Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 361,323 G→C 13.3% R180G (CGG→GGG)  lacA ← thiogalactoside acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913361,3230GC13.3% 742.3 / 23.2 260R180G (CGG→GGG) lacAthiogalactoside acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (88/132);  new base C (37/2);  total (125/135)
Fisher's exact test for biased strand distribution p-value = 3.70e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

GCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCGGAA  >  NC_000913/361176‑361472
                                                                                                                                                   |                                                                                                                                                     
 ccGGATGCGGCTAGTGTCGATCGCTGATCTTGTAGGCCTGATAAGCGCTGCGTATCAGGCGATTTTTATAATTTAAACTGACGATTCAACTTTATTATCTTTGAAAAAATAGTGCTTATCCCGGTCGTGTATTTCGAGAAAAAACAGAcc                                                                                                                                                    >  2:1480408/1‑149 (MQ=255)
 ccGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTCGCGCATAACCCGAcg                                                                                                                                                    >  2:1199667/1‑149 (MQ=255)
  cGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACaa                                                                                                                                                   <  2:1354102/150‑1 (MQ=255)
  cGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGAGAATAAACCGACaa                                                                                                                                                   >  2:3689368/1‑150 (MQ=255)
  cGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTGTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACaa                                                                                                                                                   <  2:2157354/150‑1 (MQ=255)
     aTGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGa                                                                                                                                                <  2:2204905/150‑1 (MQ=255)
      tGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGaa                                                                                                                                               <  2:3398068/150‑1 (MQ=255)
      tGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGaa                                                                                                                                               <  1:1641347/150‑1 (MQ=255)
      tGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGaa                                                                                                                                               <  1:3156672/150‑1 (MQ=255)
        cGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAACg                                                                                                                                             >  1:148057/1‑150 (MQ=255)
          gatAATGTAGATCGCTGATCTTGTAGGCCTGATAAGCGCTGCTTATCAGTCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATTGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGcc                                                                                                                                           <  1:106786/148‑1 (MQ=255)
          gCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGAAAAGGAACGcc                                                                                                                                           >  2:885507/1‑150 (MQ=255)
              aTGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAAcgcccgcc                                                                                                                                       >  2:3355358/1‑150 (MQ=255)
               tGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAgccg                                                                                                                                      >  2:4114057/1‑150 (MQ=255)
                  tgATCGCTTAAGTTGTAGGCCTGATAAGCGCAGCGTATCAGGAAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCTAAAAACCCGACAAGGAACGCCAGCCGCCa                                                                                                                                   <  1:2578877/149‑1 (MQ=255)
                   gATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAACCCCAGCCGCCac                                                                                                                                  >  2:1500313/1‑150 (MQ=255)
                    aTCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAAAGCCAGCCGCCacc                                                                                                                                 >  1:2831470/1‑149 (MQ=255)
                    aTCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGAGCTTATCCCGGTCGTTTAATTAGCCAATAACCCGACCAGGCACGCCAGCCGCCacg                                                                                                                                 >  2:3299262/1‑150 (MQ=39)
                        cTGACCTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGGTTATTTCGCGAAGAACCCGCCAAGGTACGCGAGCCGCCacgacgg                                                                                                                             >  2:2282609/1‑149 (MQ=255)
                        cTGAACTTGTAGGCCTGATACGCGCAGCGTATCTGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATTGTGCTTATCCGGGTCGTTTATTTCGCGAATACCCCGACAAGGAACGCCAGCCGCCACGACGt                                                                                                                             <  1:1508002/150‑1 (MQ=255)
                         tGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGGCAATGAACGCCAGCCGCCACGACGtt                                                                                                                            >  1:1349486/1‑150 (MQ=255)
                         tGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAACCCGACAACGCACGCCCGCCGCCaccacggt                                                                                                                            >  2:1600613/1‑148 (MQ=39)
                           aaCTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCCACAAGGAACGCCCGCCCCCACGACGGTtg                                                                                                                          >  1:3725012/1‑150 (MQ=39)
                            aCTTGTTGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTAAAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtgg                                                                                                                         <  1:1026841/150‑1 (MQ=255)
                            aCTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGTCGATCCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtgg                                                                                                                         <  2:3500237/150‑1 (MQ=255)
                             cTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtggt                                                                                                                        <  2:4049207/150‑1 (MQ=255)
                             cTTGTAGGCCTGATAAGCGCAGCGTATCAGGAAATTTTTATAATTTAAACTGACGATTCAACTTTATAATATTTGAAATAATAGTGCTTATCCCGGTCTTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtggt                                                                                                                        <  1:1765245/150‑1 (MQ=255)
                              ttGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCACCACCGTtggtg                                                                                                                       >  2:2312199/1‑150 (MQ=255)
                              ttGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTCGCGAAAAACCCGACAAGGAACGCCACCCGCCCCGACGGTttgtg                                                                                                                       >  2:2844601/1‑150 (MQ=255)
                                 tAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGGATAAACCGACAAGGAACGCCCGCCCCCACCACGTTtggtggga                                                                                                                    >  1:3912366/1‑148 (MQ=255)
                                  aGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAAt                                                                                                                   <  1:188926/150‑1 (MQ=255)
                                  aGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAAt                                                                                                                   <  1:3681014/150‑1 (MQ=255)
                                    gCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGAATAACCCGACCAGGAACGCCCGCCCCCACCACGGTTGGGGGGATGt                                                                                                                 >  1:3832740/1‑150 (MQ=255)
                                    gCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTCGCGTATAACCCGACAAGGAACGCCCGCCGCCACGCCGTTTGGTGGAATGt                                                                                                                 >  2:1667110/1‑150 (MQ=255)
                                      cTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCt                                                                                                               <  1:2357984/150‑1 (MQ=255)
                                      cTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGCATAAACCCACAAGGAACCCCACCCCCCCCGACGTTTGGTGGAATGTCt                                                                                                               >  1:1859035/1‑150 (MQ=255)
                                        gATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCttt                                                                                                             <  2:482258/150‑1 (MQ=255)
                                         aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtttt                                                                                                            <  2:4019859/150‑1 (MQ=255)
                                         aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtttt                                                                                                            <  2:1868400/150‑1 (MQ=255)
                                         aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTGGCGAAAAAAAACACAAGGTACCCCCGCCGCCACCACCGTTGGGGGACTGTCtttt                                                                                                            >  2:712620/1‑150 (MQ=255)
                                          tAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAACACGCCCAGGAACGCCAGCCGCCACGCCGGTTGGTGGAATGTCTTTtg                                                                                                           >  2:1952423/1‑150 (MQ=255)
                                            aGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTtgtg                                                                                                         <  2:2691191/150‑1 (MQ=255)
                                             gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                                        <  1:3363112/150‑1 (MQ=255)
                                             gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                                        <  2:2789182/150‑1 (MQ=255)
                                              cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGAc                                                                                                       <  2:785162/150‑1 (MQ=255)
                                              cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGAc                                                                                                       <  2:514695/150‑1 (MQ=255)
                                              cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGAATGTCtttttttac                                                                                                       >  2:3207882/1‑145 (MQ=255)
                                              cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCCACAAGGAACGCCAGCCGCCACGACGTTTTGTGGAATGTCTTTTGTGAc                                                                                                       >  2:2052257/1‑150 (MQ=255)
                                              cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGAATCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGGGTAGCCCCACAAGGACCCGCCGCCCCCCCGAGGGTTGGTGGGATGTGGTGTGTGag                                                                                                       >  1:2494643/1‑149 (MQ=255)
                                               gcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAAGAACCCCAGCCCCCACCACGGTTGGTGGGAGGTGTTTTTTGACg                                                                                                      >  1:3927210/1‑150 (MQ=255)
                                                      aTCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGAtactac                                                                                               <  1:2092849/150‑1 (MQ=255)
                                                         aGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAACCCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGAAAGTCTTTTGTGACGATACTACCcg                                                                                            >  2:933320/1‑150 (MQ=255)
                                                          ggCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCcgc                                                                                           <  2:1785788/150‑1 (MQ=255)
                                                          ggCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTAACcgc                                                                                           >  2:975924/1‑150 (MQ=255)
                                                           gCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAACACGACAAGGAACGCCCGCCGCCACGACGGTTTGTGGTGTGTGTTTTGTGTAGATAATAACcgcg                                                                                          >  1:67167/1‑150 (MQ=255)
                                                            cAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGAGAATAAACCGACAAAGAACCCCCGCCCCCCCCACCGTGTGTGGGATGTGTTTTGTGTCGATACGAACCccgc                                                                                         >  1:481161/1‑150 (MQ=255)
                                                             aaTTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAAACCACAAGGAAACCCACCCCCCACCACGTTTTGTGGGATGTCTTTTTTTTCGATAATAACCCCGcc                                                                                        >  1:1990417/1‑150 (MQ=255)
                                                             aaTTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATTACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGGATGTCTTTTTTGACGATACTAACCGCGcc                                                                                        >  2:3022092/1‑150 (MQ=255)
                                                                ttttATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAAt                                                                                     <  1:1084007/150‑1 (MQ=255)
                                                                ttttATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAAt                                                                                     <  2:1778615/150‑1 (MQ=255)
                                                                   tataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAAc                                                                                  <  1:3022055/150‑1 (MQ=255)
                                                                   tataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAAAGCCAGCCGCCACCACCTTTGGTGGAAATTATTTTGTGACGATACAACCCGCGCCAaaaaa                                                                                  >  2:448840/1‑149 (MQ=255)
                                                                    ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACa                                                                                 <  1:3458610/150‑1 (MQ=255)
                                                                    ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACa                                                                                 <  1:3277585/150‑1 (MQ=255)
                                                                    ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACa                                                                                 <  1:3420433/150‑1 (MQ=255)
                                                                    ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACa                                                                                 <  1:3673565/150‑1 (MQ=255)
                                                                       aTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGaa                                                                              <  1:411075/150‑1 (MQ=255)
                                                                              cTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATccc                                                                       <  2:2927309/150‑1 (MQ=255)
                                                                                 aCGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGa                                                                    <  1:3456119/150‑1 (MQ=255)
                                                                                 aCGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGa                                                                    <  1:43521/150‑1 (MQ=255)
                                                                                    aTTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGAATAAAACCACAAAGAACCCCCCCCCCCACCACGGTTGGGGGAATGTAGTTTTTTTAGATAATAACCGCCCCAAAAAAAAGATTATacccccagg                                                                 >  1:3074846/1‑145 (MQ=255)
                                                                                             tataATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTg                                                        <  1:3318788/150‑1 (MQ=255)
                                                                                             tataATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGACTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTg                                                        <  2:4090290/150‑1 (MQ=255)
                                                                                                    tttGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAAAGTCTTTTGTGACGATACTACCCCCGCCAAAAACAGAATTATCCCCGATGGTGACGCCGGGATtaat                                                 >  2:1704609/1‑150 (MQ=255)
                                                                                                      tGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaataa                                               <  1:2258321/150‑1 (MQ=255)
                                                                                                         aataatAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACca                                            <  2:3645752/150‑1 (MQ=255)
                                                                                                          ataataGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACcac                                           <  1:1137068/150‑1 (MQ=255)
                                                                                                            aataGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACAt                                         <  1:916563/150‑1 (MQ=255)
                                                                                                               aGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGAc                                      <  2:1280148/150‑1 (MQ=255)
                                                                                                                gTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAACGAACGCCAGCCGCCACGACGTTTGGAGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACACAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACt                                     <  1:524297/150‑1 (MQ=255)
                                                                                                                 tGCTTATCCCGGTCGTTTATTTAGCGAATAACCCCACAAGGAACGCCAGCCGCCACCACGGTTGGTGGAATGTCGTTTGTTTCGATACTACCCCCGCCAAAAACAGAATTATCCCCGAGGGTGACGCCTGGATTAATAAACACAAGaaat                                    >  2:496894/1‑147 (MQ=255)
                                                                                                                   cTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAAAGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGTCGATACTACCCGCGCCAAAAACAGAAATATACCCCATTGTGACGCCTGGATTAATAACCACAAGACTTcc                                  >  2:1376787/1‑150 (MQ=255)
                                                                                                                    ttATCCCGGTCGTTTATTTCGCGAATAACCCCACAAGGAACGCCAGCCGCCACGACGTTTGGGGGAATGTCTTTTGTGACGATACTACCCCCGCCAATAACAGAAATATACCCCATGGTGACGCCGGGCTTAATAACCACATGACATCCg                                 >  2:2503385/1‑150 (MQ=255)
                                                                                                                    ttATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCg                                 >  2:665780/1‑150 (MQ=255)
                                                                                                                    gtATCCCGGTCGTTTATTTCGCGAAGAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCg                                 <  1:3546724/149‑1 (MQ=255)
                                                                                                                     tATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATCACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGa                                <  1:2126375/150‑1 (MQ=255)
                                                                                                                     tATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGa                                <  2:312210/150‑1 (MQ=255)
                                                                                                                     tATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGa                                <  2:300322/150‑1 (MQ=255)
                                                                                                                       tCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATc                              <  2:3074846/150‑1 (MQ=255)
                                                                                                                           ggTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACAAACCGCGCCAATAAAAGAATTATACCCGATGGTGACGCCTGGATTAATAAACACAAGACATCCGATCCAGa                          >  2:3797144/1‑150 (MQ=255)
                                                                                                                               gTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGtt                      >  2:2473790/1‑150 (MQ=255)
                                                                                                                                tttATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTa                     >  1:269985/1‑150 (MQ=255)
                                                                                                                                   aTTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTg                  <  1:1561082/150‑1 (MQ=255)
                                                                                                                                     ttCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGcc                <  2:2403525/150‑1 (MQ=255)
                                                                                                                                      tCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCa               >  1:2560626/1‑150 (MQ=255)
                                                                                                                                       cgcgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCaa              >  2:3908433/1‑150 (MQ=255)
                                                                                                                                         cgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATc            <  1:3207882/150‑1 (MQ=255)
                                                                                                                                                 ccGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCgg    <  2:1075656/150‑1 (MQ=255)
                                                                                                                                                 ccGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCgg    >  1:3913424/1‑150 (MQ=255)
                                                                                                                                                  cGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCGGa   <  2:1969214/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCGGAA  >  NC_000913/361176‑361472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: