Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,611 C→A 5.6% intergenic (+66/‑333) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6110CA 5.6% 1548.5 / 51.0 460intergenic (+66/‑333)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base C (268/159);  new base A (11/16);  total (279/181)
Fisher's exact test for biased strand distribution p-value = 2.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 464 total aligned reads displayed.

GCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTT  >  NC_000913/3239463‑3239760
                                                                                                                                                      |                                                                                                                                                     
gCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCa                                                                                                                                                      <  1:1673269/150‑1 (MQ=255)
gCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCa                                                                                                                                                      <  1:6217486/150‑1 (MQ=255)
gCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCa                                                                                                                                                      <  2:8284764/150‑1 (MQ=255)
 cGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAg                                                                                                                                                     <  2:1797091/150‑1 (MQ=255)
   tggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAggg                                                                                                                                                   <  2:6631948/150‑1 (MQ=255)
    ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                                  <  2:9700476/150‑1 (MQ=255)
    ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                                  <  1:2279062/150‑1 (MQ=255)
    ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                                  <  2:5790211/150‑1 (MQ=255)
      tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                                >  1:124689/1‑150 (MQ=255)
      tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                                <  2:3314316/150‑1 (MQ=255)
        tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAgg                                                                                                                                              <  1:4209043/150‑1 (MQ=255)
        tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCACCCTCTCCCTGGAAGGGAGAgg                                                                                                                                              <  1:9971012/150‑1 (MQ=255)
        tttGGCATGCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAgg                                                                                                                                              <  2:7815489/150‑1 (MQ=255)
          tGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAggga                                                                                                                                            <  1:3751967/150‑2 (MQ=255)
             cATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                         >  1:1403486/1‑150 (MQ=255)
                  tGGTGATGACGTTTATTATCAACGCGTGGGTGAATTATCGGCATGATAAGCAGGGGGGTGGATAATTTTTAATCTGCCTACGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                    <  1:5877643/150‑1 (MQ=255)
                  tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                    <  2:390525/150‑1 (MQ=255)
                  tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                    <  2:73998/150‑1 (MQ=255)
                  tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                    >  1:6959842/1‑150 (MQ=255)
                     tgatgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAggg                                                                                                                                 >  1:964427/1‑150 (MQ=255)
                     tgatgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCATCCAGGGAGAGGGTCGGGGTGAggg                                                                                                                                 >  1:4966729/1‑150 (MQ=255)
                       atgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTa                                                                                                                               >  1:3952522/1‑150 (MQ=255)
                        tgaCGTTTATTATCACCGCTGGGTTTATTTTTGGGATTTATAAGCTGGGGGGTGGATAATTTTTAATCTGCCTACGCGGTGTACCCTTACTTTAACTTGAGCACCTTTTTCTCCCTCTCCCTTCCTGGGTGTGGGTCGGGGTGTGGGTaa                                                                                                                              <  1:8224673/150‑1 (MQ=255)
                          aCGTTTATGATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtt                                                                                                                            <  2:6470118/150‑1 (MQ=255)
                            gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                          >  1:7880355/1‑150 (MQ=255)
                                  tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                                                                                    >  2:4494294/1‑150 (MQ=255)
                                          ccTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTg                                                                                                            >  2:2629770/1‑150 (MQ=255)
                                             gggTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGcc                                                                                                         >  1:3017868/1‑150 (MQ=255)
                                                tGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGt                                                                                                      >  1:2670286/1‑150 (MQ=255)
                                                tGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGt                                                                                                      >  1:8495670/1‑150 (MQ=255)
                                                  aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                    >  2:4343312/1‑150 (MQ=255)
                                                    ttATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTccc                                                                                                  >  2:4692736/1‑150 (MQ=255)
                                                    ttATCGGATTGATAAGCCGGGGGGTGTAAAATTTTTAATCTGCATCCGCCGTTTACCCTTACATTAACTTGAGCCCCTTTTTCTCCCTCTCCCTCCCTGGGAGAGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTccc                                                                                                  <  2:5362734/150‑1 (MQ=255)
                                                     tATCGGCATGATAAGCTGCGGGGTGTATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGTTGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                                 <  2:1103662/150‑1 (MQ=255)
                                                          gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGTTGAGGGTAATTATTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                            >  2:8939212/1‑150 (MQ=255)
                                                          gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                            >  2:6756800/1‑150 (MQ=255)
                                                                     ctggggggTGTATATTTTTTAATGTGCCTCCGCCGTGTACCCTGACATTAACATGCGCACCGTTTTCTCCCTCGCCCTTCCCGGGTGTGGGTGGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct                                                                                  <  2:8756544/146‑1 (MQ=25)
                                                                      tggggggTGTATAATTTTTATTCTCCCAATCCCGTGTACCGTGTATTTAACTTGAGCCCGGTTTTCTCCCTCCCCCTTCCCGGTTGTGGGTGGGGGTGCGGGTAAATTTTCGCACCGATGCTGGCCTGGTCCCCTCACCCTAACCCtctc                                                                                 <  1:3165780/147‑1 (MQ=16)
                                                                        cGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTccc                                                                               >  2:418350/1‑150 (MQ=255)
                                                                               tatatTTTTTATTGTCCCTACGCCTTGTACCCTTTATTTAAATTGCGCCCGTTTTCCCCACCCTCCTTGCCGGGTTTTGGGTGGGGTTGGGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                        <  2:3611384/149‑1 (MQ=12)
                                                                               gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCa                                                                                                                                                      <  1:2363731/72‑1 (MQ=255)
                                                                               gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCa                                                                                                                                                      >  2:2363731/1‑72 (MQ=255)
                                                                               gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                        >  2:8112326/1‑150 (MQ=255)
                                                                                 tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                      >  2:6221202/1‑150 (MQ=255)
                                                                                  attttttAATCTGCATAAGCCGTGTACCCTGTCTTTAACATGCGCACCTTTTTCTCCCTCGCCCTTCCTGGGTGTGGGTGGGGTTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGc                                                                     <  2:4878080/148‑1 (MQ=25)
                                                                                    tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                   >  1:7058298/1‑150 (MQ=255)
                                                                                    tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                   <  2:8360942/150‑1 (MQ=255)
                                                                                     ttttAATCTTCATACGCGGTTAACCCTTTAATAAACTTGAGCACGGTTTCCCCCATCTCCCTCCCTGGTTGTGGGTGGGGTTGTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                  <  1:5641213/150‑1 (MQ=16)
                                                                                     ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCCAACGGGGCGAg                                                                  >  2:1653679/1‑150 (MQ=255)
                                                                                     ttttAATCTCCCTACGCGGTTTACCCGGTAATTAACATGCGCACCGTTTACGCCCTCTCCCTTCCTGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                  <  1:7391153/150‑1 (MQ=25)
                                                                                      tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                                 >  1:7578744/1‑150 (MQ=255)
                                                                                         aaccTGCTTACGCGGTGTCCCGTTTAATTAACTTGCGCCCGTTTTTCCCCCCCCCCCGTCCGGGGTGTGGGGGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGa                                                              <  1:8879751/147‑1 (MQ=12)
                                                                                          aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                             >  2:9845635/1‑150 (MQ=255)
                                                                                           tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACt                                                            >  2:1960814/1‑150 (MQ=255)
                                                                                             tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGa                                                          >  2:7283647/1‑150 (MQ=255)
                                                                                             tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGa                                                          >  1:8781879/1‑150 (MQ=255)
                                                                                              gCCTACGCCGTGTACCCTGTCATTAACATGACCACCTTTTACCCCCTCTCCCTCCCAGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAc                                                         <  1:7273269/150‑1 (MQ=25)
                                                                                              gCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAc                                                         >  2:6053094/1‑150 (MQ=255)
                                                                                               ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                        >  2:7097058/1‑150 (MQ=255)
                                                                                               ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                        <  2:6571559/150‑1 (MQ=255)
                                                                                                cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                       >  2:9507887/1‑150 (MQ=255)
                                                                                                cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                       >  1:4794109/1‑150 (MQ=255)
                                                                                                 tAAGCGGTGTACCCTGTCATTAACTTGAGCACCTTTTTCTCCCTCTCCCTTCAGGGGAGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                      <  2:1387611/150‑1 (MQ=25)
                                                                                                 tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                      >  2:6302472/1‑150 (MQ=255)
                                                                                                 tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                      >  2:3189187/1‑150 (MQ=255)
                                                                                                     ccGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTcc                                                  >  2:2150780/1‑150 (MQ=255)
                                                                                                       gtgtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtt                                                >  2:7037168/1‑150 (MQ=255)
                                                                                                         gtccccGGTCTTTACCATGCGCCCCGTTGTCGCCCTCGCCCTGCCCGGGAGTGGGTGGGGGTGGGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttt                                              <  2:5639620/147‑1 (MQ=21)
                                                                                                            cccTGTCTTTAACCGGCGCCCCGTTTTCTCCCTCGCCCTGCGTGGTTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTg                                           <  2:7921435/150‑1 (MQ=21)
                                                                                                              cTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                         <  1:9855315/150‑1 (MQ=255)
                                                                                                                 tCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgtt                                      >  1:2736529/1‑150 (MQ=255)
                                                                                                                    ttAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATTTTGtc                                   >  2:7589832/1‑150 (MQ=255)
                                                                                                                    ttAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAAAGGGGCGAGGGGACTGACCGAGTCCTTTTTTTTtgttgta                                   >  1:865427/1‑149 (MQ=255)
                                                                                                                       aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatc                                >  1:1026413/1‑150 (MQ=255)
                                                                                                                       aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatc                                >  1:640576/1‑150 (MQ=255)
                                                                                                                       aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATTTTGtaata                                >  1:3565950/1‑149 (MQ=255)
                                                                                                                         aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                              >  2:1295424/1‑150 (MQ=255)
                                                                                                                         aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTACCCAAACGGGGCGAGGGGACTGACCGAGTCGTTTTTTTTTGTTTtcatcat                              >  1:5404746/1‑149 (MQ=25)
                                                                                                                         aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTAGCACCGATGCTGGCCTGTTACCCTCACCCGAACCCACACCCCAAACCGGGCGAGGGGACTGACCGAGTCCTTTTTTTGTGTTGTCATCAg                              >  1:9359627/1‑150 (MQ=255)
                                                                                                                          tGAGCACGTTTTACTCCCTCCCCCTCCCTGGTTGTGGGGGGGGTTTTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGt                             <  1:5920530/150‑1 (MQ=25)
                                                                                                                           gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                            >  1:2540201/1‑150 (MQ=255)
                                                                                                                                 cGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCACACCCAAACCCACACCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATTTTGTCATCAGTCTGGAAg                      >  1:447468/1‑150 (MQ=255)
                                                                                                                                   ttttCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGcc                    >  2:189729/1‑150 (MQ=255)
                                                                                                                                      tccccctctccctCCCCGGGAGCGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCa                 <  1:3346398/147‑1 (MQ=37)
                                                                                                                                       ctccctctccctTCCAGGGAGTGGGTCGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGATGCCGCAc                <  2:1552341/150‑1 (MQ=255)
                                                                                                                                        tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAATCGGGGCGAGGGGACTGACCGAGACCTTTTTTGAGGTGGTCATCCGGCTGGAAGCCGCACg               >  2:8541414/1‑150 (MQ=25)
                                                                                                                                          cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCCTCAGTCTGGAAGCCGCACGtt             >  1:5133982/1‑150 (MQ=255)
                                                                                                                                          cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCTAACCCTCTCCCCAAAAGGGGCGAGGGGACTGACCGAGTACTTTTTTTATGTTTTGATCAGTCTTGAAGCCGCACGtt             >  1:1982952/1‑150 (MQ=21)
                                                                                                                                            ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACCGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg            >  1:9430081/1‑150 (MQ=255)
                                                                                                                                            ctctaactTCCAGGGAGACGTTCGGGGTTAGGGTAATGCTTCGCACCGAAGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGCGGGGACTCACCCTGTCCTTGTTTGATGTTGTCCTCAGTCTTGCAGCCGCACGTTg            >  2:9281220/7‑150 (MQ=21)
                                                                                                                                             tctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg           >  2:3104853/1‑150 (MQ=255)
                                                                                                                                             actccctTCCCGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg           <  2:9113575/149‑1 (MQ=255)
                                                                                                                                               tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt         >  1:8314248/1‑150 (MQ=255)
                                                                                                                                               tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGGTGGCt         >  2:3675005/1‑150 (MQ=255)
                                                                                                                                               tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTGGTCATCAGTCTGGAAGCCGCACGTTGGCt         >  2:864475/1‑150 (MQ=255)
                                                                                                                                               tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTGTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt         >  2:5416885/1‑150 (MQ=255)
                                                                                                                                                   ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCCCCCAAACCCACACCCCCAACCGGGCGCGGGGCCGGACCGAGGCCTGTTTTGATGTTGTCATCAGTCTGGAAGCCCCCCGGGGGCGTTAt     >  1:4284853/1‑150 (MQ=255)
                                                                                                                                                    tCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGc          <  2:523069/144‑1 (MQ=255)
                                                                                                                                                    tCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAtt    >  1:4744116/1‑150 (MQ=255)
                                                                                                                                                    tCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCCTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAtt    >  1:5969436/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTT  >  NC_000913/3239463‑3239760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: