Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,614,378 A→G 7.2% intergenic (+118/+302) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,614,3780AG 7.2% 874.0 / 35.1 280intergenic (+118/+302)yjjV/yjjWputative DNase/putative pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (79/176);  new base G (13/7);  total (94/186)
Fisher's exact test for biased strand distribution p-value = 2.97e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

TAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGC  >  NC_000913/4614230‑4614527
                                                                                                                                                    |                                                                                                                                                     
taaCACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAc                                                                                                                                                      <  2:843217/150‑1 (MQ=255)
taaCACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAc                                                                                                                                                      <  2:3121677/150‑1 (MQ=255)
  acacGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                                                    <  1:3754797/150‑1 (MQ=255)
  acacGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                                                    <  2:1363/150‑1 (MQ=255)
   cacGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGcgcc                                                                                                                                                   <  2:1739789/150‑1 (MQ=11)
    acGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATCAGGCGTTCGcgccg                                                                                                                                                  >  2:2120717/1‑150 (MQ=255)
    acGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgccg                                                                                                                                                  >  2:1576998/1‑150 (MQ=255)
        ataCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                                                              <  1:3112273/150‑1 (MQ=255)
        ataCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                                                              <  2:3867934/150‑1 (MQ=255)
          aCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATTAGGCGTTCACGCCGCATCCg                                                                                                                                            <  1:3643711/150‑1 (MQ=255)
          aCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                                            <  2:4023819/150‑1 (MQ=255)
           cggtgttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                           <  2:3250077/147‑1 (MQ=255)
           cGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                           <  2:2236841/150‑1 (MQ=255)
                tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTGTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGtt                                                                                                                                      >  2:768214/1‑150 (MQ=255)
                  tAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTgg                                                                                                                                    <  2:3953338/150‑1 (MQ=255)
                   aaCGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgc                                                                                                                                   <  1:2041287/150‑1 (MQ=255)
                   aaCGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgc                                                                                                                                   <  2:3457919/150‑1 (MQ=255)
                      gTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTCACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGca                                                                                                                                >  2:3574466/1‑150 (MQ=255)
                              ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTGTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCt                                                                                                                        <  2:3454296/150‑1 (MQ=255)
                                ccGGATTAGGCGTTCTCGCCGCATGCTGCAGTTGGCGCACTATGCCTGATGCGACGCTTATCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGa                                                                                                                      <  2:1948699/150‑1 (MQ=11)
                                ccGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGa                                                                                                                      >  1:160564/1‑150 (MQ=37)
                                ccGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGa                                                                                                                      <  1:1049916/150‑1 (MQ=255)
                                ccGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGa                                                                                                                      <  1:1873479/150‑1 (MQ=255)
                                 cGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGAt                                                                                                                     <  1:3589164/150‑1 (MQ=255)
                                  ggATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                                                                                                                    <  2:3707479/150‑1 (MQ=255)
                                     tAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGa                                                                                                                 <  2:630409/150‑1 (MQ=255)
                                     tAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGa                                                                                                                 <  2:1829282/150‑1 (MQ=255)
                                      aaGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGAc                                                                                                                <  2:2718247/150‑1 (MQ=255)
                                           gTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCCGCAGTTGGCGCACAATGCCTGAGGCGACGCtgg                                                                                                           >  2:2450000/1‑148 (MQ=255)
                                              cACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTcg                                                                                                        <  1:295399/150‑1 (MQ=255)
                                               aCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGcgccg                                                                                                                                                  >  1:1317217/1‑107 (MQ=255)
                                                cgccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTcgcg                                                                                                      <  2:963967/150‑1 (MQ=255)
                                                 gccgcATCCGGCAGTTGGCGCACACTGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGt                                                                                                     <  2:2250249/150‑1 (MQ=255)
                                                 gccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTAACATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGt                                                                                                     <  2:539137/150‑1 (MQ=255)
                                                  ccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTGTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTc                                                                                                    >  1:2437200/1‑150 (MQ=255)
                                                       tCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATc                                                                                               <  1:2087657/150‑1 (MQ=255)
                                                        ccGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCa                                                                                              <  2:1636192/150‑1 (MQ=255)
                                                         cGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCAt                                                                                             <  1:2206815/150‑1 (MQ=255)
                                                         cGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCAt                                                                                             <  2:303281/150‑1 (MQ=255)
                                                          ggCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCagg                                                                                            >  2:1612716/1‑148 (MQ=12)
                                                           gCAGTTGGCGCACACTGCCTGCTGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGGGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGc                                                                                           <  1:3805857/150‑1 (MQ=255)
                                                           gCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGc                                                                                           <  1:2537427/150‑1 (MQ=255)
                                                            cAGTTGGCGCACACTGCCTGCTGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGcc                                                                                          <  2:2360069/150‑1 (MQ=255)
                                                            cAGTTGGCGCACAATGCCGGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGcc                                                                                          <  2:2191446/150‑1 (MQ=255)
                                                                    gcACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTc                                                                                  >  1:4064020/1‑150 (MQ=255)
                                                                     cacaATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCt                                                                                 <  1:2146173/150‑1 (MQ=255)
                                                                     cacaATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCt                                                                                 <  1:49416/150‑1 (MQ=255)
                                                                       caAGGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCtgt                                                                               <  1:2922206/150‑1 (MQ=255)
                                                                        aaTGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACCAGTCtgtg                                                                              >  2:706045/1‑150 (MQ=12)
                                                                        aaTGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAAAGCCTGATGCGACGCTTGTCGCGGCGTATCAGGCCTCCAAGTCtgtg                                                                              >  2:2595294/1‑150 (MQ=11)
                                                                        aaTGCCTGAAGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCtgtg                                                                              <  2:4119874/150‑1 (MQ=255)
                                                                         aTGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGc                                                                             <  1:3474785/150‑1 (MQ=255)
                                                                          tGCCTGATGCGACGCTTAACGCGTCTTAACATGCCTACAGGTTTGTGCCGAACCGTAGTAAGGATAAGGCGTTAACGACGCAACCGGCAGTTGGCGCACAAAGCCTGATGCGACGCTTGACGCGTATTATCAGGCCTACAAGTATGAGca                                                                            >  1:3878451/1‑149 (MQ=11)
                                                                              tGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAAc                                                                        >  1:2636948/1‑150 (MQ=255)
                                                                                   cGACGCTTATCGGGTTTTATCATGGCTTCAGGTGTGTGCCGTTCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAg                                                                   <  2:3367978/150‑1 (MQ=11)
                                                                                    gACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCa                                                                                                                                         >  2:1261432/1‑79 (MQ=21)
                                                                                    gACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCa                                                                                                                                         <  1:1261432/79‑1 (MQ=21)
                                                                                     aCGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCgg                                                                                                                                           >  1:3008783/1‑76 (MQ=21)
                                                                                       gCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCg                                                               >  1:3361752/1‑150 (MQ=255)
                                                                                       gCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCg                                                               >  1:2223125/1‑150 (MQ=255)
                                                                                        cTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCgg                                                              >  2:2959026/1‑150 (MQ=255)
                                                                                         ttAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGa                                                             <  2:3630440/150‑1 (MQ=255)
                                                                                          tAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGAt                                                            >  1:1716787/1‑150 (MQ=255)
                                                                                          tAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTcgc                                                                                                       <  2:1716797/107‑1 (MQ=12)
                                                                                               cgTCTTAACATCCCTACAGGATTGTTCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCTCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGc                                                       <  1:772248/150‑1 (MQ=14)
                                                                                                 tCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGt                                                     >  2:2319996/1‑150 (MQ=255)
                                                                                                 tCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGt                                                     >  2:1314259/1‑150 (MQ=255)
                                                                                                  cTTATCATGCCTACAGGTTTGTGCCGATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGtt                                                    <  1:2805399/150‑1 (MQ=255)
                                                                                                  cTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGtt                                                    <  2:286782/150‑1 (MQ=255)
                                                                                                      tCATGCCTACAGGTTTGTGCCGAACCGTAGTCAGGATAAGGCGTTCACGACGCATCCGGCAGTTGTCGCACAATGCCTGATGCGACGCTTGTCGAGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcg                                                >  1:800456/1‑150 (MQ=14)
                                                                                                      tCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCTTCCGGCAGTTGGCGCACAATGCCTGTTGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGATCCGTAGGCCGGATAAGGCGtggccg                                                >  1:3722903/1‑145 (MQ=255)
                                                                                                      tCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcg                                                <  2:1181476/150‑1 (MQ=255)
                                                                                                       cATGCCTACAGGTTTGTGCCGATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                               <  1:2733362/150‑1 (MQ=255)
                                                                                                       cATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                               <  2:1385166/150‑1 (MQ=255)
                                                                                                       cATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                               <  1:4095453/150‑1 (MQ=255)
                                                                                                          gCCTACAGGTGTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgccgc                                            <  2:901664/150‑1 (MQ=255)
                                                                                                             tACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGa                                                                                                                      >  1:1926922/1‑73 (MQ=34)
                                                                                                             tACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATACGGCGTTCACGCCGCATc                                         >  2:2507599/1‑150 (MQ=255)
                                                                                                             tACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                         <  1:3444631/150‑1 (MQ=255)
                                                                                                               cAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                       >  1:2374435/1‑150 (MQ=255)
                                                                                                                aGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                      >  2:4078028/1‑150 (MQ=255)
                                                                                                                  gTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                    >  1:4001014/1‑150 (MQ=255)
                                                                                                                    ttGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGt                                  >  1:155599/1‑150 (MQ=255)
                                                                                                                      gtgCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACTATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                <  2:511814/150‑1 (MQ=255)
                                                                                                                        gCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTAGgc                              >  2:1032632/1‑150 (MQ=255)
                                                                                                                         ccGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGgcg                             >  1:1334048/1‑150 (MQ=255)
                                                                                                                         ccGAACCGTAGGCCGGATAAGGCGATAACGCAGCATCCCGCTGGTGTACAACAATGCCAGATGCGACGCTAGTCGCGTCTAATCATGCCTACAAGTCTGTGCCGAACCGTTGGCCGGATGAGGCGTTCGCGCCGCATCCGGCAGgcggcg                             >  1:1018455/1‑150 (MQ=14)
                                                                                                                               cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGAGTTCACGCCGCATCCGGCAGTCGGCGCATAAt                       <  1:1111899/150‑1 (MQ=255)
                                                                                                                                   ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACACGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCt                   >  2:3510148/1‑150 (MQ=255)
                                                                                                                                     ccGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGa                 <  1:3668538/150‑1 (MQ=255)
                                                                                                                                     ccGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGa                 <  1:143300/150‑1 (MQ=255)
                                                                                                                                     ccGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGa                 <  2:1660851/150‑1 (MQ=255)
                                                                                                                                     ccGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGa                 <  2:3764344/150‑1 (MQ=255)
                                                                                                                                        gATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGc              <  1:3282157/150‑1 (MQ=255)
                                                                                                                                         aTAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGCCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCg             >  2:1873336/1‑150 (MQ=255)
                                                                                                                                           aaGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGAc           <  2:4064020/150‑1 (MQ=255)
                                                                                                                                                gTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACACGTCTGTGCCGACCCGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTCGCCGCACACTGCCTGAGGCGACGCtgg      >  2:2590203/1‑148 (MQ=11)
                                                                                                                                                gTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTg      >  2:3297048/1‑150 (MQ=255)
                                                                                                                                                  tCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTc    <  1:3087706/150‑1 (MQ=255)
                                                                                                                                                    aCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTcgc  <  1:9666/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGC  >  NC_000913/4614230‑4614527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: