Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,850 T→A 9.0% intergenic (‑104/+219) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,8500TA 9.0% 1719.7 / 41.3 566intergenic (‑104/+219)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (192/304);  new base A (61/4);  total (258/308)
Fisher's exact test for biased strand distribution p-value = 1.28e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 575 total aligned reads displayed.

ACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGT  >  NC_000913/2725702‑2725999
                                                                                                                                                     |                                                                                                                                                     
actactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGGTTTTGTCGGTCGGTTTTGTTTTTTTTTTTTTTTTTGGTAATATTTTTGATTTGTTGGATATGATTTTGCAACCtt                                                                                                                                                      >  2:3289615/1‑150 (MQ=255)
actactTGTCAGTTTGCTGTCTGCCGTTACAGTACCTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCtt                                                                                                                                                      >  2:8391346/1‑150 (MQ=255)
  tactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTaa                                                                                                                                                    <  1:6061605/150‑1 (MQ=255)
   actTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAc                                                                                                                                                   <  1:9845367/150‑1 (MQ=255)
   actTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATGATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAc                                                                                                                                                   <  2:4790382/150‑1 (MQ=255)
    ctTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAcc                                                                                                                                                  <  1:7411045/150‑1 (MQ=255)
     ttGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTTTAAAGGAAATAATATACATTTTTTTCATATAATTATTCAAACATAACCa                                                                                                                                                 >  2:96288/1‑150 (MQ=255)
      tGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCAt                                                                                                                                                <  1:3374761/150‑1 (MQ=255)
       gTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCAGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATAATTATTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATg                                                                                                                                               <  2:3501637/150‑1 (MQ=255)
        tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAAAATAATAAATTTTTTTCATAACATTATTAAAACATAACCaaaa                                                                                                                                              >  2:9255066/1‑147 (MQ=255)
        tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGa                                                                                                                                              <  1:1866410/150‑1 (MQ=255)
        tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGa                                                                                                                                              <  1:1321231/150‑1 (MQ=255)
         cAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGaa                                                                                                                                             <  1:770898/150‑1 (MQ=255)
            tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATAATTATGCAACCATAAACAAGAAttt                                                                                                                                          >  1:645698/1‑150 (MQ=255)
             ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                                                         <  2:7862372/150‑1 (MQ=255)
             ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGGTTGTTTATTTTTTTTGTTAAGGGAAAATTATAAAATTTTTTCATATAAATATTGAAACATAAACCAGAATtta                                                                                                                                         >  1:4596826/1‑150 (MQ=255)
             ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCCTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                                                         <  1:9040281/150‑1 (MQ=255)
              tGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTTAAGGAAATATTATAAAATTTTTTCATATAATTATTCAACCATAAACAATAATttat                                                                                                                                        >  1:975206/1‑149 (MQ=255)
              tGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttag                                                                                                                                        <  2:1711947/150‑1 (MQ=255)
              tGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttag                                                                                                                                        >  2:1009954/1‑150 (MQ=255)
               gCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagt                                                                                                                                       <  2:6815891/150‑1 (MQ=255)
                cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATAATATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagtt                                                                                                                                      >  2:8115148/1‑150 (MQ=255)
                    cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCTGTCGCTATTGTTTATATTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                                                                                                                                  <  2:5851927/150‑1 (MQ=255)
                    cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                                                                                                                                  <  1:88423/150‑1 (MQ=255)
                      gCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTTTTTATTTTTTTTGTAAAGGAAATATTAAAAATTTGTTGCATATAATTATACAAAAATAAACAAGAATTTAGTTATCAgg                                                                                                                                >  1:8145669/1‑150 (MQ=255)
                          ttACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTTTTTAATGAAAAATTATAAATTTGTTTCATATAAATATTCAAACATAAACATGAATTTAGTTAGCAGGaaaa                                                                                                                            >  1:2275865/1‑150 (MQ=255)
                           tccAGTACTTTCTGCCATGCCATTATGTGTCCTGCAGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTTTTTTTTTTGTTAAGGAAATATTATACATTTGTTGGATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAt                                                                                                                           <  2:5943850/148‑1 (MQ=255)
                           tACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAt                                                                                                                           <  2:7175570/150‑1 (MQ=255)
                             cAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGTAACCTTAACCATGAATTTAGTTAGCAGGAAAATgg                                                                                                                         <  1:1461572/150‑1 (MQ=255)
                             cAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGTAACCTTAACCATGAATTTAGTTAGCAGGAAAATgg                                                                                                                         <  1:3499726/150‑1 (MQ=255)
                               gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGtt                                                                                                                       <  2:7957200/150‑1 (MQ=255)
                                tACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACAATGAATTTAGTTAGCAGGAAAATTGTTa                                                                                                                      >  2:9132920/1‑150 (MQ=255)
                                 aCTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTGTAAAGGAAATATTACACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAtt                                                                                                                    <  2:4196978/150‑1 (MQ=255)
                                   tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                                                   <  2:936806/150‑1 (MQ=255)
                                   tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTTGTTAAGGAAATATTATACAATTTTTTCATATCATTATGCAACCATAAACAAGAATTTATTTATGATGAAAAAGGTTAttt                                                                                                                   >  1:9155393/1‑149 (MQ=255)
                                    ttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTga                                                                                                                  <  1:8663697/150‑1 (MQ=255)
                                    ttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTGTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTga                                                                                                                  <  2:7277651/150‑1 (MQ=255)
                                        gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgaggag                                                                                                              <  2:7676419/150‑1 (MQ=255)
                                        gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACAATTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTGTTgaggag                                                                                                              >  2:3735049/1‑150 (MQ=255)
                                         ccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTTCATATCATTATGCAAACTTAACCATGACTTTAGTTAGCAGGAAAATGGTGATTGAGgcgc                                                                                                             >  2:2924660/1‑150 (MQ=255)
                                           atgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCtt                                                                                                           >  2:7402935/1‑150 (MQ=255)
                                            tgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTa                                                                                                          <  2:7028426/150‑1 (MQ=255)
                                             gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                         <  1:4390899/150‑1 (MQ=255)
                                             gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                         <  1:2593869/150‑1 (MQ=255)
                                             gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                         <  1:5070010/150‑1 (MQ=255)
                                              ccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAg                                                                                                        <  1:7904213/150‑1 (MQ=255)
                                               catTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAATGGAATATTATACATTTGTTTCAAATCATTATGCAACCATAACCAAGAATTTATTTAGCAGGAAAATGGTTATTTTGGGGCTTaatg                                                                                                       >  2:2760159/1‑148 (MQ=255)
                                                atTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAATGAAAAATTAAAAAATTGTTTCAAAAAAATATTCAAACATAAACATGAATGTAGTTAGCAGGAAAATTGTTATTGATGGGGTTAAGGa                                                                                                      >  2:5181055/1‑150 (MQ=255)
                                                  tATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTGTTTTTTATTTTTTTTTTTAATGGAAAATTATAAAATTTTTTGAAATAAATATTGAAACAAAAACAATAAATTAGTTTTAAGGAAAAATGTTATTGTTGAGCATAAggtaa                                                                                                    >  1:7330851/1‑147 (MQ=255)
                                                   aTGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATaa                                                                                                   <  1:6789480/150‑1 (MQ=255)
                                                     gTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAAt                                                                                                 <  2:8215375/150‑1 (MQ=255)
                                                      tctcCTGCCGTAATCCGATGCTTGTGTCGGTCGCTTTTGGTTATTTTGTTTGTAAAGGCACTATTATACATTTGTTGCATAGTATTATGCAACCATCACCATGAATTTAGTTAGCAGGAAAATGGGTATGGAGGAGCTTAAGGATaaagt                                                                                                >  2:8922925/1‑148 (MQ=255)
                                                         ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                             <  1:9458147/150‑1 (MQ=255)
                                                         ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                             <  1:3386737/150‑1 (MQ=255)
                                                         ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                             <  1:5352491/150‑1 (MQ=255)
                                                          cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                                            <  1:5586088/150‑1 (MQ=255)
                                                          cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                                            <  2:2132081/150‑1 (MQ=255)
                                                           tGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTgg                                                                                           <  2:6543633/150‑1 (MQ=255)
                                                             cagTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAATTTTTTTGTAAAGGAAATATTATACATTTGGTGCAAAACATTATTAAACCATAACCATTAAAATAGTTAGCAGGAAAAAGGTTATTGAGGATCTTAAGGATAAATTTCTGTTa                                                                                         >  2:3874236/3‑150 (MQ=255)
                                                                  aTCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAaggag                                                                                    <  1:5676466/150‑1 (MQ=255)
                                                                    ccGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAggagga                                                                                  >  2:1581171/1‑150 (MQ=255)
                                                                         gCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGt                                                                             <  2:2275865/150‑1 (MQ=255)
                                                                                tcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTTCATATCATTATTCAAACATAAACATTAATTTAGTTAGCAGGAAAATGGTTATTTAGGAGCTTAAGGAAAAATTTCTGGTAAGGAGGACACCTATGGAAg                                                                      >  2:9813876/1‑150 (MQ=255)
                                                                                   gtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTgg                                                                   <  2:6910773/150‑1 (MQ=255)
                                                                                     cgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGGAGTGGGc                                                                 <  2:6081617/150‑1 (MQ=255)
                                                                                        ttttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGACGTGGGCAAg                                                              >  2:3587024/1‑150 (MQ=255)
                                                                                        ttttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCAAGAATTTATTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAg                                                              >  1:9300516/1‑150 (MQ=255)
                                                                                          ttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGtt                                                            >  2:3408833/1‑150 (MQ=255)
                                                                                             tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                                         >  1:4930961/1‑150 (MQ=255)
                                                                                             tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                                         <  1:7119342/150‑1 (MQ=255)
                                                                                                 ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAg                                                     >  1:2794140/1‑150 (MQ=255)
                                                                                                   ttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTgggggaagc                                                   <  2:2527069/150‑6 (MQ=255)
                                                                                                   ttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGcc                                                   >  1:4823510/1‑150 (MQ=255)
                                                                                                   ttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTAAAGGATAAATAGCGGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGcc                                                   >  2:539517/1‑150 (MQ=255)
                                                                                                       ttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTAt                                               <  1:1090694/150‑1 (MQ=255)
                                                                                                        tGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATc                                              <  2:4994826/150‑1 (MQ=255)
                                                                                                          tAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACTTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCg                                            >  2:3185283/1‑150 (MQ=255)
                                                                                                          tAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCg                                            >  1:5818340/1‑150 (MQ=255)
                                                                                                            aaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAATTTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGtt                                          >  1:8313041/1‑150 (MQ=255)
                                                                                                             aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTg                                         <  2:4104015/150‑1 (MQ=255)
                                                                                                             aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTg                                         <  2:1301995/150‑1 (MQ=255)
                                                                                                              ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                                        >  2:6322604/1‑150 (MQ=255)
                                                                                                              ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                                        >  2:3992462/1‑150 (MQ=255)
                                                                                                                  atatTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGaa                                    >  1:7563486/1‑150 (MQ=255)
                                                                                                                       ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg                               <  1:4096222/150‑1 (MQ=255)
                                                                                                                       ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg                               <  1:2915701/150‑1 (MQ=255)
                                                                                                                       ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg                               <  1:7459360/150‑1 (MQ=255)
                                                                                                                        taCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGc                              >  2:1858123/1‑150 (MQ=255)
                                                                                                                                ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGTCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                      <  1:3083801/150‑1 (MQ=255)
                                                                                                                                ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                      <  1:2100043/150‑1 (MQ=255)
                                                                                                                                ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                      <  2:3064316/150‑1 (MQ=255)
                                                                                                                                ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                      <  1:4269706/150‑1 (MQ=255)
                                                                                                                                ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                      <  2:4272460/150‑1 (MQ=255)
                                                                                                                                ttgCATAACATTATGCAACCATAACCATGAATATAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                      <  2:8602913/150‑1 (MQ=255)
                                                                                                                                      atCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtat                <  1:8560788/150‑1 (MQ=255)
                                                                                                                                         aTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAg             >  1:10016806/1‑150 (MQ=255)
                                                                                                                                          ttATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTGATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGa            >  2:4520568/1‑150 (MQ=255)
                                                                                                                                                cAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACgcgc       <  1:4519831/150‑1 (MQ=255)
                                                                                                                                                 aaCCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCa      >  1:3814802/1‑150 (MQ=255)
                                                                                                                                                   ccTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGc    >  1:627541/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGT  >  NC_000913/2725702‑2725999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: