Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,721 A→C 6.3% intergenic (+72/+30) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,7210AC 6.3% 1708.1 / 21.1 580intergenic (+72/+30)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (358/171);  new base C (4/47);  total (362/218)
Fisher's exact test for biased strand distribution p-value = 1.88e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 623 total aligned reads displayed.

TGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCGGC  >  NC_000913/3608572‑3608870
                                                                                                                                                     |                                                                                                                                                     
tggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaa                                                                                                                                                       <  2:9013517/150‑1 (MQ=255)
 ggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                                                      >  2:8307048/1‑150 (MQ=255)
  gctggcAGTGCTGGCAGCTCCGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGAGTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaaa                                                                                                                                                     <  1:2427883/150‑1 (MQ=255)
  gctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaaa                                                                                                                                                     >  1:1905194/1‑150 (MQ=255)
    tggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                                                   >  1:3782217/1‑150 (MQ=255)
        aGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcggc                                                                                                                                               >  2:1588089/1‑150 (MQ=255)
          tGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCAt                                                                                                                                             >  2:185189/1‑150 (MQ=255)
            cTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCg                                                                                                                                           >  1:8969882/1‑150 (MQ=255)
                         gggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGcc                                                                                                                              >  1:2720614/1‑150 (MQ=255)
                         gggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGcc                                                                                                                              >  1:10011040/1‑150 (MQ=255)
                          ggCGACGGTGCTGGTGACAGCGAATGCGTTAATCTTGTTGCGCTGGAGATACGGCAAACCTTTGGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGTGTGGTTTTGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                                             <  2:8968279/150‑1 (MQ=255)
                          ggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                                             >  1:9762738/1‑150 (MQ=255)
                          ggCGACGGTGCGGGTGACAGCGAATGCGTTAAGTTTGTTGCGCAGGAGATAAGGCAAACCGATCGCAAAATTGAGCGCGATCGGTCCCCACGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                                             <  2:8237473/150‑1 (MQ=39)
                                ggTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCa                                                                                                                       >  2:3051143/1‑150 (MQ=255)
                                  tGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAAc                                                                                                                     >  2:239497/1‑150 (MQ=255)
                                    cTGGTGACAGCGATTGCGTTAAGATTGTTGCGCAGTAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTCGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCg                                                                                                                   <  2:1143963/150‑1 (MQ=39)
                                          aCAGCTAATGCGTTAATATTGTTGCGCAGTATATAAGCCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTTGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                                             <  2:4851694/150‑1 (MQ=38)
                                            aGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACg                                                                                                           >  1:1179854/1‑150 (MQ=255)
                                            aGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACg                                                                                                           >  1:5992134/1‑150 (MQ=255)
                                            aGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACg                                                                                                           >  1:4782659/1‑150 (MQ=255)
                                            aGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACg                                                                                                           >  2:7106456/1‑150 (MQ=255)
                                             gCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGa                                                                                                          >  2:4237974/1‑150 (MQ=255)
                                               gAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAt                                                                                                        >  2:4623864/1‑150 (MQ=255)
                                                 aTGCGTTAATATTTTTGCGCAGGAGATACGGCAAACCGAGCGCAACCTTGAGCGGGATGGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCa                                                                                                      <  2:5749977/150‑1 (MQ=38)
                                                    cGTTATGTTTTTTGCGCTGTTTATAAGGCAAACCGCTCCAAACTTTTCGCGCTATGGTCCCCCGCGCCCCTGTGGGTTGTGGTTTTGGGTGTGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAAt                                                                                                   <  1:5442648/150‑1 (MQ=255)
                                                     gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                                                  >  1:8564620/1‑150 (MQ=255)
                                                      ttAAGATTGTTGCGCTGGATATACGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                                                 <  1:9979228/150‑1 (MQ=39)
                                                        aaGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACg                                                                                               >  2:9251641/1‑150 (MQ=255)
                                                          gATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGAt                                                                                             >  1:1571760/1‑150 (MQ=255)
                                                           aTTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATa                                                                                            >  2:9125925/1‑150 (MQ=255)
                                                            ttgttgCGCAGTAGATATGGCAAACCGATCGAAACTTTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTTGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                                           <  2:3219993/150‑1 (MQ=39)
                                                            tggttgCGCTGGAGATACGCAAAACCGATCGAAACTTTGCGCGCTAGGGGCCCCCTCGCCCCTGTGGGGTGGGGGTTTGGGTGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                                           <  1:8097198/148‑1 (MQ=255)
                                                              gttgCTCTGGATATAAGGCAAACCGATCGAAACTTTGCGCGGGTTGGGCCCCCTCGCCCCTGTGGGGTGTGGGTTGGGGTGTGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAgg                                                                                         <  1:9479451/150‑1 (MQ=255)
                                                                   gcAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTc                                                                                    >  2:6864138/1‑150 (MQ=255)
                                                                    cAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTcc                                                                                   >  2:6604009/1‑150 (MQ=255)
                                                                     aGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCa                                                                                  >  2:8908868/1‑150 (MQ=255)
                                                                            aaGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtct                                                                           >  2:6342490/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                        >  1:4256165/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                        >  1:1484265/1‑150 (MQ=255)
                                                                                cacaCCGCTGGCACCATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATACCGATAAGGCAGTCCATCCGTCTCttt                                                                       <  2:9857046/147‑1 (MQ=38)
                                                                                aaaaCCGATCGAAAAATTGCGCGCGATCGGCCCCCCCGCCCCTGTGGGTAGTGGTTTTGGGTGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCttt                                                                       <  1:2954377/149‑1 (MQ=255)
                                                                                 accccGATCGAAACATTGTGCGCGACCGGTCCCCCCGCCCCTCTGGGGAGTGGGTTCGGGTGCGGGGAACAGGCGGCATCGAAGCCAATCAGCCCCTATCACCCGCCTTTACGCATCAAATACCGATAAGGCAGTCCATCCGTCTCTTTa                                                                      <  2:1223852/147‑1 (MQ=255)
                                                                                 acaCCGATCGCACCTTTGAGCGCGAGGGGCCCCCCCCCCCCTCTGGGTAGTGGTTTTGGGTGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                                                      <  2:3512417/148‑1 (MQ=255)
                                                                                 aaaCCTTTCGCAACATTGTGGGCTATGGGTCCCCGCGCCCGTTTGGGGTGTGGTTTTGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                                                      <  1:6870286/150‑1 (MQ=255)
                                                                                 aaaCCTATCGCAACTTTTCGCGCTATCGGTCCCCCCGCCCCTCTGGGTTGTGGGTTTGGGTGAGGTGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                                                      <  1:8307147/150‑1 (MQ=255)
                                                                                 aaaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                                                      >  2:5554577/1‑150 (MQ=255)
                                                                                     cGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                                                                  >  1:5736124/1‑150 (MQ=255)
                                                                                     cGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTGAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGACTCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                                                                  >  2:342927/1‑150 (MQ=255)
                                                                                      gATCGCAACTTTGCGCGCGTTCGGTCCCCTCGCCCCGCTGGGGAGTGGGTTGGGGTGCGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                                                 <  1:7023684/150‑1 (MQ=255)
                                                                                      gATCGCAACTTTGCGCGCGATCGGTCCCCGCGCCCCTCGGGGGTGTGGGTTCGGGTTCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                                                 <  2:403984/150‑1 (MQ=255)
                                                                                      gATCGCAACTTGGAGCGCGATCGGCCCCCTCGCCCCTCTGGGGAGTGGTTTGGGGTGCGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                                                 <  1:3557233/150‑1 (MQ=255)
                                                                                      gATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                                                 >  2:6311075/1‑150 (MQ=255)
                                                                                         ctcAACTTTGAGCGCGAGCGGTCCCCTCGCCCCTGTGGGGTGTGGTTTCGGGTGAGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAg                                                              <  1:5182249/148‑1 (MQ=255)
                                                                                         ctaaaCTTTGCGCGCGATGGGTCCCCTCGCCCCTGTGGGGTGTGGTTTTGGGTGCGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAg                                                              <  1:2198173/147‑1 (MQ=255)
                                                                                           cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                                            >  2:9730986/1‑150 (MQ=255)
                                                                                           cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                                            >  1:5057816/1‑150 (MQ=255)
                                                                                              aaTTGCGCGCGATCGGTCCCCTCGCCCCTGTGGGGTGTGGGTTTGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgt                                                         <  1:850162/149‑1 (MQ=255)
                                                                                                ttGTGCGCGATGGGCCCCCCCGCCCCTGTGGGGTGTGGTTTTGGGTTAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtgt                                                       <  2:8476005/150‑1 (MQ=255)
                                                                                                ttGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtgt                                                       >  2:1533442/1‑150 (MQ=255)
                                                                                                   aGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTcc                                                    >  2:8725029/1‑150 (MQ=255)
                                                                                                    gcgcGATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCa                                                   <  2:5090841/150‑1 (MQ=255)
                                                                                                        gttCGGTCCCCTCGCCCCTGGGGGGTGTGGGTTTGGGTGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                                               <  1:674086/148‑1 (MQ=255)
                                                                                                        gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                                               >  1:9038140/1‑150 (MQ=255)
                                                                                                        gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                                               >  1:727612/1‑150 (MQ=255)
                                                                                                           cctaacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGt                                            >  2:9770793/6‑150 (MQ=255)
                                                                                                           cGGGCCCCGCGCCCCTCTGGGGTGTGGGTTCGGGTGCGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGt                                            <  2:2874325/150‑1 (MQ=255)
                                                                                                            ggTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTa                                           >  1:6395753/1‑150 (MQ=255)
                                                                                                               ccccGCGCCCCTCTGGGGTTTGGGTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaa                                        <  1:5793880/150‑1 (MQ=255)
                                                                                                                  cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAc                                     >  1:3965820/1‑150 (MQ=255)
                                                                                                                   tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAcc                                    >  2:6100030/1‑150 (MQ=255)
                                                                                                                     gCCCCTCTGGGGTGGGGTTTTGGTTGTGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa                                  <  1:2087126/150‑1 (MQ=255)
                                                                                                                      ccccTGTGGGGTGTGGGTTTGGGTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCACATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAg                                 <  1:8798450/150‑1 (MQ=255)
                                                                                                                       cccagaggggCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAgg                                >  2:647339/7‑150 (MQ=255)
                                                                                                                         ctctGGGGCGTGGGTTCGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTCTCAACCGCCTTTACGAATCAACTAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTGCGTGTTCCATAAAGGTACAAAACCCAGGaa                              <  2:8049491/150‑1 (MQ=255)
                                                                                                                         ctctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGaa                              >  1:7791739/1‑150 (MQ=255)
                                                                                                                         ctctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAACGTACAAAACCCAGGaa                              >  2:7009988/1‑150 (MQ=255)
                                                                                                                                gagaGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGACTATcgcg                       >  1:4118992/1‑150 (MQ=255)
                                                                                                                                 agcgggTTCGGGTGAGGGTACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCgg                      <  1:8672994/147‑1 (MQ=255)
                                                                                                                                 agagGGTTCGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCgg                      <  1:6603023/150‑1 (MQ=255)
                                                                                                                                 agagGGTTAGGGTGAGGGGAAAAGGCGTCATCGAAGCCAATCAGCCCATATCAACCGCCTTTAAGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCAAGGAATATCGAgg                      >  2:4366530/1‑150 (MQ=255)
                                                                                                                                 agagGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCgg                      >  2:7732033/1‑150 (MQ=255)
                                                                                                                                  gtgggTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCggg                     <  2:9422944/148‑1 (MQ=255)
                                                                                                                                    gggTTAGGTTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTa                   <  2:3778308/150‑1 (MQ=255)
                                                                                                                                    gggTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTa                   <  2:7894209/150‑1 (MQ=255)
                                                                                                                                     ggTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATGAAGGTACAAAACCCAGGAATATCGCGGGTAg                  >  2:2907728/1‑150 (MQ=255)
                                                                                                                                       ttcgggTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTg                <  2:2075509/147‑1 (MQ=255)
                                                                                                                                         aGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGc              >  2:764286/1‑150 (MQ=255)
                                                                                                                                           ggTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCGTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCg            <  1:3175585/150‑1 (MQ=255)
                                                                                                                                            gTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCgg           >  2:8653620/1‑150 (MQ=255)
                                                                                                                                            gTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCgg           >  2:1800499/1‑150 (MQ=255)
                                                                                                                                             tGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGa          >  2:2706428/1‑150 (MQ=255)
                                                                                                                                               cgggtAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtc        <  2:8805391/149‑1 (MQ=255)
                                                                                                                                               aGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtc        <  1:3385091/150‑1 (MQ=255)
                                                                                                                                               aGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtc        <  2:5290597/150‑1 (MQ=255)
                                                                                                                                                ggggAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcg       >  2:7546917/1‑150 (MQ=255)
                                                                                                                                                 gggACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgt      <  2:9597958/150‑1 (MQ=255)
                                                                                                                                                 gggAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgt      >  1:9276947/1‑150 (MQ=255)
                                                                                                                                                  ggAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGATCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgtc     >  2:730806/1‑150 (MQ=255)
                                                                                                                                                  gcaaaaGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgtc     <  1:2021121/148‑1 (MQ=255)
                                                                                                                                                    aaaaGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCgg   >  2:2289552/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCGGC  >  NC_000913/3608572‑3608870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: