Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,554,283 T→A 5.4% L400Q (CTG→CAG)  malT → mal regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,554,2830TA 5.4% 941.2 / 2.5 310L400Q (CTG→CAG) malTmal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (119/162);  new base A (25/2);  total (146/164)
Fisher's exact test for biased strand distribution p-value = 2.20e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

GATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAG  >  NC_000913/3554137‑3554432
                                                                                                                                                  |                                                                                                                                                     
gATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGcc                                                                                                                                                    >  2:2391115/1‑150 (MQ=255)
   ggCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTg                                                                                                                                                 <  1:2099942/150‑1 (MQ=255)
   ggCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTg                                                                                                                                                 <  2:631082/150‑1 (MQ=255)
     cccAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTggg                                                                                                                                               >  2:2486087/1‑150 (MQ=255)
     cccAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTggg                                                                                                                                               >  2:3139228/1‑150 (MQ=255)
      ccAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGa                                                                                                                                              <  1:3903989/150‑1 (MQ=255)
      ccAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGa                                                                                                                                              <  1:3444726/150‑1 (MQ=255)
       cAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGAc                                                                                                                                             >  1:4081953/1‑150 (MQ=255)
        aGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACa                                                                                                                                            <  2:4036196/150‑1 (MQ=255)
             tttCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTg                                                                                                                                       <  1:2404586/150‑1 (MQ=255)
              ttCCCAGCGAATCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGc                                                                                                                                      <  2:2546770/150‑1 (MQ=255)
                cccAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTg                                                                                                                                    >  1:2184821/1‑150 (MQ=255)
                  cAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGa                                                                                                                                  <  2:2461556/150‑1 (MQ=255)
                  cAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGa                                                                                                                                  >  2:3397600/1‑150 (MQ=255)
                   aGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGaa                                                                                                                                 >  1:555827/1‑150 (MQ=255)
                   aGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGaa                                                                                                                                 <  2:344693/150‑1 (MQ=255)
                      gAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGCCAGCTTGCTGGAAAAt                                                                                                                              >  1:434616/1‑150 (MQ=255)
                        aGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATcc                                                                                                                            >  1:2714578/1‑150 (MQ=255)
                          cAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGc                                                                                                                          <  1:735515/150‑1 (MQ=255)
                           aaTTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCa                                                                                                                         >  1:2672346/1‑150 (MQ=255)
                           aaTTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCa                                                                                                                         >  1:2700466/1‑150 (MQ=255)
                              tcatcaTGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGtt                                                                                                                      <  1:1635585/150‑1 (MQ=255)
                                atcatGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTgg                                                                                                                    <  1:1363539/150‑1 (MQ=255)
                                atcatGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTgg                                                                                                                    <  2:1424383/150‑1 (MQ=255)
                                 tcatGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCGTGCTGGAAAATCCGCAGTTGgt                                                                                                                   >  2:669933/1‑150 (MQ=255)
                                   atGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGgtgt                                                                                                                 <  2:1209619/150‑1 (MQ=255)
                                     gcgcTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTa                                                                                                               >  1:927645/1‑150 (MQ=255)
                                      cgcTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTAt                                                                                                              <  1:4043194/150‑1 (MQ=255)
                                       gcTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCAGCCGTGGGACAGCTTGCTTGAAAATACGCAGTTTGTGTTAtt                                                                                                             >  1:1782802/1‑150 (MQ=255)
                                        cTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTg                                                                                                            <  1:1311167/150‑1 (MQ=255)
                                         tGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGc                                                                                                           <  1:1249009/150‑1 (MQ=255)
                                         tGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGc                                                                                                           <  2:2828659/150‑1 (MQ=255)
                                          ggcggcAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCa                                                                                                          <  2:1242258/150‑1 (MQ=255)
                                          ggcggcAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCa                                                                                                          <  2:1107092/150‑1 (MQ=255)
                                          ggcggcAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCa                                                                                                          <  2:3501194/150‑1 (MQ=255)
                                               cAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGt                                                                                                     <  2:2192519/150‑1 (MQ=255)
                                                  gCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGc                                                                                                  <  2:1691991/150‑1 (MQ=255)
                                                     aTGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGa                                                                                               <  1:1934000/150‑1 (MQ=255)
                                                     aTGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGa                                                                                               >  1:2321073/1‑150 (MQ=255)
                                                     aTGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCGTGCTGGAAAATACGCAGTTGGTGTTATTGCAGGCGTGGCTGa                                                                                               >  1:792601/1‑150 (MQ=255)
                                                     aTGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCGTAAGGCCCAGCCCGGGGACACCTTGCGGGAAAATCCCCACGTGGTGTTATTGCAGGCGTGGCGGa                                                                                               >  2:1857823/1‑150 (MQ=255)
                                                       gcgcTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATg                                                                                             <  2:1517806/150‑1 (MQ=255)
                                                        cgcTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCCGTTGGTGTTATTGCAGGCGTGGCTGATGc                                                                                            >  2:3668659/1‑150 (MQ=255)
                                                           tGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCaaa                                                                                         <  1:96341/150‑1 (MQ=255)
                                                           tGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCAGAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCaaa                                                                                         <  1:3864459/150‑1 (MQ=255)
                                                            gATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAg                                                                                        <  2:173515/150‑1 (MQ=255)
                                                              tgctgcGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGcc                                                                                      <  1:3051353/150‑1 (MQ=255)
                                                                ctgcGCGATATTCTACTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCaa                                                                                    >  1:659424/1‑150 (MQ=255)
                                                                    gcgATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCCACATc                                                                                >  2:3951215/1‑150 (MQ=255)
                                                                    gcgATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATc                                                                                >  2:3325643/1‑150 (MQ=255)
                                                                    gcgATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATc                                                                                >  2:2236194/1‑150 (MQ=255)
                                                                            cTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAATGCCCAGCCCTGGGACAGCTTGCTGGAAAAAACGCAGTTGGTGTTATTGCAGGCGTGGCTGATTCAAAGCCAACATAGCTaacgc                                                                        >  2:2952114/1‑146 (MQ=255)
                                                                                ttAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACCGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCCACATCGCTACGGCGAAg                                                                    >  2:2789665/1‑150 (MQ=255)
                                                                                ttAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAg                                                                    >  2:4076351/1‑150 (MQ=255)
                                                                                  aaTCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATAGCTACGGCGAAGtt                                                                  >  2:895073/1‑150 (MQ=255)
                                                                                    tCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTaa                                                                <  2:156824/150‑1 (MQ=255)
                                                                                     cACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAac                                                               <  2:843718/150‑1 (MQ=255)
                                                                                      aCGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTTAGGCCCAGCCGTGGGGCAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTTCAGGCGTGGCTGATGCAAAGCCAACATAGCAACGGCGAAGTTAaca                                                              >  1:1562036/1‑150 (MQ=255)
                                                                                      aCGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAaca                                                              <  2:3860666/150‑1 (MQ=255)
                                                                                      aCGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCAGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAaca                                                              >  2:3062420/1‑150 (MQ=255)
                                                                                        gCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACAcc                                                            <  2:555827/150‑1 (MQ=255)
                                                                                        gCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCAGCCGTGGGACAGCGTGCTGGAAAATCCGCAGTTGGTGTTATTTCAGGCGTGGCGGATGCAAAGCCAACAAAGCTACCGCGAAGTTAAAAcc                                                            >  1:3606534/1‑150 (MQ=255)
                                                                                        gCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCGTTAGGCCCTGCCGTGGGGCAGCGTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGTTGCAAAGCCAAAAAAGCAACGGCGAAGTTAACAcc                                                            >  1:1158259/1‑150 (MQ=255)
                                                                                           tGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTg                                                         <  2:949823/150‑1 (MQ=255)
                                                                                             gAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCt                                                       >  2:3758059/1‑150 (MQ=255)
                                                                                                tCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCCAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGc                                                    >  2:1899905/1‑150 (MQ=255)
                                                                                                  tGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTTGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGccc                                                  >  2:3372854/1‑150 (MQ=255)
                                                                                                  tGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCGTAAGGCCCAGCCCGGGGCCACCGTGCTGGCAAATCCCCAGTTGGTGGTATTGCAGGCGTGGCTGATGCCAACCCAACAACGCGACGGCGCAGGTAACACCCAGCTAGccc                                                  >  1:2951876/1‑150 (MQ=255)
                                                                                                    ttCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGt                                                >  2:3605389/1‑150 (MQ=255)
                                                                                                    ttCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGt                                                <  2:2711026/150‑1 (MQ=255)
                                                                                                    ttCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATACGCAGTTGGTGTTATTTCAGGCGTGGCTGATTCAAAGCCAAAATCCCTACGGCGAAGTTAACACCCAGCTAGcccct                                                >  1:2288208/1‑148 (MQ=255)
                                                                                                    ttCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCAGCCGTGGGACAGCTTGCTGGAAAAACCCCAGTTGGTGTTATTTCAGGCGTGGCTGATTCAAAGCCCACATCGCTACGGCGAAGTTAACACCCAGCAAGCCCgg                                                >  2:1781279/1‑149 (MQ=255)
                                                                                                      cAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGc                                              <  2:823207/150‑1 (MQ=255)
                                                                                                          cATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGaa                                          <  2:2148621/150‑1 (MQ=255)
                                                                                                          cATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGaa                                          <  2:3807195/150‑1 (MQ=255)
                                                                                                          cATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGaa                                          >  2:4106372/1‑150 (MQ=255)
                                                                                                          cATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCAGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCACAACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGaa                                          >  2:3257131/1‑150 (MQ=255)
                                                                                                           aTAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAAc                                         >  2:2328448/1‑150 (MQ=255)
                                                                                                           aTAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAAc                                         >  2:2643891/1‑150 (MQ=255)
                                                                                                               cGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGa                                     <  2:3109125/150‑1 (MQ=255)
                                                                                                                gAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGaa                                    <  2:1136990/150‑1 (MQ=255)
                                                                                                                 aaCTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCCCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACAACGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGaaa                                   >  2:2808485/1‑150 (MQ=255)
                                                                                                                     gTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCaa                               <  1:3139228/150‑1 (MQ=255)
                                                                                                                     gTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCaa                               <  1:3748931/150‑1 (MQ=255)
                                                                                                                      tCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAg                              <  2:753436/150‑1 (MQ=255)
                                                                                                                         ctgctgGAAGAGTCGCTTAAGCACCAGCCCAGGGACACCATGCTGGAAAATCCCCATTTGGTGTTATATCAGGCGTGGCTGATGCAAAGCCAAAATACCTACGGCCAAGTTAACACCCTGCTAGCCCGTGCTGAACAACAAAACAAGGAc                           >  2:1946497/1‑150 (MQ=255)
                                                                                                                             tgGAAGAGTCGCTTAAGGCCCTGCCGTTGGACAGCTTGCTGGAAAATCCGCAGGTGGTGTTATTGCATGCGTGGCGGATGCAAAGCCAACAACGCTACGGCGAAGTTAACACCCTGCAAGCCCGTGCTGAACATGAAATCAAGGtcatca                       >  2:3582467/1‑150 (MQ=255)
                                                                                                                             tgGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCa                       <  1:2453433/150‑1 (MQ=255)
                                                                                                                                   agTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGagaag                 >  2:2268132/1‑150 (MQ=255)
                                                                                                                                       gCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGacac             >  2:425115/1‑150 (MQ=255)
                                                                                                                                       gCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGacac             >  2:2010508/1‑150 (MQ=255)
                                                                                                                                        cTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACAcc            >  1:4109408/1‑150 (MQ=255)
                                                                                                                                         ttAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGATGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCa           <  1:249845/150‑1 (MQ=255)
                                                                                                                                             ggCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCa       <  1:1657771/150‑1 (MQ=255)
                                                                                                                                              gCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCATAGAAGACACCATGCAc      >  1:3815617/1‑150 (MQ=255)
                                                                                                                                              gCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCAc      >  1:3233539/1‑150 (MQ=255)
                                                                                                                                              gCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCAc      >  1:3434232/1‑150 (MQ=255)
                                                                                                                                                ccTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCACAGCCAACCTCGCTCCGGCGCAGTTAACACCCTGCTCGCCCGTGCTGAACATGAACTCAAGGACATCAGAGAAGACACCATGCACGc    >  2:4015671/1‑150 (MQ=255)
                                                                                                                                                ccTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGc    >  1:3306530/1‑150 (MQ=255)
                                                                                                                                                ccTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGc    >  1:1013204/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCCGTGGGACAGCTTGCTGGAAAATCCGCAGTTGGTGTTATTGCAGGCGTGGCTGATGCAAAGCCAACATCGCTACGGCGAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAG  >  NC_000913/3554137‑3554432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: