Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,158 A→C 9.2% intergenic (+141/‑57) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1580AC 9.2% 456.7 / 22.4 152intergenic (+141/‑57)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (135/2);  new base C (4/11);  total (139/13)
Fisher's exact test for biased strand distribution p-value = 5.83e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

GCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATC  >  NC_000913/3781009‑3781307
                                                                                                                                                       |                                                                                                                                                     
gCGGCATAGTCGTTTGCGCCCCCTCACCCAACCCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCGCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaa                                                                                                                                                       <  2:3830004/150‑1 (MQ=255)
  ggCTTAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                                                                                                     <  1:905582/150‑1 (MQ=255)
  ggCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGTGGGCCGGGGTGAGGGGaaaa                                                                                                                                                     <  1:1622636/150‑1 (MQ=255)
  ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                                                                                                     <  2:3129770/150‑1 (MQ=255)
  ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                                                                                                     <  2:2344421/150‑1 (MQ=255)
  ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCGCCGGGGAGTGGGCCGGGGTGAGGGGaaaa                                                                                                                                                     <  1:905547/150‑1 (MQ=255)
            tttGCGCCCCCTCACCCAAAACCTCACCCACAGGGAGAGGGGACCCTTCGGCGCAGTATTTTCACACGAAAACTGAAAAGACACCGCACACTTTTTTTAT‑‑CACACGCCCCACCCGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGcccag                                                                                                                                           >  2:3161583/1‑146 (MQ=255)
            tttGCGCCCCCTCAACCAAACCCACAACCACAGGGAGAGGGGACCGGTCCGCGCAGTATTTAAACCCTCACACTGAAACCACACCCCACACATTTTTTCT‑‑CCCACGCCCCACCGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCCCAcag                                                                                                                                           >  2:3161437/1‑148 (MQ=255)
                 gcCCCCTCACCCTAACCCACTCCCACAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCACACTCTGAAACGACACCGCACTCTTTTTATAT‑‑ACCTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                                                                                                                      >  1:2249341/1‑150 (MQ=255)
                           ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                                                                            >  1:1370519/1‑150 (MQ=255)
                            cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGCGAGGGCCGGGGGGAGGGGACAAGGCCGCACTGCTCACCAATTTTCTGc                                                                                                                           >  2:270167/1‑150 (MQ=255)
                            cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCCGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                                                                           >  1:2429423/1‑150 (MQ=255)
                                 ccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                                                      >  2:2111775/1‑150 (MQ=255)
                                 ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                                                      >  1:1857978/1‑150 (MQ=255)
                                 ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                                                      >  1:3345401/1‑150 (MQ=255)
                                 ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                                                      >  1:3538739/1‑150 (MQ=255)
                                  ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTCTCCt                                                                                                                     >  2:3458714/1‑150 (MQ=255)
                                  ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                                                     >  1:3681837/1‑150 (MQ=255)
                                   tctcCCTCAGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGCGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATGTTCTGCTATCCTg                                                                                                                    >  2:2134771/1‑150 (MQ=255)
                                   tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                                                    >  1:1910502/1‑150 (MQ=255)
                                       ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                                                                                >  2:1249098/1‑150 (MQ=255)
                                       ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                                                                                >  2:463386/1‑150 (MQ=255)
                                         tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                                                                              >  1:2506289/1‑150 (MQ=255)
                                             ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                                                                          >  2:1262896/1‑150 (MQ=255)
                                             ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                                                                          >  2:2928204/1‑150 (MQ=255)
                                                 agGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                                                      >  1:1695456/1‑150 (MQ=255)
                                                   gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                                                      >  2:1818012/1‑150 (MQ=255)
                                                   gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                                                    >  1:1048510/1‑150 (MQ=255)
                                                   gggACCGTTCGGCGCTGTATGTACTCCCTCACACTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATACTGCACCCGCACTAAgggg                                                                                                    >  1:1678808/1‑150 (MQ=255)
                                                    ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                                                                                   >  1:667005/1‑150 (MQ=255)
                                                    ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                                                                                   >  1:3866422/1‑150 (MQ=255)
                                                     gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                                                                                  >  2:849205/1‑150 (MQ=255)
                                                        cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                                               >  2:1863116/1‑150 (MQ=255)
                                                        cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                                               >  2:2626703/1‑150 (MQ=255)
                                                          ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                                                                             >  2:1727826/1‑150 (MQ=255)
                                                          ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCACGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                                                                             >  1:3179784/1‑150 (MQ=255)
                                                             ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                                                                                          >  2:3329546/1‑150 (MQ=255)
                                                                 cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCCGTATGCTAAAc                                                                                      >  2:1475544/1‑150 (MQ=255)
                                                                 cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAAc                                                                                      >  2:1084836/1‑150 (MQ=255)
                                                                   gtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt                                                                                    >  2:219280/1‑150 (MQ=255)
                                                                        taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGTGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                                                                               >  1:2130943/1‑150 (MQ=255)
                                                                        taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTag                                                                               >  1:2033271/1‑149 (MQ=255)
                                                                         aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                                                                              >  1:983947/1‑150 (MQ=255)
                                                                             ccTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                                                          >  2:3707773/1‑150 (MQ=255)
                                                                              cTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                                                                         >  1:1714550/1‑150 (MQ=255)
                                                                              cTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                                                                         >  2:612058/1‑150 (MQ=255)
                                                                               tCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                                                        >  1:1068758/1‑150 (MQ=255)
                                                                                   tctGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                                                                    >  1:3708402/1‑150 (MQ=255)
                                                                                   tctGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                                                                    >  1:2631006/1‑150 (MQ=255)
                                                                                   tctGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTACGGGGAGATGGCCGGGGTGATGGGAAAATGCCGCACAGCTAAACAATATTCTGCTATACTGCCCCCGCAATAAGGGGTCAGTATGCTAAACATCGTACTTTACGAACCa                                                                    >  2:412024/1‑150 (MQ=255)
                                                                                    ctGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                                                   >  1:2447923/1‑150 (MQ=255)
                                                                                    ctGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGACGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                                                   >  1:631167/1‑150 (MQ=255)
                                                                                     tGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAAGCTAAACATCGTACTTTACGAACCAGa                                                                  >  1:534124/1‑150 (MQ=255)
                                                                                           gACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc                                                            >  1:3504009/1‑150 (MQ=255)
                                                                                           gACACCGCACTCTTTTTTTCT‑‑ACCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGCGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc                                                            >  2:1874020/1‑150 (MQ=255)
                                                                                                cGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                                                       >  1:558361/1‑150 (MQ=255)
                                                                                                cGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                                                       >  1:1316379/1‑150 (MQ=255)
                                                                                                cGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                                                       >  2:3538005/1‑150 (MQ=255)
                                                                                                cGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                                                       >  2:1572255/1‑150 (MQ=255)
                                                                                                cGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                                                       >  2:2152409/1‑150 (MQ=255)
                                                                                                     tctTTTTTCCC‑‑CCCCCCCCCCGCGGGGGGGGGGGGGGGGGGGCGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                                                  <  2:2740961/150‑1 (MQ=255)
                                                                                                       tttttttCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                >  2:61483/1‑150 (MQ=255)
                                                                                                       tttttttCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                >  2:898736/1‑150 (MQ=255)
                                                                                                       tttttttCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                >  2:3926735/1‑150 (MQ=255)
                                                                                                       tttttttCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                >  1:2363395/1‑150 (MQ=255)
                                                                                                       tttttttCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                >  1:2470919/1‑150 (MQ=255)
                                                                                                          ttttCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCac                                             >  1:181115/1‑149 (MQ=255)
                                                                                                            ttcgcccccTCGCCCGGCGGGGGTGTGGGCGGGGGGGCGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCaa                                             <  1:3767153/144‑1 (MQ=255)
                                                                                                               t‑‑cCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca                                        >  1:849201/1‑150 (MQ=255)
                                                                                                                    cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc                                     >  2:1269612/1‑150 (MQ=255)
                                                                                                                    cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc                                     >  1:2112885/1‑150 (MQ=255)
                                                                                                                    cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc                                     >  2:3630461/1‑150 (MQ=255)
                                                                                                                    cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc                                     >  2:2593719/1‑150 (MQ=255)
                                                                                                                    cTCCCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGACAAGGCCGCACTGCTCACCAATTGTCTGCTATCCTGCCCCCGCACTCAGGGGGCAGTATGCTCAACATCGTACTTTACGAACCAGAAATTCCGCCACATACTCGCAACATCATcc                                     >  2:3828943/1‑150 (MQ=255)
                                                                                                                     tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg                                    >  1:2869725/1‑150 (MQ=255)
                                                                                                                     tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg                                    >  2:3764864/1‑150 (MQ=255)
                                                                                                                      ccccccTGGGGGGTGGGGGGGGGGGTGGGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGt                                   <  1:4023754/148‑1 (MQ=255)
                                                                                                                      cGCCCGTCGGGGGCGTGGGGGGGGGTGTGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGt                                   <  1:3105002/150‑1 (MQ=255)
                                                                                                                      cGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGt                                   >  2:4099642/1‑150 (MQ=255)
                                                                                                                          ccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt                               >  1:1315426/1‑150 (MQ=255)
                                                                                                                          ccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt                               >  1:3355850/1‑150 (MQ=255)
                                                                                                                             ggggggTGGGGGCGGGGTGGTGGGGAAAGGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAATGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgcg                            <  1:3024439/148‑1 (MQ=255)
                                                                                                                                gggAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTa                         >  2:52906/1‑150 (MQ=255)
                                                                                                                                  gagaGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAAt                       >  1:2509335/1‑150 (MQ=255)
                                                                                                                                  gagaGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAAt                       >  1:3368262/1‑150 (MQ=255)
                                                                                                                                     agGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATAcc                    >  1:1836964/1‑150 (MQ=255)
                                                                                                                                      gggggggggTGGGGGCCCAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCg                   <  2:2249341/145‑1 (MQ=255)
                                                                                                                                      gggggggggTGGGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCg                   <  2:3043673/145‑1 (MQ=255)
                                                                                                                                      gggggggggTGCGCGAACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCg                   <  2:4064998/145‑1 (MQ=255)
                                                                                                                                       ggCGGGGGTGCGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCgg                  <  2:2607090/150‑1 (MQ=255)
                                                                                                                                       ggCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCgg                  >  2:2141351/1‑150 (MQ=255)
                                                                                                                                        gCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGc                 >  2:676129/1‑150 (MQ=255)
                                                                                                                                          cGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACAGGCtt               >  2:3579282/1‑150 (MQ=255)
                                                                                                                                               tGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTc          >  2:2122568/1‑150 (MQ=255)
                                                                                                                                                   gggAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCa      >  2:1331208/1‑150 (MQ=255)
                                                                                                                                                   gggAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCa      >  2:3235730/1‑150 (MQ=255)
                                                                                                                                                   gggAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCa      >  2:2091687/1‑150 (MQ=255)
                                                                                                                                                     gAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCata    >  1:2177773/1‑150 (MQ=255)
                                                                                                                                                      aaaaGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCatat   >  1:1694333/1‑150 (MQ=255)
                                                                                                                                                       aaaGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATatc  >  2:2022282/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
GCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCT‑‑CCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATC  >  NC_000913/3781009‑3781307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: