Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,695 C→G 8.2% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,6950CG 8.2% 670.5 / 15.2 208intergenic (+46/+56)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (93/95);  new base G (2/18);  total (95/113)
Fisher's exact test for biased strand distribution p-value = 6.39e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATA  >  NC_000913/3608546‑3608844
                                                                                                                                                     |                                                                                                                                                     
ccacGCTGTTAGGGATGACGGGGTTGGGGCTGGCAGTGCTGGCAGATCCGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAATATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCctc                                                                                                                                                       <  1:1796021/150‑1 (MQ=255)
ccacGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCctc                                                                                                                                                       >  2:757048/1‑150 (MQ=255)
  acGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTg                                                                                                                                                     >  1:431668/1‑150 (MQ=255)
   ggCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                    <  1:2403099/149‑1 (MQ=255)
   cGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCTGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                    <  1:3894073/150‑1 (MQ=255)
   cGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                    <  2:1452592/150‑1 (MQ=255)
   cGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                    <  1:16787/150‑1 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCATATACGGGGGCGACGGTGCTGGTGACAGCTAATGCGTTAAGATTGTTGCGCAGTATATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   <  1:3659820/150‑1 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   <  2:3121476/150‑1 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   >  1:2484508/1‑150 (MQ=255)
         tAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                              >  2:793276/1‑150 (MQ=255)
         tAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                              <  2:1013938/150‑1 (MQ=255)
           gggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                                                                            <  1:212944/150‑1 (MQ=255)
            ggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                           >  2:1293731/1‑150 (MQ=255)
             gATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCTCCTCTCCCCAGAGGGGCGAgggga                                                                                                                                          <  2:660292/150‑3 (MQ=255)
             gATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGtt                                                                                                                                          >  2:2899631/1‑150 (MQ=255)
             gATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGATAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCTCCCCAGAGGGGCGAgggga                                                                                                                                          <  1:2973871/150‑3 (MQ=255)
               tGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                                                                                        >  2:3909125/1‑150 (MQ=255)
               agACCGGGTGGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                                                                                        <  2:3970279/149‑1 (MQ=255)
                    gggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa                                                                                                                                   >  1:1869167/1‑150 (MQ=255)
                    gggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa                                                                                                                                   <  2:3771392/150‑1 (MQ=255)
                     ggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGCCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                                  <  1:54342/150‑1 (MQ=255)
                      gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                 <  1:3472709/150‑1 (MQ=255)
                        tgtgGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACAGTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                                                                                               >  2:2800191/1‑150 (MQ=255)
                           ggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                            >  1:1502750/1‑150 (MQ=255)
                            gctggcCGTGCTGGCAGATCCGGGGGCGACGGCGCTGGTGACAGCGCCTGCGTTAAGCTTGTTGCGCCGGAGACAAGCCACACCGCTCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCCCTGGGGAGAGGGTTAGGGTGAGGGGaaaa                                                                                                                           <  1:1027385/150‑1 (MQ=37)
                              tggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAGACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                         >  2:3757367/1‑150 (MQ=255)
                                gctgTGCTGGCTGATTGGGGGGCGACGGTGCTGGTGACTGCGATTGCGTTAAGTTTGTTGCGCAGGAGATAAGGCAACCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGCGGGGAAAAggcg                                                                                                                       <  2:3318189/147‑1 (MQ=38)
                                   gTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGAGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCa                                                                                                                    >  2:182632/1‑150 (MQ=255)
                                    tGCTGGCAGTTAGGGGGGCGACGGTGCTGGTGACAGCGAATGCTTTATGATTGTTGGGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATGGGTCCCCTCGCCCCTCTGGGGTGTGGTTTTGGTTGAGGGGAAAAGGCGGCAt                                                                                                                   <  2:3438177/150‑1 (MQ=255)
                                     gcgggCAGATAGGGGGCGGCCGGTGCTGGTGACCGGGATTGCGTTAAGTTTGTGGCCCTGGAGTTACGGCAAACCGCTCGCAACTTTGCGCGCGATCGGTCCCCTCGCCCCTGTGGGGTGTGGGTTCGGGTGTGGGGAAAAGGCGGCATc                                                                                                                  <  2:461051/147‑1 (MQ=255)
                                        ggCCGATCCGGGGGCGCGGGTGGTGGTGACAGCGTTTGGTTTAATTTTTTTGCGCTGGATTTACGGCAACCCGATCGCAAAATTGAGCGGGATGGGTCCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTGAGGGGAAAAGGCGGCATCGaa                                                                                                               <  2:2499455/150‑1 (MQ=16)
                                         gCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAg                                                                                                              >  1:3177541/1‑150 (MQ=255)
                                         gCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAg                                                                                                              >  1:878847/1‑150 (MQ=255)
                                         gCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGAGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAg                                                                                                              >  1:211132/1‑150 (MQ=255)
                                                     gCGACGGTGCTGGTGACAGCGAATGCTTTAATATTGTTGCGCAGGAGATAAGGCAAACCTATCGCAACATTGAGCGCTATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGcccc                                                                                                  <  2:603845/150‑1 (MQ=255)
                                                      cGACGTGGCGGGTGACCGCGAATGGGTTAAGTTTGTTGCGCTGGAGATACGGCAACCCTATCGCAACATTTAGCGCGATGGGTCCCCTCGCCCCGCGGGGTAGCGGTTTTGGGTGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCt                                                                                                 <  2:1647988/150‑1 (MQ=255)
                                                          ggTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTGAGGGTGAGGGGAAAAGGCGGCATCGAAGCCCATCAGCCCCGATCa                                                                                             >  2:2553540/1‑150 (MQ=255)
                                                             gCTGGTGACAGCGATTGCGTTATGATTGGTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGCGCGCGAGCGGTCCCCTCGCCCCTGTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAAcc                                                                                          <  2:2743867/150‑1 (MQ=38)
                                                                  tGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                                                     >  1:2337837/1‑150 (MQ=255)
                                                                   gACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCttt                                                                                    >  2:1946605/1‑150 (MQ=255)
                                                                    aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                   >  1:2329834/1‑150 (MQ=255)
                                                                    aCAGCGAATGCGATAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                   >  1:290119/1‑150 (MQ=255)
                                                                     cAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTAc                                                                                  >  1:3991057/1‑150 (MQ=255)
                                                                        cgatcgCGTTAAGATGGTTGCGCAGGAGATAAGGCAACCCGATCGCAACATTGCGCGCGATCGGTCCCCTCGCCCCGCGGGGGAGAGGGTTAGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGaa                                                                               <  2:2615627/145‑1 (MQ=38)
                                                                        cGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGaa                                                                               <  2:3753200/150‑1 (MQ=255)
                                                                        cGAAGGCTTTAAGTTGGTTGCCCTGGCGATAAGGCAAACCTATCGAAAAATTGACCGCGATCGGTCCCCTCGCCCCTCTGGGTGGTGGGTTTGGTTTTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGaa                                                                               <  2:574513/150‑1 (MQ=255)
                                                                           aTGCGTTAAGATTGTGGCGAAGGACATAAGGCAAACCGACCGCAACATTGCGCGCGATCGGGCCCGTCGCCCCTCTGGGGAGTGGGTTTGGTTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCa                                                                            <  1:4018166/150‑1 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  2:2491969/1‑150 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  2:685014/1‑150 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  1:802669/1‑150 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  2:1331461/1‑150 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  2:531807/1‑150 (MQ=255)
                                                                                ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                       >  1:1120273/1‑150 (MQ=255)
                                                                                ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                       >  1:845512/1‑150 (MQ=255)
                                                                                   aGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGa                                                                    >  1:354930/1‑150 (MQ=255)
                                                                                     aTTGTTGCGCAGGAGATACGGCAACCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATa                                                                  <  2:3108215/150‑1 (MQ=255)
                                                                                      ttggtgCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                 <  2:4125043/150‑1 (MQ=255)
                                                                                      ggggtgCGCCGGAGATACGGCAAACCGACCGCAACATTGACCGCGATCGGGCCCCTCCCCCCTCGGGGGCGTGGGTCAGGGGGAGGGGAAAAGGCGGCATCGACGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                 <  2:3055189/146‑1 (MQ=255)
                                                                                         ttgCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGc                                                              >  2:3889031/1‑150 (MQ=255)
                                                                                          tgCGCTGGAGTTTCGGCAACCCTATCGCAACTTTGTGCGCGTTCGGGCCCCTCGCCCGTGTGGGGTGTGGTTTTGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCa                                                             <  1:2261358/150‑1 (MQ=255)
                                                                                          tgCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCa                                                             >  1:1557565/1‑150 (MQ=255)
                                                                                               aGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCa                                                        >  1:597185/1‑150 (MQ=255)
                                                                                                      ccggCACACCTATCGAACCTTTGCGCGCGCTCGGGCCCCGCGCCCGTGGGGGGTGTGGGTGTGGGGGGGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtct                                                 <  1:2899631/148‑1 (MQ=255)
                                                                                                         gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                              >  1:1218370/1‑150 (MQ=255)
                                                                                                         gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGGCCATCCGTCTCtt                                              >  2:1142187/1‑150 (MQ=255)
                                                                                                          caccccGATCTCAACTTTGCGCGCTTTGGGCCCCCCCCCCCTTGTGGGTTGTGGGTTTGGTGGGGGGCACAAGGCGGTATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCttt                                             <  1:687316/146‑1 (MQ=255)
                                                                                                          cAACCCGATCGAAACTTTGCGCGCGATGGGTCCCCGCGCCCGGGGGGGGTGGGGTTTCGGGTGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCttt                                             <  1:1155632/150‑1 (MQ=255)
                                                                                                           aaaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCCTCCGTCTCTTTa                                            >  2:204846/1‑150 (MQ=255)
                                                                                                           aaaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                            >  1:3117418/1‑150 (MQ=255)
                                                                                                           aaaCCGATCGAAACATTGCGCGCGTGCGGCCCCCCCCCCCCCGTGGGGAGTGGGTTTGGGTGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                            <  1:1934151/150‑1 (MQ=255)
                                                                                                             aCCGATCGAACCATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGc                                          <  2:2096430/150‑1 (MQ=255)
                                                                                                              ccGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCa                                         >  1:2685289/1‑150 (MQ=255)
                                                                                                               ctaTCGCAACTTTGCGCGCGTTGGGGCCCCCCGCCCCTGTGGGTTGTGGGTTGGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                                        <  2:3529054/148‑1 (MQ=255)
                                                                                                               cGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGTGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                                        >  1:2740673/1‑150 (MQ=255)
                                                                                                                tctCGCAACATTGCGCGCGATCGGTCCCCGCGCCCCTCTGGGGTGAGGTTTTGGTGGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                       <  1:4126787/148‑1 (MQ=255)
                                                                                                                gATCGCAACATTGCGCGCGAGCGGCCCCCTCGCCCCTCGGGGGCGCGGGTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                       <  2:3607943/150‑1 (MQ=255)
                                                                                                                gATCGAAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGATAGTGTTAGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAACTAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                       <  2:2331847/150‑1 (MQ=255)
                                                                                                                 aTCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAcc                                      >  2:2928924/1‑150 (MQ=255)
                                                                                                                     caccATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                  >  2:2456748/4‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                  >  2:2456681/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                  >  2:2714457/1‑150 (MQ=255)
                                                                                                                                  gttCGGTCCCCTCGCCCCTCTGGGGTGTGGTTTCGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                     <  2:538066/148‑1 (MQ=255)
                                                                                                                                  gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                     >  1:302109/1‑150 (MQ=255)
                                                                                                                                   ttggttCCCCTCGCCCCTGTGGGGTGTGGTTTTGGGTGGGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                    <  2:1869167/145‑1 (MQ=255)
                                                                                                                                   aTCGGTCTCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                    <  2:2079531/150‑1 (MQ=255)
                                                                                                                                   aTCGGCCCCCTCGCCCCTCTGGGTAGTGGTTTTGGGTGTGGTGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                    <  1:263015/150‑1 (MQ=255)
                                                                                                                                     cGGTCCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGt                  <  2:2011803/150‑1 (MQ=255)
                                                                                                                                      ggTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTa                 >  1:195969/1‑150 (MQ=255)
                                                                                                                                        tCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACa               >  1:3416213/1‑150 (MQ=255)
                                                                                                                                         acccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAGAAAGGTACaa              >  2:4004968/2‑150 (MQ=255)
                                                                                                                                           ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaaa            <  1:3757367/150‑1 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAc           >  1:1345883/1‑150 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAc           >  1:1592990/1‑150 (MQ=255)
                                                                                                                                               gCCCCTCTGGGGAGAGGGTTCGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        <  2:1664904/150‑1 (MQ=255)
                                                                                                                                                ccccTCTGGGTAGTGGGTTAGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAg       <  2:3795062/150‑1 (MQ=255)
                                                                                                                                                ccccTCTGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAg       <  2:3728519/150‑1 (MQ=255)
                                                                                                                                                   ctctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGaa    >  1:2918693/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat   >  1:2580070/1‑150 (MQ=255)
                                                                                                                                                     ctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAata  >  1:3294921/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATA  >  NC_000913/3608546‑3608844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: