Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,086,965 G→T 6.8% intergenic (+116/‑37) fdhD → / → yiiG formate dehydrogenase formation protein/DUF3829 family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,086,9650GT 6.8% 651.5 / 4.7 213intergenic (+116/‑37)fdhD/yiiGformate dehydrogenase formation protein/DUF3829 family lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (81/110);  new base T (20/2);  total (101/112)
Fisher's exact test for biased strand distribution p-value = 1.54e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

GTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCA  >  NC_000913/4086817‑4087114
                                                                                                                                                    |                                                                                                                                                      
  ttATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTc                                                                                                                                                     <  1:1165778/150‑1 (MQ=255)
  ttATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTc                                                                                                                                                     <  1:1185257/150‑1 (MQ=255)
    ataCCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATACATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCAc                                                                                                                                                   <  1:687595/150‑1 (MQ=255)
     taCCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCAct                                                                                                                                                  <  1:2329096/150‑1 (MQ=255)
        ccATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCActctc                                                                                                                                               >  2:2095817/1‑150 (MQ=255)
        ccATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTAActctc                                                                                                                                               >  2:811480/1‑150 (MQ=255)
          aTCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCtt                                                                                                                                             >  1:1727992/1‑150 (MQ=255)
             cGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGGAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTGTTATc                                                                                                                                          <  1:2443131/150‑1 (MQ=255)
             cGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATc                                                                                                                                          <  2:3541313/150‑1 (MQ=255)
             cGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGAGTGTAGTGGCGTTTTCTTTTTTAATTTATTATTTTAAATATATTATc                                                                                                                                          >  2:1948682/1‑150 (MQ=255)
              gCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCt                                                                                                                                         <  1:982529/150‑1 (MQ=255)
                  cGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCtatat                                                                                                                                     <  1:2942850/150‑1 (MQ=255)
                   gTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCtatata                                                                                                                                    <  2:1894176/150‑1 (MQ=255)
                    tttGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTatatat                                                                                                                                   >  2:1373908/1‑150 (MQ=255)
                    tttGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCACATATCGatatat                                                                                                                                   >  1:320814/1‑150 (MQ=255)
                     ttGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATAtt                                                                                                                                  >  2:2703968/1‑150 (MQ=255)
                        aGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTtat                                                                                                                               <  1:555657/150‑1 (MQ=255)
                        aGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTtat                                                                                                                               <  2:1218421/150‑1 (MQ=255)
                        aGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTtat                                                                                                                               <  1:290656/150‑1 (MQ=255)
                         gCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTGATTTATTATTTTGAATATATTATAtttatatttata                                                                                                                              >  2:3474382/1‑150 (MQ=255)
                         gCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTtata                                                                                                                              <  2:860150/150‑1 (MQ=255)
                         gCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTtata                                                                                                                              <  1:2850324/150‑1 (MQ=255)
                          cAATTAATTAAAATAAGTCCTGCGAAATTTATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGTCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATaa                                                                                                                             <  2:2464854/150‑1 (MQ=255)
                          cAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATaa                                                                                                                             <  2:4099242/150‑1 (MQ=255)
                           aattaattAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTGACTCTCTGATCTATATATTTATAAg                                                                                                                            >  1:3341958/1‑150 (MQ=255)
                            attaattaAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAgg                                                                                                                           <  2:3710022/150‑1 (MQ=255)
                            attaattaAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAgg                                                                                                                           <  2:1982681/150‑1 (MQ=255)
                            attaattaAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTATTTTTTTGACTCTCTTATATATATATTTATaatg                                                                                                                           >  1:3126045/1‑148 (MQ=255)
                                 ttaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTCTTTTTTGATTTATTATGTTGACTCTCTTATGTATATATTTATAAGGCAAtt                                                                                                                     >  1:181950/1‑150 (MQ=255)
                                    aaaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTaaa                                                                                                                  <  2:415172/150‑1 (MQ=255)
                                    aaaTAAGTCCTGCGAAATAGAATAAAGTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCTTTTATTATGTTCACTCTCTTATCTATATTTTTATAAGGCAATTaaa                                                                                                                  <  1:3366104/150‑1 (MQ=255)
                                     aaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTTATTTTTTATGTTGACGGTGTTATCTTTATATTTTTTATGGAATTTAAt                                                                                                                 >  1:3881553/1‑150 (MQ=255)
                                     aaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTAATTTATTATTTTGACACTCATATGTATATATTTATAAGGCaaataaat                                                                                                                 >  2:20359/1‑150 (MQ=255)
                                     aaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATTTTCACTCTCTTATCTATATATTTATAAGGCAATTAAAt                                                                                                                 >  2:175217/1‑150 (MQ=255)
                                     aaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAAt                                                                                                                 >  2:2418021/1‑150 (MQ=255)
                                     aaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAAt                                                                                                                 <  2:873111/150‑1 (MQ=255)
                                     aaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAAt                                                                                                                 >  1:1386153/1‑150 (MQ=255)
                                     aaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTCTTTTTTAATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCCATTAAAt                                                                                                                 >  1:1386020/1‑150 (MQ=255)
                                        aaGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaa                                                                                                              <  2:1096071/150‑1 (MQ=255)
                                         aGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaaa                                                                                                             <  1:1754363/150‑1 (MQ=255)
                                          gTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaaaa                                                                                                            <  1:4085093/150‑1 (MQ=255)
                                          gTCCTGCGAAATATATTAAAGTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaaaa                                                                                                            <  1:743876/150‑1 (MQ=255)
                                           tCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCAATCTATTATCTATATATTTATAAGGCAATTAAATGAAAAg                                                                                                           >  2:2996267/1‑150 (MQ=255)
                                           tCCTGCGAAATATATAAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTTTAAGGCAATTAAATGAAAAg                                                                                                           >  1:1028102/1‑150 (MQ=255)
                                              tGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGGTCCCGCACGTAACGATATATTTATAAGGCAAATAAAATAAAAAGGa                                                                                                         >  1:3473080/1‑150 (MQ=255)
                                              tGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTATTATGTTCACTCTCTTATATATATATTTATAAGGCAATTAAATGAAAAGGaa                                                                                                        >  1:2384501/1‑150 (MQ=255)
                                              tGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTCATTTTTAATTTATTATGGTCACTATCATATCTATATATTTATAAGGCAATTAAATGAAAAGGaa                                                                                                        >  1:1966120/1‑150 (MQ=255)
                                               gCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAAt                                                                                                       <  1:2327060/150‑1 (MQ=255)
                                                  aaaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATTTTAAATCTCTTATATATATATTTATAATGCAATTAAATGAAAAGGAAtttt                                                                                                    >  1:2239589/1‑149 (MQ=255)
                                                  aaaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTTTTATTTTGAATATCTTTTATATATATTTTTAATGCCATTAAAATAAAAggcaagta                                                                                                    >  1:2786417/1‑144 (MQ=255)
                                                   aaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATttat                                                                                                   <  1:3567957/150‑1 (MQ=255)
                                                        atTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTATTATTTTCACTCTCGTATGTATATATTTATAAGGCAAATAAATGTAAAGGAATTtattatat                                                                                              >  1:909198/1‑148 (MQ=255)
                                                        atTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTTTTATGTTCACTATCTTATCTATATATTTATAATGGAATTAAATGAAAAGTAATTTATTATCt                                                                                              >  1:909116/1‑150 (MQ=255)
                                                            aaTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTTTTTTGTTGAATCTCTTATCTATATATTTATAAGGCAAATAAATTTAAAGGAATTTATTATATTACg                                                                                          >  1:3744170/1‑150 (MQ=255)
                                                             aTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATTTTAACTCTCTTATCTATATATTTATAAGGCAATTAAATTAAAAGGAATTTATTATAttaagc                                                                                         >  2:1631122/1‑146 (MQ=255)
                                                                 cAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGTGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtatt                                                                                     <  1:459210/150‑1 (MQ=255)
                                                                 cAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtatt                                                                                     <  2:3918360/150‑1 (MQ=255)
                                                                    gACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATtata                                                                                  <  1:1218169/150‑1 (MQ=255)
                                                                       ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTc                                                                               <  1:2991810/150‑1 (MQ=255)
                                                                           tCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCca                                                                           <  1:768866/150‑1 (MQ=255)
                                                                           tCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCca                                                                           <  1:769224/150‑1 (MQ=255)
                                                                            cATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCcat                                                                          <  1:2012035/150‑1 (MQ=255)
                                                                                  gTGAATTTTATTATTTTATTTATAAACAAAATGTGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAATAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTc                                                                    >  2:3019690/1‑150 (MQ=255)
                                                                                  gTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGTAAATGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTc                                                                    >  2:3747013/1‑150 (MQ=255)
                                                                                          tattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTc                                                            <  1:579009/150‑1 (MQ=255)
                                                                                          tattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTc                                                            <  1:4005778/150‑1 (MQ=255)
                                                                                          tattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTACGCTATTATAGTCGCCATCATGTCCCTCGGTc                                                            >  2:3370765/1‑150 (MQ=255)
                                                                                          tattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCAATATCTTATCTATATATTTATAAGGCAATTAAATGAAAATGAATTTATTATCTTCCGCTATTATAGGCGCCATCATGTACCTCGGTc                                                            >  2:3700383/1‑150 (MQ=255)
                                                                                               ttttatttATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACg                                                       <  2:3299920/150‑1 (MQ=255)
                                                                                                 ttatttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTGTCTTATGTATATATTTTTAAGGCAATTAAATGGAAAGGGATTTATTATCTTCCGCTATTATAGTCGGCATCATGTCCCTGGGTGTGGCggg                                                     >  2:3984407/1‑150 (MQ=255)
                                                                                                 ttatttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACggg                                                     >  2:1700070/1‑150 (MQ=255)
                                                                                                     ttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTtgt                                                 <  1:3251576/150‑1 (MQ=255)
                                                                                                     ttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTtgt                                                 <  2:3783592/150‑1 (MQ=255)
                                                                                                     ttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCAAGTCCCTCGGTCTGACGGGTtgt                                                 >  2:262151/1‑150 (MQ=255)
                                                                                                      tataAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTATTATTTTCACAATATTATCTATATATTTATAATGCAATTAAAAGAAAAGGAAATTATTATATTACGCTATTATATTCGCCAACCTGTCCCTCGGTCTGACGGGGtgtg                                                >  2:728095/1‑150 (MQ=255)
                                                                                                       ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAATGCAATTAAATGAAAATGAATTTATTATATTCCGCTATTATATTCGCCAACATGTCCCACGGTCTGACGGGTTGtga                                               >  2:2219972/1‑150 (MQ=255)
                                                                                                       ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtga                                               <  2:1276854/150‑1 (MQ=255)
                                                                                                       ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtga                                               <  2:4031875/150‑1 (MQ=255)
                                                                                                       ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtga                                               <  2:1438750/150‑1 (MQ=255)
                                                                                                       ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtga                                               <  2:181950/150‑1 (MQ=255)
                                                                                                       ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATCATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtga                                               <  2:1561147/150‑1 (MQ=255)
                                                                                                        taAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtgat                                              <  1:2511590/150‑1 (MQ=255)
                                                                                                          aaCAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATTAAAAGGAATTTATTATATTACGCTATTATATTAGCCATCATGTCCCTCGGTCTGTCGGGTTGtgatga                                            >  2:2948486/1‑150 (MQ=255)
                                                                                                           aCAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTACGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTgatgat                                           >  2:569734/1‑150 (MQ=255)
                                                                                                           aCAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTgatgat                                           >  2:3502191/1‑150 (MQ=255)
                                                                                                            cAAAATGCGTGTGGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATATTACGCTATTATAGTCGCCAACATGTCCCTCGGTCTGACGGGTTGTGATGATa                                          >  2:3131590/1‑150 (MQ=255)
                                                                                                            cAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATa                                          <  2:3850035/150‑1 (MQ=255)
                                                                                                            cAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAATTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATa                                          >  2:3042103/1‑150 (MQ=255)
                                                                                                             aaaaTGCGTGTAGTGGCGTTTTCTTTTTTCAATTATTATGTTCACTCTCGTATCTATATAGTTATAAGGCAAATAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATTTCCCACGGTCTGACGGGTGGTGATGATaa                                         >  2:2820399/1‑150 (MQ=255)
                                                                                                               aaTGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATaaaa                                       <  2:3499360/150‑1 (MQ=255)
                                                                                                                 tGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATaaaaaa                                     <  2:440219/150‑1 (MQ=255)
                                                                                                                   cGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGc                                   <  1:2552493/150‑1 (MQ=255)
                                                                                                                    gtgtAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGcc                                  <  2:648913/150‑1 (MQ=255)
                                                                                                                        tgTGGCTTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGaaa                              <  1:1445844/149‑1 (MQ=255)
                                                                                                                                 ttCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGc                     >  2:3603428/1‑150 (MQ=255)
                                                                                                                                  tCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGTAATTTATTATCTTCCGATATTATAGTCGCCATCTTGTCCCTCGGTCTGACGGGTTGTGATTATAAAAAAGCCGAAACAGAAACGCt                    <  1:2268558/150‑1 (MQ=255)
                                                                                                                                    ttttttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTcc                  >  2:634570/1‑150 (MQ=255)
                                                                                                                                            tttATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGcc          >  2:3348779/1‑150 (MQ=255)
                                                                                                                                              tattatGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCaa        >  1:1902220/1‑150 (MQ=255)
                                                                                                                                                ttatGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATa      >  2:3140831/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCA  >  NC_000913/4086817‑4087114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: