Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 543,091 G→C 5.3% intergenic (+58/+170) glxK → / ← allE glycerate kinase II/S‑ureidoglycine aminohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913543,0910GC 5.3% 803.7 / 28.3 222intergenic (+58/+170)glxK/allEglycerate kinase II/S‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand):  ref base G (98/110);  new base C (5/7);  total (103/119)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 229 total aligned reads displayed.

CCGAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAAT  >  NC_000913/542942‑543240
                                                                                                                                                     |                                                                                                                                                     
  gAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTCCCTTTAAAGCGGATGCGATTTATTTCGCATAAGAGTGCAGTACTCATGCCGGAt                                                                                                                                                     <  1:3581793/150‑1 (MQ=255)
   aaGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGACTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATg                                                                                                                                                    <  2:726941/150‑1 (MQ=255)
    aGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGc                                                                                                                                                   <  1:2379722/150‑1 (MQ=255)
    aGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGc                                                                                                                                                   <  1:2788557/150‑1 (MQ=255)
    aGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGc                                                                                                                                                   <  1:794364/150‑1 (MQ=255)
      tGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCgg                                                                                                                                                 <  1:751125/150‑1 (MQ=255)
      tGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCgg                                                                                                                                                 <  1:2922768/150‑1 (MQ=255)
      tGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCgg                                                                                                                                                 <  1:1967792/150‑1 (MQ=255)
      tGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCgg                                                                                                                                                 <  1:1157887/150‑1 (MQ=255)
        cTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCa                                                                                                                                               <  1:1569879/150‑1 (MQ=255)
        cTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCa                                                                                                                                               >  2:3370682/1‑150 (MQ=255)
         tCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCAt                                                                                                                                              <  1:3275881/150‑1 (MQ=255)
         tCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCGTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCAt                                                                                                                                              >  2:2801552/1‑150 (MQ=255)
           gCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATTGGTCAGGGAATTAAAAACTAACCCTTACCTTTATTGCGGATGCGATTTATATCGCTTAAGAGTGCAGTACTCATGCCGGATGCGGCTTgt                                                                                                                                            >  2:2542136/1‑149 (MQ=255)
           gCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCCGTGAATTAAAAACTAACCCTTACCTTTAAAGCGTATGCGATTTATATCTCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGa                                                                                                                                            <  1:2289559/150‑1 (MQ=255)
           gCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGa                                                                                                                                            <  1:2731315/150‑1 (MQ=255)
              aGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTa                                                                                                                                         >  2:1981414/1‑150 (MQ=255)
               gCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTAc                                                                                                                                        <  1:2135001/150‑1 (MQ=255)
               gCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGCACTCATGCCGCATCCGGCATGAGTAc                                                                                                                                        <  2:136491/150‑1 (MQ=255)
                   tGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCata                                                                                                                                    >  1:64926/1‑150 (MQ=255)
                     aaaCCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCCTAtc                                                                                                                                  >  2:2378999/1‑150 (MQ=255)
                       aCCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGAGGCGGCATGAGTACCATAtcct                                                                                                                                >  1:3990124/1‑150 (MQ=255)
                        ccAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtcctt                                                                                                                               <  1:913617/150‑1 (MQ=255)
                         cAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTCTATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttc                                                                                                                              >  2:2123651/1‑150 (MQ=255)
                         cAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttc                                                                                                                              <  2:2327230/150‑1 (MQ=255)
                          aaTCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttcc                                                                                                                             <  1:591482/150‑1 (MQ=255)
                          aaTCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttcc                                                                                                                             <  2:3795808/150‑1 (MQ=255)
                          aaTCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttcc                                                                                                                             <  1:3121854/150‑1 (MQ=255)
                          aaTCTCTTCAACAGCGCGCGAAATAGTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttcc                                                                                                                             <  1:3633235/150‑1 (MQ=255)
                           aTCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttcct                                                                                                                            >  2:587739/1‑150 (MQ=255)
                              tctTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAATCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGaa                                                                                                                         >  2:1878996/1‑150 (MQ=255)
                                ttCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGaaaa                                                                                                                       >  2:2672189/1‑150 (MQ=255)
                                  cAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATc                                                                                                                     <  2:342981/150‑1 (MQ=255)
                                    aCAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATcgc                                                                                                                   <  1:3394231/150‑1 (MQ=255)
                                    aCAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATcgc                                                                                                                   <  2:1129926/150‑1 (MQ=255)
                                     cAGCGCGCGCAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATcgcg                                                                                                                  <  2:2992414/150‑1 (MQ=255)
                                     cAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATcgcg                                                                                                                  <  2:4044771/150‑1 (MQ=255)
                                     cAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAAACCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATcgcg                                                                                                                  <  1:254935/150‑1 (MQ=255)
                                      aGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCgcgc                                                                                                                 >  2:3939613/1‑150 (MQ=255)
                                         gcgcgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCaaa                                                                                                              <  2:492650/150‑1 (MQ=255)
                                            cgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTc                                                                                                           >  2:33400/1‑150 (MQ=255)
                                                 tatTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCTCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATACtatat                                                                                                      >  1:3774666/1‑150 (MQ=255)
                                                 tatTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCtatat                                                                                                      >  1:3774237/1‑150 (MQ=255)
                                                     gCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCACGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTg                                                                                                  <  2:473243/150‑1 (MQ=255)
                                                      ccTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTAGATATTGc                                                                                                 >  2:2085585/1‑150 (MQ=255)
                                                       cTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCa                                                                                                <  1:6902/150‑1 (MQ=255)
                                                         gcgcCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCaga                                                                                              <  1:2233047/150‑1 (MQ=255)
                                                            ccATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCTGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATTTTGCAGAGAt                                                                                           <  1:191603/150‑1 (MQ=255)
                                                            ccATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGAGGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAGTGCTATAGAGTGCAgagag                                                                                           >  1:4094903/1‑149 (MQ=255)
                                                              aTTAAAATAGGTCCGGGAATTAAAAACTAACCCTTTCCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGTTGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCCAATTCTATATATTGCAGAGATcc                                                                                         >  2:2198257/1‑149 (MQ=255)
                                                              aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCACATTCTATATATTGCCGAGATCa                                                                                         >  2:7532/1‑150 (MQ=255)
                                                                           aGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATa                                                                            <  1:1234796/150‑1 (MQ=255)
                                                                            gggAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaa                                                                           <  1:500423/150‑1 (MQ=255)
                                                                            gggAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaa                                                                           <  1:500307/150‑1 (MQ=255)
                                                                            gggAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaa                                                                           <  1:3952348/150‑1 (MQ=255)
                                                                             ggAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaag                                                                          <  1:1419439/150‑1 (MQ=255)
                                                                             ggAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaag                                                                          <  1:1525176/150‑1 (MQ=255)
                                                                              gAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagc                                                                         <  2:1231117/150‑1 (MQ=255)
                                                                              gAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagc                                                                         <  1:2183619/150‑1 (MQ=255)
                                                                                 ttAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaa                                                                      >  1:1663768/1‑150 (MQ=255)
                                                                                  tAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaag                                                                     >  1:3759605/1‑150 (MQ=255)
                                                                                     aaaCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAgcgc                                                                  >  1:3474400/1‑150 (MQ=255)
                                                                                      aaCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCa                                                                 >  1:1274995/1‑150 (MQ=255)
                                                                                         tAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCgcagca                                                              >  2:136401/1‑150 (MQ=255)
                                                                                         tAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCa                                                              >  1:2641175/1‑150 (MQ=255)
                                                                                         tAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCa                                                              >  1:556794/1‑150 (MQ=255)
                                                                                         tAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCa                                                              <  2:2603681/150‑1 (MQ=255)
                                                                                             ccTTACCTTTAAAGCGGATGCGATTTATAAAGCATAAGAGTGCAGTACTCATGCCGGATGCGTAATGAGTACAATATCCATACTGAAAATCGCGCAAAATCAATATATTGCAGAGAACATGTAGGCCTGATAAGCGAATCGCATCAGGCa                                                          >  2:1556113/1‑150 (MQ=255)
                                                                                              cTTACCTTTAAATCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGACGGATGCGGCATGAGTACCATATCCTTACTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATTTAGGCCTGATAAGCGAAGCGCATCAGGCaa                                                         <  2:2303224/150‑1 (MQ=255)
                                                                                              cTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCaa                                                         <  2:1274995/150‑1 (MQ=255)
                                                                                              cTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCaa                                                         <  2:1455109/150‑1 (MQ=255)
                                                                                               ttACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAAt                                                        >  1:2346987/1‑150 (MQ=255)
                                                                                                 aCCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGt                                                      >  1:2038581/1‑150 (MQ=255)
                                                                                                   cTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTa                                                    >  1:3453278/1‑150 (MQ=255)
                                                                                                    tttAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTAc                                                   >  1:3846333/1‑150 (MQ=255)
                                                                                                    tttAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTAc                                                   <  1:2466675/150‑1 (MQ=255)
                                                                                                         aGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCGACCTGAAAATCGCGCAAATTCAATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACcaaaa                                              >  1:3058674/1‑150 (MQ=255)
                                                                                                          gCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACaaaaaa                                             >  2:3180789/1‑150 (MQ=255)
                                                                                                           cGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACaaaaaaa                                            <  2:324234/150‑1 (MQ=255)
                                                                                                             gATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGc                                          <  2:626707/150‑1 (MQ=255)
                                                                                                                 cGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACg                                      >  2:3218838/1‑150 (MQ=255)
                                                                                                                         atCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAAc                              >  2:3584005/1‑150 (MQ=255)
                                                                                                                           cGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCg                            >  1:1212886/1‑150 (MQ=255)
                                                                                                                             cATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg                          <  2:3911420/150‑1 (MQ=255)
                                                                                                                             cATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATGGCAGAGATCATGTAGGCCTGATACGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg                          <  2:1771786/150‑1 (MQ=255)
                                                                                                                              aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTgg                         <  2:4094053/150‑1 (MQ=255)
                                                                                                                               tAAGCGTGCAGTACTCCTGCCGGATGCGGCATGAGTACCCTATCCTTCCTGAAATTCGCGCAAATTCTATATATTGCAGAGTTCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTCCAATAAAAGCCACGGTATAAACCGTGGc                        <  2:3883756/150‑1 (MQ=37)
                                                                                                                                aaGAGTGCAGAACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGACGCGCATCAGGCACTGTTACACAAAAAGCCACGGTATAAACCGTGGCa                       >  2:3116210/1‑150 (MQ=255)
                                                                                                                                  gagTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAACTCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCaaa                     <  2:1172276/150‑1 (MQ=255)
                                                                                                                                    gTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAAt                   <  2:2268973/150‑1 (MQ=255)
                                                                                                                                      gCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCACAGATCAGGAAGGCCTGATAAGCGAAGCGCATAAGGCAATGTTACAAACAAAGCCACGGTATAAACCGTGGCAAAATcc                 >  2:3147117/1‑150 (MQ=39)
                                                                                                                                          tACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCACATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACa             >  2:671781/1‑150 (MQ=255)
                                                                                                                                             tCATGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAg          >  1:1469227/1‑150 (MQ=255)
                                                                                                                                             tCATGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGGGGCAAAATCCAACATAg          >  1:1609002/1‑150 (MQ=255)
                                                                                                                                              cATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATTTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGc         <  1:2885277/150‑1 (MQ=255)
                                                                                                                                               aTGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCt        >  1:4123445/1‑150 (MQ=255)
                                                                                                                                               aTGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCt        >  1:2793413/1‑150 (MQ=255)
                                                                                                                                                    ggATGCGTCATGAGTACAATATCCTTCCTGTAAATCTCGAAAATTCTATATATTGAAGTGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCATTGTTACAAAAAAAGCCAAGGTATAAACCGTGGCAAAATCCAACATAGCTaaaaa   <  1:2343449/150‑1 (MQ=38)
                                                                                                                                                     gATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAATATCCAACATAGCTAAAaat  >  1:1009809/1‑150 (MQ=255)
                                                                                                                                                     gATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAaat  >  1:1553181/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAAT  >  NC_000913/542942‑543240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: