Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 702,330 Δ1 bp 10.9% coding (422/1149 nt) nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913702,3300C.10.9% 781.9 / 98.5 229coding (422/1149 nt)nagAN‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base C (101/103);  new base . (8/17);  total (109/120)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 246 total aligned reads displayed.

CCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAG  >  NC_000913/702181‑702479
                                                                                                                                                     |                                                                                                                                                     
 cacaATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGcc                                                                                                                                                      >  1:1024860/1‑150 (MQ=255)
    aaTCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGACTTCGACCAGCGCGGCATCAGGCTTACGCACACACTTCGGATGATGGGTGCCttt                                                                                                                                                   >  2:2458338/1‑150 (MQ=255)
      tCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCttttt                                                                                                                                                 <  1:1052423/150‑1 (MQ=255)
      tCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCttttt                                                                                                                                                 <  2:1289152/150‑1 (MQ=255)
         cGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTa                                                                                                                                              >  2:26728/1‑150 (MQ=255)
         cGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTa                                                                                                                                              <  1:3561220/150‑1 (MQ=255)
         cGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTa                                                                                                                                              <  1:2730933/150‑1 (MQ=255)
          ggCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTAc                                                                                                                                             <  1:785270/150‑1 (MQ=255)
                tGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTAGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGAtt                                                                                                                                       >  1:2087661/1‑150 (MQ=255)
                 gCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTc                                                                                                                                      >  1:292226/1‑150 (MQ=255)
                  ccAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCa                                                                                                                                     <  1:3849135/150‑1 (MQ=255)
                  ccAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCa                                                                                                                                     <  1:2274788/150‑1 (MQ=255)
                   cAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCTTTTTTTACCAGATTCAGc                                                                                                                                   <  1:585966/150‑1 (MQ=255)
                     gTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGcc                                                                                                                                  >  1:2190970/1‑150 (MQ=255)
                       ttGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCAc                                                                                                                                >  2:3235201/1‑150 (MQ=255)
                       ttGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCAc                                                                                                                                <  2:25918/150‑1 (MQ=255)
                          cTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGa                                                                                                                             <  2:3319575/150‑1 (MQ=255)
                          cTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGa                                                                                                                             <  2:1809720/150‑1 (MQ=255)
                           tgatgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGAc                                                                                                                            >  2:30632/1‑150 (MQ=255)
                                  ttCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGa                                                                                                                    <  2:887027/150‑1 (MQ=255)
                                  ttCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTAACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAg                                                                                                                     >  1:978766/1‑150 (MQ=255)
                                      gCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGc                                                                                                                 >  1:1800460/1‑150 (MQ=255)
                                      gCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGATTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGc                                                                                                                 <  1:2553208/150‑1 (MQ=255)
                                         ggAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGa                                                                                                              <  1:1480536/150‑1 (MQ=255)
                                         ggAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGa                                                                                                              <  1:2901371/150‑1 (MQ=255)
                                          gAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGAc                                                                                                             >  1:490783/1‑150 (MQ=255)
                                           aaCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGAcc                                                                                                            >  2:1853146/1‑150 (MQ=255)
                                           aaCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGAcc                                                                                                            <  1:3117854/150‑1 (MQ=255)
                                            aCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCt                                                                                                           <  1:1145922/150‑1 (MQ=255)
                                            aCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGAAGACCt                                                                                                           >  1:3828579/1‑150 (MQ=255)
                                              cATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTaa                                                                                                         <  2:3640159/150‑1 (MQ=255)
                                              cATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTaa                                                                                                         <  2:3428516/150‑1 (MQ=255)
                                              cATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTaa                                                                                                         <  2:1544506/150‑1 (MQ=255)
                                               aTTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTAAGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAAc                                                                                                        >  2:1400535/1‑150 (MQ=255)
                                                 ttCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGcc                                                                                                     <  2:2149718/150‑1 (MQ=255)
                                                  tCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGcc                                                                                                     <  1:1492432/150‑1 (MQ=255)
                                                   ccGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCTCGGACCTTCCAGATGCAGACCTAACGCCt                                                                                                    <  2:320316/150‑1 (MQ=255)
                                                        gCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTc                                                                                               <  1:2616455/150‑1 (MQ=255)
                                                        gCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTc                                                                                               <  1:1879797/150‑1 (MQ=255)
                                                               tCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCGTTTTTTTACCAGATTCAGCCTCGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGtt                                                                                        >  2:1591349/1‑150 (MQ=255)
                                                               tCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGtt                                                                                        >  2:1060555/1‑150 (MQ=255)
                                                               tCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGtt                                                                                        >  1:4030783/1‑150 (MQ=255)
                                                               tCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGtt                                                                                        >  2:497709/1‑150 (MQ=255)
                                                               tCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGtt                                                                                        >  2:499420/1‑150 (MQ=255)
                                                                   tttGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGAGGTTTTGc                                                                                    >  2:3619956/1‑150 (MQ=255)
                                                                     tGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCa                                                                                  <  2:2951481/150‑1 (MQ=255)
                                                                       gTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCATCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAgg                                                                                >  1:569939/1‑150 (MQ=255)
                                                                        tAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTTCCAGGt                                                                               >  1:2466727/1‑150 (MQ=255)
                                                                        tAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGt                                                                               >  2:1732359/1‑150 (MQ=255)
                                                                           tGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCGTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACt                                                                            >  2:2298178/1‑150 (MQ=255)
                                                                              cGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTcgc                                                                         <  2:3868967/150‑1 (MQ=255)
                                                                              cGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTcgc                                                                         <  1:2047637/150‑1 (MQ=255)
                                                                               gTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTcgcg                                                                        >  2:1178040/1‑150 (MQ=255)
                                                                                 cGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCa                                                                      >  2:2263923/1‑150 (MQ=255)
                                                                                 cGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCa                                                                      <  1:1464859/150‑1 (MQ=255)
                                                                                     gTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAAc                                                                  <  1:26728/150‑1 (MQ=255)
                                                                                       tttCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAAcgc                                                                >  2:3145165/1‑150 (MQ=255)
                                                                                           acacaGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCAc                                                            <  2:1784553/150‑1 (MQ=255)
                                                                                            cacaGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGc                                                          >  1:1395030/1‑150 (MQ=255)
                                                                                              caGTAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGTATTTTGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGcc                                                         <  1:2850185/150‑1 (MQ=255)
                                                                                              caGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTTTGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGcc                                                         <  1:3571653/150‑1 (MQ=255)
                                                                                               aGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGccc                                                        >  1:752514/1‑150 (MQ=255)
                                                                                                 gAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTg                                                      >  1:892497/1‑150 (MQ=255)
                                                                                                 gAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTg                                                      >  1:2766057/1‑150 (MQ=255)
                                                                                                 gAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTg                                                      >  1:3022341/1‑150 (MQ=255)
                                                                                                  aaaTCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGtt                                                    <  2:1172609/150‑1 (MQ=255)
                                                                                                   aaTCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGtt                                                    <  1:3144146/150‑1 (MQ=255)
                                                                                                       gACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcat                                                <  1:1569602/150‑1 (MQ=255)
                                                                                                       gACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcatc                                               <  2:3118019/150‑1 (MQ=255)
                                                                                                       gACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcacc                                               <  1:3373942/150‑3 (MQ=255)
                                                                                                         ccAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcatca                                              >  1:439228/1‑150 (MQ=255)
                                                                                                         ccAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcatca                                              >  2:15173/1‑150 (MQ=255)
                                                                                                          cAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAg                                             <  1:3954753/150‑1 (MQ=255)
                                                                                                               cgGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCAt                                        <  2:3769583/150‑1 (MQ=255)
                                                                                                                     cAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtgg                                  <  2:1413862/150‑1 (MQ=255)
                                                                                                                        gCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggtgg                               <  1:661660/150‑1 (MQ=255)
                                                                                                                        gCTTACGCACAAAATTCGGAGTATGGGTGCCTTTTTTGACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggtgg                               <  2:4119100/150‑1 (MQ=255)
                                                                                                                         cTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTggtggt                              <  2:461888/150‑1 (MQ=255)
                                                                                                                         cTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTggtggt                              <  2:137288/150‑1 (MQ=255)
                                                                                                                         cTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTggtggt                              <  2:2944164/150‑1 (MQ=255)
                                                                                                                          ttACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGgtggtg                             >  2:648399/1‑150 (MQ=255)
                                                                                                                              gCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATaa                         <  1:1095666/150‑1 (MQ=255)
                                                                                                                              gCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATaa                         <  1:1968419/150‑1 (MQ=255)
                                                                                                                                acaAAATTCTGATTATGGGTGCCTTTTTTTACCAGATTAAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGc                       >  2:2716593/1‑150 (MQ=255)
                                                                                                                                acaAAATTCGGATTATGGGTGCCTTTTTGTACCAGCTTCAGCCACGGACCTTCCAGATGCAGACCTACCGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGc                       <  2:4064246/150‑1 (MQ=255)
                                                                                                                                  aaaaTTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGt                     >  1:1063890/1‑150 (MQ=255)
                                                                                                                                  aaaaTTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGt                     >  1:3774930/1‑150 (MQ=255)
                                                                                                                                   aaaTTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTc                    <  1:1193496/150‑1 (MQ=255)
                                                                                                                                   aaaTTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTc                    <  1:1167758/150‑1 (MQ=255)
                                                                                                                                     aTTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCgg                  >  2:1986610/1‑150 (MQ=255)
                                                                                                                                       tCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCa                <  1:2518492/150‑1 (MQ=255)
                                                                                                                                       tCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCa                <  1:2070547/150‑1 (MQ=255)
                                                                                                                                         ggATTATGGGTGCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAATCGTCGGCAGAt             >  2:3587308/1‑150 (MQ=255)
                                                                                                                                         ggATTATGGGTGCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGAt             >  2:4028758/1‑150 (MQ=255)
                                                                                                                                         ggATTATGGGTGCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGAt             <  1:3297829/150‑1 (MQ=255)
                                                                                                                                            ttATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGAtag           >  1:973707/1‑150 (MQ=255)
                                                                                                                                                  gTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTa     >  2:3078524/1‑150 (MQ=255)
                                                                                                                                                     ccTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAg  <  1:1518001/150‑1 (MQ=255)
                                                                                                                                                     ccTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAg  <  2:1941500/150‑1 (MQ=255)
                                                                                                                                                     ccTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAg  <  1:2638044/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAG  >  NC_000913/702181‑702479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: