Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 720,061 C→T 5.1% G134S (GGC→AGC)  speF ← ornithine decarboxylase isozyme, inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913720,0610CT 5.1% 683.6 / 13.6 195G134S (GGC→AGC) speFornithine decarboxylase isozyme, inducible
Reads supporting (aligned to +/- strand):  ref base C (55/130);  new base T (5/5);  total (60/135)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

CGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCA  >  NC_000913/719912‑720208
                                                                                                                                                     |                                                                                                                                                   
 gCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTcgccg                                                                                                                                                    <  2:2937386/150‑1 (MQ=255)
 gCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTCCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATAcgccg                                                                                                                                                    >  2:859558/1‑150 (MQ=255)
   ttGTTGGTGTGCTATGCTTGGGGGGCCTTGGTGATTCAGCAGATCGCCCATCGCTAGGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCATATTATTCCACAATCTGATTCCCCGCCGGTTGGCGACGTAAAAATTCgccgcc                                                                                                                                                  <  1:391508/149‑1 (MQ=255)
     tttGCTGTGCAATGCTTGGCGCGCCTTCGTTAATCAGCAGATCGCCCATCGCTACGTCGGGGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCGTGATGGCGACGGAAAAATTCGCCGCCCt                                                                                                                                                <  1:1833624/149‑1 (MQ=255)
       tGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCTAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCtga                                                                                                                                              <  2:2160195/150‑1 (MQ=255)
       tGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCtga                                                                                                                                              <  2:3830578/150‑1 (MQ=255)
       tGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCtga                                                                                                                                              <  2:2335155/150‑1 (MQ=255)
       tGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCtga                                                                                                                                              <  1:997898/150‑1 (MQ=255)
       tGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCtga                                                                                                                                              <  2:549180/150‑1 (MQ=255)
            tgCAATGCATGGCGCGCCTTCGTGAATCAGCAGCTCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGAcc                                                                                                                                         <  2:3020343/150‑1 (MQ=255)
             gCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCa                                                                                                                                        <  1:3281106/150‑1 (MQ=255)
               aaTGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAgg                                                                                                                                      <  1:3239138/150‑1 (MQ=255)
               aaTGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAgg                                                                                                                                      <  1:2203605/150‑1 (MQ=255)
                aTGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAggg                                                                                                                                     <  1:51507/150‑1 (MQ=255)
                 tGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGc                                                                                                                                    >  2:3400035/1‑150 (MQ=255)
                   cATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCaa                                                                                                                                  <  2:2072478/150‑1 (MQ=255)
                    aTGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAAt                                                                                                                                 <  1:276874/150‑1 (MQ=255)
                    aTGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAAt                                                                                                                                 <  2:451392/150‑1 (MQ=255)
                     tGGCGCGCCTTCGTGAATCAGCAGATCGCCCCTCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATc                                                                                                                                <  1:2490850/150‑1 (MQ=255)
                     tGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATc                                                                                                                                <  1:1691970/150‑1 (MQ=255)
                        cgcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCaaa                                                                                                                             <  1:1467654/150‑1 (MQ=255)
                         gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAAc                                                                                                                            <  1:2170715/150‑1 (MQ=255)
                         gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAAc                                                                                                                            <  1:2963125/150‑1 (MQ=255)
                             cTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGGAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACgcgc                                                                                                                        <  1:3348412/150‑1 (MQ=255)
                                cGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGt                                                                                                                     <  2:2563310/150‑1 (MQ=255)
                                 gTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGtt                                                                                                                    >  2:2173690/1‑150 (MQ=255)
                                 gTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGtt                                                                                                                    <  1:2530708/150‑1 (MQ=255)
                                   gAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTAc                                                                                                                  <  1:1377015/150‑1 (MQ=255)
                                     aTCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCt                                                                                                                <  1:960652/150‑1 (MQ=255)
                                      tCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAATACGGCACCGAACAGCTCCTCACCAAAGTAAAACACAAAATGAAACCCCGCCCGAAGGCGACGGAAAAATTCGCCGCCCTGATGAACACGGCAAAAACACGCGCTGTTaacat                                                                                                               >  1:3884255/1‑146 (MQ=255)
                                      tCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCtt                                                                                                               >  2:4110729/1‑150 (MQ=255)
                                        agcagATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTAATCCACAAACTGATTCCCCGCAGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCttga                                                                                                             >  1:4035721/1‑150 (MQ=255)
                                          cagATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCttgatt                                                                                                           <  1:1560395/150‑1 (MQ=255)
                                          cagATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCttgatt                                                                                                           <  1:1961491/150‑1 (MQ=255)
                                          cagATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGACTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCttgatt                                                                                                           <  1:175166/150‑1 (MQ=255)
                                           agATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACTGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCttgattg                                                                                                          <  2:3037376/150‑1 (MQ=255)
                                           agATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACTGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCttgattg                                                                                                          <  2:3823942/150‑1 (MQ=255)
                                            gATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCttgattga                                                                                                         <  2:1005669/150‑1 (MQ=255)
                                             aTCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGAc                                                                                                        <  2:1060198/150‑1 (MQ=255)
                                                  ccATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAAt                                                                                                   >  2:194727/1‑150 (MQ=255)
                                                  ccATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAAt                                                                                                   <  2:2428750/150‑1 (MQ=255)
                                                   cATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATc                                                                                                  <  1:3506465/150‑1 (MQ=255)
                                                   cATCGCTACATCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATc                                                                                                  <  2:962982/150‑1 (MQ=255)
                                                    aTCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCg                                                                                                 >  2:3237082/1‑150 (MQ=255)
                                                          aCGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGt                                                                                           <  2:612962/150‑1 (MQ=255)
                                                            gTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTgc                                                                                         >  1:1790815/1‑150 (MQ=255)
                                                             tCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCCTGATTGACATAATCGACCAGTgcg                                                                                        >  1:2254101/1‑150 (MQ=255)
                                                              cGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTgcgc                                                                                       <  2:3780751/150‑1 (MQ=255)
                                                                gCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCTGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCgg                                                                                     >  2:4071979/1‑150 (MQ=255)
                                                                 cGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGga                                                                                    >  1:107112/1‑150 (MQ=255)
                                                                  gTTGCACATGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGTTGGCGACGTAAAATTTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGgaa                                                                                   <  1:2076259/150‑1 (MQ=255)
                                                                      cacaAGTCGGCACGGAACAGCGCCTCACCAACGTATTCCACAACCTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAACCGCGCGGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGaaa                                                                               <  1:2586445/150‑1 (MQ=255)
                                                                      cacaAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGTAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGaaa                                                                               <  1:2792131/150‑1 (MQ=255)
                                                                      cacaAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGaaa                                                                               >  1:922439/1‑150 (MQ=255)
                                                                              ggCACGGACCAGCGCCTCCCCCAAGTATTCCACAAACTGATGCCCCGCCGGATGGCGACGGAAAACTTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGCCCAGTGCGCGGAAGAAAGGTGGgcg                                                                       <  2:3286637/150‑1 (MQ=255)
                                                                              ggCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGACAGGTGGgcg                                                                       >  1:3182185/1‑150 (MQ=255)
                                                                              ggCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGgcg                                                                       >  2:310256/1‑150 (MQ=255)
                                                                               gCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGgcgc                                                                      >  1:1306396/1‑150 (MQ=255)
                                                                               gCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGgcgc                                                                      >  1:2712870/1‑150 (MQ=255)
                                                                                     aaCAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGa                                                                <  2:1734209/150‑1 (MQ=255)
                                                                                      aCAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACATTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAg                                                               <  1:3179545/150‑1 (MQ=255)
                                                                                       cAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGt                                                              <  1:1172620/150‑1 (MQ=255)
                                                                                        aGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGtt                                                             <  1:309639/150‑1 (MQ=255)
                                                                                           gcCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCa                                                          <  1:2820693/150‑1 (MQ=255)
                                                                                           gcCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCa                                                          <  1:2885819/150‑1 (MQ=255)
                                                                                           gcCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCa                                                          <  2:71543/150‑1 (MQ=255)
                                                                                           gcCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCa                                                          >  2:1352804/1‑150 (MQ=255)
                                                                                              tCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATaa                                                       >  2:2248345/1‑150 (MQ=255)
                                                                                                 ccAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAAtgg                                                    <  1:459416/150‑1 (MQ=255)
                                                                                                 ccAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAAtgg                                                    <  2:3681324/150‑1 (MQ=255)
                                                                                                  cAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATTAtggc                                                   <  1:3063556/150‑1 (MQ=255)
                                                                                                  cAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAAtggc                                                   <  2:3504907/150‑1 (MQ=255)
                                                                                                    aaGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAAtggctg                                                 >  2:1559829/1‑150 (MQ=255)
                                                                                                       tATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCa                                              <  1:855687/150‑1 (MQ=255)
                                                                                                       tATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCa                                              <  1:3610049/150‑1 (MQ=255)
                                                                                                       tATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCa                                              <  1:4009902/150‑1 (MQ=255)
                                                                                                          tCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCg                                           <  2:3608488/150‑1 (MQ=255)
                                                                                                               aaaCTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTc                                      >  1:71902/1‑150 (MQ=255)
                                                                                                               aaaCTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTc                                      >  1:2112261/1‑150 (MQ=255)
                                                                                                               aaaCTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTc                                      >  1:3550039/1‑150 (MQ=255)
                                                                                                                  cTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTaa                                   <  2:1388461/150‑1 (MQ=255)
                                                                                                                   tGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAATCGCGCTGTTACCTTGATTGACATAATCGTCCAGTGCGCGGAAGAAAGGTGGGGGCAGTTGTGTTTCATAATGGGTGGCAGCGGTTTCTaag                                  >  2:131720/1‑149 (MQ=255)
                                                                                                                            gccgGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCa                         <  1:1218120/150‑1 (MQ=255)
                                                                                                                            gccgGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCa                         <  1:1475514/150‑1 (MQ=255)
                                                                                                                            gccgGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCa                         <  1:2837464/150‑1 (MQ=255)
                                                                                                                                 aTGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATaaaa                    <  2:772528/150‑1 (MQ=255)
                                                                                                                                    gCGACGGAAATATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCACAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATtc                 <  2:2171367/150‑1 (MQ=255)
                                                                                                                                    gCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATtc                 <  2:12468/150‑1 (MQ=255)
                                                                                                                                      gACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATtctc               >  1:990093/1‑150 (MQ=255)
                                                                                                                                      gACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATtctc               <  1:1156178/150‑1 (MQ=255)
                                                                                                                                      gACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATtctc               >  2:247084/1‑150 (MQ=255)
                                                                                                                                       aCGGAAAAATTCGCTGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCtcg              <  1:4071979/150‑1 (MQ=255)
                                                                                                                                          gAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAACGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCtcgtcg           >  1:50573/1‑150 (MQ=255)
                                                                                                                                          gAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAACGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCtcgtcg           >  2:1216559/1‑150 (MQ=255)
                                                                                                                                           aaaaaTTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTcgc          >  1:1099023/1‑150 (MQ=255)
                                                                                                                                           aaaaaTTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTcgc          >  1:1094737/1‑150 (MQ=255)
                                                                                                                                                ttCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTc     >  1:3433321/1‑150 (MQ=255)
                                                                                                                                                ttCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTc     <  2:1156517/150‑1 (MQ=255)
                                                                                                                                                ttCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTc     >  2:3140055/1‑150 (MQ=255)
                                                                                                                                                 tCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCg    <  2:872874/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCAGTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCA  >  NC_000913/719912‑720208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: