Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,481,820 C→A 10.1% M119I (ATG→ATT emrY ← putative multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,481,8200CA10.1% 1378.3 / 14.6 476M119I (ATG→ATTemrYputative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (229/180);  new base A (2/64);  total (231/245)
Fisher's exact test for biased strand distribution p-value = 3.23e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 481 total aligned reads displayed.

AAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGA  >  NC_000913/2481671‑2481968
                                                                                                                                                     |                                                                                                                                                    
aaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGcc                                                                                                                                                      <  2:2424058/150‑1 (MQ=255)
    tATCACAAATAGCCCCGCCCAATCTCGGCCCACATATGGGAGGGATAATCCGGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATATTTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTa                                                                                                                                                  <  1:1889816/150‑1 (MQ=255)
       cacaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAcc                                                                                                                                               <  2:2368562/150‑1 (MQ=255)
        acaAATATAACCTCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                                                                                              <  2:6661733/150‑1 (MQ=255)
        acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                                                                                              >  1:334141/1‑150 (MQ=255)
        acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                                                                                              <  2:6026826/150‑1 (MQ=255)
        acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                                                                                              >  1:8442353/1‑150 (MQ=255)
        acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                                                                                              >  1:6020559/1‑150 (MQ=255)
         caAATATAACCGCCCACTATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACTGTGGAATTAACGGCCCCGCCATTAAcccc                                                                                                                                             <  1:7167415/150‑1 (MQ=255)
          aaaTATACCCGCCCACTATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCt                                                                                                                                            <  1:2431943/150‑1 (MQ=255)
          aaaTATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCt                                                                                                                                            <  1:5491530/150‑1 (MQ=255)
           aaTATACCCGCCCAATATCGGCCAACATATCGGAGCGATAATAATGGTAATTGAACATAATGCATGAGCAAATGTTATTTTTTCTGTCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTg                                                                                                                                           <  1:5589480/150‑1 (MQ=255)
             tataACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGaa                                                                                                                                         <  2:4034706/150‑1 (MQ=255)
             tataACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGaa                                                                                                                                         <  1:8888935/150‑1 (MQ=255)
                aaCCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGa                                                                                                                                      <  2:9174288/150‑1 (MQ=255)
                aaCCGCCCAATATCGGCCAACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGAAATTGTTCTTTTTTCTGGCGTATATTTCCTTAATAATAAACTCTTTGACTGTGTAATTAACGGCCCCGCCATTAACCCCTGAACGa                                                                                                                                      <  1:4435835/150‑1 (MQ=255)
                 aCCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGAc                                                                                                                                     >  2:8329082/1‑150 (MQ=255)
                 aCCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGAc                                                                                                                                     >  1:280240/1‑150 (MQ=255)
                  ccGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGAct                                                                                                                                    >  1:8272327/1‑150 (MQ=255)
                  ccGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGAct                                                                                                                                    >  1:6323095/1‑150 (MQ=255)
                   cGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGActc                                                                                                                                   >  1:5110513/1‑150 (MQ=255)
                    gCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGActct                                                                                                                                  >  2:7068914/1‑150 (MQ=255)
                     cccAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTa                                                                                                                                 >  2:5309711/1‑150 (MQ=255)
                       cAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaa                                                                                                                               >  1:8642760/1‑150 (MQ=255)
                         atatCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaaaa                                                                                                                             >  2:2973208/1‑150 (MQ=255)
                               gCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCa                                                                                                                       >  1:2651713/1‑150 (MQ=255)
                                 ccACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGc                                                                                                                     >  2:7217983/1‑150 (MQ=255)
                                     atatCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACAt                                                                                                                 >  2:9471684/1‑150 (MQ=255)
                                       atCGGAGCTATAATCAGGTTCTTTGACAATATTGACAGAGAAATTTTTTTTTTTTCTGGCGGTTATTACATTAATAATAAACTCTGTGACTGTTTATTTAACGGCCCCGCCTTTAACCCCTGAACGACTCTAAAAAATATCAGCACATCa                                                                                                               <  1:5663500/150‑1 (MQ=255)
                                        tCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCaa                                                                                                              >  2:5257858/1‑150 (MQ=255)
                                          ggAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGa                                                                                                            >  1:9104037/1‑150 (MQ=255)
                                           gAGCTATAATCCCGGTCATTTACCATAATGCCATAGAAATTTTTTTTTTTTCTGGGGTATATTTCATTAATAATAAACTCTGTTACTGTTTAATTAACGGCCCCGCAATTAACCCCTCAACGACTCCAAAAAATATCAGCACATCAAGAt                                                                                                           <  1:7521767/150‑1 (MQ=255)
                                            tgataaaaTCACGGTCATTTACAATAATGCCAGATAAATTTTTCTTTTTTGTGGGTTATAATTCATTAATAATAAACTCTGTCACAGTGTAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGAtt                                                                                                          <  1:2206814/144‑1 (MQ=255)
                                             gctaTAATCCGGGTCATTGACAATAAGGCCAGATCAATTTTTCTTTTTTCTGGCGGATAATACCTTAATAATAACCTCTGTGACTGTGCAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTg                                                                                                         <  1:5363155/147‑1 (MQ=255)
                                             gctaTAATCACTGAAATTGACAATAATGCATGAGAAAATTTTTTTTTTTGTGGGGTATATTTCATTAATAATAAACTGTGTGACTTTGTATTTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTg                                                                                                         <  1:2721532/147‑1 (MQ=255)
                                                       cGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGc                                                                                               >  2:3559388/1‑150 (MQ=255)
                                                        gttaaTTGACAATAATGCCAGAGAAAATGTTCTTTTTTCTGGCGTATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAAGGGCCCCGCCTTTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCt                                                                                              <  2:6292674/146‑1 (MQ=255)
                                                            aTTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTacac                                                                                          >  1:1054255/1‑150 (MQ=255)
                                                             ttGACAATACTGCCTGAGAATTTTTTTTTTTTTCTGGCGTATATTTCATTATTAAAAAAGTCTGTGACAGTGTATTTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTAcaca                                                                                         <  1:6846581/150‑1 (MQ=255)
                                                              tGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACAt                                                                                        >  2:9627075/1‑150 (MQ=255)
                                                               gACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACAtt                                                                                       >  2:617659/1‑150 (MQ=255)
                                                                acaaTAATGCCAGAGCAATTGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACTGTGTAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTa                                                                                      <  2:2775188/147‑1 (MQ=255)
                                                                aacATAATCCCATAGCATATGTTCTTTTTACTGGCGTATAATTCATTAATAATAAACTCTGTGACATTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTa                                                                                      <  2:1498633/148‑1 (MQ=255)
                                                                aCCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTa                                                                                      >  1:3050799/1‑150 (MQ=255)
                                                                 ccATAATTCAAGAGCAAATTTTATTTTTTCTGGGGGATAATTCATTAATAATAAACTCTGTTACAGTGTAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTaa                                                                                     <  2:8692122/150‑1 (MQ=255)
                                                                  cATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAAt                                                                                    <  2:4170443/150‑1 (MQ=255)
                                                                  cATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAAt                                                                                    >  1:10026883/1‑150 (MQ=255)
                                                                     atccccAGAGAAATTGTTCTTTTTTCGGGCTTATAATACATTAATAATAACCGCTTTGACTGGGGAATTAACGGCCCCGCCATTACCCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACCCATTAATGaa                                                                                 <  2:8513733/146‑1 (MQ=255)
                                                                     aaTGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGaa                                                                                 <  2:4989986/150‑1 (MQ=255)
                                                                       tGCCAGATCAAATGTTCTTTTTTCTGGCGGATAATTCATTAATAATAAACGCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGa                                                                               <  1:8083345/150‑1 (MQ=255)
                                                                       tGCCAGAGAAAATTTTCTTTTTTCTGGCGTATAATTCCTTAATAATAAACTCGGTGACTGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGa                                                                               <  1:4897544/150‑1 (MQ=255)
                                                                        gCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGAc                                                                              >  2:4359460/1‑150 (MQ=255)
                                                                         ccATAGAAAATGTTCTTTTTTCTGGCTTATAATTCCTTAATAATAAACTCTGTGACAGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACa                                                                             <  2:4730463/150‑1 (MQ=255)
                                                                         ccATAGAAAATGTTCTTTTTTCTGGCGTATAATTCATTAATAATAAACTATGTGACTGTGTAATTAACGGCCCCGCATTTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACa                                                                             <  2:5786239/150‑1 (MQ=255)
                                                                          ctgagCAATTTTTATTTTTTCTGGCGGATATTTACTTAATAATAAACTCTGTTACTGTTTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAg                                                                            <  1:4855561/148‑1 (MQ=255)
                                                                          cAGAGAAATTGTTCTTTTTTGTGGCGGATAATTCCTTAATAATAAACTGTGTGACAGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAg                                                                            <  2:6909801/150‑1 (MQ=255)
                                                                           agagCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGc                                                                           >  1:8761251/1‑150 (MQ=255)
                                                                           agagCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGc                                                                           >  2:5626272/1‑150 (MQ=255)
                                                                           agagCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGc                                                                           >  1:6414269/1‑150 (MQ=255)
                                                                                   ggTTCTTTTTTGTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGTAATTACCGGCCCCGCAATTAACCCCCCAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaaaa                                                                   <  1:9481202/149‑1 (MQ=255)
                                                                                        ttttttCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGa                                                              >  1:137061/1‑150 (MQ=255)
                                                                                        ttttttCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGa                                                              >  2:7434824/1‑150 (MQ=255)
                                                                                               tGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAg                                                       >  2:7673386/1‑150 (MQ=255)
                                                                                                 gCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTa                                                     >  1:8712153/1‑150 (MQ=255)
                                                                                                 gCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTa                                                     >  2:4619934/1‑150 (MQ=255)
                                                                                                 gCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTa                                                     >  1:7398971/1‑150 (MQ=255)
                                                                                                        aTTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaataa                                              <  1:464409/150‑1 (MQ=255)
                                                                                                         ttCATTAATAATAAACTCTGTGACAGTGTAATTAACGGCCCCGCCTTTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaataat                                             <  1:4255346/150‑1 (MQ=255)
                                                                                                          tCCTTAATAATAAAATCGTTTACAGTGGAATTACCGGCCCCGAAATTACCCCCGTAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATc                                            <  2:8412777/150‑1 (MQ=255)
                                                                                                            cTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCtt                                          >  2:7916012/1‑150 (MQ=255)
                                                                                                            cTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCtt                                          >  1:5851112/1‑150 (MQ=255)
                                                                                                             ttAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTa                                         >  2:1660157/1‑150 (MQ=255)
                                                                                                                 taataaACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTc                                     >  2:227429/1‑150 (MQ=255)
                                                                                                                   ataaCCTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc                                   <  1:3867565/150‑1 (MQ=255)
                                                                                                                   ataaACTCTTTGACTTTGTAATTAACGGCCCCGCAAATACCCCCTCAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc                                   <  1:6767672/150‑1 (MQ=255)
                                                                                                                   ataaACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc                                   >  2:7564013/1‑150 (MQ=255)
                                                                                                                       aCTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCtct                               >  2:1338681/1‑148 (MQ=255)
                                                                                                                              tactgtgtAATTAACGGCCCCGCAATTAACCCCTCCACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgt                        <  2:2651795/146‑1 (MQ=255)
                                                                                                                              gACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgt                        <  1:779335/150‑1 (MQ=255)
                                                                                                                               aCAGTGTAATTAACGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgtg                       <  1:11983/150‑1 (MQ=255)
                                                                                                                                  gTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCa                    >  2:3365926/1‑150 (MQ=255)
                                                                                                                                  gTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCa                    >  1:2937053/1‑150 (MQ=255)
                                                                                                                                   tGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCaa                   >  2:1115864/1‑150 (MQ=255)
                                                                                                                                   tGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCaa                   <  1:5025297/150‑1 (MQ=255)
                                                                                                                                      aaTTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCt                >  2:9850638/1‑150 (MQ=255)
                                                                                                                                       aTTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCtt               <  2:8761251/150‑2 (MQ=255)
                                                                                                                                        ttAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGc              >  2:5224489/1‑150 (MQ=255)
                                                                                                                                        ttAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGc              <  1:9886219/150‑1 (MQ=255)
                                                                                                                                          aaCGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCa            <  2:5444308/150‑1 (MQ=255)
                                                                                                                                          aaCGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCa            >  1:1603647/1‑150 (MQ=255)
                                                                                                                                          aaCGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCa            <  2:2066220/150‑1 (MQ=255)
                                                                                                                                              gCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTaa        <  2:3229686/150‑1 (MQ=255)
                                                                                                                                               ccccGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAAc       <  2:8216422/150‑1 (MQ=255)
                                                                                                                                                cccGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACa      >  2:4492175/1‑150 (MQ=255)
                                                                                                                                                cccGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACa      <  1:9618069/150‑1 (MQ=255)
                                                                                                                                                 ccGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAg     <  1:3472506/150‑1 (MQ=255)
                                                                                                                                                 ccGCCATTAACCCCTCAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAg     <  2:8889075/150‑1 (MQ=255)
                                                                                                                                                  cGCCATTAACCCCTGTACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGTAGACAGCGAAAAAAAAGTGACTGAAAGTAAATATAATCTTAATTATCATATTCTTTGTGACAACCTGCCAGTAACAgg    >  1:2088622/1‑150 (MQ=255)
                                                                                                                                                  cGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAgg    >  1:9202789/1‑150 (MQ=255)
                                                                                                                                                  cGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAgg    >  1:2747183/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGA  >  NC_000913/2481671‑2481968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: