Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,004,963 T→A 6.9% E275V (GAG→GTG)  ygeV ← putative sigma‑54‑interacting transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,004,9630TA 6.9% 1672.8 / 6.9 562E275V (GAG→GTG) ygeVputative sigma‑54‑interacting transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (187/303);  new base A (59/2);  total (257/305)
Fisher's exact test for biased strand distribution p-value = 1.99e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 571 total aligned reads displayed.

TAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATGAA  >  NC_000913/3004814‑3005112
                                                                                                                                                     |                                                                                                                                                     
  aaGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCg                                                                                                                                                     >  1:9905554/1‑150 (MQ=255)
  aaGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCg                                                                                                                                                     <  2:8317464/150‑1 (MQ=255)
  aaGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCg                                                                                                                                                     >  1:5656861/1‑150 (MQ=255)
    gggTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGcc                                                                                                                                                   <  2:8557767/150‑1 (MQ=255)
     ggTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCAAAACAAGGCACACGcca                                                                                                                                                  >  2:974620/1‑150 (MQ=255)
     ggTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCCCTCGccc                                                                                                                                                  >  1:3195985/1‑149 (MQ=255)
     ggTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGcca                                                                                                                                                  >  1:380718/1‑150 (MQ=255)
       tttATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGccaac                                                                                                                                                >  1:1348138/1‑150 (MQ=255)
        ttATTCCTTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCATTACCCGTGCCGCTTTCACCAACCACCATAACCCTGGATGGCCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTCCCGCATAACACGCCACTCGccaacc                                                                                                                                               <  2:7315368/150‑1 (MQ=255)
          aTTCCGTCCTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCATTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACACGccaaccaa                                                                                                                                             <  2:637083/150‑1 (MQ=255)
            tCCGTCTTCCGCTCAACTTATGGAGGGCACGGGCGACGACTTCTTGACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAAtt                                                                                                                                           <  2:2263024/150‑1 (MQ=255)
             ccGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCCCATAACACCGCCCTCGCCAACCAAATg                                                                                                                                          >  1:8688121/1‑150 (MQ=255)
               gTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGtt                                                                                                                                        <  2:6508851/150‑1 (MQ=255)
                  ttCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCaa                                                                                                                                     >  1:781400/1‑150 (MQ=255)
                  ttCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAAAACGGCACTCGCCAACCAAATGTTCaa                                                                                                                                     >  1:4935500/1‑150 (MQ=255)
                   tCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAat                                                                                                                                    <  1:8492563/150‑1 (MQ=255)
                       cTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTTTTCAataatc                                                                                                                                >  2:7142559/1‑147 (MQ=255)
                       cTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTAGCCAAACAAATTTTAAatattc                                                                                                                                >  2:8620738/1‑148 (MQ=255)
                        tCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCg                                                                                                                               <  2:580792/150‑1 (MQ=255)
                            cTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTtc                                                                                                                           <  1:8115753/150‑1 (MQ=255)
                               aTGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTtcatc                                                                                                                        <  1:3452385/150‑1 (MQ=255)
                               aTGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAAAACGGCACTCGCCAACCAAATGTTCAATATGCGGTtcatc                                                                                                                        >  1:6129560/1‑150 (MQ=255)
                                tGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTtcatca                                                                                                                       <  1:9572141/150‑1 (MQ=255)
                                     tGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGc                                                                                                                  <  1:7131205/150‑1 (MQ=255)
                                       cACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCAt                                                                                                                <  1:5319575/150‑1 (MQ=255)
                                           ggCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGc                                                                                                            <  2:7681206/150‑1 (MQ=255)
                                           ggCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGc                                                                                                            <  2:6908782/150‑1 (MQ=255)
                                            gCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCt                                                                                                           <  2:7374472/150‑1 (MQ=255)
                                            gCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCt                                                                                                           <  2:9197030/150‑1 (MQ=255)
                                            gCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAAAACGGCACTCGCCAACCAATTGTTCAATATGCGGTTGATCACGTGCATTTGCt                                                                                                           >  1:9808514/1‑150 (MQ=255)
                                             cgacgaCTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTa                                                                                                          <  1:3656588/150‑1 (MQ=255)
                                               acgacTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACa                                                                                                        <  1:6574207/150‑1 (MQ=255)
                                                  acTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGaa                                                                                                     <  2:9017871/150‑1 (MQ=255)
                                                  acTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGaa                                                                                                     <  2:2176481/150‑1 (MQ=255)
                                                    ttcttTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGaaaa                                                                                                   <  1:9272303/150‑1 (MQ=255)
                                                       tttACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACt                                                                                                <  2:2843422/150‑1 (MQ=255)
                                                       tttACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACt                                                                                                <  2:8017151/150‑1 (MQ=255)
                                                        ttACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTg                                                                                               <  1:8602683/150‑1 (MQ=255)
                                                        ttACCCGCGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACCGCACTCGCCAACCAAATGTTCAATAATCGGTTCATCAGGTGCCTTTGCTACAGaaaaaaag                                                                                               >  2:9326364/1‑147 (MQ=255)
                                                         tACCCGTGCCGCTTTCACCAACCACCCTAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAAGTGCCGCAAAACACCGCCCCCGCCCACCCAAAGTTCAAAAAGCGGGTCCACACGTGCAGTTGCGACAGAAAAACAgg                                                                                              >  1:6421112/1‑150 (MQ=255)
                                                         tACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCCCAAAACACGGCCCACGCCCCCCAAAATTTCAAAATGCGGGTCATCAGGTGCAATTGATACAGaaaaaaagg                                                                                              >  1:2419591/1‑146 (MQ=255)
                                                         tACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTgg                                                                                              <  2:6225599/150‑1 (MQ=255)
                                                             cGTGCCGCTTTCACCTACCACCATAACGCTGGATGGGCTGGTTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAATACTGGTATg                                                                                          <  2:7561513/150‑1 (MQ=255)
                                                             cGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATg                                                                                          <  1:6664472/150‑1 (MQ=255)
                                                                 ccGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACCGCCCACCCCAACCCAATGTTCAAAATGCGGGTCATCCGGTGCAGTTGCGACAGAAAAACAGGGATGCGag                                                                                      >  1:6071014/1‑149 (MQ=255)
                                                                   gCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCCTAACACGGCACACGCCAACCAAATGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACAGGTATGCGATTg                                                                                    >  1:8962312/1‑150 (MQ=255)
                                                                   gCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTg                                                                                    <  1:5084157/150‑1 (MQ=255)
                                                                    cTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTTATTGCCGCATAACACCGCCCTCGCCCACCAAATTTTCAATAAGCGGTTCATAAGGTGCATTTTCGACAGAAAAACAGGTATTCGCTTgg                                                                                   >  1:2838524/1‑150 (MQ=255)
                                                                          ccaaccaCCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCAAAAAACGGCCCTCCCCAACCAATTGTTAAATATGCGGTTCATAAGGTGCCTGTGCTACAGAAAAACGGGGATTCGATTGGTGAAAc                                                                             >  2:1299515/1‑150 (MQ=255)
                                                                          ccaaccaCCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAAc                                                                             <  1:484730/150‑1 (MQ=255)
                                                                          ccaaccaCCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAAc                                                                             <  1:8353797/150‑1 (MQ=255)
                                                                           caaccaCCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTTATTTCCGCAAAAAAACGCACACGCCAACCAATTGTTAAATATTCGGGTCAACAGGTGCAATTTCTACAGAAAAACAGGTTTGCGGTTGGTGTAACg                                                                            >  1:1215262/1‑150 (MQ=255)
                                                                           caaccaCCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAAATGTTCAATATTCGGTTCATCAGGTGCATTTGCTACAGAAAAAATGGTATGCGATTGGTGAAACg                                                                            >  2:7809551/1‑150 (MQ=255)
                                                                           caaccaCCAAAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTATTAATTGCCGCCAAAAACGGCACAACCCAACCAAAAGTTCAAAATTCCGTTCATCAGGTGACATAGCAACAGAAAAAAAGGTAAGCCAATGGTGaaaag                                                                            >  2:5100885/1‑148 (MQ=255)
                                                                                   aTAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTaaa                                                                    <  1:8282833/150‑1 (MQ=255)
                                                                                    tAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTTCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTaaaa                                                                   >  2:4041384/1‑150 (MQ=255)
                                                                                     aaCGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTaaaaa                                                                  <  1:4089873/150‑1 (MQ=255)
                                                                                     aaCGCTGGACGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTaaaaa                                                                  <  2:3248901/150‑1 (MQ=255)
                                                                                      aCGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAaat                                                                 <  2:2599062/150‑1 (MQ=255)
                                                                                       cGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAaata                                                                <  1:1036757/150‑1 (MQ=255)
                                                                                       cGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAaata                                                                <  2:620416/150‑1 (MQ=255)
                                                                                        gCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAaataa                                                               <  2:8155378/150‑1 (MQ=255)
                                                                                        gCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAaataa                                                               <  1:6738475/150‑1 (MQ=255)
                                                                                              tGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCg                                                         <  2:6613430/150‑1 (MQ=255)
                                                                                              tGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCg                                                         <  2:1943079/150‑1 (MQ=255)
                                                                                               gggCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCATAACACGGCACGCGCCAACCAATTGTTCAAAATGCGGTTCATCAGGTGCAATTTCTACATAAAAACAGGTATGCGATTGGTGAAACCCCATTAAAAATAAATTTCgg                                                        >  2:5386072/1‑150 (MQ=255)
                                                                                               gggCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCgg                                                        <  2:7014699/150‑1 (MQ=255)
                                                                                                ggCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGc                                                       >  2:5308987/1‑150 (MQ=255)
                                                                                                  cTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCATAACACGGCACTCGCCAAACAAATGTTCAATATGCGGTTCATAAGGTGCATTTGCTAAAGAAAAACTGGGATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGccc                                                     >  2:3863545/1‑150 (MQ=255)
                                                                                                   tGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAAAACGGCACTCGCCAACCAAATGTTCAATAATCGGTTCATCAGGTTCAATTGCTAAAGAAAAACAGGTATGCGATTGGTGAAAACCCATTAAAAATAAATGTCGGccca                                                    >  2:1708995/1‑149 (MQ=255)
                                                                                                      gTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGaa                                                 <  1:7508052/150‑1 (MQ=255)
                                                                                                      gTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGaa                                                 <  1:1984422/150‑1 (MQ=255)
                                                                                                          aaTACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTTCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAAAAATTGTCGGCCCAGAAtttt                                             >  2:2456741/1‑150 (MQ=255)
                                                                                                          aaTACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAAATGTTCAATATGCGGTTCATCAGGTGCATTTGCGACAGAAAAACAGGTATGCGATTGGTGAAACGCCAATAAAAATAATTGTCGGCCCGGAATGtt                                             >  2:8866950/1‑150 (MQ=255)
                                                                                                           aTACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTa                                            <  2:5516926/150‑1 (MQ=255)
                                                                                                            tACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTAt                                           <  1:5717813/150‑1 (MQ=255)
                                                                                                            tACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTAt                                           <  2:9423107/150‑1 (MQ=255)
                                                                                                            tACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTAt                                           <  2:4876870/150‑1 (MQ=255)
                                                                                                             aCGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATg                                          <  1:1677497/150‑1 (MQ=255)
                                                                                                             aCGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATg                                          <  1:8856743/150‑1 (MQ=255)
                                                                                                                     tGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGAc                                  <  2:611806/150‑1 (MQ=255)
                                                                                                                       aGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCa                                <  1:6305135/150‑1 (MQ=255)
                                                                                                                           gTTTTAATTGCCGCATAACACGGCACTCGCCAACAAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACATAAAAAATGGTATTCGATTGGTGAAACGCCATTAAAAATAATTGTCGGACATGAATGTTATGCAATTGAACAATGa                            >  2:6847549/1‑150 (MQ=255)
                                                                                                                            ttttAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGAt                           >  1:2211846/1‑150 (MQ=255)
                                                                                                                            ttttAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGAt                           <  2:8238160/150‑1 (MQ=255)
                                                                                                                                 aTTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAAtt                      <  2:467334/150‑1 (MQ=255)
                                                                                                                                  ttGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTc                     >  2:9687679/1‑150 (MQ=255)
                                                                                                                                   tGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCa                    <  2:5039017/150‑1 (MQ=255)
                                                                                                                                     ccGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACt                  >  1:4212095/1‑150 (MQ=255)
                                                                                                                                         aTAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTa              <  2:368671/150‑1 (MQ=255)
                                                                                                                                          tAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTAt             >  1:2890528/1‑150 (MQ=255)
                                                                                                                                          tAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTAt             >  2:335307/1‑150 (MQ=255)
                                                                                                                                              acGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTc         >  2:1019863/1‑150 (MQ=255)
                                                                                                                                              acGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTc         >  1:1507647/1‑150 (MQ=255)
                                                                                                                                               cGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTcc        <  2:3003811/150‑1 (MQ=255)
                                                                                                                                               cGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTcc        >  2:1473411/1‑150 (MQ=255)
                                                                                                                                                ggCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTccc       <  2:5656861/150‑1 (MQ=255)
                                                                                                                                                 gCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCa      >  2:4261602/1‑150 (MQ=255)
                                                                                                                                                   aCTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATg    >  1:5919472/1‑150 (MQ=255)
                                                                                                                                                     tCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATGaa  >  2:4587154/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATGAA  >  NC_000913/3004814‑3005112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: