgcc                                                                                               >  1:6279868/1‑149 (MQ=255)
                                                          agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaatt                                                                                            <  2:2629811/150‑4 (MQ=255)
                                                          agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTTTTAAATAATTAAAAAAAAAAAAAAAAAAATAGATTGAAATGACTAAATTTTAATTTCGCGCCAAAGATGTCGCGCCCaa                                                                                            >  1:2762411/1‑150 (MQ=255)
                                                             aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAAAAAAGACAAAATCGATGTAAAGCACGAAGTTTAAATTGCGCGCCCAAAATGGCGCGCACAAGCt                                                                                         >  2:6035028/1‑150 (MQ=255)
                                                               gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtat                                                                                       <  2:2146987/150‑1 (MQ=255)
                                                                caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAATAAAAAAAGACAATAGCGATGTAAATCAAAAATTTTAAATTTCGCCCCAAAAATTGCGCGCACaaacaaaa                                                                                      >  2:1017672/1‑144 (MQ=255)
                                                                 atttatttGGAATGTATTTTTCGTTCGACCTTTTCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                                     <  2:3818710/150‑1 (MQ=255)
                                                                     atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                                 <  2:9642588/150‑1 (MQ=255)
                                                                     atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAAGCCAAGTAAAGCACTAAGTTTAAATTTAGCGCCAAACAAGGCGCGCACAAGCGATAATAcc                                                                                 >  2:6132835/1‑150 (MQ=255)
                                                                     atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAAAAATAAAGACAAAAGGGTTGTTAATAAATAATTTTTAAATTAGCGCCAAAAAAGGGGCGCCCAAGCAaaaataaa                                                                                 >  1:2243359/1‑148 (MQ=255)
                                                                      tttGGAATTTATTTTTCGTTCGACCTTTTATTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACATTTGCTATGTAAAGCAATAAGTTTAATTTGCGCGCAAATCTTGGCGCGCACAAGCTATAATACCa                                                                                <  1:1192924/150‑1 (MQ=255)
                                                                      tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGAAAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATAAATGCGCGCACAAGCTataataaaa                                                                                >  2:8792843/1‑147 (MQ=255)
                                                                      tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAATCAAAACAAAAGCCAAGTAAAGCACTAAGTTTAAATTTCGCGCCAATCAAGGCGCGCACAAGCGAAAATAcac                                                                                >  2:5416638/1‑148 (MQ=255)
                                                                        tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATAAAAAAAATAAAAAAAAAAAAAAAAAAATCGATTGAAAAAACAAAGTTTAAAATTAGCGCCAAAAAATGCGCGCACAAGCGaaaataaaaaa                                                                              >  1:344598/1‑145 (MQ=255)
                                                                                  ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                    <  2:340261/150‑1 (MQ=255)
                                                                                  ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                    <  2:5781064/150‑1 (MQ=255)
                                                                                         tcgACCTTTGATTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGCGATGTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCctct                                                             <  1:3288836/150‑1 (MQ=255)
                                                                                           gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGAACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTa                                                           <  1:10026911/150‑1 (MQ=255)
                                                                                              cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTagag                                                        <  2:3683460/150‑1 (MQ=255)
                                                                                                  gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                    <  2:5247614/150‑1 (MQ=255)
                                                                                                   cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                                   <  2:4416601/150‑1 (MQ=255)
                                                                                                    ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACCAGCTCTAATACCAACCTAATTTCTCCTCCTCGTAGAGTGACta                                                  >  2:9090889/1‑150 (MQ=255)
                                                                                                    ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                                  <  1:18365/150‑1 (MQ=255)
                                                                                                    ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                                  <  2:1870090/150‑1 (MQ=255)
                                                                                                    ttATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAATAAATAAAAAAAAAAGCGATGTAAATGACTAAGTTTAAATTGAGCGCCAAAAATTGCGCGCACAAGCTATAAAAACAAACAAAATTGAACTCCTCTTAGAGTGACta                                                  >  2:8540692/1‑150 (MQ=255)
                                                                                                     tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCTATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                                 <  2:7967112/150‑1 (MQ=255)
                                                                                                       taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                               <  2:6708459/150‑1 (MQ=255)
                                                                                                          aTAACCGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                            <  1:7723781/150‑1 (MQ=255)
                                                                                                           tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                           <  2:4993876/150‑1 (MQ=255)
                                                                                                           tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                           <  1:841498/150‑1 (MQ=255)
                                                                                                              cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                        <  1:8216175/150‑1 (MQ=255)
                                                                                                                   gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                   <  2:1216448/150‑1 (MQ=255)
                                                                                                                    tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                  >  2:3514/1‑150 (MQ=255)
                                                                                                                    tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                  >  1:1698875/1‑150 (MQ=255)
                                                                                                                    tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                  >  2:3157/1‑150 (MQ=255)
                                                                                                                      tttttAATTAATAAAATAATTCAAAGAAATCAAGACAATATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCGCTTAGAGTGTCTATATGTCCTGATAGAATTg                                >  1:5505011/1‑150 (MQ=255)
                                                                                                                       ttttAATTTAGAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAAGTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                               <  1:8796508/150‑1 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  2:3117043/150‑1 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              >  1:9387920/1‑150 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTGTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATCGAATTGCg                              >  2:7026946/1‑150 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGATTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  2:2451533/150‑1 (MQ=255)
                                                                                                                         ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                             <  2:4111294/150‑1 (MQ=255)
                                                                                                                         ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                             >  1:8328811/1‑150 (MQ=255)
                                                                                                                          tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                            <  1:1911146/150‑1 (MQ=255)
                                                                                                                           aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                           >  2:4000654/1‑150 (MQ=255)
                                                                                                                            aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAGGGAAAGCACTAAGTTTCAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                          <  2:5057221/150‑1 (MQ=255)
                                                                                                                             tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                         <  2:4622743/150‑1 (MQ=255)
                                                                                                                                ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                      >  1:5264637/1‑150 (MQ=255)
                                                                                                                                  aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa                    <  1:8912252/150‑1 (MQ=255)
                                                                                                                                    aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTATATTGCGCGCCAATCATGGCGCGCACAAGCTTTAATACCAACCTTATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                  <  2:8198180/150‑1 (MQ=255)
                                                                                                                                    aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                  <  2:5515481/150‑1 (MQ=255)
                                                                                                                                     ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa                 <  1:7979962/150‑1 (MQ=255)
                                                                                                                                     ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa                 <  2:629281/150‑1 (MQ=255)
                                                                                                                                      taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa                <  1:4420317/150‑1 (MQ=255)
                                                                                                                                       aatTCAAAGAAATCAAGCAAATAGCTATGTAAAGCTCTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAACTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa               <  1:3748690/150‑1 (MQ=255)
                                                                                                                                          tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc            <  2:7654607/150‑1 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGtcc          >  2:8325000/1‑150 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc          >  1:3906467/1‑150 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTACCAAATGccc          <  1:4679737/150‑1 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         >  1:857739/1‑150 (MQ=255)
                                                                                                                                                aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt      <  1:6179695/150‑1 (MQ=255)
                                                                                                                                                   tCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCg   <  1:4895093/150‑1 (MQ=255)
                                                                                                                                                    cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt  <  1:6514023/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: